##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1769861 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.80361452114036 31.0 27.0 31.0 10.0 34.0 2 26.60052060585549 31.0 26.0 31.0 10.0 34.0 3 27.350728108026562 31.0 27.0 31.0 12.0 34.0 4 30.737610467714696 35.0 31.0 37.0 10.0 37.0 5 31.193450785118152 35.0 32.0 37.0 16.0 37.0 6 31.243968311635772 35.0 32.0 37.0 11.0 37.0 7 30.96814665106469 35.0 32.0 37.0 10.0 37.0 8 30.81597481384131 35.0 32.0 37.0 10.0 37.0 9 31.858054389582005 37.0 31.0 39.0 10.0 39.0 10-11 31.824809970952522 37.0 31.0 39.0 8.0 39.0 12-13 31.827042349653446 37.0 31.0 39.0 8.0 39.0 14-15 32.53595960360729 38.0 31.5 40.0 8.0 41.0 16-17 32.954988838106495 38.0 32.0 40.0 8.0 41.0 18-19 32.78023669655414 38.0 31.5 40.0 8.0 41.0 20-21 32.68993016965739 38.0 31.0 40.0 8.0 41.0 22-23 32.76189062304893 38.0 31.0 40.0 8.0 41.0 24-25 32.85951580378347 38.0 31.5 40.0 8.0 41.0 26-27 32.668706469039094 38.0 31.0 40.0 5.0 41.0 28-29 32.578506447681484 38.0 31.0 40.0 2.0 41.0 30-31 32.461686256717336 38.0 30.5 40.0 2.0 41.0 32-33 32.362163186826535 38.0 30.0 40.0 2.0 41.0 34-35 32.43374988205288 38.0 30.0 40.0 2.0 41.0 36-37 32.38876838350582 38.0 30.0 40.0 2.0 41.0 38-39 32.114278465936025 38.0 30.0 40.0 2.0 41.0 40-41 31.97992582468341 38.0 30.0 40.0 2.0 41.0 42-43 31.51401465990832 37.5 30.0 40.0 2.0 41.0 44-45 31.416183248288988 37.0 30.0 40.0 2.0 41.0 46-47 31.39679387251315 37.0 30.0 40.0 2.0 41.0 48-49 31.348790950249764 37.0 30.0 40.0 2.0 41.0 50-51 31.122488432707428 37.0 29.0 40.0 2.0 41.0 52-53 31.036057916412645 37.0 29.0 40.0 2.0 41.0 54-55 30.871374927183545 37.0 29.0 40.0 2.0 41.0 56-57 30.660538030952715 36.5 28.5 40.0 2.0 41.0 58-59 30.464672084417927 36.0 28.0 40.0 2.0 41.0 60-61 30.175256700949962 36.0 28.0 40.0 2.0 41.0 62-63 30.006068838174297 35.5 28.0 39.0 2.0 41.0 64-65 29.785234546667787 35.0 28.0 39.0 2.0 40.5 66-67 29.51734825503246 35.0 28.0 39.0 2.0 40.0 68-69 29.27094161631902 35.0 28.0 38.5 2.0 40.0 70-71 28.821125783324227 34.0 26.0 38.0 2.0 40.0 72-73 28.582626262740405 34.0 26.0 37.0 2.0 39.5 74-75 28.54778256597552 34.0 27.0 37.0 2.0 39.0 76-77 28.27752094656021 34.0 26.5 36.5 2.0 39.0 78-79 27.974498562316477 34.0 26.0 36.0 2.0 39.0 80-81 27.5486792465623 34.0 26.0 35.5 2.0 37.5 82-83 27.643483301796017 34.0 26.0 35.0 2.0 37.0 84-85 27.462896803760295 34.0 26.0 35.0 2.0 37.0 86-87 27.33197352786462 34.0 26.0 35.0 2.0 36.0 88-89 27.160646231540216 34.0 26.0 35.0 2.0 36.0 90-91 27.020682132664657 34.0 26.0 35.0 2.0 36.0 92-93 26.89475246926171 34.0 26.0 35.0 2.0 35.5 94-95 26.78671206382874 34.0 26.0 35.0 2.0 35.0 96-97 26.673562782614 34.0 25.0 35.0 2.0 35.0 98-99 26.5896852916698 34.0 25.0 35.0 2.0 35.0 100-101 26.495330424253655 33.5 25.0 35.0 2.0 35.0 102-103 25.354563154959628 31.5 23.5 34.0 2.0 35.0 104-105 26.203421342127996 33.0 24.5 35.0 2.0 35.0 106-107 26.365019625835025 33.5 25.0 35.0 2.0 35.0 108-109 26.328858028963857 34.0 25.0 35.0 2.0 35.0 110-111 26.269355898570566 34.0 25.0 35.0 2.0 35.0 112-113 26.18896879472456 34.0 24.5 35.0 2.0 35.0 114-115 26.098654640110155 33.0 24.0 35.0 2.0 35.0 116-117 25.9981696302704 33.0 24.0 35.0 2.0 35.0 118-119 25.879247296821617 33.0 23.5 35.0 2.0 35.0 120-121 25.70959414326888 33.0 23.0 35.0 2.0 35.0 122-123 25.517808178156365 33.0 19.5 35.0 2.0 35.0 124-125 24.60041579536472 32.