##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364611_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3768744 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.550759616466387 31.0 30.0 33.0 26.0 34.0 2 29.779321970396502 31.0 30.0 34.0 26.0 34.0 3 29.784542277214904 31.0 30.0 34.0 26.0 34.0 4 33.44335699108244 35.0 33.0 37.0 28.0 37.0 5 32.893811837577715 35.0 33.0 37.0 26.0 37.0 6 32.89507406180945 35.0 33.0 37.0 26.0 37.0 7 32.6868548248435 35.0 33.0 37.0 25.0 37.0 8 32.622889217203394 35.0 33.0 37.0 25.0 37.0 9 33.929353121358204 38.0 33.0 39.0 25.0 39.0 10-11 33.72502894863647 37.5 33.0 39.0 22.5 39.0 12-13 33.59370230506503 37.0 33.0 39.0 22.0 39.0 14-15 34.581223213887704 38.0 33.0 41.0 22.0 41.0 16-17 34.490724628682656 38.0 33.0 41.0 20.5 41.0 18-19 34.51871153891057 38.5 33.0 41.0 21.5 41.0 20-21 34.53615833285572 39.0 33.5 41.0 21.0 41.0 22-23 34.52744150305779 39.0 33.5 41.0 22.0 41.0 24-25 34.52035837403655 39.0 33.5 41.0 22.0 41.0 26-27 34.42926980447597 39.0 33.0 41.0 21.5 41.0 28-29 34.20432404005154 39.0 33.0 41.0 19.0 41.0 30-31 34.15452694584721 38.0 33.0 41.0 19.0 41.0 32-33 34.000637745625596 38.0 33.0 41.0 18.5 41.0 34-35 33.87670494467122 38.0 33.0 41.0 18.0 41.0 36-37 33.723160819625846 38.0 33.0 40.0 16.0 41.0 38-39 33.59202694584721 38.0 33.0 40.0 15.5 41.0 40-41 33.42767762947019 38.0 32.0 40.0 15.0 41.0 42-43 33.27219161078598 38.0 32.0 40.0 11.0 41.0 44-45 33.08374089617125 38.0 32.0 40.0 9.0 41.0 46-47 32.86088428930169 38.0 32.0 40.0 7.5 41.0 48-49 32.63436704642183 38.0 31.0 40.0 4.0 41.0 50-51 32.44668887035044 38.0 31.0 40.0 2.0 41.0 52-53 32.285854252769624 38.0 31.0 40.0 2.0 41.0 54-55 32.07501026867307 37.0 31.0 40.0 2.0 41.0 56-57 31.85431512461446 37.0 30.0 40.0 2.0 41.0 58-59 31.66398327931003 37.0 30.0 40.0 2.0 41.0 60-61 31.452194418087302 36.5 30.0 40.0 2.0 41.0 62-63 31.18593687976684 36.0 29.5 40.0 2.0 41.0 64-65 30.913662748119798 36.0 29.0 39.0 2.0 41.0 66-67 30.6328230306967 35.0 29.0 39.0 2.0 41.0 68-69 30.31456806299393 35.0 29.0 39.0 2.0 40.0 70-71 30.060170046041865 35.0 29.0 38.0 2.0 40.0 72-73 29.904888339457386 35.0 29.0 37.5 2.0 40.0 74-75 29.614288606495958 35.0 29.0 37.0 2.0 39.0 76-77 29.315059075384266 34.5 29.0 36.5 2.0 39.0 78-79 29.009850231270683 34.0 29.0 36.0 2.0 39.0 80-81 28.733114002967568 34.0 28.5 36.0 2.0 37.5 82-83 28.475331304010037 34.0 28.0 35.0 2.0 37.0 84-85 28.460473303572755 34.0 29.0 35.0 2.0 37.0 86-87 28.313938808260787 34.0 29.0 35.0 2.0 36.0 88-89 28.171864286881785 34.0 28.5 35.0 2.0 36.0 90-91 28.02098961882261 34.0 28.0 35.0 2.0 36.0 92-93 27.889954982349558 34.0 28.0 35.0 2.0 35.0 94-95 27.804994316408862 34.0 28.0 35.0 2.0 35.0 96-97 27.721977136149338 34.0 27.0 35.0 2.0 35.0 98-99 27.63743132990726 34.0 27.0 35.0 2.0 35.0 100-101 27.54180849110473 34.0 27.0 35.0 2.0 35.0 102-103 26.36205974722613 32.0 24.5 34.0 2.0 35.0 104-105 27.295537452265265 34.0 26.5 35.0 2.0 35.0 106-107 27.471588412479065 34.0 27.0 35.0 2.0 35.0 108-109 27.486974307620788 34.0 27.0 35.0 2.0 35.0 110-111 27.43068924288835 34.0 27.0 35.0 2.0 35.0 112-113 27.383968770497546 34.0 27.0 35.0 2.0 35.0 114-115 27.31146145771642 34.0 27.0 35.0 2.0 35.0 116-117 27.199641312861793 34.0 27.0 35.0 2.0 35.0 118-119 27.098725198633815 34.0 27.0 35.0 2.0 35.0 120-121 26.967524193736693 34.0 27.0 35.0 2.0 35.0 122-123 26.785879460106603 34.0 26.0 35.0 2.0 35.0 124-125 25.98201894318107 33.0 22.