FastQCFastQC Report
Thu 26 May 2016
SRR1364609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186576
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159260.7283533707495189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134930.6170835132188408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96480.4412378074212834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.19642582741235612No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34400.15732359634423868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78200.063.8482131
GTATCAA131500.048.2089161
ATCAACG171350.036.2147453
TATCAAC174700.035.8546872
TCAACGC175550.035.3838234
CAACGCA176500.034.6269875
AACGCAG179600.034.0546726
GTACATG126050.031.3860531
GCGCGAC800.005338821629.7777929
TACATGG129650.029.6676482
ACGCAGA211200.028.8747777
CGCAGAG211450.028.6434868
ACATGGG134800.027.4527423
GAGTACT142350.027.0878812-13
GCAGAGT224950.026.1043859
GTACTTT150750.025.4204914-15
AGTACTT145900.024.3879512-13
CAGAGTA219750.024.33559210-11
CATGGGA88100.024.3126164
AGAGTAC215100.023.22731810-11
Produced by FastQC (version 0.11.5)