##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364606_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3405651 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.491070870150818 31.0 30.0 33.0 26.0 34.0 2 29.669085000195263 31.0 30.0 34.0 26.0 34.0 3 29.677986382045606 31.0 30.0 34.0 26.0 34.0 4 33.32267516548231 35.0 33.0 37.0 28.0 37.0 5 32.713658856999736 35.0 33.0 37.0 26.0 37.0 6 32.69199192753456 35.0 33.0 37.0 25.0 37.0 7 32.477928595736905 35.0 33.0 37.0 24.0 37.0 8 32.40785535570145 35.0 33.0 37.0 23.0 37.0 9 33.69696777503038 38.0 33.0 39.0 22.0 39.0 10-11 33.49475048970079 37.0 33.0 39.0 20.5 39.0 12-13 33.352354366316455 37.0 33.0 39.0 18.5 39.0 14-15 34.324708697397355 38.0 33.0 41.0 18.5 41.0 16-17 34.241205279108165 38.0 33.0 41.0 17.5 41.0 18-19 34.283488531267594 38.5 33.0 41.0 18.0 41.0 20-21 34.3076573318875 39.0 33.0 41.0 18.0 41.0 22-23 34.301882371388025 39.0 33.0 41.0 18.0 41.0 24-25 34.2911800122796 39.0 33.0 41.0 18.5 41.0 26-27 34.19364344731741 39.0 33.0 41.0 18.5 41.0 28-29 33.96539868589001 38.5 33.0 41.0 17.0 41.0 30-31 33.90404037289787 38.0 33.0 41.0 17.0 41.0 32-33 33.72878239725679 38.0 33.0 41.0 16.0 41.0 34-35 33.59698997342946 38.0 33.0 40.0 16.0 41.0 36-37 33.4299802005549 38.0 32.0 40.0 15.0 41.0 38-39 33.29192406972999 38.0 32.0 40.0 11.0 41.0 40-41 33.115573057838276 38.0 32.0 40.0 9.0 41.0 42-43 32.93497542760547 38.0 32.0 40.0 8.5 41.0 44-45 32.7289769268783 38.0 31.5 40.0 4.5 41.0 46-47 32.46713506463228 38.0 31.0 40.0 2.0 41.0 48-49 32.20568828103643 38.0 31.0 40.0 2.0 41.0 50-51 32.00496630453326 38.0 30.0 40.0 2.0 41.0 52-53 31.838461280970954 37.5 30.0 40.0 2.0 41.0 54-55 31.622106023194977 37.0 30.0 40.0 2.0 41.0 56-57 31.394000442206206 37.0 30.0 40.0 2.0 41.0 58-59 31.206311363084474 36.5 29.5 40.0 2.0 41.0 60-61 30.98527065750425 36.0 29.0 40.0 2.0 41.0 62-63 30.71743772336038 36.0 29.0 39.5 2.0 41.0 64-65 30.44572476745268 35.0 29.0 39.0 2.0 41.0 66-67 30.166821409475016 35.0 28.5 39.0 2.0 41.0 68-69 29.846668962850273 35.0 28.0 38.5 2.0 40.0 70-71 29.591061885084528 35.0 28.0 38.0 2.0 40.0 72-73 29.432055868320035 35.0 28.0 37.0 2.0 40.0 74-75 29.149570669454974 35.0 28.0 37.0 2.0 39.0 76-77 28.85389254506701 34.0 28.0 36.0 2.0 39.0 78-79 28.554115204405853 34.0 28.0 36.0 2.0 38.5 80-81 28.284355619527663 34.0 27.5 35.5 2.0 37.0 82-83 28.030498427466583 34.0 27.0 35.0 2.0 37.0 84-85 28.012563236808468 34.0 27.5 35.0 2.0 36.5 86-87 27.870042467651558 34.0 27.0 35.0 2.0 36.0 88-89 27.730741200434217 34.0 27.0 35.0 2.0 36.0 90-91 27.579760962001096 34.0 27.0 35.0 2.0 36.0 92-93 27.451010541009634 34.0 27.0 35.0 2.0 35.0 94-95 27.36517952074361 34.0 27.0 35.0 2.0 35.0 96-97 27.28139377757733 34.0 26.5 35.0 2.0 35.0 98-99 27.19468509838501 34.0 26.0 35.0 2.0 35.0 100-101 27.10149718805597 34.0 26.0 35.0 2.0 35.0 102-103 25.931553761674344 32.0 24.0 34.0 2.0 35.0 104-105 26.854265601495868 33.5 25.0 35.0 2.0 35.0 106-107 27.024509557790857 34.0 26.0 35.0 2.0 35.0 108-109 27.0380278249298 34.0 26.0 35.0 2.0 35.0 110-111 26.97600634944685 34.0 25.5 35.0 2.0 35.0 112-113 26.92707150556531 34.0 25.0 35.0 2.0 35.0 114-115 26.854227136015993 34.0 25.0 35.0 2.0 35.0 116-117 26.73925308259713 34.0 25.0 35.0 2.0 35.0 118-119 26.63757120151184 34.0 25.0 35.0 2.0 35.0 120-121 26.50475151446816 34.0 25.0 35.0 2.0 35.0 122-123 26.324718827619154 34.0 25.0 35.0 2.0 35.0 124-125 25.52495352577231 33.0 21.