##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364609_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186576 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.403536853967115 31.0 30.0 33.0 26.0 34.0 2 29.553698110653368 31.0 30.0 34.0 25.0 34.0 3 29.55423044979914 31.0 30.0 34.0 25.0 34.0 4 33.18511087654854 35.0 33.0 37.0 27.0 37.0 5 32.589694572701795 35.0 33.0 37.0 25.0 37.0 6 32.54577613583978 35.0 33.0 37.0 25.0 37.0 7 32.33304033337968 35.0 33.0 37.0 23.0 37.0 8 32.257364024849814 35.0 33.0 37.0 22.0 37.0 9 33.50798371517843 38.0 33.0 39.0 19.0 39.0 10-11 33.29374556384045 37.0 33.0 39.0 18.0 39.0 12-13 33.13765105809265 37.0 32.5 39.0 17.0 39.0 14-15 34.06895758482669 38.0 33.0 41.0 17.0 41.0 16-17 34.00456558564623 38.0 33.0 41.0 17.0 41.0 18-19 34.079758947322205 38.5 33.0 41.0 17.5 41.0 20-21 34.11281450999188 39.0 33.0 41.0 18.0 41.0 22-23 34.09649172953513 39.0 33.0 41.0 18.0 41.0 24-25 34.0844514437184 39.0 33.0 41.0 18.0 41.0 26-27 33.96244425073722 38.5 33.0 41.0 17.5 41.0 28-29 33.69917533166009 38.0 32.0 41.0 16.0 41.0 30-31 33.57021045689699 38.0 32.5 40.0 16.0 41.0 32-33 33.31301976240478 38.0 32.0 40.0 14.0 41.0 34-35 33.13364548042236 38.0 32.0 40.0 9.5 41.0 36-37 32.928353507950334 38.0 32.0 40.0 9.0 41.0 38-39 32.757951244319884 38.0 31.5 40.0 8.0 41.0 40-41 32.53581672898632 38.0 31.0 40.0 4.0 41.0 42-43 32.30522812836142 38.0 31.0 40.0 2.0 41.0 44-45 32.02668647236593 38.0 30.5 40.0 2.0 41.0 46-47 31.665284902056914 37.0 30.0 40.0 2.0 41.0 48-49 31.325022317998553 37.0 30.0 40.0 2.0 41.0 50-51 31.0926985844535 37.0 29.0 40.0 2.0 41.0 52-53 30.917182389269797 37.0 29.0 40.0 2.0 41.0 54-55 30.690892290046172 36.0 28.5 40.0 2.0 41.0 56-57 30.453427642121746 36.0 28.0 40.0 2.0 41.0 58-59 30.253834076656837 36.0 28.0 40.0 2.0 41.0 60-61 30.02900653807597 35.0 28.0 39.5 2.0 41.0 62-63 29.761748505425835 35.0 28.0 39.0 2.0 41.0 64-65 29.490904500918333 35.0 28.0 39.0 2.0 41.0 66-67 29.208216407753493 35.0 27.0 38.5 2.0 40.0 68-69 28.89882560679345 35.0 26.5 38.0 2.0 40.0 70-71 28.645737445211143 34.0 26.0 37.0 2.0 40.0 72-73 28.493332955268876 34.0 26.0 37.0 2.0 39.0 74-75 28.221982908437667 34.0 26.0 36.5 2.0 39.0 76-77 27.944851676776842 34.0 26.0 36.0 2.0 39.0 78-79 27.65951789464441 34.0 26.0 35.5 2.0 37.5 80-81 27.40249961583773 34.0 26.0 35.0 2.0 37.0 82-83 27.168323671347352 34.0 26.0 35.0 2.0 37.0 84-85 27.159260871792245 34.0 26.0 35.0 2.0 36.0 86-87 27.022056402338634 34.0 26.0 35.0 2.0 36.0 88-89 26.886884105560476 34.0 25.5 35.0 2.0 36.0 90-91 26.745026927945794 34.0 25.0 35.0 2.0 35.5 92-93 26.612918096604005 34.0 25.0 35.0 2.0 35.0 94-95 26.526426476829528 34.0 25.0 35.0 2.0 35.0 96-97 26.448341836734695 34.0 25.0 35.0 2.0 35.0 98-99 26.356626296090326 34.0 24.0 35.0 2.0 35.0 100-101 26.253729118036603 34.0 24.0 35.0 2.0 35.0 102-103 25.099132616474343 31.5 21.5 34.0 2.0 35.0 104-105 25.99812332157675 33.0 23.5 35.0 2.0 35.0 106-107 26.165815869194574 34.0 24.0 35.0 2.0 35.0 108-109 26.16865889866165 34.0 24.0 35.0 2.0 35.0 110-111 26.1015777635902 34.0 24.0 35.0 2.0 35.0 112-113 26.04558131068849 34.0 23.0 35.0 2.0 35.0 114-115 25.95681535880756 34.0 23.0 35.0 2.0 35.0 116-117 25.841543810962893 34.0 23.0 35.0 2.0 35.0 118-119 25.732275027257227 34.0 20.0 35.0 2.0 35.0 120-121 25.591471094533187 34.0 19.0 35.0 2.0 35.0 122-123 25.402023300356355 34.0 17.0 35.0 2.0 35.0 124-125 24.621051131998158 33.