##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364608_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1811355 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.99734176900718 31.0 26.0 31.0 7.0 34.0 2 25.557293297006936 30.0 25.0 31.0 7.0 34.0 3 26.17830850385485 31.0 26.0 31.0 7.0 34.0 4 29.55067670335191 35.0 28.0 37.0 7.0 37.0 5 29.70925246569557 35.0 30.0 37.0 2.0 37.0 6 29.94268931269685 35.0 30.0 37.0 2.0 37.0 7 29.65246348727886 35.0 30.0 37.0 2.0 37.0 8 29.456247394906022 35.0 29.0 37.0 2.0 37.0 9 30.409448175537097 37.0 29.0 39.0 2.0 39.0 10-11 30.344275694162658 36.0 29.0 39.0 2.0 39.0 12-13 30.3488976484455 37.0 29.0 39.0 2.0 39.0 14-15 30.948723469446904 37.0 28.0 40.0 2.0 41.0 16-17 31.321853805576488 37.5 29.5 40.0 2.0 41.0 18-19 31.131228831454905 37.0 28.5 40.0 2.0 41.0 20-21 31.057767803660795 37.0 28.0 40.0 2.0 41.0 22-23 31.101178675632333 37.0 28.5 40.0 2.0 41.0 24-25 31.19177052538017 37.5 29.0 40.0 2.0 41.0 26-27 31.012632808035974 37.0 28.5 40.0 2.0 41.0 28-29 30.903221621382887 37.0 28.5 40.0 2.0 41.0 30-31 30.76495827709091 37.0 28.5 40.0 2.0 41.0 32-33 30.678561905313977 37.0 28.0 40.0 2.0 41.0 34-35 30.728903500418195 37.0 29.0 40.0 2.0 41.0 36-37 30.653770243822994 37.0 28.5 40.0 2.0 41.0 38-39 30.36110563638823 37.0 27.5 40.0 2.0 41.0 40-41 30.231105719199164 37.0 27.0 40.0 2.0 41.0 42-43 29.718192734168618 36.0 25.5 40.0 2.0 41.0 44-45 29.553087329651007 36.0 26.0 40.0 2.0 41.0 46-47 29.505066925036783 36.0 26.0 40.0 2.0 41.0 48-49 29.437614658639525 36.0 26.0 40.0 2.0 41.0 50-51 29.200427856494173 35.5 26.0 40.0 2.0 41.0 52-53 29.10136113572436 35.0 25.5 40.0 2.0 41.0 54-55 28.927108711434258 35.0 25.5 39.5 2.0 41.0 56-57 28.70475334763202 35.0 24.5 39.0 2.0 41.0 58-59 28.504079266626366 35.0 24.0 39.0 2.0 40.0 60-61 28.216698548876394 34.0 23.0 39.0 2.0 40.0 62-63 28.040967949408042 34.0 23.0 39.0 2.0 40.0 64-65 27.818366637130765 34.0 23.0 38.0 2.0 40.0 66-67 27.55198152764091 34.0 22.0 38.0 2.0 40.0 68-69 27.310119772214723 34.0 22.0 37.5 2.0 40.0 70-71 26.877446718064654 33.0 21.0 37.0 2.0 39.5 72-73 26.647596136593876 33.0 21.0 36.5 2.0 39.0 74-75 26.623171603578534 33.0 21.5 36.0 2.0 39.0 76-77 26.376940467219292 33.0 20.5 36.0 2.0 39.0 78-79 26.093224961423907 33.0 20.0 35.5 2.0 37.5 80-81 25.698672540722278 32.5 18.0 35.0 2.0 37.0 82-83 25.810217213080815 33.0 20.0 35.0 2.0 37.0 84-85 25.651871665134664 33.0 20.0 35.0 2.0 36.0 86-87 25.525250986140207 33.0 19.5 35.0 2.0 36.0 88-89 25.36054804276357 33.0 18.5 35.0 2.0 36.0 90-91 25.231684291593865 33.0 18.0 35.0 2.0 35.5 92-93 25.11333118024904 33.0 18.0 35.0 2.0 35.0 94-95 25.009238663873177 33.0 16.5 35.0 2.0 35.0 96-97 24.895732752552647 33.0 13.5 35.0 2.0 35.0 98-99 24.811971148670473 33.0 10.5 35.0 2.0 35.0 100-101 24.70685536518242 32.0 8.5 35.0 2.0 35.0 102-103 23.577119062801053 30.5 7.0 34.0 2.0 35.0 104-105 24.41977221472323 32.0 7.0 34.5 2.0 35.0 106-107 24.579264694110208 32.0 6.0 35.0 2.0 35.0 108-109 24.538270521239625 33.0 2.0 35.0 2.0 35.0 110-111 24.47495576515923 33.0 2.0 35.0 2.0 35.0 112-113 24.38983578591717 32.5 2.0 35.0 2.0 35.0 114-115 24.29492921045295 32.0 2.0 35.0 2.0 35.0 116-117 24.185718426261005 32.0 2.0 35.0 2.0 35.0 118-119 24.06521913153413 32.0 2.0 35.0 2.0 35.0 120-121 23.881407841091338 32.0 2.0 35.0 2.0 35.0 122-123 23.684801433181235 32.0 2.0 35.0 2.0 35.0 124-125 22.76222772454875 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.4491438384185216 1101 2 1.7853599036148573 1101 3 1.480843079668137 1101 4 1.574451121347245 1101 5 1.2900066440551292 1101 6 1.1063158994252547 1101 7 1.2065052685595319 1101 8 1.4270284263270412 1101 9 1.9347415969709338 