##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364610_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1808961 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.687792053007225 31.0 30.0 33.0 26.0 34.0 2 29.7046431625668 31.0 30.0 34.0 26.0 34.0 3 29.744027649020627 31.0 30.0 34.0 26.0 34.0 4 33.45937087643128 35.0 33.0 37.0 28.0 37.0 5 32.93785051197898 35.0 33.0 37.0 26.0 37.0 6 32.974447763108216 35.0 33.0 37.0 26.0 37.0 7 32.76243932290414 35.0 33.0 37.0 25.0 37.0 8 32.70365806670238 35.0 33.0 37.0 25.0 37.0 9 33.99346862646569 38.0 34.0 39.0 25.0 39.0 10-11 33.808655078799376 37.5 33.0 39.0 24.0 39.0 12-13 33.69971298441481 37.0 33.0 39.0 23.0 39.0 14-15 34.69784285012226 38.0 33.0 41.0 23.5 41.0 16-17 34.618443681207054 38.0 33.0 41.0 23.0 41.0 18-19 34.65150133142727 38.5 33.5 41.0 22.5 41.0 20-21 34.663378867758894 39.0 34.0 41.0 22.5 41.0 22-23 34.66418927771245 39.0 34.0 41.0 23.0 41.0 24-25 34.670551769772814 39.0 34.0 41.0 23.0 41.0 26-27 34.58088316995226 39.0 34.0 41.0 23.0 41.0 28-29 34.348997573745365 39.0 33.0 41.0 20.5 41.0 30-31 34.291568751344 38.5 33.0 41.0 21.5 41.0 32-33 34.12381858978718 38.0 33.0 41.0 19.5 41.0 34-35 34.00962624401521 38.0 33.0 41.0 19.0 41.0 36-37 33.85684683086036 38.0 33.0 40.0 18.0 41.0 38-39 33.72894329949623 38.0 33.0 40.0 16.5 41.0 40-41 33.568129440048736 38.0 33.0 40.0 15.5 41.0 42-43 33.40153823106192 38.0 32.5 40.0 15.0 41.0 44-45 33.22254238759155 38.0 32.0 40.0 11.0 41.0 46-47 32.99473288810538 38.0 32.0 40.0 8.5 41.0 48-49 32.75360192950539 38.0 31.5 40.0 7.0 41.0 50-51 32.56523855406501 38.0 31.0 40.0 2.0 41.0 52-53 32.40871417349517 38.0 31.0 40.0 2.0 41.0 54-55 32.20037717783855 37.5 31.0 40.0 2.0 41.0 56-57 31.986502472966528 37.0 31.0 40.0 2.0 41.0 58-59 31.799443437420706 37.0 30.0 40.0 2.0 41.0 60-61 31.580863821829215 36.5 30.0 40.0 2.0 41.0 62-63 31.312967499022918 36.0 30.0 40.0 2.0 41.0 64-65 31.047809764831857 36.0 29.0 39.0 2.0 41.0 66-67 30.772228367554636 35.0 29.0 39.0 2.0 41.0 68-69 30.45880591123855 35.0 29.0 39.0 2.0 40.5 70-71 30.20049022615745 35.0 29.0 38.0 2.0 40.0 72-73 30.036866466441232 35.0 29.0 37.5 2.0 40.0 74-75 29.737564823122224 35.0 29.0 37.0 2.0 39.0 76-77 29.43542757417103 34.5 29.0 36.5 2.0 39.0 78-79 29.123930808900802 34.0 29.0 36.0 2.0 39.0 80-81 28.838793926458337 34.0 28.5 36.0 2.0 37.5 82-83 28.580057557902023 34.0 28.0 35.0 2.0 37.0 84-85 28.554676413698253 34.0 29.0 35.0 2.0 37.0 86-87 28.402388719270345 34.0 29.0 35.0 2.0 36.0 88-89 28.25483523414822 34.0 29.0 35.0 2.0 36.0 90-91 28.101096706894175 34.0 28.5 35.0 2.0 36.0 92-93 27.96674223490722 34.0 28.0 35.0 2.0 35.5 94-95 27.88059775749726 34.0 28.0 35.0 2.0 35.0 96-97 27.796555868258075 34.0 28.0 35.0 2.0 35.0 98-99 27.711546296465208 34.0 27.0 35.0 2.0 35.0 100-101 27.61641295749328 34.0 27.0 35.0 2.0 35.0 102-103 26.427230603644855 32.0 24.5 34.0 2.0 35.0 104-105 27.359997258094566 34.0 26.5 35.0 2.0 35.0 106-107 27.536726330750085 34.0 27.0 35.0 2.0 35.0 108-109 27.549747893956805 34.0 27.0 35.0 2.0 35.0 110-111 27.491233641852975 34.0 27.0 35.0 2.0 35.0 112-113 27.437879534163535 34.0 27.0 35.0 2.0 35.0 114-115 27.355202516803843 34.0 27.0 35.0 2.0 35.0 116-117 27.245178585939662 34.0 27.0 35.0 2.0 35.0 118-119 27.152865650503244 34.0 27.0 35.0 2.0 35.0 120-121 27.020771868492467 34.0 27.0 35.0 2.0 35.0 122-123 26.83467139424233 34.0 26.5 35.0 2.0 35.0 124-125 26.02880244516051 33.0 22.