##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186576 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.424049289848604 31.0 26.0 31.0 10.0 34.0 2 26.186648440301184 31.0 26.0 31.0 10.0 34.0 3 26.849638887466067 31.0 26.0 31.0 10.0 34.0 4 30.12210414822078 35.0 30.0 37.0 7.0 37.0 5 30.581869095791777 35.0 31.0 37.0 10.0 37.0 6 30.490497014510357 35.0 31.0 37.0 8.0 37.0 7 30.39357653244159 35.0 31.0 37.0 8.0 37.0 8 29.970092052597302 35.0 30.0 37.0 8.0 37.0 9 30.96995027842618 37.0 30.0 39.0 7.0 39.0 10-11 30.987184758270466 37.0 29.5 39.0 7.0 39.0 12-13 30.956519462392343 37.0 30.0 39.0 4.5 39.0 14-15 31.5946077337353 38.0 29.5 40.0 2.0 41.0 16-17 31.99681282516592 38.0 30.5 40.0 2.0 41.0 18-19 31.8246431864248 38.0 30.0 40.0 2.0 41.0 20-21 31.74014006373435 38.0 30.0 40.0 2.0 41.0 22-23 31.812156083301012 38.0 30.0 40.0 2.0 41.0 24-25 31.913343967920618 38.0 30.0 40.0 2.0 41.0 26-27 31.72972537885717 38.0 30.0 40.0 2.0 41.0 28-29 31.622080595414932 38.0 30.0 40.0 2.0 41.0 30-31 31.474576689765186 38.0 30.0 40.0 2.0 41.0 32-33 31.35348462619182 37.0 30.0 40.0 2.0 41.0 34-35 31.379196744133296 37.5 30.0 40.0 2.0 41.0 36-37 31.298338132312804 37.0 30.0 40.0 2.0 41.0 38-39 30.991734794491478 37.0 29.0 40.0 2.0 41.0 40-41 30.838313189205408 37.0 29.0 40.0 2.0 41.0 42-43 30.328545634819005 36.5 27.0 40.0 2.0 41.0 44-45 30.20761775488252 36.0 27.0 40.0 2.0 41.0 46-47 30.14556548686165 36.0 27.5 40.0 2.0 41.0 48-49 30.057859639911896 36.0 27.0 40.0 2.0 41.0 50-51 29.811378154703974 36.0 27.0 40.0 2.0 41.0 52-53 29.69418625284463 36.0 27.0 40.0 2.0 41.0 54-55 29.50937012937122 35.5 26.5 40.0 2.0 41.0 56-57 29.280365054770563 35.0 26.0 40.0 2.0 41.0 58-59 29.062694139147233 35.0 26.0 39.0 2.0 41.0 60-61 28.748559620154982 35.0 26.0 39.0 2.0 41.0 62-63 28.559203293185327 34.5 26.0 39.0 2.0 40.0 64-65 28.31404030776886 34.0 25.5 38.5 2.0 40.0 66-67 28.035165711139243 34.0 25.0 38.0 2.0 40.0 68-69 27.777833242475907 34.0 25.0 37.5 2.0 40.0 70-71 27.340783947139272 34.0 23.5 37.0 2.0 39.5 72-73 27.111257509457708 33.0 23.0 36.5 2.0 39.0 74-75 27.067258810121395 33.5 24.0 36.0 2.0 39.0 76-77 26.82140913464705 33.0 23.5 36.0 2.0 39.0 78-79 26.541969728013115 33.0 23.0 35.0 2.0 37.5 80-81 26.1493959048302 33.0 21.0 35.0 2.0 37.0 82-83 26.27707726600859 33.0 23.0 35.0 2.0 37.0 84-85 26.134366242014913 33.0 23.0 35.0 2.0 36.0 86-87 26.01772497274277 33.0 23.0 35.0 2.0 36.0 88-89 25.865941545137236 33.0 22.5 35.0 2.0 36.0 90-91 25.750635468421862 33.0 21.0 35.0 2.0 35.0 92-93 25.634987990355697 33.0 20.0 35.0 2.0 35.0 94-95 25.53765704919472 33.0 20.0 35.0 2.0 35.0 96-97 25.435240988650747 33.0 20.0 35.0 2.0 35.0 98-99 25.352057737759857 33.0 19.0 35.0 2.0 35.0 100-101 25.25244811980009 33.0 18.5 35.0 2.0 35.0 102-103 24.14892279070108 31.0 16.0 34.0 2.0 35.0 104-105 24.96065217948061 32.5 18.0 35.0 2.0 35.0 106-107 25.11341659288312 33.0 18.0 35.0 2.0 35.0 108-109 25.0726741261223 33.0 17.0 35.0 2.0 35.0 110-111 25.003951383350042 33.0 15.0 35.0 2.0 35.0 112-113 24.915243055809633 33.0 9.0 35.0 2.0 35.0 114-115 24.813761104118953 33.0 6.0 35.0 2.0 35.0 116-117 24.70517283643468 33.0 2.0 35.0 2.0 35.0 118-119 24.577260291890152 33.0 2.0 35.0 2.0 35.0 120-121 24.397249398145778 33.0 2.0 35.0 2.0 35.0 122-123 24.1912954317618 33.0 2.0 35.0 2.0 35.0 124-125 23.269255905122897 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8184705303818944 1101 2 2.1676352844763542 1101 3 1.712636446316985 1101 4 1.871647061664298 1101 5 1.7039546258599039 1101 6 1.6054477987075764 1101 7 1.7551347588942825 1101 8 1.8470973148497194 1101 9 2.251299328923622 1101 10-11 2.1511141321778133 1101 12-13 2.261969022323303 1101 14-15 2.5499666927667377 1101 16-17 2.1349942700546976 