FastQCFastQC Report
Thu 26 May 2016
SRR1364610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808961
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51060.2822614749571716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44340.2451130787230902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.1508047989978778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.043.1711731
GTATCAA67600.042.1299551
ATCAACG85000.033.321233
TCAACGC85800.032.8070264
GGACCGA3850.032.483596
CAACGCA87150.031.9226655
TATCAAC88650.031.7018472
AACGCAG89600.031.3053326
GTACATG77600.029.8674141
TACATGG76500.029.4984422
AGGACCG6100.027.3449975
ACATGGG80900.027.2136123
ACGCAGA105750.026.2443037
CGCAGAG105950.026.0261238
GCAGAGT113750.023.090259
GAGTACT59250.023.07065212-13
CTATACG1300.00205052322.8886244
CATGGGA53700.022.8289554
GTACCTG18350.022.4038561
AGTACTT63350.022.0476212-13
Produced by FastQC (version 0.11.5)