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6810150991905068 1101 2 0.7418825373519198 1101 3 0.6524796981299161 1101 4 0.6511826037673387 1101 5 0.9445198933699501 1101 6 0.9858571151970992 1101 7 0.9515183443470931 1101 8 1.0120526704154003 1101 9 1.2406974998457727 1101 10-11 1.2578752075472508 1101 12-13 1.276765846974662 1101 14-15 1.3599399919267299 1101 16-17 1.4339177046141671 1101 18-19 1.4329421602951413 1101 20-21 1.3902732005715492 1101 22-23 1.2744724232774374 1101 24-25 1.228468028693122 1101 26-27 1.3177093705076288 1101 28-29 1.3373504860620784 1101 30-31 1.3198057534912522 1101 32-33 1.344710153465634 1101 34-35 1.2963731426062353 1101 36-37 1.3606518146743412 1101 38-39 1.3883229933348602 1101 40-41 1.4488584840308505 1101 42-43 1.3444287466908875 1101 44-45 1.3972844318777433 1101 46-47 1.383511125169072 1101 48-49 1.4644493126461953 1101 50-51 1.3960340891426597 1101 52-53 1.3514235953937437 1101 54-55 1.3047185358889593 1101 56-57 1.347072675974534 1101 58-59 1.3367011220777094 1101 60-61 1.3114499334111898 1101 62-63 1.2423215718499918 1101 64-65 1.1676264473455653 1101 66-67 1.191079967160583 1101 68-69 1.2466318768273545 1101 70-71 1.2906112764591562 1101 72-73 1.288404149695122 1101 74-75 1.1680282810055118 1101 76-77 1.2233773709042524 1101 78-79 1.2162017712150544 1101 80-81 1.1727660705244425 1101 82-83 1.1273872399715827 1101 84-85 1.2340927899520047 1101 86-87 1.2069992829071055 1101 88-89 1.1582757823098824 1101 90-91 1.260369199640703 1101 92-93 1.2230099545378472 1101 94-95 1.1620664329293504 1101 96-97 1.2387623455805858 1101 98-99 1.2143141952524736 1101 100-101 1.2656064059169836 1101 102-103 1.234111688243356 1101 104-105 1.2172510006635058 1101 106-107 1.1962131292407427 1101 108-109 1.1737043156458 1101 110-111 1.190594342655487 1101 112-113 1.170035879558128 1101 114-115 1.141814178868806 1101 116-117 1.2534496515484257 1101 118-119 1.2230858530345152 1101 120-121 1.2310548752958965 1101 122-123 1.2855799080470192 1101 124-125 1.2123627870604068 1102 1 0.40582480286674283 1102 2 -0.5863240907409875 1102 3 0.03128256650459349 1102 4 0.2535377123347189 1102 5 0.6535602435300305 1102 6 0.5583747827720771 1102 7 0.6198481596048282 1102 8 0.5261398624303055 1102 9 0.8511991135607957 1102 10-11 0.952605021265633 1102 12-13 0.946574408501867 1102 14-15 1.0547352283818228 1102 16-17 1.125309721775814 1102 18-19 1.0246007888531565 1102 20-21 1.0736608283623568 1102 22-23 1.0204795059758993 1102 24-25 1.0500585763721446 1102 26-27 1.0426192699297872 1102 28-29 1.0574360084227763 1102 30-31 0.9990878296680563 1102 32-33 0.9625509337664653 1102 34-35 0.9275553507005654 1102 36-37 0.8340501877373256 1102 38-39 0.7932835486474517 1102 40-41 0.7646254460436097 1102 42-43 0.63772286120102 1102 44-45 