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8046769647497527 1101 2 0.769820335534714 1101 3 0.7379800002690402 1101 4 0.752672397416049 1101 5 0.9400366938173264 1101 6 0.9771203600096428 1101 7 0.8753769502072544 1101 8 1.0593800055109241 1101 9 0.954691202375642 1101 10-11 1.17356991700089 1101 12-13 1.1715646355937395 1101 14-15 1.2425898429114284 1101 16-17 1.352936378023763 1101 18-19 1.234386900792778 1101 20-21 1.3397798048811964 1101 22-23 1.2679746400520173 1101 24-25 1.1692527511717046 1101 26-27 1.3362871225037836 1101 28-29 1.426207773192644 1101 30-31 1.5361973099814819 1101 32-33 1.5357024284115184 1101 34-35 1.4472414008967434 1101 36-37 1.4233911487879993 1101 38-39 1.4737899006528608 1101 40-41 1.5600060064584298 1101 42-43 1.5758553380320777 1101 44-45 1.5645881301460633 1101 46-47 1.666633371875303 1101 48-49 1.7525462102544083 1101 50-51 1.6315398217310104 1101 52-53 1.5772600798846277 1101 54-55 1.6058295833531915 1101 56-57 1.6043562327848804 1101 58-59 1.603635088947403 1101 60-61 1.6259030710362303 1101 62-63 1.492227478834529 1101 64-65 1.4147084333790865 1101 66-67 1.4856446109010015 1101 68-69 1.4502549840559347 1101 70-71 1.4764991085202332 1101 72-73 1.5260445534367157 1101 74-75 1.3982051433853897 1101 76-77 1.3382870938185434 1101 78-79 1.3335449784794697 1101 80-81 1.3702147605724058 1101 82-83 1.438295349270902 1101 84-85 1.4724764529515326 1101 86-87 1.41414794452632 1101 88-89 1.4000310528607365 1101 90-91 1.4905592065925148 1101 92-93 1.4002541262754562 1101 94-95 1.3339656831203826 1101 96-97 1.4651849983348377 1101 98-99 1.5407535767282887 1101 100-101 1.4327200678692797 1101 102-103 1.4072757785580698 1101 104-105 1.3680951401002517 1101 106-107 1.3354027676798808 1101 108-109 1.3799202854103783 1101 110-111 1.4060278970939422 1101 112-113 1.4621725662395484 1101 114-115 1.3661225014766885 1101 116-117 1.3047647862984952 1101 118-119 1.3925178411997372 1101 120-121 1.4282759347630538 1101 122-123 1.5214392279751827 1101 124-125 1.4541156863711926 1102 1 0.3634864302170655 1102 2 -0.5318792300043285 1102 3 -0.002954111873705756 1102 4 0.25506940561847813 1102 5 0.6011540544986289 1102 6 0.4590463062729171 1102 7 0.544035181281636 1102 8 0.5233243375359891 1102 9 0.6485252113965814 1102 10-11 0.7779008925725961 1102 12-13 0.920820317249536 1102 14-15 0.9472620903063245 1102 16-17 1.0185397015353033 1102 18-19 0.9887205703691322 1102 20-21 0.9693738910021992 1102 22-23 0.9829330657038113 1102 24-25 0.9049099407204579 1102 26-27 0.9520452224387057 1102 28-29 1.0111965450498062 1102 30-31 1.0212617482299677 1102 32-33 1.0069017383715106 1102 34-35 0.834997735438705 1102 36-37 0.7371292606140187 1102 38-39 0.5994845673201468 1102 40-41 0.6581138313003336 1102 42-43 0.6048484502821196 1102 44-45 0.3663816344557773 1102 46-47 0.3535358709277183 1102 48-49 0.36183495086111606 1102 50-51 0.3335310894077814 1102 52-53 0.46270210045370064 1102 54-55 0.5257975457814297 1102 56-57 0.3270567592636695 1102 58-59 0.4485776457405315 1102 60-61 0.249559242954021 1102 62-63 0.45026425691742133 1102 64-65 0.3201553359531033 1102 66-67 0.38283502174374107 1102 68-69 0.32036268333380136 1102 70-71 0.19112652299261157 1102 72-73 0.1942956384178025 1102 74-75 0.28848405017235734 1102 76-77 0.3229808838801489 1102 78-79 0.2586527738044566 1102 80-81 0.22472154922985155 1102 82-83 0.22469320268800175 1102 84-85 0.3744808861118756 1102 86-87 0.2712988401774439 1102 88-89 0.3668478966211204 1102 90-91 0.25557846989703137 1102 92-93 0.4147316603850655 1102 94-95 0.23151329555291156 1102 96-97 0.08559655791052734 1102 98-99 0.24357197410943243 1102 100-101 0.1406131266608952 1102 102-103 0.03555139537971641 1102 104-105 0.09443224488391522 1102 106-107 0.3314464227367182 1102 108-109 0.3441797457005151 1102 110-111 0.2883571007133767 1102 112-113 0.31907659736068794 1102 114-115 0.31872404319698333 1102 116-117 0.2715148655959361 1102 118-119 0.2757181687907 1102 120-121 