FastQCFastQC Report
Thu 26 May 2016
SRR1364610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808961
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26150.1445581192739921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42750.043.8943671
ATCAACG51200.035.8356363
TCAACGC51500.035.3955424
TACATGG68850.034.9551962
AACGCAG53450.034.6326646
GTACATG69700.034.6143841
GGTATCA28300.034.5216671
CAACGCA53550.034.4864585
TATCAAC56250.032.7242972
ACATGGG77150.030.5769123
ACGCAGA63100.028.5768497
CGCAGAG61950.027.1807828
CATGGGA51950.024.5393244
GCAGAGT68000.022.1368839
GTCGTAC1350.002538222822.0633071
AGTACAT50750.021.9502722
TACCTGG18550.021.8373222
GAGTACA57150.021.4726371
CATGGGG33800.021.325694
GTACCTG20500.020.6318841
Produced by FastQC (version 0.11.5)