##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364608_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1811355 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.462440548650044 31.0 30.0 33.0 26.0 34.0 2 29.6413265207538 31.0 30.0 34.0 26.0 34.0 3 29.65427594259546 31.0 30.0 34.0 25.0 34.0 4 33.30691554112805 35.0 33.0 37.0 28.0 37.0 5 32.695107253961815 35.0 33.0 37.0 26.0 37.0 6 32.673845822602416 35.0 33.0 37.0 25.0 37.0 7 32.46503253089538 35.0 33.0 37.0 24.0 37.0 8 32.390948212802016 35.0 33.0 37.0 23.0 37.0 9 33.688675052653956 38.0 33.0 39.0 23.0 39.0 10-11 33.47404015226171 37.0 33.0 39.0 20.5 39.0 12-13 33.32900176939363 37.0 33.0 39.0 18.0 39.0 14-15 34.29549701742618 38.0 33.0 41.0 18.0 41.0 16-17 34.210036961280366 38.0 33.0 41.0 17.5 41.0 18-19 34.2545409375854 38.5 33.0 41.0 18.0 41.0 20-21 34.28143958528284 39.0 33.0 41.0 18.0 41.0 22-23 34.27196132177293 39.0 33.0 41.0 18.0 41.0 24-25 34.25960151378388 39.0 33.0 41.0 18.0 41.0 26-27 34.160316172147375 39.0 33.0 41.0 18.0 41.0 28-29 33.92981911331572 38.5 33.0 41.0 17.0 41.0 30-31 33.85929649350901 38.0 33.0 41.0 17.0 41.0 32-33 33.681524880545226 38.0 33.0 40.5 16.0 41.0 34-35 33.5498488700448 38.0 33.0 40.0 16.0 41.0 36-37 33.37616590894662 38.0 32.0 40.0 14.5 41.0 38-39 33.24951044935973 38.0 32.0 40.0 10.5 41.0 40-41 33.07903282349402 38.0 32.0 40.0 9.0 41.0 42-43 32.90844285079402 38.0 32.0 40.0 8.0 41.0 44-45 32.71443228963952 38.0 31.5 40.0 4.5 41.0 46-47 32.46230142628033 38.0 31.0 40.0 2.0 41.0 48-49 32.20969467608503 38.0 31.0 40.0 2.0 41.0 50-51 32.012474363114904 38.0 30.0 40.0 2.0 41.0 52-53 31.85071037979855 37.5 30.0 40.0 2.0 41.0 54-55 31.642510717115087 37.0 30.0 40.0 2.0 41.0 56-57 31.424137179073128 37.0 30.0 40.0 2.0 41.0 58-59 31.238515641605318 36.5 29.5 40.0 2.0 41.0 60-61 31.02373085342189 36.0 29.0 40.0 2.0 41.0 62-63 30.75998492840995 36.0 29.0 39.5 2.0 41.0 64-65 30.493823132406405 35.5 29.0 39.0 2.0 41.0 66-67 30.223258831096057 35.0 28.5 39.0 2.0 41.0 68-69 29.909755404103556 35.0 28.0 38.5 2.0 40.0 70-71 29.64877702051779 35.0 28.0 38.0 2.0 40.0 72-73 29.48883487775726 35.0 28.0 37.0 2.0 40.0 74-75 29.204472618564555 35.0 28.0 37.0 2.0 39.0 76-77 28.908649602093462 34.0 28.0 36.5 2.0 39.0 78-79 28.604286293962254 34.0 28.0 36.0 2.0 39.0 80-81 28.328799986750248 34.0 27.5 36.0 2.0 37.5 82-83 28.068386925809683 34.0 27.0 35.0 2.0 37.0 84-85 28.04868178794328 34.0 28.0 35.0 2.0 37.0 86-87 27.904790612552482 34.0 27.5 35.0 2.0 36.0 88-89 27.75758948411548 34.0 27.0 35.0 2.0 36.0 90-91 27.599736661228746 34.0 27.0 35.0 2.0 36.0 92-93 27.46511948237645 34.0 27.0 35.0 2.0 35.5 94-95 27.37831402458381 34.0 27.0 35.0 2.0 35.0 96-97 27.295106977925364 34.0 26.5 35.0 2.0 35.0 98-99 27.201538903196777 34.0 26.0 35.0 2.0 35.0 100-101 27.105997167866043 34.0 26.0 35.0 2.0 35.0 102-103 25.936133999133247 32.0 24.0 34.0 2.0 35.0 104-105 26.85308263703139 33.5 25.0 35.0 2.0 35.0 106-107 27.02095144242846 34.0 26.0 35.0 2.0 35.0 108-109 27.034554794615083 34.0 26.0 35.0 2.0 35.0 110-111 26.97914379014605 34.0 25.5 35.0 2.0 35.0 112-113 26.928683499369257 34.0 25.0 35.0 2.0 35.0 114-115 26.84923965760439 34.0 25.0 35.0 2.0 35.0 116-117 26.73722075462844 34.0 25.0 35.0 2.0 35.0 118-119 26.63413577128724 34.0 25.0 35.0 2.0 35.0 120-121 26.498121019899468 34.0 25.0 35.0 2.0 35.0 122-123 26.31540449000886 34.0 25.0 35.0 2.0 35.0 124-125 25.52020918042018 33.0 21.