0.20528984379190618 1102 122-123 0.12432106330918558 1102 124-125 0.21192897919918963 1103 1 0.391716725676023 1103 2 -0.8817702646904664 1103 3 0.056005404183824936 1103 4 0.3308671763031583 1103 5 0.5120457663110258 1103 6 0.6842014273563564 1103 7 0.7515826338028688 1103 8 0.7185502872664031 1103 9 0.8588328004119603 1103 10-11 0.9270736198930436 1103 12-13 0.9498745689486725 1103 14-15 1.0272593798925698 1103 16-17 0.9942225182278221 1103 18-19 1.0659196440565992 1103 20-21 1.1446437990628624 1103 22-23 1.0049466836242331 1103 24-25 0.9942881250390982 1103 26-27 1.112059461654674 1103 28-29 1.2721315614482904 1103 30-31 1.2165410950202542 1103 32-33 1.1750591485138955 1103 34-35 1.3603948850187138 1103 36-37 1.3146230917981967 1103 38-39 1.2684540995017812 1103 40-41 1.3670427836252017 1103 42-43 1.3784630972053904 1103 44-45 1.4256353831059343 1103 46-47 1.3904614886801632 1103 48-49 1.424233829284269 1103 50-51 1.4382537467966898 1103 52-53 1.3804534018579169 1103 54-55 1.3150121031846993 1103 56-57 1.265835431552219 1103 58-59 1.2652487899980116 1103 60-61 1.2666992219864888 1103 62-63 1.2401394299568196 1103 64-65 1.1433082343063816 1103 66-67 1.066123364435473 1103 68-69 1.0074063329250222 1103 70-71 1.201865726312299 1103 72-73 1.1130973683107541 1103 74-75 1.0412374822099295 1103 76-77 0.9729581459900452 1103 78-79 1.013272430698521 1103 80-81 1.0532094294333767 1103 82-83 1.0846205464204814 1103 84-85 1.1166186104427531 1103 86-87 1.1029096610272084 1103 88-89 1.05123166639628 1103 90-91 1.0835315205679308 1103 92-93 1.1509997164887231 1103 94-95 1.0307415911273523 1103 96-97 1.032764819728861 1103 98-99 1.1007164195983847 1103 100-101 1.05375324121405 1103 102-103 1.0650980071000617 1103 104-105 1.1250919395519858 1103 106-107 1.0074062456245407 1103 108-109 0.9886447515388816 1103 110-111 1.0286799930684332 1103 112-113 1.046466569116074 1103 114-115 1.0867656881318233 1103 116-117 1.0132319031709578 1103 118-119 1.0649581601822753 1103 120-121 1.1194472701586022 1103 122-123 1.1635701450849432 1103 124-125 0.9676780387858201 1104 1 0.45660370866874445 1104 2 -0.5523954993661704 1104 3 0.24211682129460854 1104 4 0.3318852411174831 1104 5 0.6232283068604119 1104 6 0.5696799642268715 1104 7 0.7598570941014842 1104 8 0.6689639024652791 1104 9 0.8687106260715254 1104 10-11 0.9897353732727154 1104 12-13 0.8365736773099002 1104 14-15 0.9784510519930052 1104 16-17 1.0516105664900408 1104 18-19 0.9563513442267109 1104 20-21 1.0457837897719315 1104 22-23 1.0530042367716845 1104 24-25 0.9715715636024669 1104 26-27 0.9604168371868909 1104 28-29 1.3126412407517734 1104 30-31 1.07584278449783 1104 32-33 1.1864519772462216 1104 34-35 1.0724482511531264 1104 36-37 1.1873900220829299 1104 38-39 1.1866604809934174 1104 40-41 1.0807545013083981 1104 42-43 1.053643185173918 1104 44-45 1.0323792773186966 1104 46-47 1.0289384134662818 1104 48-49 1.1421074810562217 1104 50-51 1.1070299361366835 1104 52-53 0.870006869948817 1104 54-55 1.1388199037231246 1104 56-57 1.1503860472053304 1104 58-59 0.9287585869616315 1104 60-61 0.9744777753106675 1104 62-63 0.955748100805188 1104 64-65 0.9663488064252448 1104 66-67 1.0044447528437495 1104 68-69 1.0563964011913356 1104 70-71 1.0321712723232679 1104 72-73 0.9838772018333906 1104 74-75 0.9862465129764111 1104 76-77 0.9658204887954085 1104 78-79 0.984903261027462 1104 80-81 1.0112233733305906 1104 82-83 0.9315103110674094 1104 84-85 0.955781104706233 1104 86-87 1.0074273880960227 1104 88-89 0.9150276446699444 1104 90-91 0.861431901346112 1104 92-93 0.8907168071615565 1104 94-95 0.9020859588869747 1104 96-97 0.9414213707495271 1104 98-99 1.0119047259628609 1104 100-101 0.9018334826095113 1104 