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6806241281052223 1101 2 0.7307058073928019 1101 3 0.6463932257623277 1101 4 0.7554982280464273 1101 5 0.9526716720042501 1101 6 0.9439726285762333 1101 7 0.914657489207535 1101 8 1.0195522538738686 1101 9 1.086991380456908 1101 10-11 1.2817863896160873 1101 12-13 1.1555109754325699 1101 14-15 1.231543733376867 1101 16-17 1.4090669957444248 1101 18-19 1.3300503283735097 1101 20-21 1.365378573584799 1101 22-23 1.2488695246447747 1101 24-25 1.1228189500041665 1101 26-27 1.2097147655937022 1101 28-29 1.3823557133267315 1101 30-31 1.3451331204439825 1101 32-33 1.2982594410519184 1101 34-35 1.2550855681532767 1101 36-37 1.3617243357560724 1101 38-39 1.4237324062227756 1101 40-41 1.445745693066634 1101 42-43 1.405538626900018 1101 44-45 1.348994528885477 1101 46-47 1.4523789281227337 1101 48-49 1.602237658923812 1101 50-51 1.4887708316891661 1101 52-53 1.5295658907180325 1101 54-55 1.4462809139457136 1101 56-57 1.4800461608825906 1101 58-59 1.4588627462463641 1101 60-61 1.497388773908927 1101 62-63 1.4978505775746491 1101 64-65 1.4731825543937624 1101 66-67 1.4878302859848667 1101 68-69 1.5147957882631253 1101 70-71 1.391705590794487 1101 72-73 1.3415320659356773 1101 74-75 1.4008212844811538 1101 76-77 1.3589139189069535 1101 78-79 1.2558001251666298 1101 80-81 1.2559509709652374 1101 82-83 1.2807147709861972 1101 84-85 1.2816814979051898 1101 86-87 1.3318277168667443 1101 88-89 1.3599038762907583 1101 90-91 1.393658850240346 1101 92-93 1.3405142800331866 1101 94-95 1.27373266366779 1101 96-97 1.2715273512371752 1101 98-99 1.3139725408044036 1101 100-101 1.351719566250587 1101 102-103 1.3567566547354701 1101 104-105 1.3260209585454028 1101 106-107 1.2917174173441133 1101 108-109 1.3325266140223242 1101 110-111 1.3760374421811612 1101 112-113 1.302787130175897 1101 114-115 1.2741832311227412 1101 116-117 1.312067876306532 1101 118-119 1.2687289277607583 1101 120-121 1.3437466982761777 1101 122-123 1.371851291138249 1101 124-125 1.275107824400294 1102 1 0.3967955984309164 1102 2 -0.5645541842139714 1102 3 0.05875796314933979 1102 4 0.2616694939525672 1102 5 0.6406697191963033 1102 6 0.6054843151617817 1102 7 0.518343293953194 1102 8 0.5563295355668103 1102 9 0.8191340300308951 1102 10-11 0.8460296424682383 1102 12-13 0.9821018705857796 1102 14-15 1.0159259995399452 1102 16-17 1.1513340245944335 1102 18-19 1.1127427262509073 1102 20-21 1.063628862477728 1102 22-23 1.1004605300242147 1102 24-25 0.9985599207725713 1102 26-27 0.9941375148442617 1102 28-29 1.2022446533873108 1102 30-31 1.1243678217449329 1102 32-33 1.1430460811520575 1102 34-35 0.9771164643646557 1102 36-37 0.9698021546716191 1102 38-39 0.9125149835845505 1102 40-41 0.9172596282672387 1102 42-43 0.9087536253628627 1102 44-45 0.6832505559554392 1102 46-47 0.7248002456348033 1102 48-49 0.8439541578662499 1102 50-51 0.7030935075490987 1102 52-53 0.735464677387796 1102 54-55 0.8263563808339498 1102 56-57 0.762491652008574 1102 58-59 0.9200595064770631 1102 60-61 0.6385348621404212 1102 62-63 0.764599052143847 1102 64-65 0.6422982474421737 1102 66-67 0.7421883304352939 1102 68-69 0.45941131717466277 1102 70-71 0.352554436966372 1102 72-73 0.43267532836749467 1102 74-75 0.38993754669772684 1102 76-77 0.4355955001843661 1102 78-79 0.42610165202034267 1102 80-81 0.38229908784030897 1102 82-83 0.39472456468678985 1102 84-85 0.5229743273082583 1102 86-87 0.5052965442881465 1102 88-89 0.5482167696731359 1102 90-91 0.4444713377897962 1102 92-93 0.4847539174650528 1102 94-95 0.4924447483503904 1102 96-97 0.42593024774195953 1102 98-99 0.44452929548771536 1102 100-101 0.2965597562565563 1102 102-103 0.315073504237386 1102 104-105 0.3391158344687497 1102 106-107 0.5206697190481293 1102 108-109 0.6088573552355925 1102 110-111 0.5097480698734351 