FastQCFastQC Report
Thu 26 May 2016
SRR1364608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811355
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26530.14646493923057602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54200.040.004241
GTACATG79000.034.986041
ATCAACG62250.034.8290673
CAACGCA63400.034.5731055
TCAACGC62950.034.347154
AACGCAG64700.033.2974856
TACATGG80450.033.0949172
TATCAAC66300.032.9710162
ACATGGG81250.031.5961173
GGTATCA36900.030.3483681
GTATAGG6450.028.628981
ACGCAGA74850.028.222517
TACCTGG16100.027.3769872
CGCAGAG73800.027.1674278
CATGGGA50600.026.132584
CGCATAG1951.466813E-624.4078969
GCAGAGT80950.023.5184179
AGTACAT58300.021.5572662
GTACCTG21250.021.3038711
ATGGGTA9950.020.9519655
Produced by FastQC (version 0.11.5)