##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364607_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1769861 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.65061719536167 31.0 30.0 33.0 26.0 34.0 2 29.837370844377045 31.0 30.0 34.0 26.0 34.0 3 29.857435696927613 31.0 30.0 34.0 26.0 34.0 4 33.51390758935306 35.0 33.0 37.0 28.0 37.0 5 32.920901132913826 35.0 33.0 37.0 26.0 37.0 6 32.91239142508932 35.0 33.0 37.0 26.0 37.0 7 32.712275144771255 35.0 33.0 37.0 25.0 37.0 8 32.64831757974214 35.0 33.0 37.0 25.0 37.0 9 33.978763869027006 38.0 34.0 39.0 25.0 39.0 10-11 33.76520670267326 38.0 33.0 39.0 23.0 39.0 12-13 33.629061830279326 37.5 33.0 39.0 22.0 39.0 14-15 34.6254736953919 39.0 33.0 41.0 22.0 41.0 16-17 34.54196205238716 39.0 33.0 41.0 20.5 41.0 18-19 34.578438080730635 39.0 33.5 41.0 21.5 41.0 20-21 34.60127264231485 39.0 34.0 41.0 22.0 41.0 22-23 34.58932057376257 39.0 34.0 41.0 22.0 41.0 24-25 34.58198468693304 39.0 34.0 41.0 22.0 41.0 26-27 34.485026225223336 39.0 33.5 41.0 21.5 41.0 28-29 34.260358581832136 39.0 33.0 41.0 19.0 41.0 30-31 34.21521803124652 38.5 33.0 41.0 19.0 41.0 32-33 34.05130628902496 38.0 33.0 41.0 18.5 41.0 34-35 33.927549677630054 38.0 33.0 41.0 18.0 41.0 36-37 33.780247149352405 38.0 33.0 41.0 16.0 41.0 38-39 33.66043802309899 38.0 33.0 41.0 15.5 41.0 40-41 33.4983312248815 38.0 33.0 40.0 15.0 41.0 42-43 33.342144100581905 38.0 32.0 40.0 11.0 41.0 44-45 33.14754915781522 38.0 32.0 40.0 9.0 41.0 46-47 32.91812294863834 38.0 32.0 40.0 7.5 41.0 48-49 32.69018442691262 38.0 31.5 40.0 2.0 41.0 50-51 32.5013792043556 38.0 31.0 40.0 2.0 41.0 52-53 32.34382728361154 38.0 31.0 40.0 2.0 41.0 54-55 32.131022153717154 38.0 31.0 40.0 2.0 41.0 56-57 31.91819329314562 37.0 30.0 40.0 2.0 41.0 58-59 31.737973773081613 37.0 30.0 40.0 2.0 41.0 60-61 31.531610109494473 37.0 30.0 40.0 2.0 41.0 62-63 31.272070518532246 36.0 30.0 40.0 2.0 41.0 64-65 31.008283136359296 36.0 29.0 39.0 2.0 41.0 66-67 30.73517355317734 35.5 29.0 39.0 2.0 41.0 68-69 30.421311617126996 35.0 29.0 39.0 2.0 40.5 70-71 30.165359878544134 35.0 29.0 38.5 2.0 40.0 72-73 30.010791525436176 35.0 29.0 37.5 2.0 40.0 74-75 29.720233679368043 35.0 29.0 37.0 2.0 39.5 76-77 29.41668696016241 35.0 29.0 37.0 2.0 39.0 78-79 29.10069943345833 34.0 29.0 36.0 2.0 39.0 80-81 28.81527786645392 34.0 28.5 36.0 2.0 37.5 82-83 28.55156252383662 34.0 28.0 35.5 2.0 37.0 84-85 28.519314793647638 34.0 29.0 35.0 2.0 37.0 86-87 28.36116141324093 34.0 29.0 35.0 2.0 36.0 88-89 28.212045465717367 34.0 29.0 35.0 2.0 36.0 90-91 28.054596942923766 34.0 28.5 35.0 2.0 36.0 92-93 27.91634399537591 34.0 28.0 35.0 2.0 35.5 94-95 27.82672876570533 34.0 28.0 35.0 2.0 35.0 96-97 27.738946448336904 34.0 27.5 35.0 2.0 35.0 98-99 27.651956848588675 34.0 27.0 35.0 2.0 35.0 100-101 27.55721353258815 34.0 27.0 35.0 2.0 35.0 102-103 26.391297113163127 32.5 24.5 34.5 2.0 35.0 104-105 27.314097265265467 34.0 26.5 35.0 2.0 35.0 106-107 27.48455613180922 34.0 27.0 35.0 2.0 35.0 108-109 27.49778033416184 34.0 27.0 35.0 2.0 35.0 110-111 27.442098560282417 34.0 27.0 35.0 2.0 35.0 112-113 27.394839764252673 34.0 27.0 35.0 2.0 35.0 114-115 27.319578486672118 34.0 27.0 35.0 2.0 35.0 116-117 27.20366571160108 34.0 27.0 35.0 2.0 35.0 118-119 27.10476444195335 34.0 27.0 35.0 2.0 35.0 120-121 26.974477939227995 34.0 27.0 35.0 2.0 35.0 122-123 26.79824545543407 34.0 26.0 35.0 2.0 35.0 124-125 25.999622851738074 33.0 22.