102-103 0.9622316469747503 1104 104-105 0.8861013415807122 1104 106-107 0.9869529306793119 1104 108-109 0.9045455570185617 1104 110-111 0.8313707067778999 1104 112-113 0.9148528862274681 1104 114-115 0.9066293898882414 1104 116-117 0.9182399120287457 1104 118-119 0.8771757817319852 1104 120-121 0.8042130491553792 1104 122-123 0.9130925434015147 1104 124-125 0.9289112294425763 1105 1 0.5126846637930527 1105 2 0.36649096348624255 1105 3 0.47860353985507587 1105 4 0.4405654219944921 1105 5 0.5817167112307544 1105 6 0.5862512342818889 1105 7 0.5550366312224213 1105 8 0.539429686061915 1105 9 0.6358904216555175 1105 10-11 0.8488046760813077 1105 12-13 0.812464158211597 1105 14-15 0.8390492924685873 1105 16-17 0.91747503705448 1105 18-19 0.8424852271837224 1105 20-21 0.856328556851139 1105 22-23 0.83830200261594 1105 24-25 0.8467501745339092 1105 26-27 0.7829864348445668 1105 28-29 0.9273040891977189 1105 30-31 0.7546403132212518 1105 32-33 0.7334034861511824 1105 34-35 0.6757215969510497 1105 36-37 0.7310940677457012 1105 38-39 0.6422158915486591 1105 40-41 0.4663639362612102 1105 42-43 0.611263771738237 1105 44-45 0.7796158658999701 1105 46-47 0.6977685301036196 1105 48-49 0.7249195156108854 1105 50-51 0.7580470816079732 1105 52-53 0.6479340886733596 1105 54-55 0.5905904889416647 1105 56-57 0.5649758336189663 1105 58-59 0.5195585527778057 1105 60-61 0.5575586041940781 1105 62-63 0.5964243677612728 1105 64-65 0.6523117974518087 1105 66-67 0.5561044218859728 1105 68-69 0.6272298693296463 1105 70-71 0.6661904843208859 1105 72-73 0.46986695448007865 1105 74-75 0.6225558689674919 1105 76-77 0.6559894404404361 1105 78-79 0.7124586400451349 1105 80-81 0.5528554098445717 1105 82-83 0.5102796916052306 1105 84-85 0.45189980884931913 1105 86-87 0.3961474875589843 1105 88-89 0.6068248591958216 1105 90-91 0.5100202915879883 1105 92-93 0.35352187082621 1105 94-95 0.39608480377823696 1105 96-97 0.5260160028923409 1105 98-99 0.5281921125941373 1105 100-101 0.5979015907061509 1105 102-103 0.34789278360138454 1105 104-105 0.1760305986471451 1105 106-107 0.5265363846574367 1105 108-109 0.49991747330371794 1105 110-111 0.41787222896399356 1105 112-113 0.28840744834963417 1105 114-115 0.4058140350076833 1105 116-117 0.21952627323594243 1105 118-119 0.19623356245326917 1105 120-121 0.08003717428919543 1105 122-123 0.13382543116701484 1105 124-125 -0.07486113492979385 1106 1 0.5910296661392849 1106 2 0.6284421680560044 1106 3 0.6580514270689726 1106 4 0.6683599141848902 1106 5 0.6935961969862134 1106 6 0.7967731013061936 1106 7 0.7141530447756139 1106 8 0.790575344731856 1106 9 0.9450688760895432 1106 10-11 0.988989808733372 1106 12-13 0.9184062317843029 1106 14-15 1.022010447108201 1106 16-17 1.1085067891111535 1106 18-19 1.0439438426976295 1106 20-21 1.0365051746975098 1106 22-23 0.9884976700093375 1106 24-25 1.0553581192294033 1106 26-27 1.029486019536911 1106 28-29 1.17180571335917 1106 30-31 1.014161646183453 1106 32-33 0.9940947392082862 1106 34-35 0.9888750183890949 1106 36-37 1.017343544449183 1106 38-39 1.0102813747550101 1106 40-41 1.1003601192487409 1106 42-43 1.0803853483357528 1106 44-45 1.017221429735045 1106 46-47 0.8921867153845504 1106 48-49 0.9848794631724687 1106 50-51 1.0636345343892017 1106 52-53 1.1034244291634785 1106 54-55 1.0380210198029545 1106 56-57 0.9091252342752014 1106 58-59 0.9330100368089944 1106 60-61 1.0643324029205843 1106 62-63 1.0884584074505668 1106 64-65 1.0589691502173686 1106 66-67 0.8506089538668817 1106 68-69 0.9512594234299598 1106 70-71 1.0428392290529587 1106 72-73 1.14646522301787 1106 74-75 0.9342607967670027 1106 76-77 0.9086776640379881 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-2.3123516405613778 