FastQCFastQC Report
Thu 26 May 2016
SRR1364606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405651
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92520.27166612198372647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.24045329365809942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51220.15039709001304008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81950.052.2070961
GTATCAA131850.047.0914041
ATCAACG162500.037.7498053
TCAACGC162900.037.332774
TATCAAC168700.036.5292662
CAACGCA166300.036.10625
AACGCAG169800.035.2123226
GTACATG134400.031.1680831
ACGCAGA201050.029.5914617
CGCAGAG201950.029.2236718
TACATGG142150.028.6919042
ACATGGG147950.026.3814263
GCAGAGT218500.025.921479
GAGTACT131250.023.86699912-13
GTACTTT135200.023.19172514-15
AGTACTT139300.022.20986212-13
CAGAGTA215400.022.14436710-11
AGAGTAC205550.021.88654110-11
GTGGTAT47250.021.6910551
CATGGGG66700.021.3222244
Produced by FastQC (version 0.11.5)