1101 10-11 1.8155443212311582 1101 12-13 1.777163850351105 1101 14-15 2.151355993879733 1101 16-17 1.8160321247213602 1101 18-19 1.8234554874902678 1101 20-21 1.92766176307698 1101 22-23 1.6694672927351668 1101 24-25 1.6624439272781757 1101 26-27 1.90067763707237 1101 28-29 1.881490590972195 1101 30-31 1.9468867013503832 1101 32-33 2.047561712875069 1101 34-35 1.7671594594276847 1101 36-37 1.6980967349111253 1101 38-39 1.668327815929647 1101 40-41 1.7780853297719723 1101 42-43 1.9940310205774665 1101 44-45 1.7936245992711015 1101 46-47 1.5292902933279002 1101 48-49 1.4955758838968265 1101 50-51 1.4106464266851333 1101 52-53 1.2856906008949238 1101 54-55 1.245593536545556 1101 56-57 1.4698166658318321 1101 58-59 1.2830973794542935 1101 60-61 1.2992174871669668 1101 62-63 1.1318180511544682 1101 64-65 1.0726654257320831 1101 66-67 1.1113577012371536 1101 68-69 1.0421735150741647 1101 70-71 0.8611764991357092 1101 72-73 0.9369419058963473 1101 74-75 0.9399323862659728 1101 76-77 0.9798501879991335 1101 78-79 0.9607260087917524 1101 80-81 1.0836194661248406 1101 82-83 1.143530134724493 1101 84-85 1.2103536032279685 1101 86-87 1.1948873508957902 1101 88-89 1.1577607731399766 1101 90-91 1.1815287358110353 1101 92-93 1.1116587619068525 1101 94-95 1.024652422961168 1101 96-97 1.075613547206114 1101 98-99 1.1256621085406664 1101 100-101 1.149864411424737 1101 102-103 1.0814037125276634 1101 104-105 1.1684335284287997 1101 106-107 1.1618351511064873 1101 108-109 1.1299587024700486 1101 110-111 1.2421056980323577 1101 112-113 1.2327606650020506 1101 114-115 1.22406485830534 1101 116-117 1.0963842801969648 1101 118-119 1.1019582910403365 1101 120-121 1.239451421207221 1101 122-123 1.1047938226959957 1101 124-125 0.8655960797482507 1102 1 0.9055628264345295 1102 2 1.178801957288531 1102 3 0.9332229252991802 1102 4 0.9318949884241334 1102 5 0.6341551374976753 1102 6 0.6623156609922631 1102 7 0.8114376596815127 1102 8 0.6184687912826412 1102 9 1.1168324902524844 1102 10-11 1.1851697353444202 1102 12-13 1.3131568417336084 1102 14-15 1.56079698335477 1102 16-17 1.1793083034061098 1102 18-19 1.0363076546602237 1102 20-21 1.1897119258785942 1102 22-23 1.3094296465719104 1102 24-25 0.7592894205118199 1102 26-27 1.1614808117311028 1102 28-29 0.9419288428427492 1102 30-31 1.0930883708188226 1102 32-33 1.3283815012821982 1102 34-35 1.2580455497062921 1102 36-37 0.9903144132956427 1102 38-39 1.1780461478174296 1102 40-41 1.1961815462341328 1102 42-43 1.5305868724121439 1102 44-45 1.1572026472486279 1102 46-47 0.8643035493272784 1102 48-49 0.7450748191513341 1102 50-51 0.8896539329494679 1102 52-53 0.6949842103438755 1102 54-55 0.7066738622144015 1102 56-57 0.8037513680038693 1102 58-59 0.7983343960669522 1102 60-61 0.7777181866068652 1102 62-63 0.538633816825147 1102 64-65 0.3250191312323416 1102 66-67 0.3681274755898549 1102 68-69 0.18651584469574445 1102 70-71 0.53394813501572 1102 72-73 0.12936717410612175 1102 74-75 0.26786306132664706 1102 76-77 0.1035526148751238 1102 78-79 0.1781736164229173 1102 80-81 0.2750480734902325 1102 82-83 0.2045780039757119 1102 84-85 0.24305405920372536 1102 86-87 0.22698461515063428 1102 88-89 0.15657380339362348 1102 90-91 0.26323113643752194 1102 92-93 0.24179108674883665 1102 94-95 0.20282366378614824 1102 96-97 0.049489822357784874 1102 98-99 0.25153203349989894 1102 100-101 0.22336288512547142 1102 102-103 0.13253747234251634 1102 104-105 0.24844953515742318 1102 106-107 0.25011523987355844 1102 108-109 0.2839488184381693 1102 110-111 0.22498309206912737 1102 112-113 0.09834051107058528 1102 114-115 0.21427475043081756 1102 116-117 0.17636036580534764 1102 118-119 