2216 114-115 -2.3811712899086395 2216 116-117 -2.4705387850173572 2216 118-119 -2.490629874046057 2216 120-121 -2.3928264536188593 2216 122-123 -2.3293827448859297 2216 124-125 -2.3318734269333454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96330.0 3 30372.0 4 13797.0 5 11086.0 6 12669.0 7 15340.0 8 16884.0 9 17294.0 10 18487.0 11 20615.0 12 23996.0 13 26900.0 14 30595.0 15 24715.0 16 15076.0 17 12628.0 18 11721.0 19 10643.0 20 10739.0 21 11068.0 22 12589.0 23 16632.0 24 23015.0 25 30336.0 26 34903.0 27 38540.0 28 44120.0 29 51143.0 30 59417.0 31 69775.0 32 84622.0 33 105187.0 34 135416.0 35 189236.0 36 290607.0 37 419121.0 38 150579.0 39 383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46321978621886 19.213304785251033 13.834593484978624 23.48888194355149 2 15.964034638057925 20.94829006627366 38.21450148291897 24.873173812749442 3 19.269716671179047 25.948880807252984 29.439315166726427 25.342087354841542 4 12.855125200556895 17.088865227596628 35.482150665107945 34.57385890673853 5 12.971097366831962 39.17436732602609 32.88147999048473 14.973055316657213 6 31.622250028354937 37.041290277732045 16.38576984571255 14.950689848200469 7 27.704880921075198 34.36872734756412 19.77009706961801 18.15629466174268 8 27.014931106899553 35.41436474195272 18.310271401497136 19.260432749650594 9 27.019631732372556 15.942333215339879 19.09595977612343 37.94207527616413 10-11 25.25623164278603 27.593965711921843 25.564727790988655 21.585074854303468 12-13 26.436529524145808 24.85031076724222 27.146384200610786 21.566775508001186 14-15 23.20717484523191 25.38937572049973 26.232353259317243 25.171096174951114 16-17 21.79185674407495 28.736249392846673 26.725247763170508 22.746646099907867 18-19 21.678453762327514 26.01999842676116 30.768710108791296 21.53283770212003 20-21 22.68450313193938 25.142257969350673 30.961056009001325 21.21218288970862 22-23 22.333503330962515 24.86942581204361 30.910234390901543 21.886836466092333 24-25 21.944263542634694 25.069240675832898 31.146733523097296 21.839762258435105 26-27 21.719254212979564 25.428683933236258 31.086365166360558 21.765696687423624 28-29 21.67358952682413 25.227161711225786 31.21732137362053 21.881927388329554 30-31 21.742944219638375 25.1020774032094 31.20106504416037 21.953913332991856 32-33 21.64797503585075 25.193671791229388 30.96150895348287 22.196844219436997 34-35 21.97181803879673 25.205984150562337 31.036675606061713 21.785522204579213 36-37 22.123831073131747 25.031264756290394 30.71403009511483 22.130874075463026 38-39 22.01233801157609 25.190228924126124 30.747822165797118 22.049610898500667 40-41 22.083321614094732 25.18577565695765 30.603196642420993 22.127706086526626 42-43 22.03845283996567 25.29318020861227 30.24859632750384 22.419770623918218 44-45 22.111945800191716 25.46602542056622 30.041809660400553 22.380219118841513 46-47 22.321565976689993 25.80016249182796 29.230670023824967 22.64760150765708 48-49 22.41649691799836 25.82247688841773 28.930523935705576 22.830502257878337 50-51 22.583251259913695 26.137215171695612 28.558691660890844 22.720841907499846 52-53 22.84792229667155 26.1098989148773 28.223526038234258 22.81865275021689 54-55 22.550622525812045 26.093307527385285 28.390918038064996 22.96515190873768 56-57 22.49118236766234 26.301355390829663 28.253559981852504 22.95390225965549 58-59 22.593086176743917 25.745709273311334 28.43607997160858 23.225124578336175 60-61 22.684347582704646 25.90367314010581 28.690541741974666 22.721437535214875 62-63 22.539852262166967 25.994774478453987 28.66900121468451 22.796372044694536 64-65 22.481302797045764 25.83646344458434 28.70826143567261 22.973972322697282 66-67 22.53077414185466 