0.553637763035816 1102 46-47 0.4740555055621556 1102 48-49 0.6027954478530049 1102 50-51 0.5699090311074713 1102 52-53 0.6396809838335393 1102 54-55 0.7165548009214078 1102 56-57 0.49084147642115994 1102 58-59 0.612076003980043 1102 60-61 0.5373571172400133 1102 62-63 0.69008454032695 1102 64-65 0.6729922112975828 1102 66-67 0.5491282969703448 1102 68-69 0.44059104919125147 1102 70-71 0.39410248658461455 1102 72-73 0.48031473825510673 1102 74-75 0.577088752780412 1102 76-77 0.6210364189374609 1102 78-79 0.49436632809008785 1102 80-81 0.4553731885731729 1102 82-83 0.4417132524640266 1102 84-85 0.4887436455741998 1102 86-87 0.49239538871491106 1102 88-89 0.5895929041822718 1102 90-91 0.5063299641411199 1102 92-93 0.620260385352065 1102 94-95 0.42160942487603137 1102 96-97 0.27057456363401755 1102 98-99 0.35413412879066897 1102 100-101 0.25926782236321344 1102 102-103 0.28842259303155515 1102 104-105 0.22461284529880388 1102 106-107 0.530117033136392 1102 108-109 0.6068841987590119 1102 110-111 0.42749873117418247 1102 112-113 0.4691215555797861 1102 114-115 0.6741078587850424 1102 116-117 0.5996699354006374 1102 118-119 0.6325785821769578 1102 120-121 0.6090035371397882 1102 122-123 0.6293045069135559 1102 124-125 0.5764651939848804 1103 1 0.22838719517764616 1103 2 -0.969705879390176 1103 3 0.010437918165884241 1103 4 0.2495165879684791 1103 5 0.5260141055339247 1103 6 0.6844839956992743 1103 7 0.6082219554306221 1103 8 0.7076164657949064 1103 9 0.7939514060847657 1103 10-11 0.9477804658633175 1103 12-13 0.971603256903812 1103 14-15 1.0317087843890036 1103 16-17 1.10427327599308 1103 18-19 1.1223720490991838 1103 20-21 1.1131053409987928 1103 22-23 1.0594403364141556 1103 24-25 0.8664467305184118 1103 26-27 0.8660758260734411 1103 28-29 1.0040345417866519 1103 30-31 1.0120380265975228 1103 32-33 0.9723269188994479 1103 34-35 0.9942884350775145 1103 36-37 1.0380071828250692 1103 38-39 1.0726730264274522 1103 40-41 1.0419236415639546 1103 42-43 1.0539316919175477 1103 44-45 1.0192387438515667 1103 46-47 1.1377721163816474 1103 48-49 1.0965317733594304 1103 50-51 1.0999721435563359 1103 52-53 1.0259502255590753 1103 54-55 1.0443215737933897 1103 56-57 1.0553930044919078 1103 58-59 1.0446029556496832 1103 60-61 0.9949003914385912 1103 62-63 0.9990550440102552 1103 64-65 1.0098529075933094 1103 66-67 0.8635346306261731 1103 68-69 0.8079416950097063 1103 70-71 0.9251934785551725 1103 72-73 0.9293382646769004 1103 74-75 0.8897304542120281 1103 76-77 0.821767303627503 1103 78-79 0.8171606285625614 1103 80-81 0.9052923149150729 1103 82-83 0.9162692152522922 1103 84-85 0.9399343674286911 1103 86-87 0.9400102730103512 1103 88-89 0.9429272776151656 1103 90-91 1.03704739388942 1103 92-93 0.9884740199403588 1103 94-95 0.8713754682954153 1103 96-97 0.8710401630989644 1103 98-99 0.9341929868307588 1103 100-101 0.9178668361780957 1103 102-103 0.9694300146910955 1103 104-105 0.9340432280324364 1103 106-107 0.8988382647663933 1103 108-109 0.910359175116426 1103 110-111 0.976709914321475 1103 112-113 0.9615116090313585 1103 114-115 0.9128801189866778 1103 116-117 