1102 112-113 0.4711602992681385 1102 114-115 0.5391568630048127 1102 116-117 0.6062574710307409 1102 118-119 0.5978674516672235 1102 120-121 0.6488939825624485 1102 122-123 0.6537618844399447 1102 124-125 0.4509350922849009 1103 1 0.340432298393921 1103 2 -0.6720643580948433 1103 3 0.07223141856924542 1103 4 0.33152243512903823 1103 5 0.6389031635174334 1103 6 0.6746112112032208 1103 7 0.6790813627656291 1103 8 0.7752760764473194 1103 9 0.7332040448292645 1103 10-11 0.8698619584134875 1103 12-13 0.8273051169786854 1103 14-15 0.918832492325734 1103 16-17 1.1115460575208118 1103 18-19 1.095849459510255 1103 20-21 1.024392365992341 1103 22-23 0.9789924852591412 1103 24-25 0.8736281320193342 1103 26-27 0.9261944885771598 1103 28-29 0.9522550584927458 1103 30-31 0.9469758036169296 1103 32-33 1.0502394773784758 1103 34-35 1.0816415291075359 1103 36-37 1.0865381887078698 1103 38-39 1.0432805681202524 1103 40-41 1.202174537627215 1103 42-43 1.2509810116003734 1103 44-45 1.139648539677232 1103 46-47 1.0661316366817815 1103 48-49 1.1180778167637726 1103 50-51 1.0612719204236853 1103 52-53 0.9600091649941618 1103 54-55 0.9908795957803065 1103 56-57 1.060736657557964 1103 58-59 1.030718958937289 1103 60-61 1.0931963493768215 1103 62-63 1.1097965179226144 1103 64-65 0.9807046477381398 1103 66-67 0.9855406241829847 1103 68-69 0.9213159139190239 1103 70-71 1.0841050289012593 1103 72-73 1.0769941407981243 1103 74-75 0.9867558966792309 1103 76-77 0.8614637484270595 1103 78-79 0.9323817112138357 1103 80-81 0.9516641451840044 1103 82-83 0.9285353767474653 1103 84-85 0.8607228937159519 1103 86-87 0.8986638676325747 1103 88-89 0.8642013238721731 1103 90-91 0.8935221609492494 1103 92-93 0.9774634069212134 1103 94-95 0.8628133203074775 1103 96-97 0.9011129151485449 1103 98-99 0.9516221090282251 1103 100-101 0.9404380858902925 1103 102-103 0.9215917247331333 1103 104-105 0.9462213653603477 1103 106-107 0.9599050594106906 1103 108-109 0.8645073274886457 1103 110-111 0.9326554875574935 1103 112-113 0.8215233218356595 1103 114-115 0.8659026084728119 1103 116-117 0.9213483422849045 1103 118-119 0.9703494161511266 1103 120-121 0.8635235608088436 1103 122-123 0.9103217253574414 1103 124-125 0.978888986106579 1104 1 0.2854112976050587 1104 2 -0.6301565910010538 1104 3 0.0928341785206328 1104 4 0.35281504967394994 1104 5 0.6583214310056533 1104 6 0.54029464296665 1104 7 0.7221175224995449 1104 8 0.7108385671195521 1104 9 0.7164784355931815 1104 10-11 0.8924901751586845 1104 12-13 0.7445021113074048 1104 14-15 0.7002898351396283 1104 16-17 0.8332626188281935 1104 18-19 0.7993473700916596 1104 20-21 0.8107238639775431 1104 22-23 0.7894886821975007 1104 24-25 0.673887510417309 1104 26-27 0.6074760697126891 1104 28-29 0.9123929361338412 1104 30-31 0.7700439575729661 1104 32-33 0.8894737747581658 1104 34-35 0.6549037671306905 1104 36-37 0.7564838744848998 1104 38-39 0.8765303735697927 1104 40-41 0.912691455521589 1104 42-43 0.9436996943072415 1104 44-45 0.9094629705238688 1104 46-47 0.9011556490050836 1104 48-49 1.0234739268206994 1104 50-51 0.8693991990625776 1104 52-53 0.7216833915894654 1104 54-55 0.8036536987161611 1104 56-57 0.9228476899013138 1104 58-59 0.9651130808484716 1104 60-61 0.9411495719851821 1104 62-63 0.8474184450914528 1104 64-65 0.857413760161652 1104 66-67 0.788665557556115 1104 68-69 0.8824078883129829 1104 70-71 0.899348590080713 1104 72-73 0.9306102474437559 1104 74-75 0.826111588212818 1104 76-77 0.8922027869746998 1104 78-79 0.9295359444185323 1104 80-81 0.8141197716897679 1104 82-83 0.8213404078755353 1104 84-85 0.8793131891128354 1104 86-87 0.8816309086534098 