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6744229076459831 1101 2 0.8256123854998982 1101 3 0.6902081154821502 1101 4 0.6726283643565765 1101 5 0.920810343591711 1101 6 0.9055119900576187 1101 7 0.9720166843430746 1101 8 0.8882639545620066 1101 9 1.0474577589916834 1101 10-11 1.0700217025491128 1101 12-13 1.1407894245419286 1101 14-15 1.1701421081286298 1101 16-17 1.2396033089374328 1101 18-19 1.2062611779534507 1101 20-21 1.2683444714533039 1101 22-23 1.1112393284184208 1101 24-25 1.241232680071903 1101 26-27 1.193045499833083 1101 28-29 1.2892269180221163 1101 30-31 1.3290826876435062 1101 32-33 1.2948845689541315 1101 34-35 1.2019231041224074 1101 36-37 1.313495518089475 1101 38-39 1.3632583329855592 1101 40-41 1.432938484840271 1101 42-43 1.4027811749342618 1101 44-45 1.308752792968228 1101 46-47 1.5097086410453926 1101 48-49 1.4137949161155419 1101 50-51 1.3935124738602838 1101 52-53 1.3060202784739587 1101 54-55 1.3158453866846145 1101 56-57 1.2454708969069337 1101 58-59 1.3569157627752695 1101 60-61 1.3775866481436978 1101 62-63 1.262506609362493 1101 64-65 1.1785255080723545 1101 66-67 1.146549053449231 1101 68-69 1.272987960405846 1101 70-71 1.3096329076820936 1101 72-73 1.2088357191836323 1101 74-75 1.1359660618484675 1101 76-77 1.182905850747197 1101 78-79 1.1172226895904949 1101 80-81 1.0882364432510059 1101 82-83 1.0925441863818328 1101 84-85 1.2324891454739308 1101 86-87 1.1625668349257623 1101 88-89 1.0524051601965603 1101 90-91 1.0549796476583815 1101 92-93 0.9797510454880864 1101 94-95 1.0163344532465324 1101 96-97 1.0933397801613545 1101 98-99 1.1181017984281745 1101 100-101 1.105209425830875 1101 102-103 1.055138645054548 1101 104-105 1.0133834089388642 1101 106-107 1.1015895254894978 1101 108-109 1.1348776007615662 1101 110-111 1.1246501031507385 1101 112-113 1.1575746647142608 1101 114-115 1.1506435989658286 1101 116-117 1.1722651987525978 1101 118-119 1.2587878589018686 1101 120-121 1.2657726331328618 1101 122-123 1.22371861014987 1101 124-125 1.2869855727279642 1102 1 0.5138119022421357 1102 2 -0.38319953456217704 1102 3 0.09539683198688564 1102 4 0.3625303005419127 1102 5 0.7011815793676988 1102 6 0.6719176701386544 1102 7 0.6654692519841063 1102 8 0.5810170828000167 1102 9 0.8553882431077113 1102 10-11 0.8600161471097181 1102 12-13 1.119332810403641 1102 14-15 1.157682808328886 1102 16-17 1.324657071535185 1102 18-19 1.262116648827032 1102 20-21 1.2546622054148386 1102 22-23 1.260009595753246 1102 24-25 1.1981126837426217 1102 26-27 1.2184946685790479 1102 28-29 1.2701349230821322 1102 30-31 1.1981268529510771 1102 32-33 1.1413902179037976 1102 34-35 1.0833370520215055 1102 36-37 1.0254112821787444 1102 38-39 0.7990522787472756 1102 40-41 0.8942733671915732 1102 42-43 0.8112035463082705 1102 44-45 0.5314528759077675 1102 46-47 0.6347217092509752 1102 48-49 0.6755181129354924 1102 50-51 0.6051258541926927 1102 52-53 0.6220176932924915 1102 54-55 0.6194778831881464 1102 56-57 0.507749129458265 1102 58-59 0.5804050201294721 1102 60-61 0.4818967746671632 1102 62-63 0.8852224322296429 1102 64-65 0.8043246288434815 1102 66-67 0.5812536190736886 1102 68-69 0.43877840388847034 1102 70-71 0.30600735184774663 1102 72-73 0.38007749202220964 1102 74-75 0.4953300264748677 1102 76-77 0.47187473157445226 1102 78-79 0.5213098289661033 1102 80-81 0.45110914847410655 1102 82-83 0.301429506198577 1102 84-85 0.46407473699319013 1102 86-87 0.5177097691502901 1102 88-89 0.622670433153619 1102 90-91 0.48982549510537865 1102 92-93 0.6430700734923818 1102 94-95 0.46911394441126575 1102 96-97 0.44201809088960076 1102 98-99 0.4483365876176926 1102 100-101 0.16569409072723218 1102 102-103 0.24400790259461402 1102 104-105 0.2737920168787582 1102 106-107 0.555614634449288 1102 108-109 0.6690923410004892 1102 110-111 