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8735165231316131 1101 2 0.9152826027437264 1101 3 0.6886300917331845 1101 4 0.7786497020643068 1101 5 1.131930891088544 1101 6 1.1052533862594132 1101 7 1.155894427087155 1101 8 1.1918526570105996 1101 9 1.279645429945056 1101 10-11 1.3067654068054821 1101 12-13 1.3060099225948818 1101 14-15 1.4009911165434872 1101 16-17 1.5396318269583134 1101 18-19 1.4560497398447296 1101 20-21 1.4871941960794288 1101 22-23 1.347799399840163 1101 24-25 1.3694022965990271 1101 26-27 1.3562409188319364 1101 28-29 1.5030561321523805 1101 30-31 1.433618615585523 1101 32-33 1.5186843966108299 1101 34-35 1.426285140914274 1101 36-37 1.4128523017390577 1101 38-39 1.4543865998161039 1101 40-41 1.526633959815122 1101 42-43 1.5318361627292276 1101 44-45 1.4969983203167985 1101 46-47 1.4547802254307172 1101 48-49 1.5957155408438908 1101 50-51 1.5963803813641917 1101 52-53 1.6143084384480773 1101 54-55 1.5212702066979915 1101 56-57 1.7203452608484824 1101 58-59 1.6997071426044705 1101 60-61 1.6651605814363855 1101 62-63 1.645010483041336 1101 64-65 1.6062161543244855 1101 66-67 1.574724602474003 1101 68-69 1.6427223504537736 1101 70-71 1.560780172746238 1101 72-73 1.6282932714359575 1101 74-75 1.6209404589251442 1101 76-77 1.6177542610748432 1101 78-79 1.5292542357289634 1101 80-81 1.4630750861541415 1101 82-83 1.4370806358244614 1101 84-85 1.6041123032694706 1101 86-87 1.5488084564252595 1101 88-89 1.559712541026947 1101 90-91 1.6003852805233159 1101 92-93 1.4979745634709687 1101 94-95 1.4206031048392624 1101 96-97 1.5429462797834859 1101 98-99 1.5929004567836031 1101 100-101 1.6206514136679715 1101 102-103 1.580448796733691 1101 104-105 1.5368620369393824 1101 106-107 1.5899606517336338 1101 108-109 1.673803195207686 1101 110-111 1.6303164879665637 1101 112-113 1.6024615243071736 1101 114-115 1.5413807108245727 1101 116-117 1.574451713823219 1101 118-119 1.6121553929580692 1101 120-121 1.6239998785493022 1101 122-123 1.690425171342934 1101 124-125 1.595071571528564 1102 1 0.3952349797045436 1102 2 -0.5204556043932946 1102 3 0.12904563231436939 1102 4 0.4109001704538926 1102 5 0.7393482352293859 1102 6 0.6255497212818284 1102 7 0.582584211545921 1102 8 0.5196991149135073 1102 9 0.7692776962725247 1102 10-11 0.8255271028734157 1102 12-13 1.0216273109223835 1102 14-15 1.0625168908218185 1102 16-17 1.1053046673624252 1102 18-19 1.1801290385017396 1102 20-21 1.0982699280851236 1102 22-23 1.1580966973443836 1102 24-25 1.16788595026744 1102 26-27 1.1291754656944093 1102 28-29 1.2345532397851926 1102 30-31 1.0972418892705065 1102 32-33 1.0730758292103033 1102 34-35 0.9623806694206962 1102 36-37 0.9203730579872627 1102 38-39 0.8733434445382642 1102 40-41 0.869398065368749 1102 42-43 0.6834471439903638 1102 44-45 0.5600419721477508 1102 46-47 0.5522799055148937 1102 48-49 0.6174629812438468 1102 50-51 0.6251040375263202 1102 52-53 0.6897999604052387 1102 54-55 0.7050198772120382 1102 56-57 0.44301720330081906 1102 58-59 0.4519837401875435 1102 60-61 0.38918492019497464 1102 62-63 0.6865788963572719 1102 64-65 0.5448314164421859 1102 66-67 0.39983714722120567 1102 68-69 0.2500083103598456 1102 70-71 0.14784731569556797 1102 72-73 0.2101926800565117 1102 74-75 0.25569638775836623 1102 76-77 0.37424937265164004 1102 78-79 0.4122573266080245 1102 80-81 0.26034688773414416 1102 82-83 0.22651778084093976 1102 84-85 0.37986949207886767 1102 86-87 0.2528204196365813 1102 88-89 0.36404491224305247 1102 90-91 0.3249251759409191 1102 92-93 0.5049949385545034 1102 94-95 0.424711425603828 1102 96-97 0.3394562988909371 1102 98-99 0.38846262271765397 1102 100-101 0.32919086940638564 1102 102-103 0.33865368067259993 1102 104-105 0.31193578163890123 1102 106-107 0.4425908793496447 1102 108-109 0.4630673942751713 1102 110-111 0.4256257289949765 1102 112-113 0.5216182480628646 1102 114-115 0.6194280047741678 1102 116-117 0.5778692078049694 1102 118-119 0.4898381742679838 1102 120-121 0.4485726678762525 1102 122-123 0.4275512936459336 1102 124-125 0.4466619359919868 1103 1 0.38192573049012424 1103 2 -0.9225745875841334 1103 3 -0.02153363322945978 1103 4 0.3896919721322476 1103 5 0.6760634189102106 1103 6 0.6637930462037147 1103 7 0.6866633362805814 1103 8 0.7713520921091046 1103 9 0.8436577741683777 1103 10-11 