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7266466870072321 1101 2 0.7838116545698988 1101 3 0.6440995357017201 1101 4 0.6821956766036834 1101 5 0.9218445768129442 1101 6 1.0073583926984284 1101 7 0.9425831135165623 1101 8 1.0356989574124142 1101 9 1.0216249758035545 1101 10-11 1.1820415485400844 1101 12-13 1.1704435957330261 1101 14-15 1.3291250652079754 1101 16-17 1.4386185107544591 1101 18-19 1.3103856585481424 1101 20-21 1.3253389415074537 1101 22-23 1.2692485775543503 1101 24-25 1.136532899493254 1101 26-27 1.3013345685841315 1101 28-29 1.4636732725642645 1101 30-31 1.3522797200413166 1101 32-33 1.3464396846369908 1101 34-35 1.3515624380006201 1101 36-37 1.3463356181835593 1101 38-39 1.3558958707477942 1101 40-41 1.380583030287923 1101 42-43 1.3397771366253934 1101 44-45 1.272999676837209 1101 46-47 1.3498299621560363 1101 48-49 1.4371286498834976 1101 50-51 1.3734204926371802 1101 52-53 1.4590836530952274 1101 54-55 1.3281366789652225 1101 56-57 1.3747197489838996 1101 58-59 1.4494849509267809 1101 60-61 1.27343009512537 1101 62-63 1.3029861920266441 1101 64-65 1.4052360366744594 1101 66-67 1.3188004332190246 1101 68-69 1.3249911615221102 1101 70-71 1.3083274864900964 1101 72-73 1.2873444413038726 1101 74-75 1.336132632598673 1101 76-77 1.2903554605345562 1101 78-79 1.264877522797157 1101 80-81 1.3223876417334637 1101 82-83 1.2218744203536254 1101 84-85 1.3118984645151741 1101 86-87 1.258357857027704 1101 88-89 1.231270389258789 1101 90-91 1.2506169656973611 1101 92-93 1.2331138481258215 1101 94-95 1.1862863938434458 1101 96-97 1.2077969803562745 1101 98-99 1.2655530837434839 1101 100-101 1.2636536336650508 1101 102-103 1.3279658766658144 1101 104-105 1.2415028341120617 1101 106-107 1.2256736928794574 1101 108-109 1.199258571807185 1101 110-111 1.2850851255841285 1101 112-113 1.2109903520402412 1101 114-115 1.18828161464576 1101 116-117 1.2974681473789467 1101 118-119 1.361795140716218 1101 120-121 1.2674564196904718 1101 122-123 1.3052552905594474 1101 124-125 1.0964820054415867 1102 1 0.3242670986046612 1102 2 -0.610658347430256 1102 3 -0.005823066936745391 1102 4 0.2528181419850313 1102 5 0.6364533106848711 1102 6 0.5815839480317493 1102 7 0.636217742168661 1102 8 0.6008862148209602 1102 9 0.735788203795309 1102 10-11 0.8474766876669122 1102 12-13 0.9423966724955548 1102 14-15 0.9522233765250974 1102 16-17 1.0275600275543226 1102 18-19 0.9464260606230894 1102 20-21 0.9777753278333421 1102 22-23 0.9910398028821348 1102 24-25 0.982505990140929 1102 26-27 0.9139739544925973 1102 28-29 1.0440165168181679 1102 30-31 0.9842269122853509 1102 32-33 0.9144885337498181 1102 34-35 0.8366634911989834 1102 36-37 0.8003167993194751 1102 38-39 0.7531505109660159 1102 40-41 0.7447515213727343 1102 42-43 0.5964288800227067 1102 44-45 0.4357750969425567 1102 46-47 0.486929369211083 1102 48-49 0.5901745041967388 1102 50-51 0.49318398680919273 1102 52-53 0.5816431994220395 1102 54-55 0.6646926829858302 1102 56-57 0.3998016485093743 1102 58-59 0.6224056396473472 1102 60-61 0.5012649546651033 1102 62-63 0.6116261979111641 1102 64-65 0.5500368616665625 1102 66-67 0.566660410666536 1102 68-69 0.39398125277562457 1102 70-71 0.308431480135976 1102 72-73 0.371627531937051 1102 74-75 0.5385984324583681 1102 76-77 0.6302678866519642 1102 78-79 0.5868975160285146 1102 80-81 0.49888628256912426 1102 82-83 0.40976272066988173 1102 84-85 0.4695943657161372 1102 86-87 0.4562842617753198 1102 88-89 0.5105079129200618 1102 90-91 0.49179263379312843 1102 92-93 0.5984654481061078 1102 94-95 0.41429441005051615 1102 96-97 0.43085601323710776 1102 98-99 0.4252804845110809 1102 100-101 0.15134824128651658 1102 102-103 0.23564678575145592 1102 104-105 0.2555910290577543 1102 106-107 0.4849286291353394 1102 108-109 0.5494103409311464 1102 110-111 0.4032993590168914 1102 112-113 0.4677600847439578 1102 114-115 0.4960404827310896 1102 116-117 0.47036999404520685 1102 118-119 0.45715196936657776 1102 