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3959289952314542 1101 2 1.5505130340993176 1101 3 1.243081730019803 1101 4 1.3272566644959802 1101 5 1.3914294009288106 1101 6 1.1140697145366012 1101 7 1.2830053928158236 1101 8 1.4674572827113437 1101 9 1.8743428127279742 1101 10-11 1.7721936342048927 1101 12-13 1.8408982240081357 1101 14-15 2.222078610071385 1101 16-17 1.7693287949605576 1101 18-19 1.78515414661954 1101 20-21 2.0268163199592095 1101 22-23 1.7204529986994217 1101 24-25 1.753905492734006 1101 26-27 2.0321927506418973 1101 28-29 1.9326595645492546 1101 30-31 1.9891110796771443 1101 32-33 1.9933132639889237 1101 34-35 1.8777708622303777 1101 36-37 1.8636086756941737 1101 38-39 1.8834526873291253 1101 40-41 1.9719162489423496 1101 42-43 2.149367031267232 1101 44-45 2.155042060891166 1101 46-47 1.8217412623343456 1101 48-49 1.5821326254449275 1101 50-51 1.6273341175697134 1101 52-53 1.342538893283276 1101 54-55 1.4611247587766591 1101 56-57 1.5039886233104554 1101 58-59 1.3316399252302276 1101 60-61 1.3624692878569071 1101 62-63 1.2969009467616885 1101 64-65 1.3697906699149627 1101 66-67 1.4019700642242725 1101 68-69 1.2336389955452596 1101 70-71 1.169485696585511 1101 72-73 1.106377551925096 1101 74-75 0.9983588732069038 1101 76-77 1.1680432730792134 1101 78-79 1.2100248502062456 1101 80-81 1.3105365321135736 1101 82-83 1.1884717094672155 1101 84-85 1.183821362486018 1101 86-87 1.0685752256796945 1101 88-89 1.1641407691505385 1101 90-91 1.0995461334090564 1101 92-93 1.1423394294419147 1101 94-95 1.0955191962821615 1101 96-97 1.0187445779837425 1101 98-99 1.0984068979362505 1101 100-101 1.1904370288323705 1101 102-103 1.040613429275286 1101 104-105 1.1270371465512525 1101 106-107 1.2004855561603485 1101 108-109 1.141158855544699 1101 110-111 1.1949322376135711 1101 112-113 1.1823486960871321 1101 114-115 1.1447857774614185 1101 116-117 1.150240632248103 1101 118-119 1.1470648011960058 1101 120-121 1.0699428660568095 1101 122-123 0.9854825368112863 1101 124-125 0.7949143616483525 1102 1 0.8835795409597189 1102 2 1.2509626094116761 1102 3 1.0654608578303382 1102 4 0.916269585176039 1102 5 1.1058758956295058 1102 6 0.9006709995187343 1102 7 0.8932891413115307 1102 8 0.8802891570521254 1102 9 1.4662207196777004 1102 10-11 1.5058334750399602 1102 12-13 1.6210613849108526 1102 14-15 1.8378030881562921 1102 16-17 1.5081160567707315 1102 18-19 1.385553540022478 1102 20-21 1.739470510264809 1102 22-23 1.6861207172114376 1102 24-25 1.2988147442104179 1102 26-27 1.6929618087321074 1102 28-29 1.228425509502486 1102 30-31 1.4528468817977966 1102 32-33 1.636172999743998 1102 34-35 1.5471106303481363 1102 36-37 1.42453330351168 1102 38-39 1.6924910648736429 1102 40-41 1.6741210294661997 1102 42-43 1.8206436526540202 1102 44-45 1.4477734233067743 1102 46-47 1.240533109632647 1102 48-49 1.2131262127312397 1102 50-51 1.3372528420431529 1102 52-53 1.176747215581237 1102 54-55 1.2127118197430313 1102 56-57 1.2251600166018761 1102 58-59 1.1747206153011795 1102 60-61 1.108595290914284 1102 62-63 0.9110320475808642 1102 64-65 0.8574896406050101 1102 66-67 0.8395810713705494 1102 68-69 0.8194387348781227 1102 70-71 0.9175513843564573 1102 72-73 0.5966609054705856 1102 74-75 0.7893683543352523 1102 76-77 0.5599703019616911 1102 78-79 0.7221762485623273 1102 80-81 0.7393921319267847 1102 82-83 0.8138299961034932 1102 84-85 0.7801609000225511 1102 86-87 0.8830521547791648 1102 88-89 0.7527009491084407 1102 90-91 0.9189603757783829 1102 92-93 0.9939535125533787 1102 94-95 0.8432092457438642 1102 96-97 0.6969201955000166 1102 98-99 0.8817797553313476 1102 100-101 0.883023029241258 1102 102-103 0.7774319659150493 1102 104-105 0.8442877877645429 1102 106-107 0.8097348109822242 1102 108-109 0.9305520174464093 1102 110-111 0.785933848778118 1102 112-113 0.7986369946416154 1102 114-115 0.8628122344792182 1102 116-117 0.9169974840534891 1102 118-119 0.8348255118972965 1102 120-121 0.8213565554958393 1102 122-123 0.7529752011918909 1102 124-125 0.8273885621064956 1103 1 0.9187492762876808 1103 2 1.1179690841449457 1103 3 0.8803915465743195 1103 4 