0.24895783604893396 1102 120-121 0.15074231711371766 1102 122-123 -0.03548407205005333 1102 124-125 -0.036588015677445185 1103 1 0.9677094816880469 1103 2 1.084290342480081 1103 3 0.878465133765765 1103 4 0.7945016945025252 1103 5 0.7856396445932319 1103 6 0.8371349744446022 1103 7 0.7896342226020607 1103 8 0.936035764686963 1103 9 1.4966095665198509 1103 10-11 1.4699644878931792 1103 12-13 1.4496311192935245 1103 14-15 1.7618293427487473 1103 16-17 1.4670786931859254 1103 18-19 1.3363970378715457 1103 20-21 1.6188458159183163 1103 22-23 1.465207163633199 1103 24-25 1.3127007591562005 1103 26-27 1.6206874772920727 1103 28-29 1.5154558564973435 1103 30-31 1.6158447742966366 1103 32-33 1.693194820605111 1103 34-35 1.358566042727606 1103 36-37 1.2719056181212274 1103 38-39 1.232578394230135 1103 40-41 1.3753791621623783 1103 42-43 1.603166346108658 1103 44-45 1.3672515241702854 1103 46-47 1.1714182247593605 1103 48-49 1.0796701013206622 1103 50-51 1.1084761115622825 1103 52-53 0.8854412724524039 1103 54-55 0.886193446933099 1103 56-57 0.8918147020755676 1103 58-59 1.0740873077000153 1103 60-61 1.0975280886637933 1103 62-63 0.9037902541416933 1103 64-65 0.910226766317308 1103 66-67 0.8688895426016643 1103 68-69 0.8371204336275859 1103 70-71 0.8271007187248216 1103 72-73 0.8311675835101724 1103 74-75 0.8638424586634059 1103 76-77 0.7451957855191402 1103 78-79 0.6556462365531743 1103 80-81 0.694813144802005 1103 82-83 0.7714336962837507 1103 84-85 0.929340832380749 1103 86-87 0.9553680424124025 1103 88-89 0.9376134592626961 1103 90-91 0.9920401914132029 1103 92-93 0.9203621575329919 1103 94-95 0.9757327032895162 1103 96-97 0.9609063848553525 1103 98-99 0.7875565009017436 1103 100-101 0.7881261977140319 1103 102-103 0.6279579741574501 1103 104-105 0.6609046348425238 1103 106-107 0.7865063398436192 1103 108-109 0.9159743889379222 1103 110-111 0.8984279855940152 1103 112-113 0.9268911561769357 1103 114-115 0.9825394527446498 1103 116-117 1.0314905502820544 1103 118-119 0.9730286346762433 1103 120-121 1.1089889201620977 1103 122-123 1.017755846737007 1103 124-125 1.118361899670088 1104 1 0.7181038120453813 1104 2 1.0516006826499265 1104 3 0.564187651554203 1104 4 0.7648354156303547 1104 5 0.17538999644048303 1104 6 0.4185282459922526 1104 7 0.3261969254861192 1104 8 0.5921682553297849 1104 9 0.8577050641821913 1104 10-11 0.9405095352346073 1104 12-13 0.8114237755986728 1104 14-15 0.8591755648024915 1104 16-17 0.7099565095979088 1104 18-19 0.7099477104117753 1104 20-21 0.888652746546672 1104 22-23 0.6691753579487418 1104 24-25 0.8517522639295549 1104 26-27 1.1623127501643253 1104 28-29 1.1141773329050544 1104 30-31 0.9821289296235882 1104 32-33 1.0867738261083062 1104 34-35 1.0206107654545455 1104 36-37 1.006482889442296 1104 38-39 1.1026135898484952 1104 40-41 1.1065694951863456 1104 42-43 1.2418469951633568 1104 44-45 1.012442164379781 1104 46-47 0.7715993770754572 1104 48-49 0.6428533712561304 1104 50-51 0.6857801119457179 1104 52-53 0.47114331120570796 1104 54-55 0.3987766157805659 1104 56-57 0.42315606798363703 1104 58-59 0.42366116947959753 1104 60-61 0.4050918786796096 1104 62-63 0.37452048424761486 1104 64-65 0.32688362919056146 1104 66-67 0.23418705505248028 1104 68-69 0.07066966827811783 1104 70-71 0.060535848113108415 1104 72-73 0.1344650894251238 1104 74-75 0.07144459240457834 1104 76-77 0.16130036803004444 1104 78-79 0.1677928642661044 1104 80-81 0.27237122683143866 1104 82-83 0.2648425515056836 1104 84-85 0.2304553602954158 1104 86-87 0.20334355907231583 1104 88-89 0.19748397293789566 1104 90-91 0.22139830437905061 1104 92-93 0.19905295896042574 1104 94-95 0.2546726844662359 1104 96-97 0.2133072249315191 