0.4908851124669198 1102 112-113 0.5704569968488045 1102 114-115 0.5600803534973053 1102 116-117 0.5208042604902836 1102 118-119 0.49590021236372195 1102 120-121 0.533345231370923 1102 122-123 0.5425819550529241 1102 124-125 0.3813121709985481 1103 1 0.12932133908595844 1103 2 -1.0186059901163134 1103 3 -0.02197235922196583 1103 4 0.23759423098559296 1103 5 0.4326342033572388 1103 6 0.5585241533959859 1103 7 0.4660502198910237 1103 8 0.4074921002032994 1103 9 0.5048522928882164 1103 10-11 0.6695365000768021 1103 12-13 0.7259992018142256 1103 14-15 0.7275483501749136 1103 16-17 0.8382163190738581 1103 18-19 0.823379994307146 1103 20-21 0.8508104849755611 1103 22-23 0.7196346348218583 1103 24-25 0.6574000901555479 1103 26-27 0.734506136990305 1103 28-29 0.8525073019339331 1103 30-31 0.7563974545365539 1103 32-33 0.6212374774187737 1103 34-35 0.8323485719322861 1103 36-37 0.7661040750867301 1103 38-39 0.651602703884727 1103 40-41 0.8423185606877155 1103 42-43 0.8268851513886091 1103 44-45 0.7226941387471371 1103 46-47 0.7765593898544765 1103 48-49 0.7735177115943799 1103 50-51 0.9047254453930265 1103 52-53 0.8274464090719462 1103 54-55 0.7464952438552928 1103 56-57 0.6299461790682876 1103 58-59 0.6966030411270729 1103 60-61 0.7956096772513774 1103 62-63 0.8615091212009283 1103 64-65 0.8138922193050604 1103 66-67 0.8014291791754111 1103 68-69 0.6487599439728591 1103 70-71 0.6453275048546097 1103 72-73 0.616262078747404 1103 74-75 0.5102162746812624 1103 76-77 0.5854780200854428 1103 78-79 0.7650182297815711 1103 80-81 0.7359352576673039 1103 82-83 0.5834357944680484 1103 84-85 0.612389370671746 1103 86-87 0.5948882434147329 1103 88-89 0.5410681053751354 1103 90-91 0.6116968990179217 1103 92-93 0.6031681534480526 1103 94-95 0.6200506142034179 1103 96-97 0.6158307604828579 1103 98-99 0.6840097307934734 1103 100-101 0.610690601190516 1103 102-103 0.6575402016754346 1103 104-105 0.7166908096588784 1103 106-107 0.6154377691010104 1103 108-109 0.623822927455759 1103 110-111 0.6782723462676543 1103 112-113 0.6840687206266658 1103 114-115 0.6864672889559671 1103 116-117 0.647100183858992 1103 118-119 0.6533602291987393 1103 120-121 0.6283904887975069 1103 122-123 0.6569881477819024 1103 124-125 0.5941304025404044 1104 1 0.477665840079986 1104 2 -0.4379219783256758 1104 3 0.37901397135267345 1104 4 0.45410671828946647 1104 5 0.8532867170264495 1104 6 0.7230716961266097 1104 7 1.026035983713946 1104 8 0.959410745320362 1104 9 0.9675505092650667 1104 10-11 1.1784414288169387 1104 12-13 1.1123906250971913 1104 14-15 1.2249330467772666 1104 16-17 1.3649968954448468 1104 18-19 1.2787831843179447 1104 20-21 1.2449964470673933 1104 22-23 1.2087147015800213 1104 24-25 1.1995589693116528 1104 26-27 1.0806229747910692 1104 28-29 1.1489527934175143 1104 30-31 1.2001449919344083 1104 32-33 1.1881827958047069 1104 34-35 1.2020756688810295 1104 36-37 1.1889734559883678 1104 38-39 1.2175245796749508 1104 40-41 1.2394555878046987 1104 42-43 1.254057266102862 1104 44-45 1.2397044430207913 1104 46-47 1.2768926155180296 1104 48-49 1.3475159080168169 1104 50-51 1.2578292261674875 1104 52-53 0.9678909186302889 1104 54-55 1.3005604737752066 1104 56-57 1.2625337651044966 1104 58-59 1.200063834906981 1104 60-61 1.2569055780178005 1104 62-63 1.2796278445995775 1104 64-65 1.3507129667941982 1104 66-67 1.352878670960994 1104 68-69 1.2175393439463953 1104 70-71 1.1954021418965795 1104 72-73 1.1907518898963438 1104 74-75 1.1441013323324185 1104 76-77 1.2189848568268928 1104 78-79 1.1631466014362175 1104 80-81 1.154352862531951 1104 82-83 1.2425067418998346 1104 84-85 1.202674252167597 