FastQCFastQC Report
Thu 26 May 2016
SRR1364606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405651
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87400.044.1631131
GGTATCA59300.037.2662161
TCAACGC106450.035.756194
ATCAACG106050.035.6663933
GTACATG141050.035.5155871
CAACGCA109250.034.8408165
TACATGG146900.033.574122
AACGCAG110100.033.40916
TATCAAC116550.033.1687052
ACATGGG151700.030.7448433
ACGCAGA130200.027.1050247
CGCAGAG128350.026.2410938
CATGGGA90250.025.0802864
CATGGGG67250.023.3834064
GCAGAGT140950.022.2478339
AGTACAT106950.021.6096172
GGACCGA8400.021.2571756
GTATAGG11550.021.1445261
GAGTACA116950.021.0860981
AGAGTAC132550.019.56902110-11
Produced by FastQC (version 0.11.5)