1.030282835142316 1103 12-13 1.0421400900501254 1103 14-15 1.1339178529992537 1103 16-17 1.3169119066233606 1103 18-19 1.248411188908193 1103 20-21 1.197255016783771 1103 22-23 1.0908533624044523 1103 24-25 0.9021572043818011 1103 26-27 1.0112814588220331 1103 28-29 1.2268841491230091 1103 30-31 1.2020834984354565 1103 32-33 1.1795224124452162 1103 34-35 1.105272895307543 1103 36-37 1.254936338072838 1103 38-39 1.3293386314248608 1103 40-41 1.2956640121462257 1103 42-43 1.213943738412091 1103 44-45 1.3232656505729992 1103 46-47 1.3568593202162589 1103 48-49 1.1913307475206594 1103 50-51 1.296633675908332 1103 52-53 1.200085569262967 1103 54-55 1.2602977721761874 1103 56-57 1.4135043251057553 1103 58-59 1.3650415921631556 1103 60-61 1.157123062115037 1103 62-63 1.3045286981669797 1103 64-65 1.2633362178678667 1103 66-67 1.316986952592881 1103 68-69 1.2187801204756212 1103 70-71 1.2367861154671544 1103 72-73 1.2226626398396334 1103 74-75 1.2030422462434807 1103 76-77 1.186904582757844 1103 78-79 1.221591137839141 1103 80-81 1.1707315917399121 1103 82-83 1.1458374666305673 1103 84-85 1.225970775612879 1103 86-87 1.1207647950059965 1103 88-89 1.075452068910785 1103 90-91 1.0858299555495243 1103 92-93 1.1360525155904746 1103 94-95 1.0718375271712048 1103 96-97 1.1027473208315648 1103 98-99 1.1435103253019783 1103 100-101 1.1716160874936286 1103 102-103 1.09884105704716 1103 104-105 1.135412426796453 1103 106-107 1.0437477964191686 1103 108-109 1.0616335339556429 1103 110-111 1.1438060571192992 1103 112-113 1.0583772328565892 1103 114-115 1.0586954242040534 1103 116-117 1.1034846496173891 1103 118-119 1.108526945933395 1103 120-121 1.1313165321028258 1103 122-123 1.157150382671837 1103 124-125 1.0665279669210257 1104 1 0.16052775312163803 1104 2 -0.8458435644602496 1104 3 -0.0744708497364357 1104 4 0.0715370896252665 1104 5 0.5298420187542234 1104 6 0.5873016713632992 1104 7 0.6901110036313369 1104 8 0.6029615917670554 1104 9 0.6692041722546804 1104 10-11 0.8145590755400391 1104 12-13 0.6923897599890765 1104 14-15 0.7520211566597723 1104 16-17 0.8734604593655888 1104 18-19 0.760940436891886 1104 20-21 0.8005328061808541 1104 22-23 0.7136761032836603 1104 24-25 0.8042045087743332 1104 26-27 0.6446765015883571 1104 28-29 1.0378517617468077 1104 30-31 0.9121717107899769 1104 32-33 0.9649277230511828 1104 34-35 0.8926441388206996 1104 36-37 0.8646252637077936 1104 38-39 0.8747734950214152 1104 40-41 1.0454812803300868 1104 42-43 0.9447884877182062 1104 44-45 0.9091280902796726 1104 46-47 0.8694536544951177 1104 48-49 0.9860850644576971 1104 50-51 0.9733514094455913 1104 52-53 0.696324452850547 1104 54-55 0.9243138743622126 1104 56-57 0.8271029490888893 1104 58-59 0.9237110322140474 1104 60-61 0.9230907183112507 1104 62-63 0.7664684658388587 1104 64-65 0.8307980266459651 1104 66-67 0.9118838602585733 1104 68-69 0.747722007945498 1104 70-71 0.8226676181054664 1104 72-73 0.8466000175645405 1104 74-75 0.9044995801345408 1104 76-77 0.9081718428808045 1104 78-79 0.8964442443344822 1104 80-81 0.8091810346665866 1104 82-83 0.8676806996166313 1104 84-85 0.889661718650661 1104 86-87 0.769507676362668 1104 88-89 0.7241007249196372 1104 90-91 0.8329604396826298 1104 92-93 0.7835745523394486 1104 94-95 0.7743386927333091 1104 96-97 0.7507527390613937 1104 98-99 0.7606139143618122 1104 100-101 0.8793361390833709 1104 102-103 0.94630329065631 1104 104-105 0.8498573360133292 1104 106-107 0.8332900355184378 1104 108-109 0.7858727051211574 1104 110-111 0.8274289291514698 1104 112-113 0.8271977929364134 1104 114-115 0.9323520321786773 1104 116-117 0.8979778043909654 1104 118-119 0.8644266509481433 1104 120-121 0.800728260925542 1104 122-123 0.8647832894312231 1104 124-125 0.7826558606740477 1105 1 0.4945688067405456 1105 2 0.17424958374514077 1105 3 0.4832345113044809 1105 4 0.4246499435252389 1105 5 0.5335428780036651 