26.080227716758987 28.489839822626795 22.899158318759557 68-69 22.671423298961248 25.86929886703367 28.515509057373656 22.943768776631433 70-71 22.43313289819334 25.979133586026737 28.59326636714205 22.99446714863787 72-73 22.719165173040363 26.075559578195968 28.234819550581225 22.970455698182448 74-75 22.48189170122536 26.337372898894234 28.229005521387517 22.951729878492884 76-77 22.585169130886356 26.09899746679832 28.44521602644518 22.870617375870143 78-79 22.634717037738973 26.172054006638778 28.291218204419206 22.90201075120304 80-81 22.357560346483485 26.43157682914559 28.25130114244423 22.959561681926697 82-83 22.511190459040346 26.329815270246016 28.084067644681344 23.074926626032294 84-85 22.4712117740517 26.127370206592882 28.07800432732347 23.323413692031952 86-87 22.401577819065313 26.03735315135058 28.26594254680577 23.295126482778333 88-89 22.40820808423764 26.03234463380189 28.210819107133712 23.34862817482676 90-91 22.312167516701912 26.309536005151436 28.17885131822539 23.199445159921265 92-93 22.31393788988052 26.209781618884314 28.108947693636182 23.367332797598984 94-95 22.307302841215996 26.13133414714353 28.07597157549111 23.485391436149364 96-97 22.341582821404046 26.1665824726602 28.137088119223286 23.354746586712473 98-99 22.24365893146249 26.078484729298346 27.963517703769426 23.714338635469737 100-101 22.06306568555241 26.304313873632186 28.052332394949786 23.580288045865622 102-103 22.340438668180756 25.95134029369246 28.04340399337228 23.664817044754507 104-105 22.18709861387515 26.026822454504522 28.153127041588316 23.632951890032004 106-107 22.178374455002768 26.148135884222988 28.036857431514324 23.63663222925992 108-109 22.208526609178634 26.163359747825904 27.694031263806778 23.934082379188684 110-111 22.12429792463297 26.22838321321276 27.936642743166985 23.710676118987283 112-113 22.182292583758866 26.222841684255783 27.788047939958428 23.80681779202693 114-115 21.79276539569795 26.312306474026215 27.817798775748486 24.07712935452735 116-117 21.78180690612763 26.367399916396906 27.897706951953833 23.953086225521627 118-119 21.744157302625666 26.414702140830265 27.634361859061794 24.206778697482267 120-121 21.772244825149397 26.290642388149816 27.797346652670385 24.139766134030403 122-123 21.78649895086154 26.461453536977785 27.520264745106005 24.231782767054675 124-125 21.63878262567713 26.428527817306495 27.47438801879969 24.45830153821668 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 483.0 1 389.5 2 705.0 3 1358.5 4 1125.5 5 964.0 6 2505.0 7 5749.5 8 8676.0 9 9490.5 10 7169.5 11 4069.0 12 3161.0 13 3149.5 14 3263.0 15 3398.0 16 3567.0 17 3853.5 18 4133.5 19 4359.5 20 4735.0 21 5246.5 22 5929.0 23 6710.5 24 7440.5 25 8774.0 26 11129.0 27 13778.0 28 16543.0 29 19645.0 30 22940.5 31 26769.0 32 30488.5 33 33404.0 34 36124.0 35 39775.0 36 44594.0 37 49479.5 38 52862.0 39 54815.0 40 57151.5 41 60536.5 42 63822.5 43 67974.0 44 72748.0 45 75158.5 46 75667.0 47 77247.5 48 80158.0 49 81894.5 50 83647.0 51 82370.0 52 78518.5 53 76594.0 54 74863.5 55 70697.5 56 64054.0 57 58246.0 58 55516.5 59 51332.0 60 44354.5 61 37743.0 62 31471.0 63 24540.0 64 18393.5 65 14547.0 66 10934.0 67 8152.5 68 6210.0 69 4472.0 70 3354.5 71 2572.5 72 2091.5 73 1966.0 74 1581.0 75 1221.5 76 875.5 77 498.5 78 303.0 79 182.0 80 124.0 81 82.0 82 64.0 83 45.5 84 30.0 85 22.5 86 12.5 87 8.5 88 6.5 89 3.0 90 2.0 91 2.