1104 98-99 0.23649698316123136 1104 100-101 0.1844957440816195 1104 102-103 0.15159355844174982 1104 104-105 0.12904775279740832 1104 106-107 0.28114148622514534 1104 108-109 0.2609608923823252 1104 110-111 0.17550442903920072 1104 112-113 0.21954038080580673 1104 114-115 0.25480604620965863 1104 116-117 0.20999802077263396 1104 118-119 0.23755758418888462 1104 120-121 0.3037115926326095 1104 122-123 0.27449677169223463 1104 124-125 0.10529888960050116 1105 1 0.8114968647550072 1105 2 1.1477445498353944 1105 3 0.6175615785546462 1105 4 0.8679270246437092 1105 5 0.6032669258745322 1105 6 0.5050283952375807 1105 7 0.4875911424155319 1105 8 0.5164967575759007 1105 9 0.7476068915611123 1105 10-11 0.5985793257494052 1105 12-13 0.8501565234229282 1105 14-15 1.0896294724276032 1105 16-17 0.8777052712865796 1105 18-19 0.8461909027534205 1105 20-21 0.7924082317108407 1105 22-23 0.7929583578966906 1105 24-25 0.5182850214202404 1105 26-27 0.7980149945803703 1105 28-29 0.8860043294198334 1105 30-31 0.8332575775033995 1105 32-33 0.9217375053190153 1105 34-35 0.6082153832431025 1105 36-37 0.344463740894863 1105 38-39 0.6346261153025274 1105 40-41 0.6444361155391611 1105 42-43 0.6699229626289345 1105 44-45 0.5530396157369601 1105 46-47 0.15505152243721554 1105 48-49 0.22891919535059202 1105 50-51 0.559569286188843 1105 52-53 0.271897079783038 1105 54-55 0.4628195920617131 1105 56-57 0.4349680257742996 1105 58-59 0.5289448524506533 1105 60-61 0.4144435458189726 1105 62-63 0.3420605547593958 1105 64-65 0.020702588764727636 1105 66-67 0.1588510937266676 1105 68-69 0.09131571465782784 1105 70-71 0.4221897439473068 1105 72-73 0.39445131477176076 1105 74-75 0.40229871385946936 1105 76-77 0.34060614886992013 1105 78-79 0.3912398763321363 1105 80-81 0.43979773014616796 1105 82-83 0.3976591428061127 1105 84-85 0.23578298034761147 1105 86-87 0.31120730074299274 1105 88-89 0.26197873501109825 1105 90-91 0.14043098751263017 1105 92-93 0.19843672446786442 1105 94-95 0.195662555435419 1105 96-97 0.22515417039483054 1105 98-99 0.31594456034125074 1105 100-101 0.30116549125684244 1105 102-103 0.18484306741931178 1105 104-105 0.26377512247862 1105 106-107 0.3060495224221853 1105 108-109 0.25745210018599707 1105 110-111 0.4020074516554466 1105 112-113 0.28319869185856916 1105 114-115 0.32690415403383355 1105 116-117 0.30415403047305034 1105 118-119 0.4288508586951245 1105 120-121 0.2993079923679005 1105 122-123 0.08531218496370485 1105 124-125 -0.038142867011703885 1106 1 -0.05597664881380027 1106 2 -2.461169115155684 1106 3 -0.8023102039598733 1106 4 -0.13633031302111576 1106 5 -0.06194442966476288 1106 6 -0.010703718533719098 1106 7 0.09003186007771902 1106 8 0.059210601820598896 1106 9 0.4597923848696901 1106 10-11 0.6935644012279241 1106 12-13 0.579188425374749 1106 14-15 0.7629247831231609 1106 16-17 0.4121497123039397 1106 18-19 0.29986294163094485 1106 20-21 0.6131660524659601 1106 22-23 0.2630483342678076 1106 24-25 0.28605211612110537 1106 26-27 0.3214768743992238 1106 28-29 0.4363934490799757 1106 30-31 0.4875513365011024 1106 32-33 0.6582473469183476 1106 34-35 0.4868437974860278 1106 36-37 0.2533755167060079 1106 38-39 0.7870334574525479 1106 40-41 0.7609866940266201 1106 42-43 0.9633180682906399 1106 44-45 0.8479938089067609 1106 46-47 0.5473979533136664 1106 48-49 0.40945102906060526 1106 50-51 0.4650922973000462 1106 52-53 0.5156027049383773 1106 54-55 0.43444488054720054 1106 56-57 0.4063393030938336 1106 58-59 0.38718201110347295 1106 60-61 0.4111988471534538 1106 62-63 0.30504972063376457 1106 64-65 0.36574967304040484 1106 66-67 0.2660669385743617 1106 68-69 0.16779845744893507 1106 70-71 0.27688218416889754 