1101 18-19 2.240411693273117 1101 20-21 2.359252551720722 1101 22-23 2.103967972012878 1101 24-25 2.1357777114648187 1101 26-27 2.3312804013807664 1101 28-29 2.3459565426474107 1101 30-31 2.331647734247806 1101 32-33 2.3639783639885934 1101 34-35 2.046561132134478 1101 36-37 1.8715353312566272 1101 38-39 1.9814541266070584 1101 40-41 1.9567747043760164 1101 42-43 2.0326261469660345 1101 44-45 1.9221616948527291 1101 46-47 1.5939436513709744 1101 48-49 1.487937829871207 1101 50-51 1.567575156689795 1101 52-53 1.5017138519311928 1101 54-55 1.4596721716884495 1101 56-57 1.4204650912622725 1101 58-59 1.3921606729675915 1101 60-61 1.5306835803667838 1101 62-63 1.2211430938066137 1101 64-65 1.2352594984483218 1101 66-67 1.2638878433456782 1101 68-69 1.0241213620118934 1101 70-71 1.1102714562988645 1101 72-73 1.0232613940861128 1101 74-75 1.1441640458142324 1101 76-77 1.1873976459216138 1101 78-79 1.2469034278155213 1101 80-81 1.2973348039516175 1101 82-83 1.2905754966209635 1101 84-85 1.2567237233243347 1101 86-87 1.2284065912112148 1101 88-89 1.2524333169228825 1101 90-91 1.2598119754250838 1101 92-93 1.2236222585199172 1101 94-95 1.1321421623533858 1101 96-97 1.1618362457514202 1101 98-99 1.2513747743857309 1101 100-101 1.1833506204736004 1101 102-103 1.0060557323949162 1101 104-105 1.1675062237912677 1101 106-107 1.1947033730183172 1101 108-109 1.2133100742523624 1101 110-111 1.300223422066697 1101 112-113 1.1411787611879625 1101 114-115 1.1780461601112258 1101 116-117 1.0776478594751708 1101 118-119 1.0723437593036245 1101 120-121 1.1803260399233544 1101 122-123 1.1315593689998984 1101 124-125 1.0049318417940754 1102 1 0.9091519448889436 1102 2 1.3568461472653084 1102 3 1.0567561369609173 1102 4 0.9722333239272913 1102 5 0.9018756039100957 1102 6 0.8252276961854754 1102 7 0.7910141218348095 1102 8 0.6319838082348852 1102 9 1.3127875720530895 1102 10-11 1.3169665691275618 1102 12-13 1.3492863512387778 1102 14-15 1.514808760843998 1102 16-17 1.2488040658304058 1102 18-19 1.1772027542377401 1102 20-21 1.6162294380979176 1102 22-23 1.5111625573915042 1102 24-25 1.2026504031128056 1102 26-27 1.5797919286752418 1102 28-29 1.239920051300313 1102 30-31 1.258515862486174 1102 32-33 1.4358732893740296 1102 34-35 1.3034797343068263 1102 36-37 1.2716817914048058 1102 38-39 1.4479305418848263 1102 40-41 1.4313463927766605 1102 42-43 1.7054983166798898 1102 44-45 1.3068252183578046 1102 46-47 1.0917972090266552 1102 48-49 1.0350490369773837 1102 50-51 1.0849368687461443 1102 52-53 0.9469797829862259 1102 54-55 0.9565876535006375 1102 56-57 1.0101441954846422 1102 58-59 0.9117814601885428 1102 60-61 0.8356963968010547 1102 62-63 0.6740789732164068 1102 64-65 0.676375678452807 1102 66-67 0.6928263258667435 1102 68-69 0.6560478939283527 1102 70-71 0.8251310236932561 1102 72-73 0.3978511206048161 1102 74-75 0.5236630452843336 1102 76-77 0.3212975948058059 1102 78-79 0.4820395734398275 1102 80-81 0.6100022480533625 1102 82-83 0.5999670779267241 1102 84-85 0.5467013701136203 1102 86-87 0.6474433650774039 1102 88-89 0.4837166142346412 1102 90-91 0.5453246491942387 1102 92-93 0.6097361404213153 1102 94-95 0.6006633974350422 1102 96-97 0.41589076959430216 1102 98-99 0.5458193619898424 1102 100-101 0.5959918741649695 1102 102-103 0.4943153538568126 1102 104-105 0.6236608062463169 1102 106-107 0.603330184970698 1102 108-109 0.7204566360699332 1102 110-111 0.6177042325544768 1102 112-113 0.46011733272830924 1102 114-115 0.5786756670052995 1102 116-117 0.6100807237081121 1102 118-119 0.551889236210382 1102 120-121 0.5279166392995656 1102 122-123 0.43892721401638823 1102 124-125 0.4130280988142978 1103 1 1.2033242212690745 1103 2 1.4528421312719573 1103 3 1.0849247800504855 1103 4 