0.7030264825605741 1103 5 0.811495292836458 1103 6 0.7392417456580453 1103 7 0.6412584618019501 1103 8 0.7579946579116346 1103 9 1.3418555845003297 1103 10-11 1.4259938960574452 1103 12-13 1.2948190032887155 1103 14-15 1.5898186729777848 1103 16-17 1.30226868537747 1103 18-19 1.1348001375021965 1103 20-21 1.4221129064488736 1103 22-23 1.3346162651663747 1103 24-25 1.1475954929779846 1103 26-27 1.3658841137087379 1103 28-29 1.2870384053384853 1103 30-31 1.4492931926560289 1103 32-33 1.4862885185040255 1103 34-35 1.3066542468933378 1103 36-37 0.9917301932955951 1103 38-39 1.049655359010515 1103 40-41 1.2099926677903667 1103 42-43 1.4697762852386518 1103 44-45 1.140867115147831 1103 46-47 0.9828632322370332 1103 48-49 0.7848812543570602 1103 50-51 0.8649220712551013 1103 52-53 0.656757408935313 1103 54-55 0.7304480782493279 1103 56-57 0.7827724453904672 1103 58-59 0.7826638397396586 1103 60-61 0.8796887856364712 1103 62-63 0.6161384473734444 1103 64-65 0.5868145795159236 1103 66-67 0.5310862692707872 1103 68-69 0.5564954838019567 1103 70-71 0.6986781850639545 1103 72-73 0.5496853482102182 1103 74-75 0.6272416370925029 1103 76-77 0.522879323612802 1103 78-79 0.5793991456058976 1103 80-81 0.5977495723649184 1103 82-83 0.5757363752962661 1103 84-85 0.5842704732513226 1103 86-87 0.396471776453005 1103 88-89 0.43242674078043564 1103 90-91 0.5143676837384348 1103 92-93 0.5021204047524428 1103 94-95 0.5461735317118439 1103 96-97 0.5973292171511311 1103 98-99 0.6258337801079463 1103 100-101 0.5241046435559937 1103 102-103 0.3453207695633438 1103 104-105 0.5026128634155675 1103 106-107 0.5930747593406345 1103 108-109 0.614811765063223 1103 110-111 0.5505210889718981 1103 112-113 0.5427007865693305 1103 114-115 0.5842459812294187 1103 116-117 0.5434228221745023 1103 118-119 0.6410383523269125 1103 120-121 0.5538218554716892 1103 122-123 0.5088341599780613 1103 124-125 0.37714065405268116 1104 1 1.1753013014432838 1104 2 1.5688249285057374 1104 3 1.2276277588643545 1104 4 1.4231109837777645 1104 5 1.0592882434073339 1104 6 1.1145852782036663 1104 7 0.8948652908775436 1104 8 1.1886836474372373 1104 9 1.5881928917616364 1104 10-11 1.7280272029732764 1104 12-13 1.6338239113208957 1104 14-15 1.7407184547254815 1104 16-17 1.4678081522946584 1104 18-19 1.5593533984692627 1104 20-21 1.8494863767010727 1104 22-23 1.7055020078176213 1104 24-25 1.6516652079312877 1104 26-27 1.7982247403294878 1104 28-29 1.6805526992630817 1104 30-31 1.8038216779798262 1104 32-33 1.8266103163894414 1104 34-35 1.7560178576075103 1104 36-37 1.7104551280090163 1104 38-39 1.8872902668586562 1104 40-41 1.859008445688616 1104 42-43 1.9928734101945835 1104 44-45 1.8963753375399826 1104 46-47 1.7753902971311923 1104 48-49 1.585084319859277 1104 50-51 1.4900006680236437 1104 52-53 1.3647276103444668 1104 54-55 1.5118709540063655 1104 56-57 1.446058092457612 1104 58-59 1.5071079528181457 1104 60-61 1.5107043638456723 1104 62-63 1.461256984849701 1104 64-65 1.4721919794978646 1104 66-67 1.4928734224831537 1104 68-69 1.2735473344107895 1104 70-71 1.3005476397259166 1104 72-73 1.3928303985375088 1104 74-75 1.2302060485336703 1104 76-77 1.2077907293497887 1104 78-79 1.272998691393017 1104 80-81 1.347957340268863 1104 82-83 1.3521361585655427 1104 84-85 1.4067130007002326 1104 86-87 1.3359969338675342 1104 88-89 1.3149093016808848 1104 90-91 1.3626696529941462 1104 92-93 1.3081081489458484 1104 94-95 1.2830035825658896 1104 96-97 1.2423841816338665 1104 98-99 1.3411346562907376 1104 100-101 1.3787159462169143 1104 102-103 1.2561307723905841 1104 104-105 1.3394758238361248 1104 106-107 1.3075108718151291 1104 108-109 1.2914658547977993 1104 110-111 1.2984093917874375 1104 112-113 1.3179692400906902 1104 114-115 