120-121 0.5160426768033481 1102 122-123 0.4766306590665472 1102 124-125 0.2811617666113051 1103 1 0.3263527823144976 1103 2 -0.9611685260294927 1103 3 0.08146771283003673 1103 4 0.2878867693063967 1103 5 0.5275242854447697 1103 6 0.6050974450688003 1103 7 0.5948693862570238 1103 8 0.7132029038162031 1103 9 0.7593655098819028 1103 10-11 0.974697150779221 1103 12-13 0.960744207244403 1103 14-15 0.9277782673647934 1103 16-17 0.9908674828751387 1103 18-19 1.0702123574139435 1103 20-21 1.13969405034495 1103 22-23 1.0317791266641265 1103 24-25 0.8922790759796158 1103 26-27 0.9174291456663468 1103 28-29 1.06290252649584 1103 30-31 0.970571867495984 1103 32-33 0.8989423080872285 1103 34-35 0.9782156963662842 1103 36-37 1.012205444356809 1103 38-39 0.9618261178773011 1103 40-41 1.0537255434692057 1103 42-43 1.1755658272802592 1103 44-45 1.1811121703124954 1103 46-47 1.0308449605805023 1103 48-49 1.1079629982223764 1103 50-51 1.1728795891813064 1103 52-53 1.0828190762593763 1103 54-55 1.086105595293283 1103 56-57 1.1321674438474005 1103 58-59 1.0150800118764778 1103 60-61 1.0378276318614539 1103 62-63 1.135451578892507 1103 64-65 1.109740464499943 1103 66-67 1.0539801294439854 1103 68-69 1.0438169284060415 1103 70-71 1.1013629431572092 1103 72-73 1.0424540436698386 1103 74-75 0.9567516663560873 1103 76-77 0.8643216379263059 1103 78-79 0.9519101144138062 1103 80-81 1.0281521883748468 1103 82-83 1.0169483816838927 1103 84-85 1.0298296035154806 1103 86-87 1.005043602685685 1103 88-89 0.9966433541004207 1103 90-91 0.9912302878529609 1103 92-93 1.0027109258348226 1103 94-95 0.8344010271683047 1103 96-97 0.8962521361441738 1103 98-99 0.9438664998443507 1103 100-101 0.9187745845266875 1103 102-103 0.957542617799426 1103 104-105 0.9174366236442495 1103 106-107 0.9339943636573764 1103 108-109 0.9254967473817537 1103 110-111 0.9181485052426268 1103 112-113 0.9607166674140473 1103 114-115 0.8318167386400788 1103 116-117 0.9020767750283127 1103 118-119 0.9861145756109408 1103 120-121 0.9656022658253285 1103 122-123 0.9769784413394227 1103 124-125 0.9778751506833885 1104 1 0.32642886747342104 1104 2 -0.605173757359136 1104 3 0.1512514645141323 1104 4 0.35303538777723276 1104 5 0.7169805832513063 1104 6 0.6861280488538455 1104 7 0.7633522355222837 1104 8 0.7953823844540224 1104 9 0.739130928751969 1104 10-11 0.9202407588840202 1104 12-13 0.8983426610781748 1104 14-15 0.9821435108680632 1104 16-17 1.1518565772753817 1104 18-19 0.9357438262718603 1104 20-21 1.024832963208354 1104 22-23 0.974341843090663 1104 24-25 0.9760837120899026 1104 26-27 0.8741942743090689 1104 28-29 1.103474191116078 1104 30-31 1.012476605620094 1104 32-33 1.047534995009741 1104 34-35 1.0298944823764629 1104 36-37 1.054667612572132 1104 38-39 1.0219013218597084 1104 40-41 1.108821964445049 1104 42-43 1.0948688311331907 1104 44-45 1.0275078145864214 1104 46-47 1.1535750263593343 1104 48-49 1.1126340479732022 1104 50-51 1.158620859472343 1104 52-53 0.9669819097177381 1104 54-55 1.2359530992839112 1104 56-57 1.2449329321983171 1104 58-59 1.1374318683724454 1104 60-61 1.1594319810736948 1104 62-63 1.122440202487553 1104 64-65 1.105385516467642 1104 66-67 1.0267644409556276 1104 68-69 1.0433239128607852 1104 70-71 1.1139703263669425 1104 72-73 1.1345187144264663 1104 74-75 1.0491170137187638 1104 76-77 1.0292944824907515 1104 78-79 1.1087844582810895 1104 80-81 1.036598183834709 1104 82-83 1.0604557030484223 1104 84-85 1.0330595135051972 1104 86-87 1.0638406483740503 1104 88-89 1.1064738642310559 1104 90-91 1.1157322059950978 1104 92-93 1.1004093814417963 1104 94-95 1.0633681851587298 1104 96-97 1.1010710769880951 1104 98-99 1.1078445377410908 1104 100-101 1.1391920233536759 