1104 86-87 1.1131948600055317 1104 88-89 1.012657846111221 1104 90-91 0.9819431440160926 1104 92-93 1.0895166972033579 1104 94-95 0.963247035914808 1104 96-97 1.0572335456088915 1104 98-99 1.054355820315319 1104 100-101 1.1209471987181132 1104 102-103 1.0403642482363118 1104 104-105 1.072621972873666 1104 106-107 1.1098329989383195 1104 108-109 0.9682119767866304 1104 110-111 0.9258056141296436 1104 112-113 1.0426951787878274 1104 114-115 1.0720937205981897 1104 116-117 1.1413543416443517 1104 118-119 0.9726639578337881 1104 120-121 0.939542542442851 1104 122-123 1.0377748009021737 1104 124-125 1.053833065838731 1105 1 0.6632010128075798 1105 2 0.3687938492452041 1105 3 0.6233348989673111 1105 4 0.5620852422239295 1105 5 0.7307783838148652 1105 6 0.6167948101700347 1105 7 0.7699773858085379 1105 8 0.6094837631083152 1105 9 0.734818363836176 1105 10-11 0.9126645425124948 1105 12-13 0.9349313797372503 1105 14-15 0.9642182168958371 1105 16-17 1.0759135939415643 1105 18-19 1.1148426310247004 1105 20-21 1.144975366181498 1105 22-23 1.063835861014823 1105 24-25 1.0874572884762657 1105 26-27 1.0895015064482223 1105 28-29 1.1895851225910263 1105 30-31 1.114507684749725 1105 32-33 1.1878310302631192 1105 34-35 1.1013182180401984 1105 36-37 1.093361967670866 1105 38-39 1.102150421779811 1105 40-41 0.9477692491232261 1105 42-43 1.1871269144277719 1105 44-45 1.1857589430246165 1105 46-47 1.2113408007181263 1105 48-49 1.323870459296586 1105 50-51 1.22544272377867 1105 52-53 1.1643866909734157 1105 54-55 1.1483180032345288 1105 56-57 1.2345466279235815 1105 58-59 1.098825489401701 1105 60-61 1.100779354300201 1105 62-63 1.1321731006697213 1105 64-65 1.110634097842869 1105 66-67 1.0431619131873262 1105 68-69 0.9954485235938115 1105 70-71 1.0651169794311315 1105 72-73 1.0587929190053593 1105 74-75 1.1076060052621237 1105 76-77 1.0319642121692283 1105 78-79 1.0474431503630086 1105 80-81 0.8756180496404653 1105 82-83 0.9470874166412457 1105 84-85 0.7685982176561694 1105 86-87 0.8873138633457742 1105 88-89 0.8516039131345217 1105 90-91 0.8556058818457082 1105 92-93 0.7466062408752343 1105 94-95 0.7704154415806279 1105 96-97 0.9171425708022909 1105 98-99 0.9046456303160078 1105 100-101 0.9012993956177588 1105 102-103 0.5634352949962214 1105 104-105 0.5151638963549203 1105 106-107 0.912912269330949 1105 108-109 0.9136071251216258 1105 110-111 0.8562080781582075 1105 112-113 0.6180162100675481 1105 114-115 0.8060560713883085 1105 116-117 0.639728191327201 1105 118-119 0.5237771051404749 1105 120-121 0.5061704564578875 1105 122-123 0.5713137946273044 1105 124-125 0.5356033175779054 1106 1 0.37774981575417144 1106 2 0.33748629860063417 1106 3 0.4115485269599439 1106 4 0.23096185364197197 1106 5 0.2232277392476476 1106 6 0.44441911956229774 1106 7 0.4775280303757512 1106 8 0.557181737325628 1106 9 0.4850946069300974 1106 10-11 0.7340273417758425 1106 12-13 0.5130160942860513 1106 14-15 0.6172568909289851 1106 16-17 0.7637588978089696 1106 18-19 0.8354687820375943 1106 20-21 0.7632074103803888 1106 22-23 0.7402631170000973 1106 24-25 0.694823770980868 1106 26-27 0.6764260000025573 1106 28-29 0.739861365684952 1106 30-31 0.68762002360792 1106 32-33 0.682858654572513 1106 34-35 0.6040806489794264 1106 36-37 0.6828647301018052 1106 38-39 0.6579515267521927 1106 40-41 0.7562407040035168 1106 42-43 0.657660984409361 1106 44-45 0.7334569172419236 1106 46-47 0.5168656625965795 1106 48-49 0.5746991885783572 1106 50-51 0.6267318582169779 1106 52-53 0.712452989315949 1106 54-55 0.595831815389225 1106 56-57 0.4063698323434757 1106 58-59 0.3251606643556606 1106 60-61 