1.3594191549622252 1104 116-117 1.2638051180159984 1104 118-119 1.3369997500285145 1104 120-121 1.3390378811988235 1104 122-123 1.4660173068600812 1104 124-125 1.2461318867181 1105 1 1.0686393936300682 1105 2 1.5830967578326565 1105 3 0.9895489998397515 1105 4 1.29918556976099 1105 5 1.1535768539543163 1105 6 0.9742850947373363 1105 7 0.9474472819567801 1105 8 1.039869847318819 1105 9 1.3329598575264114 1105 10-11 1.3468417197026632 1105 12-13 1.512584814417707 1105 14-15 1.8550546398921952 1105 16-17 1.3349736636870375 1105 18-19 1.5340781120162035 1105 20-21 1.459698709228043 1105 22-23 1.5119581856651578 1105 24-25 1.3139622870110443 1105 26-27 1.6693261853094228 1105 28-29 1.7182933988006184 1105 30-31 1.5037239962511109 1105 32-33 1.7273183002009915 1105 34-35 1.428719362699276 1105 36-37 1.085857352007693 1105 38-39 1.4105551464506263 1105 40-41 1.53071121772075 1105 42-43 1.6212948275397707 1105 44-45 1.4140868995362261 1105 46-47 0.9055747166171706 1105 48-49 1.1550548414059563 1105 50-51 1.2417449895774233 1105 52-53 1.034747181477151 1105 54-55 1.2192146162780553 1105 56-57 1.205603454975794 1105 58-59 1.1641664665391573 1105 60-61 1.2198564600023438 1105 62-63 1.1218760810710755 1105 64-65 0.8110332682566046 1105 66-67 0.8152654977398299 1105 68-69 0.7199016978410846 1105 70-71 1.0617355719971364 1105 72-73 1.0658265490146839 1105 74-75 1.0395533690000107 1105 76-77 1.1188989647404952 1105 78-79 1.1449006534460295 1105 80-81 1.237200731271983 1105 82-83 1.1688861399545267 1105 84-85 0.9511634748810209 1105 86-87 1.0848114992986382 1105 88-89 1.113168771903613 1105 90-91 0.9158517883696113 1105 92-93 0.9842617708577244 1105 94-95 0.8517354803117669 1105 96-97 1.0032936096600373 1105 98-99 1.035569998152436 1105 100-101 1.0519947057981085 1105 102-103 1.0011837563795467 1105 104-105 1.0659741495406827 1105 106-107 1.0886561413826072 1105 108-109 1.0858353797682128 1105 110-111 1.1085810931679951 1105 112-113 1.0764083779033307 1105 114-115 1.0382368303458058 1105 116-117 1.137787547828129 1105 118-119 1.0656669023208707 1105 120-121 1.0118551572283287 1105 122-123 1.0000836010281908 1105 124-125 0.9779853836057093 1106 1 0.23475357410888975 1106 2 -1.9005128185945992 1106 3 -0.06348967419339857 1106 4 0.3279148515289485 1106 5 0.5686781430887535 1106 6 0.6645245054187399 1106 7 0.5702833777210543 1106 8 0.5183228859928555 1106 9 1.0250408888339635 1106 10-11 1.223821461875783 1106 12-13 1.227133617364295 1106 14-15 1.4073566656748824 1106 16-17 0.9172941056759853 1106 18-19 0.953415128590791 1106 20-21 1.2654077331691127 1106 22-23 0.8756782593299803 1106 24-25 0.9881795982449262 1106 26-27 1.0385711208526942 1106 28-29 1.0514425700713446 1106 30-31 1.1615508839122484 1106 32-33 1.1418671031475824 1106 34-35 1.0292718488871202 1106 36-37 0.7738868179377079 1106 38-39 1.047571721036256 1106 40-41 1.0099009046452494 1106 42-43 1.464941236379552 1106 44-45 1.2919874520224148 1106 46-47 1.0217809781272926 1106 48-49 0.8250364251996984 1106 50-51 0.8367726138868186 1106 52-53 0.8290381575360826 1106 54-55 0.77906728662796 1106 56-57 0.7984211345338039 1106 58-59 0.7549583634084058 1106 60-61 0.835141911199397 1106 62-63 0.6936992854909647 1106 64-65 0.7230038023265877 1106 66-67 0.662410985769295 1106 68-69 0.5714670753733131 1106 70-71 0.6237981870984264 1106 72-73 0.5196018712982919 1106 74-75 0.44820898594291947 1106 76-77 0.3816982280922403 1106 78-79 0.5596336184552371 1106 80-81 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-1.87039743717272 2216 86-87 -2.000568449395068 2216 88-89 -2.111993545436828 2216 90-91 -1.8993171368825124 2216 92-93 -1.878017018244016 2216 94-95 -1.9691189893083418 2216 96-97 -2.01920636075695 2216 98-99 -2.159121438529631 