1104 102-103 1.1549219499942858 1104 104-105 1.161132049958173 1104 106-107 1.1770177345574169 1104 108-109 1.208459398525239 1104 110-111 1.0991991119154676 1104 112-113 1.127744987377742 1104 114-115 1.1598321626669232 1104 116-117 1.1335420774817457 1104 118-119 1.0484825574561683 1104 120-121 1.0544921227418023 1104 122-123 1.128040729481608 1104 124-125 1.047719110064591 1105 1 0.5885377829285012 1105 2 0.37746857614683904 1105 3 0.5834590866026339 1105 4 0.4693463652861425 1105 5 0.6350913639164801 1105 6 0.5967075988185186 1105 7 0.7044282374942767 1105 8 0.7059257158829908 1105 9 0.7702501259997163 1105 10-11 0.9355664943338695 1105 12-13 0.9147057416648607 1105 14-15 0.998239223910474 1105 16-17 1.0675283020785784 1105 18-19 1.1141194026983072 1105 20-21 1.106740425294575 1105 22-23 0.992448574754917 1105 24-25 1.0756017030023415 1105 26-27 1.0526854919815847 1105 28-29 1.2556417859383444 1105 30-31 1.0512857900000299 1105 32-33 1.0121795639201707 1105 34-35 1.1084778036962746 1105 36-37 1.1119175044504601 1105 38-39 1.118689040305533 1105 40-41 1.0122758957134792 1105 42-43 1.1139066550310162 1105 44-45 1.2210919724031228 1105 46-47 1.2278373517851051 1105 48-49 1.1673912236037367 1105 50-51 1.08769370945285 1105 52-53 1.0950861585880247 1105 54-55 1.0972406459809037 1105 56-57 1.042735392567053 1105 58-59 0.9182730193921742 1105 60-61 0.9182339751399127 1105 62-63 0.9854640141725213 1105 64-65 1.0364005965488694 1105 66-67 0.9609572303524061 1105 68-69 0.9195125393506807 1105 70-71 0.8603825111010508 1105 72-73 0.7858468757173043 1105 74-75 0.8996377330349006 1105 76-77 0.9063154233895752 1105 78-79 0.9337676393541869 1105 80-81 0.7032825593185876 1105 82-83 0.7814213408239112 1105 84-85 0.7027538094862749 1105 86-87 0.8087363710272761 1105 88-89 0.7727051075680009 1105 90-91 0.7422256644114178 1105 92-93 0.5821345494975851 1105 94-95 0.6590922177144805 1105 96-97 0.7666192850159739 1105 98-99 0.8421866952267614 1105 100-101 0.8139955800672745 1105 102-103 0.4155705109526622 1105 104-105 0.4275361989827644 1105 106-107 0.7227107989303043 1105 108-109 0.8208927234936034 1105 110-111 0.7408930092256831 1105 112-113 0.5883132802299684 1105 114-115 0.7047592539536538 1105 116-117 0.45403629586438754 1105 118-119 0.33000162691566715 1105 120-121 0.37248218978614034 1105 122-123 0.4510548502610803 1105 124-125 0.29918026546257437 1106 1 0.45960500348347466 1106 2 0.3583037450975546 1106 3 0.5297018611691087 1106 4 0.4401706437283579 1106 5 0.5186156507360096 1106 6 0.6032409183539187 1106 7 0.5549764587933552 1106 8 0.5859256173263532 1106 9 0.6262381809351822 1106 10-11 0.719149422353027 1106 12-13 0.6164598034303026 1106 14-15 0.7022352027996419 1106 16-17 0.8535374481345954 1106 18-19 0.7795518938651753 1106 20-21 0.8751017733522701 1106 22-23 0.865888792762199 1106 24-25 0.8319487504918612 1106 26-27 0.8698634212080592 1106 28-29 1.0311610562249953 1106 30-31 0.9430412808367237 1106 32-33 0.7993445329487514 1106 34-35 0.8018089687835683 1106 36-37 0.8559158979772619 1106 38-39 0.9517000934324855 1106 40-41 0.9313429092995094 1106 42-43 0.8839225226497334 1106 44-45 0.7775016033577984 1106 46-47 0.7781278474757158 1106 48-49 0.7499582790763384 1106 50-51 0.8798377598350129 1106 52-53 0.9766033394739466 1106 54-55 1.0874005048477997 1106 56-57 0.8504168969355774 1106 58-59 0.8644795743941671 1106 60-61 0.9912614872254153 1106 62-63 0.9942394972232407 1106 64-65 0.9028351623329627 1106 66-67 0.7515541879089902 1106 68-69 0.807885073586796 1106 70-71 0.8392655194438703 1106 72-73 0.8812947909980196 1106 74-75 0.9608115918722255 1106 76-77 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3 44881.0 4 20787.0 5 