0.4644994279650305 1106 62-63 0.4623756789385993 1106 64-65 0.4719600646936968 1106 66-67 0.24776596475270551 1106 68-69 0.5656142694501618 1106 70-71 0.668247734495587 1106 72-73 0.7040967864086767 1106 74-75 0.6961879573799905 1106 76-77 0.7115222232189531 1106 78-79 0.626264513162269 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-1.7667910168020882 2216 116-117 -1.9633008604405866 2216 118-119 -2.0232212774470213 2216 120-121 -1.8989444782321563 2216 122-123 -1.9537254573645697 2216 124-125 -2.0477656710752967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66544.0 3 21224.0 4 9958.0 5 7464.0 6 7900.0 7 9009.0 8 10590.0 9 11320.0 10 12187.0 11 13444.0 12 14828.0 13 16028.0 14 17709.0 15 15193.0 16 10590.0 17 8766.0 18 8170.0 19 7527.0 20 7431.0 21 7811.0 22 9396.0 23 12360.0 24 16638.0 25 22252.0 26 26689.0 27 29635.0 28 34453.0 29 40446.0 30 47688.0 31 56073.0 32 68131.0 33 85177.0 34 108382.0 35 148559.0 36 234813.0 37 382957.0 38 161764.0 39 755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94096615232922 19.33367557164729 13.76685655616289 24.9585017198606 2 15.379100278247554 20.53971318054314 39.55966660051149 24.521519940697818 3 19.522380571129595 24.51678408643391 29.24947213368733 26.711363208749162 4 12.887929037281646 17.07481078906357 36.57978276764999 33.4574774060048 5 13.089159105102677 38.2533846888573 34.43885235663642 14.2186038494036 6 31.931901842917256 37.433984779561285 17.014312455752076 13.619800921769388 7 28.29935701128903 32.37188252178139 20.630049834450183 18.698710632479404 8 25.574381264969396 37.21128382398391 18.959398506436383 18.254936404610305 9 25.76829827109935 16.643642155591454 19.64270478960682 37.94535478370237 10-11 24.75334847361842 27.338362757386257 27.123894230578966 20.784394538416354 12-13 25.490874159462056 24.328530259365994 28.02780132225801 22.15279425891394 14-15 22.26248243030393 25.88999781040974 25.872283318853782 25.975236440432546 16-17 21.89057552982771 29.089228026110003 26.571307996125277 22.44888844793701 18-19 21.793917423342034 27.668031088361015 28.975621497424793 21.56242999087215 20-21 22.38721040815646 26.819062558449534 29.262457979904944 21.531269053489062 22-23 22.1326623880247 26.6070971961851 29.144106789930735 22.11613362585946 24-25 21.89914914222077 26.79394031508689 29.31597453133325 21.990936011359082 26-27 21.88411971335602 26.99994519343609 29.107992096554476 22.00794299665341 28-29 21.731212401554718 26.920621083988454 29.279689893294826 22.068476621162 30-31 21.928106218510944 26.85063968300335 29.263145523857524 21.958108574628177 32-33 21.76482603547688 26.90526418090627 29.244284878436865 22.08562490517999 34-35 22.020458103771993 26.929826692604674 29.142881842133367 21.90683336148997 36-37 21.97022474671711 26.845613125113353 29.032793007804575 22.151369120364954 38-39 21.886266774622413 27.074301315188027 29.099036591009124 21.940395319180432 40-41 22.115415310980723 26.8777963008949 28.991522213191377 22.015266174932997 42-43 21.94872991585509 26.80788381086267 28.948346822586004 22.295039450696237 44-45 22.07407813381955 27.082946061865876 28.716266418662258 22.12670938565232 46-47 22.088793514059596 27.338788005146174 28.25696849297034 22.31544998782389 48-49 22.25396048722553 27.254333398498638 28.16584720356096 22.32585891071487 50-51 22.248643108414594 27.456276054409006 28.021266160937568 22.273814676238832 52-53 22.31935793809738 27.48456728651778 27.87454152460038 22.321533250784455 54-55 22.322543973792293 27.384890677855495 27.921797248484488 22.37076809986773 56-57 22.174926405360967 27.678223332184448 27.88903454004057 