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05748713971067093 2 0.07756419168599674 3 0.0 4 0.007500310988504401 5 0.028171899810479947 6 3.658688287075318E-4 7 4.573360358844147E-5 8 0.0 9 6.402704502381806E-4 10-11 0.05933935065600281 12-13 0.009741257564338033 14-15 0.007294509772356414 16-17 0.05362265020744763 18-19 1.829344143537659E-4 20-21 0.01056446242892998 22-23 0.012393806572467638 24-25 0.0 26-27 0.0 28-29 0.05412571984692048 30-31 0.0 32-33 0.0036815550888695384 34-35 0.0 36-37 3.8873563050175245E-4 38-39 0.0 40-41 2.2866801794220736E-5 42-43 0.003635821485281097 44-45 0.0 46-47 2.058012161479866E-4 48-49 4.5733603588441466E-4 50-51 8.003380627977258E-4 52-53 1.3720081076532443E-4 54-55 0.0 56-57 0.002195212972245191 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.68938468180388E-4 66-67 0.0 68-69 6.86004053826622E-4 70-71 0.0 72-73 0.002881217026071813 74-75 0.0035900878816926556 76-77 6.86004053826622E-4 78-79 0.001372008107653244 80-81 0.0016464097291838929 82-83 1.6006761255954515E-4 84-85 2.2866801794220733E-4 86-87 6.174036484439599E-4 88-89 0.0 90-91 0.0 92-93 0.007728979006446609 94-95 0.0069972413490315445 96-97 0.007203042565179531 98-99 0.0016464097291838929 100-101 6.631372520324014E-4 102-103 1.3720081076532443E-4 104-105 5.716700448555184E-4 106-107 0.004436159548078822 108-109 0.006860040538266221 110-111 0.001051872882534154 112-113 0.0041617579265481736 114-115 7.088708556208428E-4 116-117 0.0024696145937758394 118-119 0.002949817431454475 120-121 0.012851142608352054 122-123 9.146720717688293E-4 124-125 0.0023095469812162944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2186576.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.8735775704619 #Duplication Level Percentage of deduplicated Percentage of total 1 73.29568518447769 32.89039614705814 2 11.618822938323751 10.427563048006656 3 5.043164113285948 6.7891444811432 4 2.85030612890316 5.116137326995957 5 1.6787897412361525 3.766665083892807 6 1.157755037849817 3.1171566299128086 7 0.8182628723209181 2.5702867731885175 8 0.569325887450449 2.0438151498703823 9 0.45457533693741037 1.8358579479311763 >10 2.298370514197884 18.1945378367744 >50 0.14061714273397669 4.349703329585348 >100 0.07062064949003168 5.564259854232804 >500 0.0026754381281768594 0.8592811705331261 >1k 7.20310265278384E-4 0.67125425203074 >5k 1.0290146646834057E-4 0.445501893347498 >10k+ 2.0580293293668113E-4 1.3584390754964806 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15926 0.7283533707495189 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13493 0.6170835132188408 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9648 0.4412378074212834 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4295 0.19642582741235612 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3440 0.15732359634423868 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.573360358844147E-5 0.0 4 0.0 0.0 0.0 9.146720717688295E-5 0.0 5 0.0 0.0 0.0 1.3720081076532443E-4 0.0 6 0.0 0.0 0.0 1.3720081076532443E-4 0.0 7 0.0 0.0 0.0 1.3720081076532443E-4 0.0 8 4.573360358844147E-5 0.0 0.0 1.3720081076532443E-4 0.0 9 4.573360358844147E-5 0.0 0.0 1.3720081076532443E-4 0.0 10-11 4.573360358844147E-5 0.0 0.0 1.829344143537659E-4 0.0 12-13 4.573360358844147E-5 0.0 0.0 2.2866801794220736E-4 0.0 14-15 6.860040538266221E-5 0.0 0.0 2.7440162153064885E-4 0.0 16-17 1.1433400897110368E-4 0.0 0.0 2.7440162153064885E-4 0.0 18-19 1.829344143537659E-4 0.0 0.0 2.9726842332486957E-4 0.0 20-21 1.829344143537659E-4 0.0 0.0 4.116024322959732E-4 0.0 22-23 1.829344143537659E-4 0.0 0.0 8.003380627977257E-4 0.0 24-25 1.829344143537659E-4 2.2866801794220736E-5 0.0 0.0013948749094474648 0.0 26-27 1.829344143537659E-4 4.573360358844147E-5 0.0 0.002218079774039411 0.0 28-29 1.829344143537659E-4 4.573360358844147E-5 0.0 0.0033156862601620065 0.0 30-31 1.829344143537659E-4 4.573360358844147E-5 0.0 0.006494171709558689 0.0 32-33 1.829344143537659E-4 4.573360358844147E-5 0.0 0.011776402924023679 0.0 