15793.0 6 16864.0 7 19757.0 8 22439.0 9 24358.0 10 26498.0 11 29190.0 12 32101.0 13 34766.0 14 36767.0 15 30580.0 16 21738.0 17 18443.0 18 17006.0 19 16220.0 20 15758.0 21 16666.0 22 19784.0 23 26322.0 24 36135.0 25 48152.0 26 58871.0 27 65894.0 28 76241.0 29 89746.0 30 103981.0 31 122894.0 32 149172.0 33 184415.0 34 237488.0 35 321474.0 36 511351.0 37 800117.0 38 314390.0 39 1029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.51598171576279 19.66691826918354 13.99991451001361 24.817185505040058 2 15.45419710398776 20.806314941397563 38.87230837333591 24.867179581278766 3 19.33060457277013 24.62157153683031 29.165472634914973 26.882351255484586 4 13.00143003205299 17.28781113280882 36.326449595814935 33.38430923932326 5 13.338547951037446 38.544447493825004 34.09850372145342 14.018500833684131 6 32.32481499072632 37.42316377135003 16.727332549691805 13.524688688231844 7 28.46754780903134 32.453438068491785 20.635070994474553 18.443943128002328 8 25.858561897544647 36.80196903796066 18.948142935683613 18.391326128811084 9 25.795857652956578 16.419496950301333 19.57814469743545 38.20650069930664 10-11 24.744413468817815 27.21794284817679 27.170220530207562 20.867423152797834 12-13 25.596682429240904 24.1989348321047 28.030859086832148 22.17352365182225 14-15 22.48848075455436 25.6651627872912 25.82086233267772 26.025494125476715 16-17 22.26087203535801 28.844052450827352 26.33740742628616 22.557668087528487 18-19 22.173810829664337 27.52127301929212 28.57832363646109 21.726592514582457 20-21 22.80423236897706 26.70984341837593 28.71043465292568 21.775489559721322 22-23 22.483355142570314 26.491217082802475 28.608017820327092 22.417409954300116 24-25 22.29635390464303 26.70060635585755 28.694068899346835 22.308970840152583 26-27 22.2370901287007 26.898324746918334 28.58441698348309 22.280168140897867 28-29 22.156508179102005 26.803060030256248 28.708948689022094 22.331483101619654 30-31 22.285872959267067 26.66002254331947 28.690168395624642 22.36393610178882 32-33 22.115269163355453 26.702451539769807 28.650174473147615 22.532104823727128 34-35 22.461501762921547 26.736414041388855 28.548662366029635 22.25342182965996 36-37 22.44836037310628 26.628393028595642 28.465984042140363 22.457262556157715 38-39 22.402105316784585 26.800865221941315 28.497358801765255 22.299670659508845 40-41 22.51302058630416 26.648815447376563 28.470403995389976 22.367759970929303 42-43 22.411651558166113 26.66147291229781 28.279846596733186 22.647028932802886 44-45 22.510921410422146 26.861349563674263 28.044130882861772 22.58359814304182 46-47 22.534410386923902 27.096507226489713 27.65975935979639 22.70932302679 48-49 22.73859073099879 27.090741877776463 27.49983150840574 22.670835882819006 50-51 22.690732681705736 27.245253490776832 27.38726468450669 22.67674914301074 52-53 22.85540639128982 27.222504294208793 27.24117101783528 22.680918296666114 54-55 22.73022524214964 27.149708231707965 27.354458143084276 22.765608383058122 56-57 22.618164556265075 27.35149984430866 27.411786292501517 22.618549306924756 58-59 22.701541946070098 27.07837146805408 27.375287363641576 22.84479922223425 60-61 22.752354630614338 27.062703118067983 27.51638211563322 22.668560135684462 62-63 22.661860290855522 27.16289564905443 27.52777848535215 22.647465574737897 64-65 22.585222731014646 26.97706321298104 27.54006491581044 22.89764914019387 66-67 22.56842067277586 27.22113786449809 27.412156410729942 22.798285051996103 68-69 22.521488047808237 27.048021305890618 27.574632043798392 22.855858602502757 70-71 22.50861294903554 26.9295287766959 27.48739367810602 23.074464596162542 72-73 22.71920559551745 27.08326395632187 27.30152458719075 22.89600586096993 74-75 