22.25781572241402 58-59 22.256634843075247 27.403055946201427 27.94233558454591 22.39797362617742 60-61 22.254487781808855 27.39031483263375 28.11090814476391 22.244289240793485 62-63 22.139280994383174 27.42667362013175 28.181958922197847 22.25208646328723 64-65 22.187464156627698 27.289865316498034 28.109594412643307 22.413076114230957 66-67 22.10224418753789 27.50546511844715 27.955528711011766 22.436761983003183 68-69 22.098552224802788 27.36438354925987 28.063593964133187 22.473470261804156 70-71 22.009807549858433 27.23807123836279 28.076922424981397 22.67519878679738 72-73 22.154739414752854 27.309094330059864 27.95514380307526 22.58102245211202 74-75 22.09663516913994 27.480680961328584 27.895242542821407 22.527441326710065 76-77 22.169885812582795 27.36766065665887 27.948386688502747 22.514066842255588 78-79 22.16134440961721 27.327495730564355 27.954781414782914 22.55637844503552 80-81 22.084358973562143 27.43702147579753 27.943030670468538 22.53558888017179 82-83 22.18886425101216 27.519516390717115 27.84445762189463 22.4471617363761 84-85 22.149815324271593 27.39616545724829 27.918043188751195 22.53597602972892 86-87 22.02934032299894 27.433326308259847 28.04038306049492 22.496950308246287 88-89 22.173210212553414 27.280136688700413 27.989175421120642 22.55747767762553 90-91 22.095237987615977 27.446223177978386 27.971151406805394 22.487387427600247 92-93 22.056333157602992 27.440123135549115 27.97293639748282 22.530607309365074 94-95 22.07316983590551 27.350513314419423 27.914399191308714 22.661917658366356 96-97 22.062467668702627 27.33930268083843 27.927771140042157 22.67045851041679 98-99 22.01570702075407 27.30015134089388 27.777283384340613 22.90685825401144 100-101 21.880797613138473 27.324688371451796 27.936404977694508 22.858109037715227 102-103 21.964041243916895 27.182784410753165 27.97951364542187 22.873660699908072 104-105 21.933604730331016 27.182989566941924 28.016871438620676 22.86653426410638 106-107 21.82856403471673 27.259233267761307 27.940747295118484 22.97145540240348 108-109 21.88375543036211 27.187252531296714 27.783366938929916 23.14562509941126 110-111 21.787464234216724 27.31762840650723 27.89086972440571 23.00403763487034 112-113 21.87938514772801 27.230398963512464 27.852636152701105 23.037579736058426 114-115 21.66149439006923 27.280437211575542 27.82090597945309 23.23716241890214 116-117 21.63371093219183 27.22226255895009 27.881706848168758 23.26231966068933 118-119 21.599413051136818 27.19726550084881 27.82726933454927 23.376052113465104 120-121 21.53040887790396 27.22039422567818 27.865674180382292 23.38352271603557 122-123 21.556322223553156 27.253437010552297 27.825999010086722 23.36424175580783 124-125 21.495971075138982 27.11830026324607 27.827296114252974 23.558432547361974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 66.0 1 62.0 2 119.0 3 229.5 4 204.0 5 162.5 6 384.0 7 853.5 8 1343.0 9 1578.5 10 1395.0 11 1117.5 12 1114.5 13 1252.5 14 1439.5 15 1620.0 16 1814.5 17 1967.5 18 2107.5 19 2347.5 20 2621.0 21 3080.5 22 3680.0 23 4504.0 24 5692.5 25 7138.0 26 9027.0 27 11382.5 28 13897.0 29 17071.5 30 20612.5 31 24074.0 32 27950.0 33 32040.0 34 36351.5 35 40438.5 36 44775.0 37 49036.5 38 52069.0 39 54495.0 40 56790.5 41 59266.0 42 61492.0 43 64137.0 44 67474.5 45 69521.5 46 70037.5 47 70045.5 48 70370.5 49 69699.0 50 67734.5 51 64767.5 52 61350.0 53 58930.5 54 56134.0 55 51810.5 56 46182.0 57 40972.0 58 36819.5 59 32222.5 60 26797.0 61 21154.0 62 16341.0 63 12676.5 64 9558.5 65 7018.0 66 5182.0 67 3842.0 68 2833.5 69 2039.5 70 1491.5 71 1153.0 72 871.0 73 688.5 74 509.5 75 342.0 76 201.0 77 110.5 78 75.0 79 48.5 80 33.0 81 17.0 82 11.5 83 8.5 84 4.5 85 3.5 86 4.0 87 4.0 88 2.