1104 88-89 0.8415512535142007 1104 90-91 0.816140983306024 1104 92-93 0.873194090459581 1104 94-95 0.8363255423401839 1104 96-97 0.7594979065137011 1104 98-99 0.7459359722139531 1104 100-101 0.9166916704158048 1104 102-103 0.9261400745188588 1104 104-105 0.9351291910857817 1104 106-107 0.8982644793838226 1104 108-109 0.8080898941688126 1104 110-111 0.7967607953203206 1104 112-113 0.7949731811197118 1104 114-115 0.8710740527301084 1104 116-117 0.8798677200432579 1104 118-119 0.9045632457569148 1104 120-121 0.9281378978448487 1104 122-123 0.9819540036765275 1104 124-125 0.7722884621528898 1105 1 0.41248320134037897 1105 2 0.17594493538915756 1105 3 0.3799770886505627 1105 4 0.43334548930582883 1105 5 0.5393071132481282 1105 6 0.5523015692135687 1105 7 0.5947825983765682 1105 8 0.7378235999450453 1105 9 0.6930209473532898 1105 10-11 0.7426514557204911 1105 12-13 0.7543826198828967 1105 14-15 0.8526091582883524 1105 16-17 0.8523096677225226 1105 18-19 0.9071904142045142 1105 20-21 1.0765698662563565 1105 22-23 0.9407872233518617 1105 24-25 1.0687302415345954 1105 26-27 0.8969041728099114 1105 28-29 1.0827879157110374 1105 30-31 0.9282116125724684 1105 32-33 1.061578451926998 1105 34-35 1.0214563194761084 1105 36-37 0.9145334891619896 1105 38-39 0.9058396424681234 1105 40-41 0.9421602805778946 1105 42-43 0.8975464488165557 1105 44-45 0.9052487878554913 1105 46-47 0.9918834320139212 1105 48-49 0.9678012564089258 1105 50-51 0.9234042733648593 1105 52-53 0.9041499137532654 1105 54-55 0.9362678363548227 1105 56-57 0.8295962256221792 1105 58-59 0.798219486976155 1105 60-61 0.9940359311700426 1105 62-63 0.965630687317482 1105 64-65 1.0616384844685207 1105 66-67 0.907755879225757 1105 68-69 0.9409764509345457 1105 70-71 0.9110092690533342 1105 72-73 0.9461903336379187 1105 74-75 0.8813593394926187 1105 76-77 0.8518940340960803 1105 78-79 0.9000947748953223 1105 80-81 0.7715415081579167 1105 82-83 0.8183313557315195 1105 84-85 0.5842361342904603 1105 86-87 0.6545023273432129 1105 88-89 0.6777910640770521 1105 90-91 0.7478042261092419 1105 92-93 0.6749624828532035 1105 94-95 0.663064798816297 1105 96-97 0.6751908272828793 1105 98-99 0.8050104684114778 1105 100-101 0.8267239265281674 1105 102-103 0.4299393591190217 1105 104-105 0.34873285020563216 1105 106-107 0.6385607384686658 1105 108-109 0.7301831512292729 1105 110-111 0.6284463577655366 1105 112-113 0.3861242564644449 1105 114-115 0.5666015999185845 1105 116-117 0.4043854238141087 1105 118-119 0.39661868956965307 1105 120-121 0.3243080945505241 1105 122-123 0.4224303165497503 1105 124-125 0.20377776792092206 1106 1 0.5865823373568766 1106 2 0.5561574569469627 1106 3 0.7810321757142056 1106 4 0.5006492963998639 1106 5 0.7158902741511568 1106 6 0.47916566456351006 1106 7 0.5726319408916112 1106 8 0.7236620097680131 1106 9 0.578644984362974 1106 10-11 0.7532378087536244 1106 12-13 0.723372162217828 1106 14-15 0.82239615463277 1106 16-17 1.03228441865474 1106 18-19 0.9474877464039579 1106 20-21 0.8780895027417799 1106 22-23 0.9075649010675662 1106 24-25 0.9857063185571064 1106 26-27 0.8792399172749512 1106 28-29 1.160123534830646 1106 30-31 1.0665117100847823 1106 32-33 0.9013798019293446 1106 34-35 0.9227417849993671 1106 36-37 0.870446225606841 1106 38-39 1.0088569960598477 1106 40-41 0.9843321375092771 1106 42-43 0.906832102036546 1106 44-45 0.9762777966431742 1106 46-47 0.8787233839069941 1106 48-49 0.8781567167448117 1106 50-51 0.9769309471922796 1106 52-53 1.0568035406391516 1106 54-55 0.9800869892640236 1106 56-57 0.9164032663067267 1106 58-59 0.8326231416630101 1106 60-61 0.9988185982763831 1106 62-63 