FastQCFastQC Report
Thu 26 May 2016
SRR1364608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811355
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.3310781155543778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.294641304437838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.18593815127349417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51650.041.648231
GTATCAA80050.041.3878751
ATCAACG96700.033.9625473
TATCAAC98400.033.4034652
TCAACGC97950.033.288924
CAACGCA99050.032.4125985
AACGCAG100900.032.0464676
GTACATG78050.030.7674731
TACATGG79500.029.81012
ACATGGG78300.028.9501533
TACCTGG16250.027.8488832
TAGGCGG1107.704871E-427.0742765
ACGCAGA118300.027.0324297
GTTCGCG900.00948997426.4675037
CGCAGAG119550.026.4509018
GAGTACT70750.024.585112-13
GTACCTG18900.024.276641
GCAGAGT129000.023.3142369
AACGCCG2052.2483E-623.2384786
GTACTTT77200.022.91683814-15
Produced by FastQC (version 0.11.5)