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06288629446041243 2 0.07300008305737003 3 0.0 4 0.008079730555111391 5 0.02802479968765909 6 2.2600644909402488E-4 7 5.650161227350622E-5 8 0.0 9 4.5201289818804977E-4 10-11 0.06045672513265166 12-13 0.009096759576034502 14-15 0.006977949115778019 16-17 0.054269798588702726 18-19 1.4125403068376556E-4 20-21 0.00971827731104307 22-23 0.012345602281761108 24-25 0.0 26-27 0.0 28-29 0.05206623571003599 30-31 0.0 32-33 0.0034183475425471265 34-35 0.0 36-37 1.1300322454701244E-4 38-39 0.0 40-41 2.825080613675311E-5 42-43 0.0032205918995898545 44-45 0.0 46-47 1.1300322454701244E-4 48-49 1.6950483682051868E-4 50-51 3.9551128591454353E-4 52-53 2.825080613675311E-5 54-55 0.0 56-57 0.001751549980478693 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.5201289818804977E-4 66-67 0.0 68-69 4.237620920512967E-4 70-71 0.0 72-73 0.0015537943375214212 74-75 0.0022318136848034956 76-77 4.237620920512967E-4 78-79 7.62771765692334E-4 80-81 0.001779800786615446 82-83 1.4125403068376556E-4 84-85 1.1300322454701244E-4 86-87 5.650161227350622E-4 88-89 0.0 90-91 0.0 92-93 0.0058761676764446475 94-95 0.004972141880068548 96-97 0.005508907196666857 98-99 8.757749902393465E-4 100-101 3.1075886750428424E-4 102-103 0.0 104-105 3.3900967364103735E-4 106-107 0.0029098330320855704 108-109 0.0037573572161881644 110-111 7.345209595555809E-4 112-113 0.0021188104602564836 114-115 4.8026370432480294E-4 116-117 0.0021188104602564836 118-119 0.00172329917434194 120-121 0.00926626441285502 122-123 5.08514510461556E-4 124-125 0.0019210548172992118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1769861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.54844312551172 #Duplication Level Percentage of deduplicated Percentage of total 1 83.47278775609301 57.21929644026454 2 10.20128657295606 13.985646249066496 3 2.797363603807803 5.752647596909872 4 1.1561752430283363 3.1701605155941044 5 0.6398867087421456 2.1931618830490924 6 0.3725595853721916 1.5323027729249945 7 0.2527776681368278 1.2129260925373773 8 0.1815885029824469 0.9958087335151262 9 0.14148908053896087 0.8728970571185315 >10 0.7275560162336223 8.85032892012093 >50 0.04163912572529181 1.929535696707516 >100 0.014476663340163724 1.5716696656058748 >500 8.269460861679213E-5 0.0532847147502135 >1k 3.307784344671685E-4 0.6603336618353586 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4296 0.24273092632698273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.21097702022927226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2368 0.13379581786366274 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.650161227350622E-5 0.0 10-11 0.0 0.0 0.0 5.650161227350622E-5 0.0 12-13 0.0 0.0 0.0 1.6950483682051868E-4 0.0 14-15 0.0 0.0 0.0 1.6950483682051868E-4 0.0 16-17 0.0 0.0 0.0 1.6950483682051868E-4 0.0 18-19 0.0 0.0 0.0 1.6950483682051868E-4 5.650161227350622E-5 20-21 0.0 0.0 0.0 1.6950483682051868E-4 5.650161227350622E-5 22-23 0.0 0.0 0.0 1.9775564295727177E-4 5.650161227350622E-5 24-25 1.1300322454701244E-4 0.0 0.0 2.2600644909402488E-4 5.650161227350622E-5 26-27 1.1300322454701244E-4 0.0 0.0 4.5201289818804977E-4 5.650161227350622E-5 28-29 1.1300322454701244E-4 0.0 0.0 7.627717656923341E-4 5.650161227350622E-5 30-31 1.1300322454701244E-4 0.0 0.0 0.00217531207252999 5.650161227350622E-5 32-33 1.1300322454701244E-4 0.0 0.0 0.004887389461658289 5.650161227350622E-5 34-35 1.1300322454701244E-4 0.0 0.0 0.0077972224937438595 5.650161227350622E-5 36-37 1.1300322454701244E-4 