1106 72-73 0.23034911347873788 1106 74-75 0.4069899203308971 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-1.5467152810998002 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 189501.0 3 23724.0 4 14165.0 5 11422.0 6 10661.0 7 11844.0 8 12906.0 9 13738.0 10 14363.0 11 15277.0 12 16323.0 13 16575.0 14 16105.0 15 13840.0 16 11373.0 17 9816.0 18 8866.0 19 8416.0 20 8197.0 21 8833.0 22 11045.0 23 15601.0 24 22806.0 25 29103.0 26 33026.0 27 35987.0 28 41445.0 29 47392.0 30 54225.0 31 62771.0 32 75941.0 33 95421.0 34 124475.0 35 169156.0 36 239255.0 37 256517.0 38 61166.0 39 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87717905671701 18.92822077236031 13.650692679365099 23.54390749155758 2 13.416378515157904 21.1457556928944 41.59386103101523 23.844004760932467 3 19.766265760591256 26.02577055623988 27.619337605358947 26.58862607780992 4 11.662838964732556 16.975844239377782 37.24268183798331 34.11863495790635 5 13.147091590051849 39.35904903865108 33.31448878297058 14.179370588326492 6 31.290342747724598 37.327916586675045 17.79542910824512 13.586311557355234 7 28.404436996955663 33.10365735257264 20.111704955524367 18.380200694947334 8 25.576686196976812 37.042598864244155 19.300935474753576 18.079779464025457 9 25.725946689793716 18.009285480402482 19.83491004345171 36.429857786352095 10-11 24.09768167987546 28.439528861802998 27.07176089725446 20.39102856106708 12-13 24.995845987749465 25.499386920321758 27.4622648421268 22.042502249801977 14-15 21.977450106113082 26.76966894101219 25.423449954029188 25.82943099884554 16-17 22.04429847841472 30.21529989384289 25.605703733191792 22.1346978945506 18-19 21.917733598123682 28.8233530930643 27.695671385664266 21.563241923147753 20-21 22.677684396511083 27.746264096477486 27.944821026407435 21.631230480603993 22-23 22.4472197862159 27.719751507996232 27.53495611455102 22.29807259123685 24-25 22.274865134224218 27.730803818828004 27.718166201535983 22.2761648454118 26-27 22.08389889547305 27.926448609145975 27.76550894505807 22.224143550322903 28-29 21.953042961835724 27.989918265623174 27.92640975349186 22.130629019049238 30-31 22.117362692527347 27.73394502006613 27.84042627886701 22.30826600853951 32-33 21.88856820421097 27.717083662751808 27.876863720441865 22.51748441259536 34-35 22.500985458339024 27.722864236735568 27.636038861058942 22.14011144386647 36-37 22.450936570606085 27.510045988888255 27.531054316755277 22.507963123750386 38-39 22.15298727225972 27.76975592491103 27.808992647607777 22.26826415522147 40-41 22.361860743992274 27.59723594270107 27.863563804190665 22.17733950911599 42-43 22.09451381509033 27.523412592986183 27.838901434643997 22.54317215727949 44-45 22.29064059838063 27.758074317617936 27.58375675497835 22.367528329023088 46-47 22.359215327231258 28.014679925704716 27.07147590144126 22.55462884562277 48-49 22.420191400204903 27.84163067799506 27.22264027012099 22.51553765167905 50-51 22.34922521874957 27.9583373650526 27.190439987282804 22.50199742891503 52-53 22.617354314300854 28.007617343253834 27.00780647723037 22.367221865214944 54-55 22.479208190152473 27.795154397556598 27.169623733283128 22.5560136790078 56-57 22.416750875378938 28.163936737715318 27.020753358095995 22.398559028809746 58-59 22.544894894438364 27.727104495252554 27.0534426766918 22.67455793361728 60-61 22.492634216490178 27.856649606540827 27.317588700398794 22.3331274765702 62-63 22.368147547816932 27.911611507243716 27.327851850705244 22.392389094234105 64-65 22.3524485266274 27.768450671524008 27.179986124509036 22.699114677339555 66-67 22.29007131478552 28.151597258841388 26.97063612877122 22.587695297601872 68-69 