0.9988694379956264 1103 5 1.0397473851148078 1103 6 1.0630268974723478 1103 7 0.9755194307623292 1103 8 1.0748481429595316 1103 9 1.6506373072867007 1103 10-11 1.5754359679358423 1103 12-13 1.6023017581277195 1103 14-15 1.8237259266864854 1103 16-17 1.4407501285965267 1103 18-19 1.4016309786161045 1103 20-21 1.6973220593304745 1103 22-23 1.5112823786759826 1103 24-25 1.4653045757900216 1103 26-27 1.653503348822305 1103 28-29 1.5446690970485655 1103 30-31 1.60121804957058 1103 32-33 1.7127129469266151 1103 34-35 1.5186210217819962 1103 36-37 1.4079978046533803 1103 38-39 1.4748656126824606 1103 40-41 1.5077986234042413 1103 42-43 1.6990532581507019 1103 44-45 1.5417088161075831 1103 46-47 1.3527878175384025 1103 48-49 1.2557803619641064 1103 50-51 1.21522602885668 1103 52-53 1.0692745586159802 1103 54-55 1.041378462525742 1103 56-57 1.0237593482245728 1103 58-59 1.1403616757905084 1103 60-61 1.1582530518450724 1103 62-63 1.0953130608687083 1103 64-65 0.9193639859759735 1103 66-67 0.9021188935590914 1103 68-69 1.00914871702566 1103 70-71 1.121620465247812 1103 72-73 0.9839986157864331 1103 74-75 1.0877458159761701 1103 76-77 0.9574316094964388 1103 78-79 0.9171786563178834 1103 80-81 0.976623550283108 1103 82-83 1.0278130481665748 1103 84-85 1.0698867648009234 1103 86-87 1.002837637228712 1103 88-89 0.9869269752684389 1103 90-91 1.0144516048794507 1103 92-93 0.9737305363545481 1103 94-95 1.0818271193103222 1103 96-97 1.0608408822339435 1103 98-99 1.019697272165395 1103 100-101 0.8841417829509588 1103 102-103 0.8409491678909369 1103 104-105 0.9144747940809488 1103 106-107 1.1341785110754685 1103 108-109 1.101139832124943 1103 110-111 1.0687447594436428 1103 112-113 1.0825994036752995 1103 114-115 1.1326806612289673 1103 116-117 1.091091793935032 1103 118-119 1.0740757384452841 1103 120-121 1.1241838078381505 1103 122-123 1.08099350367468 1103 124-125 1.0259624509705318 1104 1 1.2366193189668238 1104 2 1.5555722681561583 1104 3 1.006893426204286 1104 4 1.1518268558104943 1104 5 0.9129580487788758 1104 6 0.8476301086094651 1104 7 0.6891306451320531 1104 8 1.0217293291986422 1104 9 1.335618015244421 1104 10-11 1.512956939330369 1104 12-13 1.5068774246067242 1104 14-15 1.5505613426666116 1104 16-17 1.2667567672529003 1104 18-19 1.2641101947277562 1104 20-21 1.4921885619604822 1104 22-23 1.4227755700921385 1104 24-25 1.2833878125967288 1104 26-27 1.5708404985314601 1104 28-29 1.517601407785687 1104 30-31 1.5629153649039473 1104 32-33 1.6227076358140096 1104 34-35 1.5707778350769317 1104 36-37 1.4774362189511017 1104 38-39 1.575030664999023 1104 40-41 1.5714451592509704 1104 42-43 1.8048855910604296 1104 44-45 1.5765514492006467 1104 46-47 1.33143522294505 1104 48-49 1.3008659421293274 1104 50-51 1.3540789972904825 1104 52-53 1.204777541604951 1104 54-55 1.2456140865439735 1104 56-57 1.3495210569309037 1104 58-59 1.2587179215979845 1104 60-61 1.310281657788174 1104 62-63 1.3088992431631574 1104 64-65 1.2205426498350462 1104 66-67 1.1623265191542416 1104 68-69 1.1215579698932139 1104 70-71 1.2381839175741334 1104 72-73 1.133644018158293 1104 74-75 1.0504545396544067 1104 76-77 0.9719057273150078 1104 78-79 0.9102325835645466 1104 80-81 1.044303953496403 1104 82-83 1.0450326783762556 1104 84-85 1.125453331136157 1104 86-87 1.0008765100728034 1104 88-89 1.0603674746349014 1104 90-91 0.9036183883468425 1104 92-93 0.7861201010730099 1104 94-95 0.8186399736091126 1104 96-97 0.8374769557044921 1104 98-99 1.0322728430162442 1104 100-101 1.0203661959745745 1104 102-103 0.8749996811187906 1104 104-105 0.9277203713960773 1104 106-107 1.0229750635868093 1104 108-109 1.0052791248422501 1104 110-111 0.9231183176968933 1104 112-113 0.9001148717633853 1104 114-115 0.9057269288481002 