1105 6 0.4034691363065832 1105 7 0.5184201868397977 1105 8 0.4340656725911032 1105 9 0.5816705920557652 1105 10-11 0.6290444765850793 1105 12-13 0.6407105110383071 1105 14-15 0.618472679015234 1105 16-17 0.7904730857328275 1105 18-19 0.738920111908854 1105 20-21 0.8197042582582341 1105 22-23 0.5902298347886585 1105 24-25 0.5554978576376826 1105 26-27 0.615300864905663 1105 28-29 0.7201095195827207 1105 30-31 0.6894247841283914 1105 32-33 0.7002124523328987 1105 34-35 0.724214744076896 1105 36-37 0.7398003348516085 1105 38-39 0.7235261678937093 1105 40-41 0.6518762700422442 1105 42-43 0.8388101633448954 1105 44-45 0.842194946344506 1105 46-47 0.8888410077327791 1105 48-49 0.8791040478261323 1105 50-51 0.8928108492391758 1105 52-53 0.7587990917671448 1105 54-55 0.827592079870378 1105 56-57 0.8554275613962616 1105 58-59 0.7787249715152846 1105 60-61 0.9037917305238743 1105 62-63 0.8663638480503018 1105 64-65 0.8256330627569604 1105 66-67 0.831784264230091 1105 68-69 0.8041994145397027 1105 70-71 0.7944323007121703 1105 72-73 0.6953988316749715 1105 74-75 0.7507833316203545 1105 76-77 0.7508944866369873 1105 78-79 0.7579984219821014 1105 80-81 0.606117561849949 1105 82-83 0.7611219914969389 1105 84-85 0.5102846744942262 1105 86-87 0.679142351947938 1105 88-89 0.7250582717938236 1105 90-91 0.6463298866676084 1105 92-93 0.42728439286470987 1105 94-95 0.5292230249480987 1105 96-97 0.572405036217873 1105 98-99 0.585746588678763 1105 100-101 0.6906778280306085 1105 102-103 0.2188818250353144 1105 104-105 0.24084517544022788 1105 106-107 0.5646300540864715 1105 108-109 0.5966468236442566 1105 110-111 0.6447495930966696 1105 112-113 0.5827608992787532 1105 114-115 0.6206880184672308 1105 116-117 0.3973192792959743 1105 118-119 0.1845982458973836 1105 120-121 0.2791089331774863 1105 122-123 0.3723944028438666 1105 124-125 0.16259211008052077 1106 1 0.18469664639003724 1106 2 0.144064086838128 1106 3 0.34417133378608256 1106 4 0.22743395216971862 1106 5 0.31721892399889384 1106 6 0.3961455439448329 1106 7 0.4192567465583181 1106 8 0.5115864362207674 1106 9 0.5860642848165014 1106 10-11 0.5797094422187143 1106 12-13 0.42908226155243767 1106 14-15 0.6192710824397167 1106 16-17 0.7211597165333501 1106 18-19 0.6766817215621188 1106 20-21 0.7443999396443104 1106 22-23 0.6782869533086071 1106 24-25 0.7423709101437765 1106 26-27 0.654316028980297 1106 28-29 0.7722402832200927 1106 30-31 0.6765096047089898 1106 32-33 0.6121424179909454 1106 34-35 0.44547421278808486 1106 36-37 0.4254197815300813 1106 38-39 0.5534302540999505 1106 40-41 0.5796947530076224 1106 42-43 0.6015053239855987 1106 44-45 0.6884280372202269 1106 46-47 0.6250292389385947 1106 48-49 0.7041009570249486 1106 50-51 0.7814033382195973 1106 52-53 0.7273148800422433 1106 54-55 0.7950316139035039 1106 56-57 0.5839067175728374 1106 58-59 0.6992210187596903 1106 60-61 0.8616736205365889 1106 62-63 0.712039502329862 1106 64-65 0.6796374302325319 1106 66-67 0.631483640440873 1106 68-69 0.7462691897941198 1106 70-71 0.7996518992447257 1106 72-73 0.8610555581708113 1106 74-75 0.8560682289504946 1106 76-77 0.874468830493953 1106 78-79 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-2.1225359208017096 2216 70-71 -2.7061352062560786 2216 72-73 -2.9480715057653697 2216 74-75 -2.670038690534799 2216 76-77 -2.9601568660290525 2216 78-79 -2.605765089259613 2216 80-81 -2.2968720708273125 2216 82-83 -2.2334699058365253 2216 84-85 -2.2154303420087373 2216 86-87 -2.1881569490386603 2216 88-89 -2.0772232454840527 2216 90-91 -2.0213040121500256 2216 92-93 -2.1839582260630657 2216 94-95 -1.995459238295556 2216 96-97 -2.0794479038760727 2216 98-99 -2.0954427562162614 2216 100-101 -2.231585343502722 2216 102-103 -2.0695200808132412 2216 104-105 -1.860269234660901 2216 106-107 -2.087567132949104 2216 108-109 -2.2382899075355027 2216 110-111 -2.0791546608679248 2216 112-113 -2.1909515413095804 2216 114-115 -2.2851354187250195 2216 116-117 -2.4497696735666246 2216 118-119 -2.446203717050029 2216 120-121 -2.402386298470798 2216 122-123 -2.427718116230537 2216 124-125 -2.614402333722861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76305.0 3 23701.0 4 10964.0 5 8157.0 6 8818.0 7 10293.0 8 11523.0 9 12491.0 10 13099.0 11 14383.0 12 16082.0 13 17680.0 14 19160.0 15 16283.0 16 11014.0 17 9499.0 18 8747.0 19 8121.0 20 7991.0 21 8239.0 22 9955.0 23 13180.0 24 17546.0 25 23452.0 26 28101.0 27 31514.0 28 36387.0 29 42444.0 30 49302.0 31 58371.0 32 70984.0 33 88361.0 34 112432.0 35 152217.0 36 239296.0 37 375040.0 38 149533.0 39 690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3389417791836 19.490942115049556 13.660795273128626 24.509320832638217 2 15.475244669319347 20.76487546367557 39.186593988709355 24.573285878295728 3 19.640655752185516 24.97550176525308 29.181855572209752 26.201986910351643 4 12.789047739461996 17.0717190645349 36.51638742362823 33.62284577237487 5 12.962675600946074 38.85798571170603 34.00789572648912 14.171442960858776 6 31.796871722054515 37.40788628137718 16.87455972016421 13.920682276404092 7 28.223583021319964 32.96710637456842 20.269312348662936 18.539998255448687 8 26.008278889714543 36.6292839500175 18.768777389731657 18.593659770536295 9 25.95517788507722 16.58718582616828 19.67081792575433 37.786818363000165 10-11 24.900596915659563 27.448591564078118 26.73774488953801 20.91306663072431 12-13 25.86912141653312 24.437653301089206 27.685448595047713 22.007776687329965 14-15 22.542431882484053 25.725440744158725 25.922793201181634 25.809334172175596 16-17 21.92116138222912 29.063057803147323 26.50088143771979 22.514899376903763 18-19 21.745797333480553 27.39053744444751 29.29320672426643 21.570458497805504 20-21 22.474645623990817 26.482040817115283 29.554629269980037 21.488684288913866 22-23 22.09316225418323 26.44734876918036 29.424936524452544 22.03455245218387 24-25 21.950473540526293 26.528400009937315 29.60181742397266 21.919309025563734 26-27 21.75327310218041 26.859947387453037 29.453999906147608 21.93277960421894 28-29 21.61883822581888 26.813744565221892 29.638600201662964 21.928817007296267 30-31 21.836001225601827 26.60389597842499 29.515887277756153 22.044215518217026 32-33 21.655726029514877 26.668313743243765 29.543557354150906 22.13240287309045 34-35 22.117199555029245 26.642596288413923 29.424325988003456 21.815878168553375 36-37 22.08304740494802 26.384919322649225 29.370232494971987 22.161800777430766 38-39 21.847208305384644 26.75058174681385 29.47127978778318 21.930930160018328 40-41 22.06838089075907 26.536585338923256 29.426605732507284 21.96842803781039 42-43 21.920037763089066 26.55380066996357 29.17828015077631 22.34788141617105 44-45 22.032511572828074 26.80623069470093 28.978554728366333 22.182703004104663 46-47 22.21675669602869 27.11764986790265 28.372788635680447 22.292804800388218 48-49 22.300291550018137 27.07611382209873 28.292635394646986 22.33095923323615 50-51 22.29136958145559 27.256978086497963 28.111700174842063 22.339952157204383 52-53 22.430243461588255 27.26651644750735 27.989649728780165 22.313590362124238 54-55 22.261373391742644 27.24112611829266 28.075998354822772 22.421502135141925 56-57 22.255117110402605 27.49219494016811 27.933915779884895 22.318772169544392 58-59 22.33090697295671 27.07371001267007 28.073845270529517 22.521537743843698 60-61 22.315090084494756 27.197236323084102 28.254897576676026 22.232776015745117 62-63 22.224329300440832 27.16126877392891 28.264448437771723 22.34995348785854 64-65 22.10771844640094 27.038205088214955 28.26870125335214 22.58537521203196 66-67 22.09522705378018 27.39090349489747 28.018499962735078 22.495369488587272 68-69 22.215054422371487 27.106760376218396 28.21869868271665 22.459486518693467 70-71 