22.445408136896777 27.853837992249865 27.17876194048021 22.521991930373144 70-71 22.400866069350776 27.772839760034447 27.1859710915912 22.640323079023577 72-73 22.5561541301277 27.746584109060922 27.086727486952082 22.610534273859287 74-75 22.351767597567765 27.997109614951526 26.989177635714768 22.661945151765938 76-77 22.536239334381378 27.824053498897783 27.12624178866211 22.51346537805873 78-79 22.53973211571098 27.85465922973593 27.121377122040474 22.484231532512617 80-81 22.343754103186768 28.0156401385996 27.132433937829205 22.508171820384423 82-83 22.48157244761295 28.126008107708405 27.031343079353537 22.361076365325104 84-85 22.454840897670696 27.95312087995918 27.01375848331088 22.578279739059244 86-87 22.43461827044667 27.8779765903578 27.255966174399564 22.431438964795962 88-89 22.54066240287951 27.83573868246489 27.113207834052332 22.51039108060327 90-91 22.41041459294639 28.060175365903078 27.16369244156428 22.365717599586254 92-93 22.47017207498645 27.92733548997185 27.223401158813125 22.379091276228575 94-95 22.380471977167797 27.873537080622405 27.20859320591087 22.537397736298924 96-97 22.528607769061203 27.825820609093775 27.143557729999717 22.502013891845298 98-99 22.24572499543889 27.876263980495033 27.020765492572302 22.857245531493778 100-101 22.20034210371397 27.86426182514459 27.28010411804098 22.655291953100452 102-103 22.311128732055113 27.7001792691437 27.309514093253135 22.679177905548052 104-105 22.23277962255912 27.63015381468999 27.432575660217534 22.70449090253336 106-107 22.16877173450994 27.936200662558797 27.27601880990378 22.61900879302749 108-109 22.174718615532306 27.89373578012995 27.020068070177572 22.911477534160166 110-111 22.03646887223514 27.9508188113542 27.257665941327687 22.755046375082976 112-113 22.260361887145216 27.909622442186954 27.25162571780142 22.578389952866413 114-115 22.01989957369109 28.041167491762852 27.180702144144803 22.758230790401253 116-117 21.948227655504667 27.927807434910136 27.355236564066317 22.768728345518888 118-119 22.018004905304743 28.06569803890039 27.09577169583353 22.82052535996134 120-121 21.900581450925333 28.173681596080858 27.222655975882194 22.703080977111618 122-123 22.02684027018789 28.08528665389769 27.133444442387212 22.75442863352721 124-125 22.019293741830833 28.03998021438105 27.148632553975222 22.792093489812892 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3418.0 1 2575.0 2 1395.0 3 940.5 4 747.0 5 653.0 6 612.0 7 683.5 8 867.0 9 973.5 10 952.0 11 917.0 12 949.0 13 1031.5 14 1131.5 15 1228.0 16 1337.5 17 1484.5 18 1664.5 19 1881.0 20 2234.0 21 2730.0 22 3433.5 23 4381.0 24 5591.0 25 7112.0 26 9378.0 27 12155.5 28 14766.0 29 17471.0 30 20881.5 31 25099.0 32 29713.0 33 33896.5 34 37984.5 35 41681.0 36 45762.0 37 50384.0 38 53446.0 39 55307.5 40 57009.0 41 59512.0 42 61384.5 43 63061.0 44 65448.5 45 66959.5 46 66821.5 47 67573.5 48 70084.0 49 70123.0 50 67780.5 51 65373.0 52 62480.0 53 59988.5 54 57419.0 55 52797.5 56 47645.0 57 43180.5 58 39395.0 59 34959.5 60 29401.5 61 24078.0 62 19131.0 63 14620.0 64 11082.0 65 8501.0 66 6545.5 67 5122.0 68 3987.5 69 3057.0 70 2321.5 71 1740.0 72 1336.5 73 1109.0 74 881.0 75 643.0 76 460.5 77 318.0 78 224.5 79 167.5 80 126.5 81 85.0 82 55.0 83 46.5 84 40.0 85 28.5 86 26.0 87 18.0 88 12.5 89 13.5 90 10.5 91 4.0 92 3.0 93 4.5 94 3.5 95 1.5 96 2.5 97 3.0 98 2.