0.9689932229203997 1106 64-65 0.9390882643426757 1106 66-67 0.8641242944932124 1106 68-69 0.9244340690758222 1106 70-71 0.910821636596971 1106 72-73 0.9124092278855933 1106 74-75 1.034261214595869 1106 76-77 1.0746942379913058 1106 78-79 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-3.5035695846920696 2216 74-75 -3.350695083712928 2216 76-77 -3.517445565298434 2216 78-79 -3.0010496613351876 2216 80-81 -2.6479191881493307 2216 82-83 -2.6865258977623405 2216 84-85 -2.630784459225225 2216 86-87 -2.8161277990340885 2216 88-89 -2.5469996565199864 2216 90-91 -2.6593577410115365 2216 92-93 -2.7219238479807473 2216 94-95 -2.3738778561631086 2216 96-97 -2.530921398576943 2216 98-99 -2.546306812540397 2216 100-101 -2.804280051364607 2216 102-103 -2.597697490953145 2216 104-105 -2.519293340522001 2216 106-107 -2.6491268033344078 2216 108-109 -2.6836176725113567 2216 110-111 -2.546241571266041 2216 112-113 -2.5840894232623803 2216 114-115 -2.6650532738801296 2216 116-117 -2.8303795988915645 2216 118-119 -2.88992019169201 2216 120-121 -2.8250793803676295 2216 122-123 -2.7617616079758918 2216 124-125 -2.794088955069892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65392.0 3 19777.0 4 9183.0 5 7194.0 6 7695.0 7 9267.0 8 10741.0 9 11467.0 10 12098.0 11 13355.0 12 15151.0 13 16546.0 14 18067.0 15 15172.0 16 10593.0 17 8940.0 18 8522.0 19 7832.0 20 7736.0 21 8097.0 22 9694.0 23 12764.0 24 17448.0 25 23150.0 26 27940.0 27 31942.0 28 36813.0 29 43597.0 30 50177.0 31 59475.0 32 72131.0 33 89334.0 34 114787.0 35 154893.0 36 244619.0 37 383128.0 38 153612.0 39 632.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99699207196902 20.07213938466355 13.38566692977279 24.54520161359464 2 15.856800272171313 20.56707888819125 39.123243264174896 24.45287757546254 3 19.540222260181398 25.207563899940354 28.616426777581168 26.635787062297084 4 13.083072064785387 17.181307195974355 36.39135936859901 33.34426137064125 5 13.14958531166049 38.59587711643679 34.07373367707286 14.180803894829861 6 31.93138366473056 37.6294184991683 16.77331191399243 13.665885922108705 7 28.59991376260393 32.55992393419424 20.335607199716964 18.504555103484876 8 25.879220171136915 37.20378714632322 18.601617171403916 18.31537551113595 9 26.022499239890546 16.620359877276876 19.44592166726554 37.911219215567044 10-11 24.870606755718498 27.57518415409149 26.736098627051526 20.81811046313848 12-13 25.791211409698377 24.355462281972848 27.771831464022373 22.081494844306402 14-15 22.72469445600813 25.763604288534587 25.684409143404302 25.827292112052973 16-17 22.043421857166994 29.137754759178137 26.3108014268754 22.50802195677947 18-19 21.991853323334947 27.677583537709683 28.7582053430192 21.57235779593617 20-21 22.66986260169973 26.768425370807343 28.999820871361308 21.56189115613162 22-23 22.342563789554113 26.495647109134783 28.902653531559668 22.259135569751436 24-25 22.154208963045637 26.686175102724714 29.101014339170383 22.05860159505926 26-27 22.032094666496402 26.90641202325534 28.993632256306245 22.067861053942014 28-29 21.955106001638814 26.720775745990977 29.181430790516337 22.142687461853875 30-31 22.090553638248696 26.78402132494841 29.053473236847005 22.071951799955887 32-33 22.01972257050172 26.73086618769943 29.02295991137116 22.226451330427686 34-35 22.238815541075788 26.667462703728827 29.060963724480516 22.03275803071487 36-37 22.337220740861948 26.55080086049419 28.932113221930493 22.179865176713367 38-39 22.123583648293135 26.770201237063706 28.97644006697767 22.129775047665483 40-41 22.34608706323685 26.67887809632159 28.877930480535515 22.097104359906044 42-43 