1104 116-117 0.9511609930914382 1104 118-119 0.9910191720491106 1104 120-121 0.8807353910231583 1104 122-123 0.7416214712529872 1104 124-125 0.5317414852480695 1105 1 1.093754951986984 1105 2 1.3372023417284105 1105 3 0.9555520781599469 1105 4 1.1186359218462663 1105 5 0.9227726667859741 1105 6 0.7427931610074126 1105 7 0.7042574402019319 1105 8 0.8046850681252167 1105 9 1.0663112916433803 1105 10-11 1.0519578092416175 1105 12-13 1.1372756163078286 1105 14-15 1.4124821672398795 1105 16-17 1.0841987007450626 1105 18-19 1.2103146710867705 1105 20-21 1.0718935570127996 1105 22-23 1.173244940634003 1105 24-25 0.8504020631367908 1105 26-27 1.274325281972132 1105 28-29 1.2389624187786161 1105 30-31 1.2126560998144633 1105 32-33 1.3795007871592588 1105 34-35 1.130577685708488 1105 36-37 0.7668452420849086 1105 38-39 1.061494568666614 1105 40-41 1.2221417180944982 1105 42-43 1.3256014684768012 1105 44-45 1.2130147320856501 1105 46-47 0.8512003061267457 1105 48-49 0.8943535340447966 1105 50-51 1.058814317798717 1105 52-53 0.8459289564590904 1105 54-55 0.9039124680051742 1105 56-57 0.9974689989188192 1105 58-59 1.0939209886445909 1105 60-61 0.9608291834983156 1105 62-63 0.8825201157405935 1105 64-65 0.5877478748487981 1105 66-67 0.7331108231241998 1105 68-69 0.6275990907130051 1105 70-71 0.8258357906745672 1105 72-73 0.7741957660962768 1105 74-75 0.7177615639498427 1105 76-77 0.7362630465093609 1105 78-79 0.6968774023091733 1105 80-81 0.7757719846885038 1105 82-83 0.7362141400138391 1105 84-85 0.7764877407071182 1105 86-87 0.7670547160002528 1105 88-89 0.8619832131137883 1105 90-91 0.6877243422499753 1105 92-93 0.7516171408463244 1105 94-95 0.694053394421136 1105 96-97 0.7779416853301093 1105 98-99 0.7529598291204138 1105 100-101 0.7658970371331684 1105 102-103 0.6568455332806415 1105 104-105 0.7008222969186626 1105 106-107 0.7048561145744436 1105 108-109 0.807002888553729 1105 110-111 0.824142146776321 1105 112-113 0.7285518164291851 1105 114-115 0.7502483956458867 1105 116-117 0.6859065750905238 1105 118-119 0.7116931081251785 1105 120-121 0.6923776745279504 1105 122-123 0.8169554772065197 1105 124-125 0.6843345575500273 1106 1 0.4463765842992089 1106 2 -1.848782057265261 1106 3 -0.057377374588430285 1106 4 0.479139209901966 1106 5 0.6697153054967302 1106 6 0.6576971341542546 1106 7 0.658426298831742 1106 8 0.6695385872748716 1106 9 1.1646992942022578 1106 10-11 1.3528716192531256 1106 12-13 1.3064847382484608 1106 14-15 1.4305308472452296 1106 16-17 1.0168924913740653 1106 18-19 1.1531149766963722 1106 20-21 1.3668691557461443 1106 22-23 1.0562443630655842 1106 24-25 1.1632206635921847 1106 26-27 1.2398960336590008 1106 28-29 1.224415996439813 1106 30-31 1.39755541601075 1106 32-33 1.4461085871297001 1106 34-35 1.33669169797993 1106 36-37 1.0624144355356968 1106 38-39 1.378667173722139 1106 40-41 1.3651024199133701 1106 42-43 1.5704165156212113 1106 44-45 1.5103061537518805 1106 46-47 1.2981539545966747 1106 48-49 1.0818153716890286 1106 50-51 1.1363960508324809 1106 52-53 1.0745815883464616 1106 54-55 0.9816699576380223 1106 56-57 1.0498019358120345 1106 58-59 1.00162111654425 1106 60-61 1.1390633779457602 1106 62-63 0.9984452440509024 1106 64-65 1.02452451036406 1106 66-67 0.8343399650688497 1106 68-69 0.757376760168956 1106 70-71 0.8164245775012162 1106 72-73 0.5808639066500589 1106 74-75 0.6541683070721831 1106 76-77 0.614705094555422 1106 78-79 0.7037481579471283 1106 80-81 0.7602528412477696 1106 82-83 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-2.1241055054375764 2216 82-83 -2.2133530981031804 2216 84-85 -2.150402285862235 2216 86-87 -2.2743209741794423 2216 88-89 -2.2985182694752666 2216 90-91 -2.078753732957086 2216 92-93 -2.0739178196269457 