2216 100-101 -2.1019974755663355 2216 102-103 -1.9398506278048089 2216 104-105 -1.9021327429284796 2216 106-107 -1.9288280441595838 2216 108-109 -2.065766680704453 2216 110-111 -1.8896537660884682 2216 112-113 -2.2815090649282688 2216 114-115 -2.162294542910278 2216 116-117 -1.976798872259426 2216 118-119 -2.070126367823139 2216 120-121 -2.2049422040911644 2216 122-123 -2.0690545242555736 2216 124-125 -1.9442414265790653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135201.0 3 17705.0 4 10445.0 5 8589.0 6 8474.0 7 9347.0 8 10254.0 9 10868.0 10 11713.0 11 12816.0 12 13784.0 13 14107.0 14 13894.0 15 11966.0 16 9752.0 17 8586.0 18 7941.0 19 7399.0 20 7270.0 21 7898.0 22 9609.0 23 13576.0 24 19768.0 25 26504.0 26 29910.0 27 32742.0 28 37278.0 29 43019.0 30 49774.0 31 57911.0 32 70766.0 33 89958.0 34 120333.0 35 164981.0 36 251388.0 37 318919.0 38 95204.0 39 212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70718346832518 19.06444905267937 13.817854702465974 23.41051277652948 2 13.46050382666786 20.172095283293483 42.878105026373824 23.489295863664836 3 19.52420183741995 25.18412162736434 28.036236178837378 27.25544035637833 4 11.927042614183577 16.820365523414573 37.73893288917593 33.513658973225915 5 13.335324410511754 38.47497845046213 34.27359780656591 13.916099332460204 6 31.446382048887877 37.62523067131511 17.6211650805389 13.307222199258115 7 28.829813796824688 32.57137259942169 20.502052724821635 18.096760878931985 8 24.814253132010617 37.6115825661457 19.598056514599065 17.976107787244615 9 25.36515945989855 17.95141622942254 20.060943405732168 36.62248090494674 10-11 23.900780119815632 28.107679051613278 27.71586530274408 20.275675525827005 12-13 24.806371100294296 25.005878260170807 28.06098903601909 22.126761603515806 14-15 21.75319529956181 26.651418881670626 25.66385029776762 25.931535520999947 16-17 22.00633515194519 29.874802097231797 26.02312477578564 22.095737975037373 18-19 21.95476380821666 28.680990753252633 27.873304704026264 21.490940734504445 20-21 22.66999981323211 27.746832161883617 28.02664442053564 21.556523604348634 22-23 22.40560737400023 27.62297972693375 27.737214914428332 22.23419798463769 24-25 22.175235820354843 27.64881516721807 27.928629850484377 22.247319161942713 26-27 22.12728977377144 27.783301057307668 27.843190334352062 22.246218834568825 28-29 21.982279420149442 27.858089354437915 28.002794501430785 22.156836723981865 30-31 22.174562247755293 27.689134374534973 27.97039800699453 22.1659053707152 32-33 21.98241058478084 27.699437506633178 27.959868397778326 22.358283510807656 34-35 22.32298980414256 27.695384295929664 27.86656471092123 22.115061189006546 36-37 22.259086588512197 27.593355361426696 27.762102249581318 22.38545580047979 38-39 22.09632104827655 27.81083072701773 27.904103433092114 22.18874479161361 40-41 22.390125092166844 27.675442506994774 27.831089486398934 22.10334291443945 42-43 22.046707051206337 27.53235394168459 28.080992273461515 22.33994673364756 44-45 22.308871123364757 27.767129404055137 27.72010301664938 22.20389645593073 46-47 22.302986649209252 27.93028652809562 27.31758903238382 22.449137790311305 48-49 22.28456254998881 27.78213635094345 27.48741632930556 22.445884769762184 50-51 22.22182297213335 27.979314192718206 27.471505804090306 22.32735703105814 52-53 22.39712711497483 27.922846397432743 27.324102294176413 22.355924193416023 54-55 22.3767468194439 27.794734267271686 27.35825753372254 22.470261379561876 56-57 22.322123807669847 28.101642891742983 27.328439139666173 22.247794160920996 58-59 22.41830795669116 27.857174175936862 27.27831799891091 22.446199868461065 60-61 22.425949690829917 27.795884371293806 27.543269100192834 22.234896837683443 62-63 22.28297014236739 