22.154353901138542 26.629240503180117 28.766291022972588 22.450114572708753 44-45 22.28613552199301 26.81315407020936 28.59320902993486 22.307501377862764 46-47 22.385231739443732 27.124196465318068 28.075986181013903 22.414585614224304 48-49 22.419739082052416 27.078328729858242 27.976662827443064 22.52526936064627 50-51 22.511894975944703 27.23389776539741 27.830595199333096 22.423612059324793 52-53 22.541586026453857 27.256861811835638 27.68583733977681 22.515714821933695 54-55 22.565854100779397 27.18726384924827 27.726744799915533 22.520137250056802 56-57 22.352851426003227 27.366984035375474 27.781951499692916 22.498213038928387 58-59 22.457759580709475 27.15454538670137 27.753729282646027 22.63396574994313 60-61 22.56709790868902 27.165013507753898 27.78412580481282 22.48376277874426 62-63 22.414358297387285 27.208961387227255 27.955439614231597 22.421240701153867 64-65 22.374538649003416 27.081099652368067 27.94673826607483 22.597623432553686 66-67 22.282376458088372 27.224191124076196 27.825696629170004 22.66773578866543 68-69 22.327998571549397 27.048491586016883 27.957885030733248 22.665624811700468 70-71 22.327955107932123 26.95610905928873 27.89930241724393 22.816633415535218 72-73 22.468806913683164 27.15009858013101 27.574823742927123 22.806270763258706 74-75 22.341287698283267 27.341061041449453 27.583749700094312 22.733901560172967 76-77 22.49187168161039 27.135108779744755 27.751431974343177 22.62158756430168 78-79 22.521044637711604 27.132497951162343 27.664573834857464 22.681883576268586 80-81 22.34200721916287 27.267721817610415 27.69377349275306 22.696497470473656 82-83 22.50875296475845 27.346011530944462 27.519288032312517 22.62594747198457 84-85 22.45834623208916 27.182331284273836 27.616420484698388 22.742901998938617 86-87 22.377502345279286 27.09954321643514 27.752434695649914 22.770519742635656 88-89 22.508694217288266 27.168053927088533 27.62762712960644 22.695624726016757 90-91 22.368365044906994 27.250974454396747 27.75137219652607 22.629288304170185 92-93 22.2986089784645 27.248492896455513 27.72484514336591 22.72805298171408 94-95 22.272096670002995 27.164445535361608 27.724240833600767 22.83921696103463 96-97 22.368289391559706 27.139359302017667 27.5624408816328 22.929910424789828 98-99 22.269124736795263 27.11239595500823 27.546542115798477 23.071937192398035 100-101 22.14795894534661 27.192640832038617 27.590771354392828 23.06862886822195 102-103 22.185681879555624 26.983080605176625 27.73964812368657 23.091589391581184 104-105 22.134737825515526 26.89278216873531 27.80482681323359 23.167653192515576 106-107 22.06583631860599 27.041650081818585 27.71999800981823 23.172515589757197 108-109 22.07994922840611 27.017298773194874 27.50292928988595 23.39982270851306 110-111 22.02057709056645 27.03657114458719 27.6255304517225 23.31732131312386 112-113 22.185165038066998 26.96606697049504 27.660350176569835 23.18841781486813 114-115 22.041878840093723 27.09606339345363 27.547953751237248 23.3141040152154 116-117 21.82321442243667 27.05832144556054 27.712989684794586 23.4054744472082 118-119 21.77633235568329 26.99870282396301 27.63988314635811 23.585081673995585 120-121 21.806676612781285 27.061752434628072 27.62910667691686 23.502464275673788 122-123 21.772333624846183 27.11351421871424 27.553381678030508 23.56077047840907 124-125 21.734663472887057 26.97183920023572 27.56359688280329 23.729900444073937 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 134.0 1 110.0 2 135.5 3 251.0 4 238.5 5 211.5 6 501.0 7 1103.5 8 1758.0 9 2061.5 10 1733.0 11 1279.5 12 1191.5 13 1257.5 14 1399.5 15 1527.5 16 1664.5 17 1875.5 18 2089.0 