22.562179797179727 27.15863883410517 27.328038274083994 22.951143094631107 76-77 22.700801278734513 27.035449114634496 27.40850621668967 22.85524338994132 78-79 22.687960296139135 27.095645542635836 27.3382091948646 22.87818496636043 80-81 22.5234365256893 27.226383703621153 27.32324760062515 22.926932170064397 82-83 22.67983518609889 27.237026848925854 27.239176110926334 22.843961854048924 84-85 22.666178616531766 27.092726120424647 27.29543320662869 22.945662056414896 86-87 22.553329071942937 27.00267438068545 27.52798494344105 22.91601160393056 88-89 22.691541623886053 27.02585092352398 27.3714568263932 22.91115062619677 90-91 22.581249880596825 27.21089572547247 27.42714814272341 22.7807062512073 92-93 22.555612755309184 27.115619866948652 27.38269111867681 22.94607625906535 94-95 22.509301712486298 27.118463940747134 27.34060646780645 23.03162787896012 96-97 22.656943247385694 27.03122147403102 27.251786919768524 23.060048358814765 98-99 22.437489668179744 27.014084708852952 27.21112893925938 23.337296683707923 100-101 22.333338374820794 27.09266174352149 27.379097833778975 23.19490204787874 102-103 22.46450472452206 26.975507205191334 27.3051663672298 23.254821703056802 104-105 22.39941019793978 26.90105558329853 27.443109361741197 23.256424857020498 106-107 22.380523900526935 26.98053483303251 27.34320478440409 23.29573648203646 108-109 22.34071332863744 26.96780599719759 27.145553713258185 23.54592696090678 110-111 22.306492041251733 27.015246180096685 27.325471324506008 23.352790454145573 112-113 22.41290212503544 26.998055919415588 27.270741978594547 23.318299976954428 114-115 22.277789209585368 27.113762229104303 27.16554367399368 23.44290488731665 116-117 22.130250257352714 26.965973719309556 27.2999783344772 23.60379768886054 118-119 22.126676775491095 27.034423028165623 27.16781280958496 23.671087386758323 120-121 22.10725847894648 27.065847780538093 27.211497418125653 23.615396322389774 122-123 22.090094765860833 27.07255626892972 27.122838732993348 23.714510232216092 124-125 22.044134332587888 26.97156773360193 27.148115147070218 23.83618278673996 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 104.0 1 107.5 2 162.0 3 269.5 4 259.5 5 254.5 6 602.0 7 1196.5 8 1828.0 9 2165.5 10 1939.5 11 1674.5 12 1738.5 13 1999.0 14 2357.5 15 2654.0 16 2965.0 17 3316.0 18 3650.0 19 4063.0 20 4633.0 21 5446.0 22 6624.0 23 7937.0 24 9631.0 25 12577.0 26 16683.0 27 21877.0 28 27638.0 29 34151.0 30 41768.5 31 49137.5 32 56324.0 33 64018.5 34 72988.0 35 81801.0 36 90434.0 37 99780.0 38 106416.0 39 110775.0 40 115411.0 41 120620.0 42 125932.0 43 132540.0 44 140452.5 45 144709.5 46 144352.5 47 145553.0 48 150307.0 49 149891.0 50 145350.5 51 140702.5 52 134454.0 53 130005.5 54 125730.0 55 116903.5 56 106214.0 57 96485.5 58 87707.0 59 77958.0 60 65686.5 61 52762.0 62 41360.0 63 31995.5 64 23627.0 65 17199.0 66 12652.0 67 9407.0 68 7082.5 69 5285.0 70 3850.5 71 3032.5 72 2501.5 73 2177.5 74 1712.0 75 1112.5 76 733.5 77 466.0 78 314.5 79 189.0 80 123.0 81 103.5 82 73.0 83 42.5 84 31.5 85 21.0 86 12.0 87 6.5 88 5.5 89 2.0 90 2.0 91 1.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05893210045574865 2 0.0781427446385321 3 0.0 4 0.007880609561169451 5 0.029983463986941006 6 3.714765449709505E-4 7 0.0 8 0.0 9 2.9187442819146115E-4 10-11 0.05737985917854861 12-13 0.008597028612184855 14-15 0.007111122432301053 16-17 0.05349262247581688 18-19 7.96021167794894E-5 20-21 0.009499185935685734 22-23 0.012285260022967863 24-25 0.0 26-27 0.0 28-29 0.050905553680483474 30-31 0.0 32-33 0.0029850793792308527 34-35 0.0 36-37 1.4593721409573057E-4 38-39 0.0 40-41 1.3267019463248233E-5 42-43 0.0030514144765470936 44-45 0.0 46-47 