2216 94-95 -2.1982288563963905 2216 96-97 -2.2932556094264918 2216 98-99 -2.4018235869288347 2216 100-101 -2.494046335712394 2216 102-103 -2.2905667408249464 2216 104-105 -2.316498287433138 2216 106-107 -2.255125457156389 2216 108-109 -2.373023693230209 2216 110-111 -2.2097765989744644 2216 112-113 -2.4348012833772117 2216 114-115 -2.4530331040373774 2216 116-117 -2.2686498434615423 2216 118-119 -2.2691948543786005 2216 120-121 -2.48688468096778 2216 122-123 -2.3494095342717927 2216 124-125 -2.1884193624966386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 192684.0 3 27525.0 4 18501.0 5 14953.0 6 13846.0 7 14651.0 8 15269.0 9 16149.0 10 17802.0 11 19723.0 12 20930.0 13 21562.0 14 21294.0 15 17178.0 16 12968.0 17 11488.0 18 9983.0 19 9607.0 20 9182.0 21 10050.0 22 12693.0 23 18982.0 24 29177.0 25 36180.0 26 40392.0 27 40738.0 28 47858.0 29 53653.0 30 62170.0 31 72617.0 32 88178.0 33 112156.0 34 147555.0 35 203909.0 36 296739.0 37 337729.0 38 90401.0 39 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.424946103305665 19.396622840465785 13.796499648021587 22.381931408206963 2 13.579731268106986 21.67887056864483 40.85977333798535 23.881624825262833 3 19.10416101853139 27.68313376206407 27.185776745325406 26.02692847407913 4 11.672487851191718 17.885063903075558 35.61835656838306 34.824091677349664 5 13.072332053531447 40.72637627643721 32.05504555164845 14.1462461183829 6 31.008159523263814 37.580105432042174 16.965528306211322 14.446206738482696 7 28.07270930487323 35.14897276128331 19.452172964430066 17.326144969413395 8 25.297851182101894 37.44217805538625 19.068204002511308 18.19176676000055 9 25.908983633001625 19.081816661205654 19.03777722276578 35.97142248302694 10-11 23.790309468445614 29.977594464385408 25.78010606447133 20.45199000269764 12-13 24.80848326811243 27.05599316119657 26.431943185889196 21.703580384801803 14-15 21.995399239987986 27.879066129567683 24.664605236600533 25.460929393843795 16-17 21.868790919095712 31.162471642214935 24.84246183938242 22.12627559930693 18-19 21.969323648089997 29.236898770238014 27.350938409724098 21.44283917194789 20-21 23.19485743870758 28.08425388418241 27.191407377541154 21.529481299568857 22-23 22.891328292754125 27.786129860333475 26.98148862295125 22.34105322396115 24-25 22.487051546826365 27.794462465638425 27.284497552360875 22.43398843517434 26-27 22.30878717850381 27.96284711341428 27.35899121082713 22.369374497254775 28-29 22.24651353822183 27.932239139861398 27.42231239329758 22.398934928619187 30-31 22.27894712613457 27.777084334849416 27.36781304615788 22.576155492858135 32-33 22.159169927537974 27.807253350856005 27.180281677878384 22.853295043727638 34-35 22.58695871236064 27.69056714394335 27.260514286237758 22.46195985745825 36-37 22.688506163571333 27.60706494182246 26.940736000908743 22.76369289369746 38-39 22.543439603796177 27.62230969529162 27.207237711757042 22.627012989155162 40-41 22.614520193311723 27.657228463870226 27.228599062862973 22.49965227995508 42-43 22.38834408733313 27.700699994015576 27.144997057085657 22.76595886156563 44-45 22.504695221216558 27.820814919522107 26.97360207934334 22.70088777991799 46-47 22.671937095648254 28.08358572394422 26.351205886610195 22.89327129379733 48-49 22.627250398294503 27.968336159643947 26.39925279126803 23.005160650793517 50-51 22.775014035038694 28.152123098799116 26.345033130937118 22.72782973522507 52-53 23.07751872452604 28.026433924238663 26.025470349693254 22.87057700154204 54-55 22.858047331910694 28.018098788802266 26.162040646912814 22.96181323237423 56-57 22.706057731027414 28.284735485354677 26.13595913960071 22.873247644017194 58-59 22.83709440546389 27.76373592274453 26.14876610681688 23.250403564974707 