FastQCFastQC Report
Thu 26 May 2016
SRR1364609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186576
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125480.5738652578277637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.13756667959403196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82200.047.330381
GGTATCA49600.039.7598651
ATCAACG97600.038.5175133
CAACGCA99700.037.526945
TCAACGC99400.037.5203254
GTACATG137500.037.394411
TATCAAC106300.036.317872
AACGCAG100950.036.31746
TACATGG136350.036.2661672
ACGCATA3200.033.471367
ACATGGG142800.032.7923853
CGCATAG3150.032.109948
ACGCAGA119850.030.1371027
AACGCAT3750.028.5681216
CGCAGAG119400.028.4533148
CATGGGA92750.028.0702444
ATGGGAT33000.026.1777765
GTATAGG5700.026.1314661
GTATAGA7200.025.6523881
GCAGAGT126000.025.1207289
Produced by FastQC (version 0.11.5)