0.8748486455013911 1103 118-119 0.8911631234226469 1103 120-121 0.8703209526149784 1103 122-123 0.936748010937972 1103 124-125 0.9174933359914199 1104 1 0.30795181066231336 1104 2 -0.7203404233578077 1104 3 0.1050009942122685 1104 4 0.23888689407792896 1104 5 0.595673814365064 1104 6 0.5978721349295668 1104 7 0.657918082261503 1104 8 0.752579700443718 1104 9 0.7788765865820082 1104 10-11 0.8936853712714381 1104 12-13 0.8055289817601334 1104 14-15 0.8970605529449784 1104 16-17 1.0836535597486616 1104 18-19 0.9623397821925437 1104 20-21 1.0074471395756532 1104 22-23 0.9487460838021988 1104 24-25 1.0082619607956858 1104 26-27 0.884146255092773 1104 28-29 1.189754976135923 1104 30-31 0.9886490533345551 1104 32-33 1.1043672706689804 1104 34-35 1.0178088764866828 1104 36-37 1.0053494912010095 1104 38-39 1.0011760716586053 1104 40-41 0.9896565302043996 1104 42-43 0.9986374467079813 1104 44-45 0.9908061885867809 1104 46-47 0.9933597091356745 1104 48-49 0.9782062774553992 1104 50-51 0.9822686305352164 1104 52-53 0.7596786976014833 1104 54-55 0.9385605290972094 1104 56-57 1.0402413979398695 1104 58-59 1.001982562760741 1104 60-61 0.9720130442669088 1104 62-63 0.9532009550492901 1104 64-65 0.9913972784623795 1104 66-67 0.9390576903452867 1104 68-69 0.9400605657209056 1104 70-71 1.0015757125772673 1104 72-73 0.9480851047030114 1104 74-75 1.0136494176826005 1104 76-77 0.9374145544312498 1104 78-79 0.932546130101283 1104 80-81 0.8808542429697717 1104 82-83 0.932366318294477 1104 84-85 0.8373586036553888 1104 86-87 0.7801742742686812 1104 88-89 0.8357234108793428 1104 90-91 0.9206013320434252 1104 92-93 0.8662372883085254 1104 94-95 0.7859500511126996 1104 96-97 0.8081302227099236 1104 98-99 0.8544575561614707 1104 100-101 0.8826178248843277 1104 102-103 0.8574114871008653 1104 104-105 0.8409014129383756 1104 106-107 0.8135421557292482 1104 108-109 0.8309706872503533 1104 110-111 0.6967951218704869 1104 112-113 0.8512938214223134 1104 114-115 0.847448095704749 1104 116-117 0.8882643704694075 1104 118-119 0.777671475405608 1104 120-121 0.7802318216486164 1104 122-123 0.8668021506725765 1104 124-125 0.8102029524737766 1105 1 0.42634844386077475 1105 2 0.2088750715722263 1105 3 0.38502984484977176 1105 4 0.3927052161801896 1105 5 0.5255329021679458 1105 6 0.4747735848504959 1105 7 0.6166851073715094 1105 8 0.5787355095406994 1105 9 0.5504248605602982 1105 10-11 0.6820156336974179 1105 12-13 0.7087895577025378 1105 14-15 0.7405094048011378 1105 16-17 0.8578627728602157 1105 18-19 0.8886203288386412 1105 20-21 0.8875084675910685 1105 22-23 0.819430973353839 1105 24-25 0.8714052788729205 1105 26-27 0.8399438958614382 1105 28-29 0.9348173904132651 1105 30-31 0.8377880002233695 1105 32-33 0.8574557019026443 1105 34-35 0.8927103573420183 1105 36-37 0.9342325162021083 1105 38-39 0.8611322474218852 1105 40-41 0.7974284281569695 1105 42-43 0.8450728541206161 1105 44-45 0.8608751988952434 1105 46-47 0.8240566398848941 1105 48-49 0.7869356813131247 1105 50-51 0.7154383773034212 1105 52-53 0.6913383099634594 1105 54-55 0.6611466092226337 1105 56-57 0.6301845411815528 1105 58-59 