60-61 22.864888783431507 27.91808648594671 26.41097475451002 22.806049976111765 62-63 22.797600086290267 28.02390936590623 26.30431013931252 22.87418040849098 64-65 22.667256867380708 27.984330216841514 26.233673683429526 23.11473923234825 66-67 22.732859580399854 28.200328067342646 25.980904868713534 23.08590748354397 68-69 22.839525961042636 27.969131963557732 26.116014797459997 23.07532727793964 70-71 22.626157434439715 28.069688555738892 26.33303886561693 22.97111514420446 72-73 22.94029684687758 28.138871630231186 25.883491927297836 23.037339595593405 74-75 22.699408834268244 28.362196436064796 25.941834859570957 22.996559870096007 76-77 22.838040701630977 28.12115600032607 26.163561241583842 22.87724205645911 78-79 22.984482792575225 28.2312554415106 25.984441115355672 22.799820650558498 80-81 22.699458720418274 28.416731459141808 26.126203909076022 22.757605911363893 82-83 22.80162393199584 28.389472519826796 25.906350689887113 22.902552858290246 84-85 22.827455443526688 28.11691508680011 25.99040973864935 23.065219731023852 86-87 22.702929773081102 28.01536384923564 26.206911716504045 23.074794661179208 88-89 22.78982933841868 28.02238841010177 26.16632260721405 23.02145964426551 90-91 22.67155872033147 28.28167950941444 26.069468780408823 22.97729298984527 92-93 22.716819266513074 28.189069606599276 26.05674057720758 23.037370549680066 94-95 22.72709468561477 28.09549213306796 26.05102207468758 23.12639110662969 96-97 22.740585012197393 28.180225954668437 26.07090497531319 23.00828405782098 98-99 22.544943291422065 28.122727700576693 25.926250793837646 23.406078214163596 100-101 22.382623700665594 28.37829235699651 26.089911330644828 23.149172611693068 102-103 22.628464881035555 28.03165207910042 26.10229087019166 23.237592169672368 104-105 22.408977631481335 28.216957137767473 26.227369768841243 23.146695461909957 106-107 22.359969682328508 28.41933644600867 26.113979789745574 23.10671408191725 108-109 22.39446441269118 28.510976937724642 25.71361831882757 23.38094033075661 110-111 22.32353923656369 28.60817560917087 26.042888944499154 23.025396209766285 112-113 22.434004033818137 28.61623096699458 25.914396976238933 23.03536802294835 114-115 22.092229462441214 28.7910192592267 25.891055155084757 23.22569612324733 116-117 22.133748046018273 28.7892593504951 26.010644678896817 23.066347924589813 118-119 22.1482208032852 28.90423548504871 25.749556216573176 23.197987495092917 120-121 22.265656918693956 28.697158235582375 25.967020911597615 23.070163934126054 122-123 22.31338147640902 28.793304843109162 25.815178943059387 23.078134737422427 124-125 22.277638226618667 28.8557023717185 25.701881788619524 23.16477761304331 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12853.0 1 10035.0 2 5854.0 3 3646.5 4 2357.0 5 1727.0 6 1576.0 7 1723.0 8 2000.5 9 2119.0 10 1952.5 11 1860.5 12 1912.5 13 2004.5 14 2152.5 15 2291.5 16 2457.5 17 2650.0 18 2825.0 19 3165.5 20 3702.0 21 4297.5 22 5069.5 23 6021.5 24 7112.5 25 8656.5 26 11035.5 27 13722.0 28 16564.0 29 20055.5 30 23612.5 31 27251.0 32 30853.0 33 34196.5 34 37681.5 35 41460.0 36 45993.0 37 50610.5 38 53998.0 39 56078.5 40 58351.0 41 61640.5 42 64981.5 43 68696.0 44 73059.5 45 75316.5 46 75715.0 47 77238.0 48 79578.0 49 81337.5 50 82820.5 51 81310.0 52 78111.5 53 76343.0 54 74045.0 55 70152.5 56 64288.0 57 58858.5 58 55759.0 59 51179.5 60 44150.5 61 37395.0 62 31147.0 63 24300.0 64 18508.0 65 14765.0 66 11286.5 67 8699.0 68 6753.5 69 4964.0 70 3781.5 71 2971.5 72 2393.0 73 2038.5 74 1618.5 75 1260.5 76 908.5 77 599.5 78 417.0 79 294.5 80 214.5 81 156.5 82 110.0 83 77.5 84 55.5 85 50.0 86 42.0 87 25.5 88 19.5 89 20.0 90 18.0 91 13.5 92 9.5 93 5.0 94 3.5 95 3.0 96 2.5 97 2.0 98 1.5 