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19432965928821241 2 0.22888942255935474 3 0.2776926665396899 4 0.2643325024636253 5 0.29541420649182515 6 0.2138730397961747 7 0.20625443383544365 8 0.18748395538146856 9 0.1861037731422057 10-11 0.1357547250538961 12-13 0.15803086639559888 14-15 0.17348890747534304 16-17 0.14988779118394793 18-19 0.17290923093485264 20-21 0.16879628786184928 22-23 0.18822925379067051 24-25 0.18003097128944906 26-27 0.2294414954550599 28-29 0.1620886021790317 30-31 0.1427660508293515 32-33 0.17912005101153558 34-35 0.14088900298395401 36-37 0.1406957774704572 38-39 0.17114259766859616 40-41 0.1895266250955776 42-43 0.2558857871593365 44-45 0.1586657502256598 46-47 0.14500194605695738 48-49 0.14817636520726196 50-51 0.1534762650060314 52-53 0.17186029243301285 54-55 0.1595766705035733 56-57 0.15747879349989372 58-59 0.134457353748989 60-61 0.14652014652014653 62-63 0.13674845626616539 64-65 0.1330495678649409 66-67 0.12106958602813916 68-69 0.1228638229391809 70-71 0.12487888900850469 72-73 0.10329283878643336 74-75 0.10530790485575715 76-77 0.12537575461463932 78-79 0.1305652398342677 80-81 0.1406405701808867 82-83 0.14257282531585472 84-85 0.13039961796555616 86-87 0.15369709416431346 88-89 0.14974977296002165 90-91 0.1384874858876366 92-93 0.13920518065205328 94-95 0.1576996226581758 96-97 0.14729304857413372 98-99 0.142710843539781 100-101 0.12446483433672581 102-103 0.1601563470440637 104-105 0.12131801883120646 106-107 0.12741842432874836 108-109 0.13727292551708528 110-111 0.11516240604409406 112-113 0.1061912214888854 114-115 0.10318242420729233 116-117 0.10886877503305536 118-119 0.09283105741282079 120-121 0.08678585920484941 122-123 0.11160153586679586 124-125 0.10820628755820919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1811355.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.47786574719922 #Duplication Level Percentage of deduplicated Percentage of total 1 85.28820938306548 60.109309707187066 2 8.95177285739718 12.61803691286121 3 2.6134818756094793 5.525778742858298 4 1.050696600893046 2.962034159151146 5 0.5722815744508861 2.0166591986872677 6 0.3287792055621806 1.390299402604931 7 0.2244666129885709 1.1073949470455917 8 0.15704935727549918 0.8854802814197247 9 0.12221501740247241 0.775210822990476 >10 0.6333919816039837 8.069005925316437 >50 0.03956337274658025 1.8915088680736127 >100 0.017607689996034345 2.207679055590038 >500 2.4223550426064706E-4 0.12405828713233877 >1k 2.42235504260647E-4 0.3175436890818579 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2653 0.14646493923057602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 5.5207289570514894E-5 0.0 0.0 9 0.0 0.0 5.5207289570514894E-5 0.0 0.0 10-11 0.0 0.0 5.5207289570514894E-5 0.0 0.0 12-13 0.0 0.0 5.5207289570514894E-5 0.0 0.0 14-15 0.0 0.0 5.5207289570514894E-5 0.0 0.0 16-17 0.0 0.0 5.5207289570514894E-5 0.0 0.0 18-19 0.0 0.0 5.5207289570514894E-5 2.7603644785257447E-5 0.0 20-21 0.0 0.0 5.5207289570514894E-5 1.1041457914102979E-4 0.0 22-23 0.0 0.0 5.5207289570514894E-5 1.6562186871154467E-4 0.0 24-25 0.0 0.0 5.5207289570514894E-5 1.6562186871154467E-4 0.0 26-27 0.0 0.0 5.5207289570514894E-5 2.48432803067317E-4 0.0 28-29 0.0 0.0 5.5207289570514894E-5 8.557129883429808E-4 0.0 30-31 0.0 0.0 5.5207289570514894E-5 0.0022911025171763683 0.0 32-33 0.0 0.0 5.5207289570514894E-5 0.004692619613493766 0.0 34-35 0.0 0.0 5.5207289570514894E-5 0.007922246053368887 0.0 36-37 0.0 2.7603644785257447E-5 5.5207289570514894E-5 0.012725280246003683 0.0 38-39 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.021862086669923897 0.0 40-41 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.03469778149506861 0.0 42-43 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.04747826903064281 0.0 44-45 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.061197280488915755 0.0 46-47 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.08007817352203185 0.0 48-49 