FastQCFastQC Report
Thu 26 May 2016
SRR1364607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769861
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39250.042.0431331
ATCAACG42850.036.2854353
CAACGCA45000.034.9489445
TCAACGC45350.034.679224
AACGCAG45300.033.773036
GGTATCA27950.033.4636081
GTACATG62750.033.4182661
TATCAAC51100.031.826172
TACATGG64900.030.6580452
ACATGGG64400.028.2134783
ACGCAGA53850.027.9582527
TACCTGG18850.026.8627342
CGCAGAG52700.026.6427048
GTACCTG20950.025.8769041
CGCATAG1901.1697539E-625.0503188
GTGTAGC10450.023.9435181
CATGGGA35350.021.907694
GCAGAGT59650.021.6428229
CATGGGG33050.021.6297974
GTATAGG6400.021.4093171
Produced by FastQC (version 0.11.5)