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21147218299295334 2 0.24892800433188694 3 0.30426566467390115 4 0.28908210828253855 5 0.3234280445774581 6 0.2321437718149289 7 0.2241861247905401 8 0.2033316015542108 9 0.20310293353626857 10-11 0.145752994636363 12-13 0.17095221021359422 14-15 0.18798797755028865 16-17 0.16159968827975793 18-19 0.18661596944263545 20-21 0.18320881597529654 22-23 0.2051380788959542 24-25 0.19523675371905666 26-27 0.24817339987267764 28-29 0.17431363007734466 30-31 0.15675192629938314 32-33 0.1948480180885549 34-35 0.15348197364280958 36-37 0.15297890400333672 38-39 0.1858156313798377 40-41 0.20600701736413463 42-43 0.27049139842383707 44-45 0.17248428593380702 46-47 0.15787239958729998 48-49 0.1608908174241371 50-51 0.1667675854852518 52-53 0.1864787686318701 54-55 0.17278155435713188 56-57 0.17054060778129823 58-59 0.14737653756375263 60-61 0.15974747733442607 62-63 0.14851987765346367 64-65 0.14351204806052933 66-67 0.13162131112753456 68-69 0.13527999941460989 70-71 0.13605747067561338 72-73 0.11373947212445394 74-75 0.11440260937648636 76-77 0.1361946714863787 78-79 0.14310044562823335 80-81 0.15199563152618523 82-83 0.1535048404446038 84-85 0.1422772407636414 86-87 0.16736212233190156 88-89 0.1633375652161187 90-91 0.15126389386877018 92-93 0.1511952934633875 94-95 0.17088360980821157 96-97 0.1613252866582273 98-99 0.15332190603025003 100-101 0.13527999941460989 102-103 0.1729187551678972 104-105 0.1318042455418883 106-107 0.1386185524765661 108-109 0.14856561125705212 110-111 0.12469267018388568 112-113 0.11620908671822977 114-115 0.11341933689933487 116-117 0.11771829563664836 118-119 0.10214600361478403 120-121 0.09469142622986806 122-123 0.12206298797755029 124-125 0.11845003329406341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2186576.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.03524593661597 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49395776813805 38.528527014254244 2 11.294686259127987 11.528541820230275 3 4.760438493424191 7.288504478341114 4 2.5210533369444166 5.146503082811386 5 1.4967610637369222 3.8193784498082377 6 0.9819944249767987 3.006979619224601 7 0.6744855127455621 2.4095773816558 8 0.4860029609425572 1.9842624510101574 9 0.3811315901699073 1.7506029994681267 >10 1.766939476529176 15.664450664145205 >50 0.0960823891297932 3.3657802086595447 >100 0.04429120136035464 3.8472595158810483 >500 0.0015134071473856979 0.502668740284898 >1k 5.675276802696345E-4 0.5512315706629437 >5k 0.0 0.0 >10k+ 9.458794671160575E-5 0.6057320035623626 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12548 0.5738652578277637 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.13756667959403196 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.573360358844147E-5 0.0 5 0.0 0.0 0.0 4.573360358844147E-5 0.0 6 0.0 0.0 0.0 4.573360358844147E-5 0.0 7 4.573360358844147E-5 0.0 0.0 4.573360358844147E-5 0.0 8 4.573360358844147E-5 0.0 0.0 4.573360358844147E-5 0.0 9 4.573360358844147E-5 0.0 0.0 1.3720081076532443E-4 0.0 10-11 4.573360358844147E-5 0.0 0.0 1.3720081076532443E-4 0.0 12-13 6.860040538266221E-5 0.0 0.0 1.3720081076532443E-4 0.0 14-15 9.146720717688295E-5 0.0 0.0 1.829344143537659E-4 0.0 16-17 9.146720717688295E-5 0.0 0.0 2.9726842332486957E-4 0.0 18-19 1.1433400897110368E-4 0.0 0.0 4.8020283767863544E-4 0.0 20-21 1.3720081076532443E-4 0.0 0.0 5.716700448555184E-4 0.0 22-23 1.3720081076532443E-4 0.0 0.0 8.918052699746087E-4 0.0 24-25 1.829344143537659E-4 0.0 0.0 0.0014406085130359064 0.0 26-27 2.0580121614798664E-4 0.0 0.0 0.002195212972245191 0.0 28-29 2.9726842332486957E-4 0.0 0.0 0.003292819458367786 0.0 30-31 3.201352251190903E-4 4.573360358844147E-5 0.0 0.006356970898793364 0.0 32-33 