1.4593721409573057E-4 48-49 2.653403892649647E-4 50-51 7.164190510154046E-4 52-53 1.4593721409573057E-4 54-55 0.0 56-57 0.0018441157053915044 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.378116422871917E-4 66-67 0.0 68-69 5.572148174564258E-4 70-71 0.0 72-73 0.0022421262892889514 74-75 0.003621896313466768 76-77 4.7761270067693643E-4 78-79 0.0011276966543760998 80-81 0.0014328381020308092 82-83 2.2553933087521993E-4 84-85 1.4593721409573057E-4 86-87 5.572148174564258E-4 88-89 1.3267019463248233E-5 90-91 0.0 92-93 0.007270326665860033 94-95 0.005771153466512981 96-97 0.006580441653771123 98-99 0.001247099829545334 100-101 3.98010583897447E-4 102-103 7.96021167794894E-5 104-105 5.837488563829223E-4 106-107 0.004404650461798413 108-109 0.0053200748047625415 110-111 7.827541483316458E-4 112-113 0.00330348784634881 114-115 5.174137590666811E-4 116-117 0.002215592250362455 118-119 0.0023747964839214335 120-121 0.012059720692092643 122-123 9.154243429641281E-4 124-125 0.0018043146470017595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3768744.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.18166009897167 #Duplication Level Percentage of deduplicated Percentage of total 1 74.62620561509073 38.941192958826356 2 12.816739221049525 13.375974592199306 3 4.988799825522157 7.809715703916191 4 2.3853478640093706 4.978856458301803 5 1.391795200983632 3.631309205255393 6 0.8507496199901553 2.663611649979336 7 0.5706827103390671 2.0845419850691176 8 0.3972567183794993 1.6583612040409559 9 0.29057984135832543 1.3646644662035923 >10 1.5279499106423657 14.223165825021924 >50 0.10282358756108477 3.728285956602073 >100 0.049379493745355975 4.467904822727618 >500 0.0013830474601558825 0.4932131565441318 >1k 2.0489591241137274E-4 0.22300440323735732 >5k 1.0244795620568577E-4 0.3561976120749149 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7328 0.1944414372533661 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5998 0.15915116548112582 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3941 0.10457064740932258 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.3068077852992933E-5 0.0 0.0 0.0 3 0.0 5.3068077852992933E-5 0.0 0.0 0.0 4 0.0 5.3068077852992933E-5 0.0 0.0 0.0 5 0.0 5.3068077852992933E-5 0.0 0.0 0.0 6 0.0 5.3068077852992933E-5 0.0 0.0 0.0 7 0.0 5.3068077852992933E-5 0.0 0.0 0.0 8 0.0 5.3068077852992933E-5 0.0 0.0 0.0 9 0.0 5.3068077852992933E-5 0.0 2.6534038926496467E-5 0.0 10-11 0.0 5.3068077852992933E-5 0.0 2.6534038926496467E-5 0.0 12-13 0.0 5.3068077852992933E-5 0.0 2.6534038926496467E-5 0.0 14-15 0.0 5.3068077852992933E-5 0.0 2.6534038926496467E-5 0.0 16-17 0.0 5.3068077852992933E-5 0.0 2.6534038926496467E-5 0.0 18-19 0.0 5.3068077852992933E-5 0.0 2.6534038926496467E-5 0.0 20-21 0.0 7.96021167794894E-5 0.0 5.3068077852992933E-5 0.0 22-23 0.0 7.96021167794894E-5 0.0 1.1940317516923411E-4 0.0 24-25 0.0 7.96021167794894E-5 0.0 1.8573827248547528E-4 0.0 26-27 0.0 7.96021167794894E-5 0.0 2.2553933087522E-4 0.0 28-29 0.0 7.96021167794894E-5 0.0 6.102828953094187E-4 0.0 30-31 0.0 7.96021167794894E-5 0.0 0.001990052919487235 0.0 32-33 0.0 7.96021167794894E-5 0.0 0.00480266104569586 0.0 34-35 0.0 7.96021167794894E-5 0.0 0.008437824378625877 0.0 36-37 0.0 9.286913624273764E-5 0.0 0.014328381020308091 0.0 38-39 0.0 1.3267019463248235E-4 0.0 0.024822593415737445 0.0 40-41 0.0 1.3267019463248235E-4 0.0 0.03778447143133097 0.0 42-43 0.0 1.4593721409573057E-4 0.0 0.05078615050531424 0.0 44-45 0.0 1.592042335589788E-4 0.0 0.06518086662293857 1.3267019463248233E-5 46-47 0.0 1.592042335589788E-4 0.0 0.08250759404194076 2.6534038926496467E-5 48-49 0.0 1.592042335589788E-4 0.0 0.10258059448983534 2.6534038926496467E-5 