27.859844204074165 27.56708681646921 22.29009883708924 64-65 22.282090760993935 27.86283931699703 27.32495594957276 22.530113972436272 66-67 22.233479078510353 28.005502849144264 27.281585169006988 22.47943290333839 68-69 22.259190150635117 27.826101959639622 27.449464672152175 22.46524321757309 70-71 22.206998728033692 27.769906753583896 27.504595568737518 22.5184989496449 72-73 22.395574998515386 27.812186286680596 27.248060809836357 22.544177904967665 74-75 22.26190987901219 27.995143972780006 27.249716081334213 22.493230066873593 76-77 22.3573928961761 27.831946616100705 27.338939789243117 22.47172069848008 78-79 22.391559235975077 27.8231512845717 27.31752575861508 22.467763720838136 80-81 22.257549735364268 27.930958490847868 27.41798187707063 22.393509896717237 82-83 22.363988854937947 28.060457633289666 27.281610279845843 22.293943231926548 84-85 22.35103000020932 27.903294880520203 27.300082992904024 22.445592126366456 86-87 22.221115628027416 27.86899949297866 27.553667303461328 22.3562175755326 88-89 22.457023867455835 27.791751072281386 27.370392585131786 22.380832475130994 90-91 22.309551128854128 28.003893732008038 27.411092553364835 22.275462585773 92-93 22.329810903361135 27.927461799480334 27.393368816947266 22.34935848021126 94-95 22.263186263621996 27.938199314426054 27.36687826181681 22.431736160135134 96-97 22.33377063075792 27.956065047449645 27.258246998448758 22.451917323343675 98-99 22.22705659497713 27.83175741582722 27.24124098411484 22.699945005080806 100-101 22.13904033724302 27.910910277834034 27.368241817546185 22.58180756737676 102-103 22.200529012498684 27.750746152096184 27.460915438359407 22.587809397045724 104-105 22.179287561817567 27.80545338068025 27.526830627290096 22.488428430212085 106-107 22.10741762057194 27.830340113464498 27.47835522018997 22.583887045773594 108-109 22.079644377959877 27.926593265212425 27.196548560688587 22.79721379613911 110-111 22.025970235450533 27.946468949043464 27.412250124935767 22.615310690570244 112-113 22.125793757962324 27.94732176059793 27.395003372278314 22.53188110916143 114-115 22.002183109743683 28.000984096102076 27.282735334705787 22.714097459448453 116-117 21.91784231003954 27.96140243512728 27.396567609651147 22.724187645182035 118-119 21.931512903359888 27.961252455369323 27.3171942724119 22.79004036885889 120-121 21.95505022645659 28.009282755060177 27.371432626656084 22.664234391827147 122-123 22.034987357050966 28.002555456604046 27.316009811188636 22.64644737515636 124-125 22.05340462849163 28.005728348026555 27.22752593395319 22.713341089528626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2130.0 1 1532.0 2 735.5 3 483.5 4 414.5 5 390.5 6 385.5 7 445.0 8 581.0 9 648.0 10 654.0 11 680.0 12 742.0 13 816.5 14 893.5 15 963.0 16 1066.0 17 1240.0 18 1412.5 19 1672.0 20 2030.0 21 2504.5 22 3203.0 23 4154.5 24 5322.5 25 6932.5 26 9034.5 27 11643.5 28 14408.5 29 17464.5 30 21032.5 31 24871.5 32 28910.0 33 32871.5 34 37442.5 35 41932.0 36 46150.5 37 50328.5 38 53335.5 39 55557.5 40 57965.0 41 60640.5 42 62740.5 43 64883.5 44 67764.0 45 69703.5 46 70178.0 47 70197.5 48 70162.0 49 68964.0 50 66628.0 51 63624.0 52 60344.5 53 57632.5 54 54742.5 55 50369.5 56 45007.5 57 40244.5 58 36200.5 59 31462.0 60 25899.0 61 20792.0 62 16396.5 63 12701.0 64 9586.5 65 7149.0 66 5415.5 67 4153.0 68 3137.5 69 2323.0 70 1705.5 71 1307.5 72 1035.0 73 780.5 74 585.0 75 443.5 76 310.0 77 203.5 78 142.5 79 103.5 80 69.5 81 49.5 82 41.5 83 34.5 84 25.0 85 17.0 86 15.5 87 9.0 88 8.0 89 6.0 90 3.5 91 2.5 92 0.5 93 1.