22.145576101868492 27.02418907392532 28.13275144850126 22.697483375704927 72-73 22.304529066369867 27.096664488686788 27.991982604869452 22.606823840073893 74-75 22.14349086536524 27.344858065481397 27.89297479756174 22.61867627159162 76-77 22.314594136817167 27.154223648056657 28.051765077398628 22.479417137727545 78-79 22.341063359345455 27.171892554386684 27.982868988894932 22.504175097372933 80-81 22.15363517339669 27.37064914135989 27.93023995989673 22.545475725346687 82-83 22.379956148692536 27.396632950533817 27.839755850406476 22.38365505036717 84-85 22.211358639469676 27.266614311681465 27.877262984427382 22.644764064421473 86-87 22.183359175066723 27.232807196193548 28.054764784098456 22.529068844641266 88-89 22.34004377938063 27.215951594248505 27.871400139674442 22.572604486696424 90-91 22.18201291298503 27.423310173875358 28.001716946705642 22.392959966433967 92-93 22.208373327193808 27.251009059937925 27.998600939534807 22.54201667333346 94-95 22.145120389832165 27.15232263403931 27.98570151625322 22.7168554598753 96-97 22.280521786467716 27.138218644806123 27.88668419794406 22.6945753707821 98-99 22.024830208762346 27.106946120611124 27.81918047502901 23.04904319559752 100-101 21.939326318474308 27.128893963078827 28.05006002444039 22.88171969400647 102-103 22.177630055298398 26.898848348018017 28.02201336071984 22.90150823596375 104-105 22.092444539156116 26.84286349567214 28.127543861731514 22.937148103440233 106-107 21.989584149975876 27.068235798149153 27.963460054171698 22.97871999770328 108-109 22.059364669671016 26.98761223179964 27.698000703928887 23.255022394600452 110-111 21.912091934376594 27.029393505510164 27.946341249315765 23.112173310797477 112-113 22.099771295911623 26.956772580905625 27.94993078147235 22.99352534171041 114-115 21.827643908414917 27.036265356699424 27.882985166255093 23.25310556863057 116-117 21.741569218725243 27.02321911943241 27.938441204675733 23.296770457166613 118-119 21.728091142547424 27.08466466241543 27.770464189996613 23.416780005040533 120-121 21.640904933503727 27.144526594506758 27.850113452116414 23.364455019873102 122-123 21.733336020111082 27.090089958287777 27.770056358843824 23.406517662757313 124-125 21.59846167138653 26.96337166916991 27.807481464448646 23.63068519499491 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 166.0 1 127.5 2 189.0 3 392.0 4 340.5 5 265.0 6 693.5 7 1560.5 8 2389.5 9 2757.0 10 2277.0 11 1575.0 12 1399.0 13 1470.0 14 1598.0 15 1755.5 16 1956.0 17 2121.0 18 2304.0 19 2586.5 20 2893.5 21 3291.0 22 3926.5 23 4709.0 24 5752.5 25 7227.0 26 9493.5 27 12241.0 28 14596.0 29 17290.0 30 20690.5 31 24694.5 32 29177.0 33 33149.0 34 37305.5 35 40865.0 36 44942.5 37 49988.0 38 53260.5 39 55208.0 40 56775.5 41 58659.0 42 60623.0 43 62393.0 44 64273.5 45 66236.0 46 67031.0 47 68013.5 48 71132.5 49 71270.5 50 68519.0 51 66045.0 52 62995.0 53 60688.5 54 58535.5 55 53892.5 56 48540.5 57 43973.0 58 39723.5 59 35235.0 60 29611.5 61 23863.5 62 18762.0 63 14040.0 64 10413.0 65 8038.5 66 5883.5 67 4524.5 68 3563.0 69 2586.0 70 1872.0 71 1305.5 72 1002.5 73 849.0 74 637.5 75 485.5 76 331.0 77 187.5 78 100.5 79 63.0 80 44.0 81 25.5 82 16.5 83 11.0 84 10.5 85 9.0 86 7.5 87 5.5 88 2.0 89 1.0 90 1.5 91 1.5 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061224884133701016 2 0.07513712110547077 3 0.0 4 0.008225886146006719 5 0.027714059364398476 6 3.8645102699360424E-4 7 5.5207289570514894E-5 8 5.5207289570514894E-5 9 9.385239226987531E-4 10-11 0.057912446759470125 12-13 0.009523257450913819 14-15 0.007094136709811163 16-17 0.053026601632479554 18-19 2.7603644785257443E-4 20-21 0.009854501188336908 22-23 0.012394036508580595 24-25 0.0 26-27 0.0 28-29 0.05015582257481278 30-31 0.0 32-33 0.0035884738220834677 34-35 0.0 36-37 2.2082915828205957E-4 38-39 0.0 40-41 5.5207289570514894E-5 42-43 0.0034504555981571803 44-45 