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.09981477954349093 0.0 50-51 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.12264299378089882 0.0 52-53 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.14621650642750866 0.0 54-55 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.17100457944466987 0.0 56-57 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.20103734497102999 0.0 58-59 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.23529346814953445 0.0 60-61 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.2765885207482796 0.0 62-63 0.0 5.5207289570514894E-5 5.5207289570514894E-5 0.3165309947525471 0.0 64-65 0.0 1.3801822392628724E-4 5.5207289570514894E-5 0.35840572389178266 2.7603644785257447E-5 66-67 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.4064636694629159 5.5207289570514894E-5 68-69 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.46520422556594376 5.5207289570514894E-5 70-71 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.5335784536990265 5.5207289570514894E-5 72-73 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.604298991638856 5.5207289570514894E-5 74-75 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.6743570421038394 5.5207289570514894E-5 76-77 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.7508467418037879 5.5207289570514894E-5 78-79 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.8473490839730478 5.5207289570514894E-5 80-81 0.0 1.6562186871154467E-4 5.5207289570514894E-5 0.9571563829288019 5.5207289570514894E-5 82-83 0.0 1.6562186871154467E-4 5.5207289570514894E-5 1.0773426523238128 5.5207289570514894E-5 84-85 0.0 1.6562186871154467E-4 5.5207289570514894E-5 1.191235290707785 5.5207289570514894E-5 86-87 0.0 1.6562186871154467E-4 5.5207289570514894E-5 1.3171355145733443 5.5207289570514894E-5 88-89 0.0 1.6562186871154467E-4 5.5207289570514894E-5 1.464538977726619 5.5207289570514894E-5 90-91 0.0 1.6562186871154467E-4 5.5207289570514894E-5 1.6362336482909203 5.5207289570514894E-5 92-93 0.0 1.6562186871154467E-4 5.5207289570514894E-5 1.8203223553638024 5.5207289570514894E-5 94-95 0.0 1.6562186871154467E-4 5.5207289570514894E-5 2.0070058050464983 5.5207289570514894E-5 96-97 0.0 1.9322551349680212E-4 5.5207289570514894E-5 2.199624038358025 5.5207289570514894E-5 98-99 0.0 2.2082915828205957E-4 5.5207289570514894E-5 2.416119424408799 5.5207289570514894E-5 100-101 0.0 2.2082915828205957E-4 5.5207289570514894E-5 2.675041612494514 5.5207289570514894E-5 102-103 0.0 2.2082915828205957E-4 5.5207289570514894E-5 2.9555498508023 5.5207289570514894E-5 104-105 0.0 2.7603644785257443E-4 5.5207289570514894E-5 3.233905004816836 5.5207289570514894E-5 106-107 0.0 2.7603644785257443E-4 5.5207289570514894E-5 3.5100518672485514 5.5207289570514894E-5 108-109 0.0 2.7603644785257443E-4 5.5207289570514894E-5 3.813526338017672 5.5207289570514894E-5 110-111 0.0 2.7603644785257443E-4 5.5207289570514894E-5 4.160973414929707 5.5207289570514894E-5 112-113 0.0 3.3124373742308933E-4 5.5207289570514894E-5 4.537404318866264 5.5207289570514894E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5420 0.0 40.00424 1 GTACATG 7900 0.0 34.98604 1 ATCAACG 6225 0.0 34.829067 3 CAACGCA 6340 0.0 34.573105 5 TCAACGC 6295 0.0 34.34715 4 AACGCAG 6470 0.0 33.297485 6 TACATGG 8045 0.0 33.094917 2 TATCAAC 6630 0.0 32.971016 2 ACATGGG 8125 0.0 31.596117 3 GGTATCA 3690 0.0 30.348368 1 GTATAGG 645 0.0 28.62898 1 ACGCAGA 7485 0.0 28.22251 7 TACCTGG 1610 0.0 27.376987 2 CGCAGAG 7380 0.0 27.167427 8 CATGGGA 5060 0.0 26.13258 4 CGCATAG 195 1.466813E-6 24.407896 9 GCAGAGT 8095 0.0 23.518417 9 AGTACAT 5830 0.0 21.557266 2 GTACCTG 2125 0.0 21.303871 1 ATGGGTA 995 0.0 20.951965 5 >>END_MODULE