50-51 1.3267019463248233E-5 1.592042335589788E-4 0.0 0.12603668490085823 2.6534038926496467E-5 52-53 2.6534038926496467E-5 1.592042335589788E-4 0.0 0.14779459682058532 2.6534038926496467E-5 54-55 2.6534038926496467E-5 1.8573827248547528E-4 0.0 0.17257738917793303 2.6534038926496467E-5 56-57 2.6534038926496467E-5 1.8573827248547528E-4 0.0 0.20354261260515438 2.6534038926496467E-5 58-59 2.6534038926496467E-5 2.1227231141197173E-4 0.0 0.23720104098341516 2.6534038926496467E-5 60-61 2.6534038926496467E-5 2.1227231141197173E-4 0.0 0.27515798366776834 2.6534038926496467E-5 62-63 2.6534038926496467E-5 2.1227231141197173E-4 0.0 0.3151580473494618 2.6534038926496467E-5 64-65 2.6534038926496467E-5 2.1227231141197173E-4 0.0 0.35747983943722367 2.6534038926496467E-5 66-67 2.6534038926496467E-5 2.3880635033846821E-4 0.0 0.4067137486653378 2.6534038926496467E-5 68-69 2.6534038926496467E-5 2.653403892649647E-4 0.0 0.46656127346405063 2.6534038926496467E-5 70-71 3.98010583897447E-5 2.653403892649647E-4 0.0 0.5341169365709106 2.6534038926496467E-5 72-73 5.3068077852992933E-5 2.653403892649647E-4 0.0 0.6038483908697434 2.6534038926496467E-5 74-75 5.3068077852992933E-5 2.653403892649647E-4 0.0 0.6772813435988223 2.6534038926496467E-5 76-77 5.3068077852992933E-5 2.653403892649647E-4 0.0 0.758661240986387 2.6534038926496467E-5 78-79 5.3068077852992933E-5 2.653403892649647E-4 0.0 0.8541970481412375 2.6534038926496467E-5 80-81 5.3068077852992933E-5 2.653403892649647E-4 0.0 0.9637693618882046 2.6534038926496467E-5 82-83 5.3068077852992933E-5 2.653403892649647E-4 0.0 1.0827877934930046 3.98010583897447E-5 84-85 5.3068077852992933E-5 2.653403892649647E-4 0.0 1.2053617863139552 5.3068077852992933E-5 86-87 5.3068077852992933E-5 2.653403892649647E-4 0.0 1.3353785770537878 5.3068077852992933E-5 88-89 5.3068077852992933E-5 2.653403892649647E-4 0.0 1.4842876035092858 5.3068077852992933E-5 90-91 5.3068077852992933E-5 2.653403892649647E-4 0.0 1.6537605101328188 5.3068077852992933E-5 92-93 5.3068077852992933E-5 2.653403892649647E-4 0.0 1.8370709180565195 5.3068077852992933E-5 94-95 5.3068077852992933E-5 2.653403892649647E-4 0.0 2.0276914537044703 5.3068077852992933E-5 96-97 5.3068077852992933E-5 2.653403892649647E-4 0.0 2.223180985495433 5.3068077852992933E-5 98-99 5.3068077852992933E-5 3.051414476547094E-4 0.0 2.443731917052472 5.3068077852992933E-5 100-101 5.3068077852992933E-5 3.184084671179576E-4 0.0 2.7025051316831283 5.3068077852992933E-5 102-103 5.3068077852992933E-5 3.184084671179576E-4 0.0 2.9826913157274677 5.3068077852992933E-5 104-105 5.3068077852992933E-5 3.184084671179576E-4 0.0 3.268953794685975 5.3068077852992933E-5 106-107 5.3068077852992933E-5 3.184084671179576E-4 0.0 3.5543008493015176 5.3068077852992933E-5 108-109 5.3068077852992933E-5 3.184084671179576E-4 0.0 3.8648817749361593 5.3068077852992933E-5 110-111 5.3068077852992933E-5 3.184084671179576E-4 0.0 4.215953643972634 5.3068077852992933E-5 112-113 5.3068077852992933E-5 3.184084671179576E-4 0.0 4.596557898334299 5.3068077852992933E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7120 0.0 48.70163 1 GTATCAA 11520 0.0 46.288284 1 ATCAACG 14580 0.0 36.11586 3 TCAACGC 14890 0.0 35.3663 4 TATCAAC 15150 0.0 34.860577 2 CAACGCA 15090 0.0 34.341625 5 AACGCAG 15455 0.0 33.790493 6 GTACATG 13580 0.0 31.720428 1 TACATGG 14115 0.0 29.10662 2 ACGCAGA 18195 0.0 28.30885 7 CGCAGAG 18505 0.0 27.770256 8 ACATGGG 14595 0.0 27.150782 3 GCAGAGT 20970 0.0 23.768541 9 GAGTACT 11900 0.0 22.447098 12-13 GGACCGA 850 0.0 21.717342 6 AGTACTT 12665 0.0 21.632034 12-13 CATGGGG 7305 0.0 21.504272 4 GTACTTT 12475 0.0 21.340849 14-15 AGAGTAC 19785 0.0 21.12618 10-11 CATGGGA 8445 0.0 20.292421 4 >>END_MODULE