34-35 3.201352251190903E-4 4.573360358844147E-5 0.0 0.01811050702102282 0.0 36-37 4.116024322959732E-4 4.573360358844147E-5 0.0 0.028034698999714624 0.0 38-39 4.573360358844147E-4 4.573360358844147E-5 0.0 0.04827181858759998 0.0 40-41 4.573360358844147E-4 4.573360358844147E-5 0.0 0.07452290704736537 0.0 42-43 5.25936441267077E-4 4.573360358844147E-5 0.0 0.09647503676981728 0.0 44-45 5.488032430612977E-4 4.573360358844147E-5 0.0 0.12277185883317113 0.0 46-47 6.631372520324013E-4 4.573360358844147E-5 0.0 0.15281883639077717 0.0 48-49 6.860040538266221E-4 4.573360358844147E-5 0.0 0.18926851845076503 0.0 50-51 6.860040538266221E-4 4.573360358844147E-5 0.0 0.2308174973108641 0.0 52-53 6.860040538266221E-4 4.573360358844147E-5 0.0 0.27362415026964537 0.0 54-55 6.860040538266221E-4 4.573360358844147E-5 0.0 0.31471579309386 0.0 56-57 7.77471261003505E-4 4.573360358844147E-5 0.0 0.3598091262320633 0.0 58-59 7.77471261003505E-4 4.573360358844147E-5 0.0 0.42008601576162913 0.0 60-61 7.77471261003505E-4 4.573360358844147E-5 0.0 0.48989836163938505 0.0 62-63 7.77471261003505E-4 4.573360358844147E-5 0.0 0.5566694228785096 0.0 64-65 7.77471261003505E-4 4.573360358844147E-5 0.0 0.6239206869553129 0.0 66-67 8.232048645919464E-4 4.573360358844147E-5 0.0 0.6998842025157141 0.0 68-69 8.232048645919464E-4 4.573360358844147E-5 0.0 0.7952387659976146 0.0 70-71 8.460716663861673E-4 4.573360358844147E-5 0.0 0.9065772239336753 0.0 72-73 8.68938468180388E-4 4.573360358844147E-5 0.0 1.0174126122302631 0.0 74-75 8.68938468180388E-4 4.573360358844147E-5 0.0 1.127630596878407 0.0 76-77 8.68938468180388E-4 4.573360358844147E-5 0.0 1.2469495686406509 0.0 78-79 8.68938468180388E-4 4.573360358844147E-5 0.0 1.3928397640877792 0.0 80-81 0.0010061392789457123 4.573360358844147E-5 0.0 1.5604076876358288 0.0 82-83 0.0010061392789457123 4.573360358844147E-5 0.0 1.7459946509977242 0.0 84-85 0.001029006080739933 4.573360358844147E-5 0.0 1.9222976928311661 0.0 86-87 0.0010518728825341537 4.573360358844147E-5 0.0 2.104911971959813 0.0 88-89 0.0010518728825341537 4.573360358844147E-5 0.0 2.3184879007178347 0.0 90-91 0.0010976064861225954 4.573360358844147E-5 0.0 2.5695425176165845 0.0 92-93 0.0010976064861225954 4.573360358844147E-5 0.0 2.844950278426179 0.0 94-95 0.0010976064861225954 4.573360358844147E-5 0.0 3.1238337931084947 0.0 96-97 0.0010976064861225954 4.573360358844147E-5 0.0 3.4045237851325543 0.0 98-99 0.0010976064861225954 4.573360358844147E-5 0.0 3.7047877594924667 0.0 100-101 0.0011433400897110368 4.573360358844147E-5 0.0 4.069033045272609 0.0 102-103 0.001211940495093699 4.573360358844147E-5 0.0 4.466229392438223 0.0 104-105 0.00155494252200701 4.573360358844147E-5 0.0 4.861962264289007 0.0 106-107 0.00155494252200701 4.573360358844147E-5 0.0 5.252915974564798 0.0 108-109 0.0015778093238012307 4.573360358844147E-5 0.0 5.662048792266996 0.0 110-111 0.0016235429273896722 4.573360358844147E-5 0.0 6.136694997109636 0.0 112-113 0.0016464097291838929 4.573360358844147E-5 0.0 6.655771397838447 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7820 0.0 63.848213 1 GTATCAA 13150 0.0 48.208916 1 ATCAACG 17135 0.0 36.214745 3 TATCAAC 17470 0.0 35.854687 2 TCAACGC 17555 0.0 35.383823 4 CAACGCA 17650 0.0 34.626987 5 AACGCAG 17960 0.0 34.054672 6 GTACATG 12605 0.0 31.386053 1 GCGCGAC 80 0.0053388216 29.777792 9 TACATGG 12965 0.0 29.667648 2 ACGCAGA 21120 0.0 28.874777 7 CGCAGAG 21145 0.0 28.643486 8 ACATGGG 13480 0.0 27.452742 3 GAGTACT 14235 0.0 27.08788 12-13 GCAGAGT 22495 0.0 26.104385 9 GTACTTT 15075 0.0 25.42049 14-15 AGTACTT 14590 0.0 24.38795 12-13 CAGAGTA 21975 0.0 24.335592 10-11 CATGGGA 8810 0.0 24.312616 4 AGAGTAC 21510 0.0 23.227318 10-11 >>END_MODULE