0.0 0.0 0.013249628078137208 5.650161227350622E-5 38-39 1.1300322454701244E-4 0.0 0.0 0.024069686828513653 5.650161227350622E-5 40-41 1.1300322454701244E-4 0.0 0.0 0.03884485843803553 5.650161227350622E-5 42-43 1.1300322454701244E-4 0.0 0.0 0.05251824860822404 5.650161227350622E-5 44-45 1.1300322454701244E-4 0.0 0.0 0.0671804169931989 5.650161227350622E-5 46-47 1.1300322454701244E-4 0.0 0.0 0.08752099741166114 5.650161227350622E-5 48-49 1.1300322454701244E-4 0.0 0.0 0.11020639473947388 5.650161227350622E-5 50-51 1.1300322454701244E-4 0.0 0.0 0.13614063477301325 5.650161227350622E-5 52-53 1.1300322454701244E-4 0.0 0.0 0.16235738286792012 5.650161227350622E-5 54-55 1.1300322454701244E-4 0.0 0.0 0.18902614386101507 5.650161227350622E-5 56-57 1.1300322454701244E-4 0.0 0.0 0.22049754189735804 5.650161227350622E-5 58-59 1.1300322454701244E-4 0.0 0.0 0.2577603551917354 5.650161227350622E-5 60-61 1.1300322454701244E-4 0.0 0.0 0.29858277005934364 5.650161227350622E-5 62-63 1.1300322454701244E-4 0.0 0.0 0.33994195024355023 5.650161227350622E-5 64-65 1.1300322454701244E-4 0.0 0.0 0.38339169008187646 5.650161227350622E-5 66-67 1.6950483682051868E-4 0.0 0.0 0.4336498741991603 5.650161227350622E-5 68-69 1.6950483682051868E-4 0.0 0.0 0.49362633562748714 5.650161227350622E-5 70-71 1.6950483682051868E-4 0.0 0.0 0.561145762294327 5.650161227350622E-5 72-73 2.2600644909402488E-4 0.0 0.0 0.6339480897087398 5.650161227350622E-5 74-75 2.825080613675311E-4 0.0 0.0 0.7017782752430841 5.650161227350622E-5 76-77 3.1075886750428424E-4 0.0 0.0 0.7798917542112064 5.650161227350622E-5 78-79 3.3900967364103735E-4 0.0 0.0 0.873995189452731 5.650161227350622E-5 80-81 4.5201289818804977E-4 0.0 0.0 0.9837495712940169 5.650161227350622E-5 82-83 4.5201289818804977E-4 0.0 0.0 1.099775632097662 5.650161227350622E-5 84-85 4.5201289818804977E-4 0.0 0.0 1.217185982402008 5.650161227350622E-5 86-87 4.5201289818804977E-4 0.0 0.0 1.3453316390383199 5.650161227350622E-5 88-89 4.802637043248029E-4 0.0 0.0 1.4939308793176413 5.650161227350622E-5 90-91 5.650161227350622E-4 0.0 0.0 1.6658370346597842 5.650161227350622E-5 92-93 5.932669288718154E-4 0.0 0.0 1.8511340721107477 5.650161227350622E-5 94-95 6.215177350085685E-4 0.0 0.0 2.028888144323198 5.650161227350622E-5 96-97 6.215177350085685E-4 0.0 0.0 2.2140156769373416 5.650161227350622E-5 98-99 7.627717656923341E-4 0.0 0.0 2.4215460988179296 5.650161227350622E-5 100-101 8.192733779658403E-4 0.0 0.0 2.6686559000961094 5.650161227350622E-5 102-103 8.475241841025934E-4 0.0 0.0 2.9385358511205117 5.650161227350622E-5 104-105 0.001017029020923112 0.0 0.0 3.2112126319524528 5.650161227350622E-5 106-107 0.0010735306331966182 0.0 0.0 3.479764795088428 5.650161227350622E-5 108-109 0.0010735306331966182 0.0 0.0 3.7691660531533264 5.650161227350622E-5 110-111 0.0011300322454701245 0.0 0.0 4.100152497851527 5.650161227350622E-5 112-113 0.0011300322454701245 0.0 0.0 4.472469871927796 5.650161227350622E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3990 0.0 50.0143 1 GTATCAA 6045 0.0 45.822517 1 ATCAACG 7235 0.0 37.91025 3 TCAACGC 7420 0.0 37.127518 4 CAACGCA 7430 0.0 36.711372 5 TATCAAC 7670 0.0 36.091892 2 AACGCAG 7640 0.0 35.773163 6 GTACATG 6530 0.0 31.837175 1 ACGCAGA 9010 0.0 30.26765 7 TACATGG 6540 0.0 30.221245 2 CGCAGAG 9055 0.0 29.919956 8 ACATGGG 6550 0.0 28.067999 3 GCAGAGT 10200 0.0 25.627995 9 GAGTACT 6115 0.0 24.34755 12-13 CGTCTAG 125 0.0016228457 23.827707 1 TACCTGG 1605 0.0 23.367786 2 GTGGTAT 2005 0.0 22.876978 1 CAGAGTA 9985 0.0 22.554201 10-11 GTACTTT 6565 0.0 22.406496 14-15 AGTACTT 6575 0.0 22.055399 12-13 >>END_MODULE