0.6246268340183043 1105 60-61 0.6764390382583656 1105 62-63 0.6558604111288595 1105 64-65 0.6062787373437999 1105 66-67 0.5961069160807781 1105 68-69 0.5697035209181145 1105 70-71 0.6322444091299886 1105 72-73 0.549696135446677 1105 74-75 0.5810176935836182 1105 76-77 0.6435689051920974 1105 78-79 0.6247550760384684 1105 80-81 0.4526364574333428 1105 82-83 0.515216214197963 1105 84-85 0.45076195235318295 1105 86-87 0.54851256091397 1105 88-89 0.6035477033213503 1105 90-91 0.5128549493555745 1105 92-93 0.39779000470675285 1105 94-95 0.5595875020834491 1105 96-97 0.5126146025779477 1105 98-99 0.680072631147624 1105 100-101 0.6478221681493892 1105 102-103 0.18354501294155767 1105 104-105 0.11546478002154359 1105 106-107 0.5697837426296566 1105 108-109 0.530111355005765 1105 110-111 0.4030585482346041 1105 112-113 0.2944839141142914 1105 114-115 0.44208761310110134 1105 116-117 0.16959085564001342 1105 118-119 0.0806609184226943 1105 120-121 0.06572793475587702 1105 122-123 0.15341208953667973 1105 124-125 0.0911789913070109 1106 1 0.47605412214865694 1106 2 0.5238719253503028 1106 3 0.6053918214516294 1106 4 0.3729430808986862 1106 5 0.5722396631119508 1106 6 0.6050697657677091 1106 7 0.6278720839977723 1106 8 0.7184381401351274 1106 9 0.7121128625877091 1106 10-11 0.79877675089962 1106 12-13 0.6779637058136458 1106 14-15 0.8741661838978843 1106 16-17 0.9653128774106818 1106 18-19 0.9464460727258768 1106 20-21 0.9809415686225975 1106 22-23 1.0036466872815382 1106 24-25 1.0483664952833394 1106 26-27 1.0320070136688386 1106 28-29 1.1940118050169417 1106 30-31 1.0548641156533165 1106 32-33 0.8319000022404097 1106 34-35 0.8910555310252875 1106 36-37 0.9691438646244066 1106 38-39 0.9896207292268997 1106 40-41 1.03295899817833 1106 42-43 0.9694204103488246 1106 44-45 0.902814917478409 1106 46-47 0.7014282517755746 1106 48-49 0.8187278895805434 1106 50-51 0.9781474560709142 1106 52-53 1.0384759458464181 1106 54-55 1.049918236176424 1106 56-57 0.925763639276159 1106 58-59 0.8596502626419884 1106 60-61 0.906140718279044 1106 62-63 0.9494956419590075 1106 64-65 0.9171689426051799 1106 66-67 0.7802039619586338 1106 68-69 0.8276973706445183 1106 70-71 0.8748376263723188 1106 72-73 0.8716138820004318 1106 74-75 0.9915625393858392 1106 76-77 0.9428153052239736 1106 78-79 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2216 1 -1.240588526366274 2216 2 -0.984678998293397 2216 3 -0.7792630628130333 2216 4 -0.9432483028838661 2216 5 -1.4703257797568874 2216 6 -1.5188269558159746 2216 7 -1.3261849802382066 2216 8 -1.4998559211778826 2216 9 -2.1489889061097145 2216 10-11 -1.7103952859683602 2216 12-13 -1.5592077938863937 2216 14-15 -1.857353335546243 2216 16-17 -1.7457838907266563 2216 18-19 -2.368183130626914 2216 20-21 -2.3585223740645773 2216 22-23 -2.325323088869375 2216 24-25 -2.512727318907853 2216 26-27 -2.184643821301904 2216 28-29 -3.749501070026767 2216 30-31 -3.3105870317398605 2216 32-33 -2.5207181764123376 2216 34-35 -2.1360825358714273 2216 36-37 -2.107768859843091 2216 38-39 -2.1769309234206915 2216 40-41 -2.430637436019822 2216 42-43 -2.6593844496498207 2216 44-45 -2.6974567671127296 2216 46-47 -2.2332369235462437 2216 48-49 -2.4413250952080645 2216 50-51 -2.689059709970465 2216 52-53 -2.453687893377591 2216 54-55 -2.1710300958263815 2216 56-57 -2.2106631010176656 2216 58-59 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-2.143182421156464 2216 124-125 -2.24245509653883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 141177.0 3 43953.0 4 20050.0 5 15376.0 6 16440.0 7 19510.0 8 21741.0 9 23372.0 10 25324.0 11 28132.0 12 31280.0 13 34178.0 14 37254.0 15 31543.0 16 20626.0 17 17688.0 18 16235.0 19 15151.0 20 14856.0 21 15384.0 22 18566.0 23 24088.0 24 33723.0 25 44604.0 26 52871.0 27 59307.0 28 68104.0 29 80157.0 30 92454.0 31 108798.0 32 133076.0 33 163879.0 34 210611.0 35 285857.0 36 456679.0 37 709232.0 38 273409.0 39 966.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8923671350542 19.672129702617443 13.857129803978832 24.57837335834952 2 15.600141287846952 20.684329214788676 38.81004953890441 24.90547995845996 3 19.338710866145707 24.857420798549235 29.26841299945297 26.535455335852088 4 12.929218892972633 17.137775256271564 36.34906847181453 33.58393737894127 5 13.059233289823213 38.81747837907379 33.9998977845086 14.123390546594402 6 31.977395124612517 37.47739409690463 16.743093876417245 13.802116902065606 7 28.2975305426546 32.973940047221575 20.361417375318112 18.36711203480572 8 26.17292621758731 36.43619762341921 18.837496171801615 18.553379987191867 9 26.105302102694655 16.47507215992342 19.624269224783667 37.795356512598254 10-11 24.80627989829658 27.41150456778103 26.814890137623976 20.967325396298413 12-13 25.660425895176125 24.468291760410875 27.737880255946468 22.133402088466536 14-15 22.57443963524333 25.85806897205859 25.817897403046082 25.749593989652002 16-17 21.97363859640876 29.125032316442606 26.431251175143366 22.470077912005266 18-19 21.965401849782033 27.516039915499107 28.989843185335406 21.528715049383457 20-21 22.609207386665222 26.56695147282977 29.275274986906435 21.548566153598575 22-23 22.37789571491165 26.379462140887888 29.16885449447375 22.07378764972671 24-25 22.10550934314761 26.568136312264528 29.3063939904588 22.019960354129065 26-27 21.89139462616692 26.854014107728595 29.295177339075558 21.95941392702893 28-29 21.82390447072248 26.78470335269666 29.339099733038136 22.052292443542722 30-31 22.041387106719714 26.5684377431621 29.325620659785944 22.06455449033224 32-33 21.920838142914068 26.59908343167831 29.23986258816178 22.240215837245845 34-35 22.23963935235877 26.598013125831155 29.201201180038705 21.961146341771368 36-37 22.273991021053792 26.435287296768724 29.051188273782248 22.239533408395236 38-39 22.027697494546565 26.628682739364663 29.174613018186534 22.169006747902237 40-41 22.194177322977993 26.567189816921882 29.086708851467414 22.151924008632715 42-43 22.0836768297562 26.469541930544104 28.921295212143143 22.52548602755655 44-45 22.24949062602128 26.696995669844032 28.734785214339343 22.31872848979534 46-47 22.455808291205965 26.997738607185806 28.195837821592946 22.350615280015283 48-49 22.487428185177855 