0.0 46-47 8.281093435577235E-5 48-49 1.1041457914102979E-4 50-51 3.312437374230894E-4 52-53 8.281093435577235E-5 54-55 0.0 56-57 0.0016562186871154467 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.900911196314361E-4 66-67 0.0 68-69 7.45298409201951E-4 70-71 0.0 72-73 0.0024015170963173978 74-75 0.0034228519533719233 76-77 9.93731212269268E-4 78-79 0.0011041457914102977 80-81 0.0017390296214712191 82-83 3.588473822083468E-4 84-85 1.3801822392628721E-4 86-87 4.692619613493766E-4 88-89 0.0 90-91 0.0 92-93 0.0077014168950868276 94-95 0.006293631011038698 96-97 0.006404045590179728 98-99 7.176947644166936E-4 100-101 5.244692509198915E-4 102-103 2.7603644785257447E-5 104-105 5.244692509198915E-4 106-107 0.004250961296929647 108-109 0.005106674285272627 110-111 0.0010765421466250402 112-113 0.0034504555981571803 114-115 6.624874748461788E-4 116-117 0.0025395353202436848 118-119 0.0021254806484648234 120-121 0.013166938562567801 122-123 8.557129883429808E-4 124-125 0.0021254806484648234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1811355.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.77252986994232 #Duplication Level Percentage of deduplicated Percentage of total 1 82.10560394039118 52.3608207977825 2 10.70043150690975 13.647871757913476 3 3.106148110250624 5.9426076942426835 4 1.3710785988770917 3.4974860360371194 5 0.7008187236515576 2.2346491493741905 6 0.4325821675166011 1.65521155194941 7 0.2944721400337416 1.3145463342317338 8 0.1986289105652825 1.0133654505646863 9 0.15631160376545158 0.8971547778135764 >10 0.8397428112580407 9.963317953473934 >50 0.06321090668691495 2.757674337490583 >100 0.029749481903631456 3.4449708518651754 >500 7.849916936721779E-4 0.28239913437968894 >1k 2.616638978907259E-4 0.35749049372823716 >5k 1.7444259859381728E-4 0.6304336791529236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5997 0.3310781155543778 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5337 0.294641304437838 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3368 0.18593815127349417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 5.5207289570514894E-5 0.0 0.0 0.0 0.0 10-11 5.5207289570514894E-5 0.0 0.0 5.5207289570514894E-5 0.0 12-13 5.5207289570514894E-5 0.0 0.0 8.281093435577233E-5 0.0 14-15 5.5207289570514894E-5 2.7603644785257447E-5 0.0 1.1041457914102979E-4 0.0 16-17 5.5207289570514894E-5 5.5207289570514894E-5 0.0 1.1041457914102979E-4 0.0 18-19 5.5207289570514894E-5 5.5207289570514894E-5 0.0 1.6562186871154467E-4 0.0 20-21 5.5207289570514894E-5 5.5207289570514894E-5 0.0 2.760364478525745E-4 2.7603644785257447E-5 22-23 5.5207289570514894E-5 5.5207289570514894E-5 0.0 3.3124373742308933E-4 5.5207289570514894E-5 24-25 5.5207289570514894E-5 5.5207289570514894E-5 0.0 3.3124373742308933E-4 5.5207289570514894E-5 26-27 5.5207289570514894E-5 5.5207289570514894E-5 0.0 5.244692509198915E-4 5.5207289570514894E-5 28-29 5.5207289570514894E-5 5.5207289570514894E-5 0.0 0.0010213348570545255 5.5207289570514894E-5 30-31 5.5207289570514894E-5 8.281093435577233E-5 0.0 0.0024291207411026553 5.5207289570514894E-5 32-33 5.5207289570514894E-5 1.1041457914102979E-4 0.0 0.004692619613493766 5.5207289570514894E-5 34-35 5.5207289570514894E-5 1.1041457914102979E-4 0.0 0.007977453342939402 5.5207289570514894E-5 36-37 5.5207289570514894E-5 1.1041457914102979E-4 0.0 0.012890902114715228 5.5207289570514894E-5 38-39 5.5207289570514894E-5 1.1041457914102979E-4 0.0 0.022220934052132244 5.5207289570514894E-5 40-41 5.5207289570514894E-5 1.1041457914102979E-4 0.0 0.03524985439077376 5.5207289570514894E-5 42-43 5.5207289570514894E-5 1.3801822392628724E-4 0.0 0.048582414822053104 5.5207289570514894E-5 44-45 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.06288110282081646 5.5207289570514894E-5 46-47 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.08203803230178514 5.5207289570514894E-5 