19 2248.0 20 2464.5 21 2815.0 22 3311.5 23 4107.5 24 5059.5 25 6410.0 26 8414.0 27 10887.5 28 13437.0 29 16393.0 30 20085.5 31 23740.0 32 27337.0 33 31356.0 34 35660.5 35 39849.5 36 44733.5 37 49074.5 38 52211.0 39 55218.5 40 57175.0 41 58767.5 42 60873.0 43 63608.0 44 66987.5 45 69205.5 46 69991.5 47 70507.0 48 71523.0 49 71123.0 50 68804.0 51 65371.5 52 62298.0 53 60472.0 54 58623.5 55 55165.0 56 49957.0 57 45384.5 58 41663.0 59 37056.0 60 30695.5 61 24446.0 62 19236.5 63 14671.0 64 10772.0 65 7907.0 66 5722.0 67 4102.5 68 3094.5 69 2274.0 70 1587.0 71 1139.0 72 905.0 73 796.0 74 595.0 75 408.5 76 296.5 77 179.5 78 110.0 79 63.0 80 39.5 81 26.0 82 18.5 83 14.0 84 10.5 85 10.5 86 6.0 87 3.5 88 3.0 89 1.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05926053684960594 2 0.07053772856352349 3 0.0 4 0.00873429554313222 5 0.033997416196369076 6 2.21121406155246E-4 7 5.52803515388115E-5 8 0.0 9 6.080838669269266E-4 10-11 0.06080838669269265 12-13 0.010171584683141317 14-15 0.007628688512355987 16-17 0.05276509554379558 18-19 1.3820087884702876E-4 20-21 0.011332472065456359 22-23 0.012189317514307936 24-25 0.0 26-27 0.0 28-29 0.05232285273148509 30-31 0.0 32-33 0.0038143442561779936 34-35 0.0 36-37 2.764017576940575E-5 38-39 0.0 40-41 0.0 42-43 0.0036485032015615595 44-45 0.0 46-47 2.764017576940575E-5 48-49 8.292052730821726E-5 50-51 2.21121406155246E-4 52-53 0.0 54-55 0.0 56-57 0.0013267284369314761 58-59 2.764017576940575E-5 60-61 0.0 62-63 0.0 64-65 4.146026365410863E-4 66-67 0.0 68-69 4.42242812310492E-4 70-71 0.0 72-73 0.001464929315778505 74-75 0.003261540740789879 76-77 4.146026365410863E-4 78-79 4.698829880798978E-4 80-81 0.0014096489642396934 82-83 1.3820087884702876E-4 84-85 5.52803515388115E-5 86-87 4.42242812310492E-4 88-89 0.0 90-91 0.0 92-93 0.0061084788450386715 94-95 0.004726470056568384 96-97 0.0049752316384930355 98-99 4.42242812310492E-4 100-101 3.593222850022748E-4 102-103 8.292052730821726E-5 104-105 1.9348123038584025E-4 106-107 0.004477708474643732 108-109 0.0041736665411802685 110-111 4.146026365410863E-4 112-113 0.0024876158192465177 114-115 2.487615819246518E-4 116-117 0.0016307703703949395 118-119 0.0017413310734725623 120-121 0.011857635405075069 122-123 3.869624607716805E-4 124-125 0.001547849843086722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1808961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.681576023339076 #Duplication Level Percentage of deduplicated Percentage of total 1 79.60985333746433 48.30851367504216 2 11.510236224819812 13.969185490459896 3 3.7743252629904114 6.870960161488856 4 1.6494937662559321 4.00375525508333 5 0.9038268491129977 2.7422818828192694 6 0.5473151049994603 1.9927165891647936 7 0.3815289103089701 1.620624290321084 8 0.25414328260681224 1.2337451939460982 9 0.19830625910876643 1.0830182704211326 >10 1.0756600630988864 11.786235633053964 >50 0.07003447597197955 2.949713333286355 >100 0.024544022957976735 2.4977250687156545 >500 1.8311007590956132E-4 0.06355725371392788 >1k 4.5777518977390335E-4 0.5942936564492016 >5k 9.155503795478066E-5 0.283674246034367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5106 0.2822614749571716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4434 0.2451130787230902 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2728 0.1508047989978778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.528035153881151E-5 0.0 0.0 0.0 0.0 7 5.528035153881151E-5 0.0 0.0 0.0 0.0 8 5.528035153881151E-5 0.0 0.0 0.0 0.0 9 5.528035153881151E-5 0.0 0.0 0.0 0.0 10-11 5.528035153881151E-5 0.0 0.0 0.0 0.0 12-13 1.1056070307762302E-4 0.0 0.0 0.0 0.0 