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1953825752417845 2 0.231374102260008 3 0.28222555330616356 4 0.26674411154322286 5 0.3007015805196001 6 0.2158361588847938 7 0.20747392026831485 8 0.1889413914426048 9 0.18668132695166456 10-11 0.1306317275763464 12-13 0.1557466942319199 14-15 0.17216041259737347 16-17 0.14571765805337256 18-19 0.17083262470894606 20-21 0.16735777555412545 22-23 0.18815036887077574 24-25 0.17831908833518564 26-27 0.2317978643520593 28-29 0.1593062958051508 30-31 0.14012399843829543 32-33 0.179307866549972 34-35 0.13715766379393637 36-37 0.13679040331415857 38-39 0.16944833520824515 40-41 0.1900431728819382 42-43 0.2588621366310688 44-45 0.15639646277306524 46-47 0.1419603008371844 48-49 0.14636742659451787 50-51 0.15023778703525306 52-53 0.16927883037142463 54-55 0.1557466942319199 56-57 0.1544189063434925 58-59 0.1312814961174917 60-61 0.14402260968516736 62-63 0.13314604932251742 64-65 0.12907793323882497 66-67 0.115800054354551 68-69 0.1193596559277819 70-71 0.12009417688733748 72-73 0.09752178278407174 74-75 0.09882131986636239 76-77 0.12074394542848281 78-79 0.12704387519697874 80-81 0.1373836702430304 82-83 0.13794868636576543 84-85 0.12656361149265397 86-87 0.15125481605617616 88-89 0.1469606935233897 90-91 0.1358863775177825 92-93 0.13438908479253456 94-95 0.15416464908826175 96-97 0.14501138789995371 98-99 0.13701640976325258 100-101 0.11950090995846566 102-103 0.15792200630444989 104-105 0.11630856886501255 106-107 0.12269325105191878 108-109 0.1337110654452525 110-111 0.11031939796402089 112-113 0.09992310130569576 114-115 0.09836930696817434 116-117 0.10294593756232835 118-119 0.08715373693188334 120-121 0.08026054023451559 122-123 0.10619478026805496 124-125 0.10238092143959328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1769861.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.89164933974254 #Duplication Level Percentage of deduplicated Percentage of total 1 85.40432286377272 62.252619542842766 2 9.143495202765694 13.3296889211923 3 2.4451736689808694 5.346982249623757 4 1.0324476957174136 3.0102726159143565 5 0.5494287522222826 2.0024383972079454 6 0.31996096258981327 1.399348937250189 7 0.21317818979164335 1.0877236900021487 8 0.160951325650469 0.9385606072064557 9 0.11444762922195448 0.7508048811310419 >10 0.5809715486526645 7.422476147462592 >50 0.027149293493441123 1.3406432726534305 >100 0.008156249972662106 0.8683248102903605 >500 7.915429208621898E-5 0.04073398953153867 >1k 2.3746287625865694E-4 0.20938193769115274 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.650161227350622E-5 14-15 0.0 0.0 0.0 0.0 5.650161227350622E-5 16-17 0.0 0.0 0.0 2.825080613675311E-5 5.650161227350622E-5 18-19 0.0 0.0 0.0 5.650161227350622E-5 5.650161227350622E-5 20-21 0.0 0.0 0.0 5.650161227350622E-5 5.650161227350622E-5 22-23 0.0 2.825080613675311E-5 0.0 1.1300322454701244E-4 5.650161227350622E-5 24-25 0.0 5.650161227350622E-5 0.0 1.9775564295727177E-4 5.650161227350622E-5 26-27 0.0 5.650161227350622E-5 0.0 3.9551128591454353E-4 5.650161227350622E-5 28-29 0.0 5.650161227350622E-5 0.0 7.345209595555809E-4 5.650161227350622E-5 30-31 0.0 5.650161227350622E-5 0.0 0.00217531207252999 5.650161227350622E-5 32-33 0.0 5.650161227350622E-5 0.0 0.004887389461658289 5.650161227350622E-5 34-35 2.825080613675311E-5 5.650161227350622E-5 0.0 0.007910225718290871 5.650161227350622E-5 36-37 5.650161227350622E-5 5.650161227350622E-5 0.0 0.013532136139504741 5.650161227350622E-5 38-39 5.650161227350622E-5 5.650161227350622E-5 0.0 0.024662953757385468 5.650161227350622E-5 40-41 1.4125403068376556E-4 5.650161227350622E-5 0.0 0.03912736649940306 5.650161227350622E-5 42-43 2.2600644909402488E-4 5.650161227350622E-5 0.0 0.052970261506412084 5.650161227350622E-5 44-45 2.2600644909402488E-4 5.650161227350622E-5 0.0 0.06774543311593396 5.650161227350622E-5 46-47 2.825080613675311E-4 5.650161227350622E-5 0.0 0.08834027078962697 