FastQCFastQC Report
Thu 26 May 2016
SRR1364607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769861
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.24273092632698273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.21097702022927226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.13379581786366274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.050.01431
GTATCAA60450.045.8225171
ATCAACG72350.037.910253
TCAACGC74200.037.1275184
CAACGCA74300.036.7113725
TATCAAC76700.036.0918922
AACGCAG76400.035.7731636
GTACATG65300.031.8371751
ACGCAGA90100.030.267657
TACATGG65400.030.2212452
CGCAGAG90550.029.9199568
ACATGGG65500.028.0679993
GCAGAGT102000.025.6279959
GAGTACT61150.024.3475512-13
CGTCTAG1250.001622845723.8277071
TACCTGG16050.023.3677862
GTGGTAT20050.022.8769781
CAGAGTA99850.022.55420110-11
GTACTTT65650.022.40649614-15
AGTACTT65750.022.05539912-13
Produced by FastQC (version 0.11.5)