48-49 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.10155380916496215 5.5207289570514894E-5 50-51 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.1243544197575848 5.5207289570514894E-5 52-53 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.1476794996011273 5.5207289570514894E-5 54-55 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.17186029243301287 5.5207289570514894E-5 56-57 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.20236231992072234 5.5207289570514894E-5 58-59 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.2361215774930922 5.5207289570514894E-5 60-61 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.2763676915899975 5.5207289570514894E-5 62-63 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.3153716416715663 5.5207289570514894E-5 64-65 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.3565010724015999 5.5207289570514894E-5 66-67 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.40458662161751835 5.5207289570514894E-5 68-69 5.5207289570514894E-5 1.6562186871154467E-4 0.0 0.4634375922996873 5.5207289570514894E-5 70-71 5.5207289570514894E-5 2.2082915828205957E-4 0.0 0.5316738022088436 5.5207289570514894E-5 72-73 5.5207289570514894E-5 2.2082915828205957E-4 0.0 0.6015662308051155 5.5207289570514894E-5 74-75 5.5207289570514894E-5 2.2082915828205957E-4 0.0 0.6718175067835958 5.5207289570514894E-5 76-77 5.5207289570514894E-5 2.2082915828205957E-4 0.0 0.7480035663909063 5.5207289570514894E-5 78-79 5.5207289570514894E-5 2.2082915828205957E-4 0.0 0.8438434210853201 5.5207289570514894E-5 80-81 5.5207289570514894E-5 2.2082915828205957E-4 0.0 0.9533470799484364 5.5207289570514894E-5 82-83 5.5207289570514894E-5 2.2082915828205957E-4 0.0 1.0725948254207487 5.5207289570514894E-5 84-85 5.5207289570514894E-5 2.2082915828205957E-4 0.0 1.1849692633415314 5.5207289570514894E-5 86-87 5.5207289570514894E-5 2.2082915828205957E-4 0.0 1.3110627127205876 5.5207289570514894E-5 88-89 5.5207289570514894E-5 2.2082915828205957E-4 0.0 1.457582859240734 5.5207289570514894E-5 90-91 5.5207289570514894E-5 2.2082915828205957E-4 0.0 1.6278145366314167 5.5207289570514894E-5 92-93 5.5207289570514894E-5 2.2082915828205957E-4 0.0 1.8109095124920294 5.5207289570514894E-5 94-95 5.5207289570514894E-5 2.2082915828205957E-4 0.0 1.9968476637655237 5.5207289570514894E-5 96-97 5.5207289570514894E-5 2.2082915828205957E-4 0.0 2.1890794460500564 5.5207289570514894E-5 98-99 5.5207289570514894E-5 2.2082915828205957E-4 0.0 2.404111838927212 5.5207289570514894E-5 100-101 5.5207289570514894E-5 2.2082915828205957E-4 0.0 2.6625371614067923 5.5207289570514894E-5 102-103 5.5207289570514894E-5 2.48432803067317E-4 0.0 2.9442599600851294 5.5207289570514894E-5 104-105 5.5207289570514894E-5 2.7603644785257443E-4 0.0 3.22253230316531 5.5207289570514894E-5 106-107 5.5207289570514894E-5 2.7603644785257443E-4 0.0 3.4976026234504003 5.5207289570514894E-5 108-109 5.5207289570514894E-5 2.7603644785257443E-4 0.0 3.8007458504820977 5.5207289570514894E-5 110-111 5.5207289570514894E-5 2.7603644785257443E-4 0.0 4.149545505988611 5.5207289570514894E-5 112-113 5.5207289570514894E-5 2.7603644785257443E-4 0.0 4.527715439546638 5.5207289570514894E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5165 0.0 41.64823 1 GTATCAA 8005 0.0 41.387875 1 ATCAACG 9670 0.0 33.962547 3 TATCAAC 9840 0.0 33.403465 2 TCAACGC 9795 0.0 33.28892 4 CAACGCA 9905 0.0 32.412598 5 AACGCAG 10090 0.0 32.046467 6 GTACATG 7805 0.0 30.767473 1 TACATGG 7950 0.0 29.8101 2 ACATGGG 7830 0.0 28.950153 3 TACCTGG 1625 0.0 27.848883 2 TAGGCGG 110 7.704871E-4 27.074276 5 ACGCAGA 11830 0.0 27.032429 7 GTTCGCG 90 0.009489974 26.467503 7 CGCAGAG 11955 0.0 26.450901 8 GAGTACT 7075 0.0 24.5851 12-13 GTACCTG 1890 0.0 24.27664 1 GCAGAGT 12900 0.0 23.314236 9 AACGCCG 205 2.2483E-6 23.238478 6 GTACTTT 7720 0.0 22.916838 14-15 >>END_MODULE