5.650161227350622E-5 48-49 2.825080613675311E-4 5.650161227350622E-5 0.0 0.11091266489289273 5.650161227350622E-5 50-51 2.825080613675311E-4 5.650161227350622E-5 0.0 0.1375531750798509 5.650161227350622E-5 52-53 2.825080613675311E-4 5.650161227350622E-5 0.0 0.1637699231747578 5.650161227350622E-5 54-55 2.825080613675311E-4 5.650161227350622E-5 0.0 0.19077769384149376 5.650161227350622E-5 56-57 3.3900967364103735E-4 5.650161227350622E-5 0.0 0.22306836525580256 5.650161227350622E-5 58-59 3.3900967364103735E-4 5.650161227350622E-5 0.0 0.261235204346556 5.650161227350622E-5 60-61 3.3900967364103735E-4 5.650161227350622E-5 0.0 0.3029051433982669 5.650161227350622E-5 62-63 3.3900967364103735E-4 5.650161227350622E-5 0.0 0.34567686388931107 5.650161227350622E-5 64-65 3.3900967364103735E-4 5.650161227350622E-5 0.0 0.3902566359731075 5.650161227350622E-5 66-67 3.3900967364103735E-4 5.650161227350622E-5 0.0 0.4422381192647332 5.650161227350622E-5 68-69 3.955112859145436E-4 5.650161227350622E-5 0.0 0.5038531274489917 5.650161227350622E-5 70-71 3.955112859145436E-4 5.650161227350622E-5 0.0 0.5732088565147206 5.650161227350622E-5 72-73 3.955112859145436E-4 5.650161227350622E-5 0.0 0.6482429976139369 5.650161227350622E-5 74-75 4.2376209205129665E-4 5.650161227350622E-5 0.0 0.7176552282919393 5.650161227350622E-5 76-77 4.5201289818804977E-4 5.650161227350622E-5 0.0 0.7970117427300788 5.650161227350622E-5 78-79 4.5201289818804977E-4 5.650161227350622E-5 0.0 0.8930927344011761 5.650161227350622E-5 80-81 6.780193472820747E-4 5.650161227350622E-5 0.0 1.0056721968561373 5.650161227350622E-5 82-83 6.780193472820747E-4 5.650161227350622E-5 0.0 1.124014823762996 5.650161227350622E-5 84-85 7.627717656923341E-4 5.650161227350622E-5 0.0 1.2445327627423848 5.650161227350622E-5 86-87 7.910225718290872E-4 5.650161227350622E-5 0.0 1.37474072822668 5.650161227350622E-5 88-89 7.910225718290872E-4 5.650161227350622E-5 0.0 1.52622155073195 5.650161227350622E-5 90-91 7.910225718290872E-4 5.650161227350622E-5 0.0 1.7016873076473238 5.650161227350622E-5 92-93 7.910225718290872E-4 5.650161227350622E-5 0.0 1.8909112071512961 5.650161227350622E-5 94-95 7.910225718290872E-4 5.650161227350622E-5 0.0 2.0725356398044816 5.650161227350622E-5 96-97 7.910225718290872E-4 5.650161227350622E-5 0.0 2.2598949861034283 5.650161227350622E-5 98-99 8.192733779658403E-4 5.650161227350622E-5 0.0 2.470024482148598 5.650161227350622E-5 100-101 0.001017029020923112 5.650161227350622E-5 0.0 2.7203266245202307 5.650161227350622E-5 102-103 0.0011865338577436307 5.650161227350622E-5 0.0 2.9940204343730947 5.650161227350622E-5 104-105 0.0015255435313846681 5.650161227350622E-5 0.0 3.2708783345132755 5.650161227350622E-5 106-107 0.0015537943375214212 5.650161227350622E-5 0.0 3.5423968322936092 5.650161227350622E-5 108-109 0.0015820451436581744 5.650161227350622E-5 0.0 3.835950958860611 5.650161227350622E-5 110-111 0.0015820451436581744 5.650161227350622E-5 0.0 4.173463339776401 5.650161227350622E-5 112-113 0.0015820451436581744 5.650161227350622E-5 0.0 4.553125923448226 5.650161227350622E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3925 0.0 42.043133 1 ATCAACG 4285 0.0 36.285435 3 CAACGCA 4500 0.0 34.948944 5 TCAACGC 4535 0.0 34.67922 4 AACGCAG 4530 0.0 33.77303 6 GGTATCA 2795 0.0 33.463608 1 GTACATG 6275 0.0 33.418266 1 TATCAAC 5110 0.0 31.82617 2 TACATGG 6490 0.0 30.658045 2 ACATGGG 6440 0.0 28.213478 3 ACGCAGA 5385 0.0 27.958252 7 TACCTGG 1885 0.0 26.862734 2 CGCAGAG 5270 0.0 26.642704 8 GTACCTG 2095 0.0 25.876904 1 CGCATAG 190 1.1697539E-6 25.050318 8 GTGTAGC 1045 0.0 23.943518 1 CATGGGA 3535 0.0 21.90769 4 GCAGAGT 5965 0.0 21.642822 9 CATGGGG 3305 0.0 21.629797 4 GTATAGG 640 0.0 21.409317 1 >>END_MODULE