3.43002026913311E-4 4.573360358844147E-5 0.0 0.011250466482756603 0.0 34-35 4.116024322959733E-4 4.573360358844147E-5 0.0 0.017401636165401982 0.0 36-37 4.573360358844147E-4 4.573360358844147E-5 0.0 0.027531629360241765 0.0 38-39 4.573360358844147E-4 4.573360358844147E-5 0.0 0.047791615749921336 0.0 40-41 4.573360358844147E-4 4.573360358844147E-5 0.0 0.07367683538097922 0.0 42-43 4.573360358844147E-4 4.573360358844147E-5 0.0 0.0952630962747236 0.0 44-45 4.573360358844147E-4 4.573360358844147E-5 0.0 0.12105684869860457 0.0 46-47 5.030696394728563E-4 4.573360358844147E-5 0.0 0.15092089184185686 0.0 48-49 5.488032430612977E-4 4.573360358844147E-5 0.0 0.18796511074849445 0.0 50-51 5.488032430612977E-4 4.573360358844147E-5 0.0 0.23045162848215656 0.0 52-53 5.488032430612977E-4 4.573360358844147E-5 0.0 0.27385281828758756 0.0 54-55 5.945368466497391E-4 6.860040538266221E-5 0.0 0.31588199998536526 0.0 56-57 6.402704502381806E-4 9.146720717688295E-5 0.0 0.36127260154689345 0.0 58-59 6.402704502381806E-4 9.146720717688295E-5 0.0 0.42143515706748813 0.0 60-61 6.402704502381806E-4 9.146720717688295E-5 0.0 0.49257377744930886 0.0 62-63 6.402704502381806E-4 9.146720717688295E-5 0.0 0.5606253795889098 0.0 64-65 6.402704502381806E-4 9.146720717688295E-5 0.0 0.6295001865931027 0.0 66-67 6.860040538266221E-4 9.146720717688295E-5 0.0 0.7076589151257491 0.0 68-69 7.317376574150636E-4 9.146720717688295E-5 0.0 0.8042025523009491 0.0 70-71 7.546044592092843E-4 9.146720717688295E-5 0.0 0.9172788871733706 0.0 72-73 8.003380627977257E-4 9.146720717688295E-5 0.0 1.030743957676294 0.0 74-75 8.232048645919464E-4 9.146720717688295E-5 0.0 1.1421738828195316 0.0 76-77 8.232048645919464E-4 9.146720717688295E-5 0.0 1.2631849979145477 0.0 78-79 8.232048645919464E-4 9.146720717688295E-5 0.0 1.4104929350729176 0.0 80-81 9.146720717688294E-4 9.146720717688295E-5 0.0 1.581971081727779 0.0 82-83 9.146720717688294E-4 9.146720717688295E-5 0.0 1.770210594097804 0.0 84-85 9.146720717688294E-4 9.146720717688295E-5 0.0 1.9493491193537293 0.0 86-87 9.146720717688294E-4 9.146720717688295E-5 0.0 2.1343186790671806 0.0 88-89 9.146720717688294E-4 9.146720717688295E-5 0.0 2.350341355617184 0.0 90-91 9.146720717688294E-4 9.146720717688295E-5 0.0 2.6054433964335106 0.0 92-93 9.146720717688294E-4 9.146720717688295E-5 0.0 2.8838924418817364 0.0 94-95 9.146720717688294E-4 9.146720717688295E-5 0.0 3.1658401080044785 0.0 96-97 9.146720717688294E-4 9.146720717688295E-5 0.0 3.450143054712025 0.0 98-99 9.146720717688294E-4 9.146720717688295E-5 0.0 3.754705987809251 0.0 100-101 0.0010747396843283747 9.146720717688295E-5 0.0 4.122747162687233 0.0 102-103 0.0012348072968879197 9.146720717688295E-5 0.0 4.523396396923775 0.0 104-105 0.0016006761255954515 9.146720717688295E-5 0.0 4.920204008458887 0.0 106-107 0.0016464097291838929 9.146720717688295E-5 0.0 5.314061802562545 0.0 108-109 0.0016464097291838929 9.146720717688295E-5 0.0 5.724566628372396 0.0 110-111 0.0016464097291838929 9.146720717688295E-5 0.0 6.200401906908335 0.0 112-113 0.0016464097291838929 9.146720717688295E-5 0.0 6.723159862726016 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8220 0.0 47.33038 1 GGTATCA 4960 0.0 39.759865 1 ATCAACG 9760 0.0 38.517513 3 CAACGCA 9970 0.0 37.52694 5 TCAACGC 9940 0.0 37.520325 4 GTACATG 13750 0.0 37.39441 1 TATCAAC 10630 0.0 36.31787 2 AACGCAG 10095 0.0 36.3174 6 TACATGG 13635 0.0 36.266167 2 ACGCATA 320 0.0 33.47136 7 ACATGGG 14280 0.0 32.792385 3 CGCATAG 315 0.0 32.10994 8 ACGCAGA 11985 0.0 30.137102 7 AACGCAT 375 0.0 28.568121 6 CGCAGAG 11940 0.0 28.453314 8 CATGGGA 9275 0.0 28.070244 4 ATGGGAT 3300 0.0 26.177776 5 GTATAGG 570 0.0 26.131466 1 GTATAGA 720 0.0 25.652388 1 GCAGAGT 12600 0.0 25.120728 9 >>END_MODULE