14-15 1.1056070307762302E-4 0.0 0.0 5.528035153881151E-5 0.0 16-17 1.1056070307762302E-4 0.0 0.0 5.528035153881151E-5 0.0 18-19 1.3820087884702876E-4 0.0 0.0 8.292052730821726E-5 0.0 20-21 1.6584105461643452E-4 0.0 0.0 1.6584105461643452E-4 0.0 22-23 2.2112140615524603E-4 0.0 0.0 2.2112140615524603E-4 0.0 24-25 2.764017576940575E-4 0.0 0.0 3.3168210923286903E-4 0.0 26-27 2.764017576940575E-4 0.0 0.0 5.804436911575208E-4 0.0 28-29 5.251633396187093E-4 0.0 0.0 9.397659761597955E-4 0.0 30-31 6.080838669269266E-4 0.0 0.0 0.002321774764630083 0.0 32-33 6.357240426963323E-4 0.0 0.0 0.005223993220417687 0.0 34-35 6.633642184657381E-4 0.0 0.0 0.00859609466428519 0.0 36-37 6.633642184657381E-4 0.0 0.0 0.014068849466627528 0.0 38-39 6.633642184657381E-4 0.0 0.0 0.02484851801669577 0.0 40-41 6.633642184657381E-4 0.0 0.0 0.03814344256177994 0.0 42-43 7.186445700045496E-4 0.0 0.0 0.04953119497877511 0.0 44-45 8.292052730821726E-4 0.0 0.0 0.06384880602732729 0.0 46-47 9.121258003903899E-4 0.0 0.0 0.07954842586434976 0.0 48-49 9.397659761597956E-4 0.0 0.0 0.09792914275100459 0.0 50-51 9.397659761597956E-4 0.0 0.0 0.11979252178460453 0.0 52-53 9.397659761597956E-4 0.0 0.0 0.14436463804360625 0.0 54-55 9.397659761597956E-4 0.0 0.0 0.16951719799376547 0.0 56-57 9.95046327698607E-4 0.0 0.0 0.20008723239472825 0.0 58-59 9.95046327698607E-4 0.0 0.0 0.2349691342157183 0.0 60-61 9.95046327698607E-4 0.0 0.0 0.2768992808579068 0.0 62-63 0.0010503266792374186 0.0 0.0 0.32081952015549253 0.0 64-65 0.0010503266792374186 0.0 0.0 0.3635788720707633 0.0 66-67 0.0010503266792374186 0.0 0.0 0.4136628705649265 0.0 68-69 0.0011608873823150416 0.0 0.0 0.47394609391800047 0.0 70-71 0.0012714480853926646 0.0 0.0 0.5445391028330627 0.0 72-73 0.0013820087884702876 0.0 0.0 0.6173156856339082 0.0 74-75 0.0013820087884702876 0.0 0.0 0.6927457253086164 0.0 76-77 0.0013820087884702876 0.0 0.0 0.7718519083606556 0.0 78-79 0.0014096489642396934 0.0 0.0 0.8698916118147378 0.0 80-81 0.0015478498430867222 0.0 0.0 0.9831334119419932 0.0 82-83 0.0015478498430867222 0.0 0.0 1.1005212384346594 0.0 84-85 0.001575490018856128 0.0 0.0 1.2241557446512115 0.0 86-87 0.0016031301946255337 0.0 0.0 1.3567456678170509 0.0 88-89 0.0016031301946255337 0.0 0.0 1.5111713298407206 0.0 90-91 0.0016031301946255337 0.0 0.0 1.683341984708349 0.0 92-93 0.0016584105461643452 0.0 0.0 1.8796148728468993 0.0 94-95 0.0016584105461643452 0.0 0.0 2.0754455181731393 0.0 96-97 0.0016584105461643452 0.0 0.0 2.270419318050527 0.0 98-99 0.0018242516007807798 0.0 0.0 2.4892189494411436 0.0 100-101 0.001990092655397214 0.0 0.0 2.7511648952078014 0.0 102-103 0.002100653358474837 0.0 0.0 3.0441783985392723 0.0 104-105 0.002432335467707706 0.0 0.0 3.3318849881230164 0.0 106-107 0.0025981765223241408 0.0 0.0 3.607623381598608 0.0 108-109 0.0026534568738629523 0.0 0.0 3.9128814827959255 0.0 110-111 0.0026534568738629523 0.0 0.0 4.2708217590097295 0.0 112-113 0.0026534568738629523 0.0 0.0 4.661709124740666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4720 0.0 43.171173 1 GTATCAA 6760 0.0 42.129955 1 ATCAACG 8500 0.0 33.32123 3 TCAACGC 8580 0.0 32.807026 4 GGACCGA 385 0.0 32.48359 6 CAACGCA 8715 0.0 31.922665 5 TATCAAC 8865 0.0 31.701847 2 AACGCAG 8960 0.0 31.305332 6 GTACATG 7760 0.0 29.867414 1 TACATGG 7650 0.0 29.498442 2 AGGACCG 610 0.0 27.344997 5 ACATGGG 8090 0.0 27.213612 3 ACGCAGA 10575 0.0 26.244303 7 CGCAGAG 10595 0.0 26.026123 8 GCAGAGT 11375 0.0 23.09025 9 GAGTACT 5925 0.0 23.070652 12-13 CTATACG 130 0.002050523 22.888624 4 CATGGGA 5370 0.0 22.828955 4 GTACCTG 1835 0.0 22.403856 1 AGTACTT 6335 0.0 22.04762 12-13 >>END_MODULE