27.008554060712335 27.961856735045515 22.54216101906429 50-51 22.50583153043319 27.161028346053467 27.83834915422255 22.494790969290797 52-53 22.62222791212005 27.184827783040284 27.66074336557587 22.5322009392638 54-55 22.527543192182637 27.18073578296778 27.66926205885453 22.622458965995047 56-57 22.420294617087933 27.38248453343975 27.64302806018253 22.55419278928979 58-59 22.608133159876505 27.079554992504367 27.603052632676196 22.709259214942932 60-61 22.511848689134617 27.197296493387018 27.807752467883528 22.483102349594837 62-63 22.40912236750037 27.173644627708477 28.01276466672598 22.404468338065175 64-65 22.342087834533483 27.055547456197033 27.85212793149675 22.750236777772738 66-67 22.32477725991301 27.23062345495766 27.742507966905595 22.70209131822374 68-69 22.274245547190162 27.067528318177793 27.951402831281904 22.70682330335014 70-71 22.336522444607507 26.96543186603677 27.896634153059136 22.801411536296584 72-73 22.43933715142133 27.068744376816156 27.597353022013515 22.894565449748995 74-75 22.298717592018225 27.37903234686754 27.565759288446728 22.756490772667508 76-77 22.507334908762896 27.151788566703626 27.710245851577618 22.630630672955856 78-79 22.481124112730157 27.098443549546243 27.637511317751063 22.782921019972534 80-81 22.293962063869945 27.284767628540408 27.666360552532343 22.754909755057305 82-83 22.539651079310968 27.23633849094665 27.58245501219299 22.64155541754939 84-85 22.38402228536252 27.143445871536464 27.594755587306775 22.877776255794245 86-87 22.310437311415104 27.17406305092629 27.709603045312054 22.80589659234655 88-89 22.51917474808781 27.148627384309197 27.59589869895653 22.736299168646465 90-91 22.3993445012422 27.259913009289562 27.683062650870564 22.657679838597673 92-93 22.352632493912544 27.14298677028359 27.694161329009177 22.810219406794694 94-95 22.262613715782486 27.06382766296081 27.699622953996865 22.97393566725984 96-97 22.473832373020443 27.084861237319956 27.52480949010256 22.916496899557043 98-99 22.24987113135294 27.134855405046892 27.40952070259583 23.20575276100434 100-101 22.170632864634435 27.07183553368265 27.647514038137107 23.110017563545814 102-103 22.21486026439624 26.867237522631537 27.684483354993443 23.23341885797878 104-105 22.14958424677284 26.868716647101003 27.723155742879573 23.25854336324658 106-107 22.145814350454458 27.00376246409832 27.60510189309528 23.245321292351946 108-109 22.17437030774677 26.983947764346382 27.363226227886862 23.478455700019982 110-111 22.120623564960358 27.068105096544336 27.438640608301917 23.37263073019339 112-113 22.084253468962096 26.97097933458943 27.61782593356113 23.326941262887342 114-115 22.007327872371228 27.080989785434518 27.36487290005629 23.546809442137967 116-117 21.870666555280238 27.107791182158948 27.505992770942473 23.515549491618344 118-119 21.84868224875501 27.03133159920606 27.420372388586827 23.699613763452103 120-121 21.77869970083998 27.13212817686827 27.51260498278956 23.576567139502192 122-123 21.732674343011276 27.110500485375034 27.41577583366758 23.74104933794611 124-125 21.71081763266725 27.038781555490566 27.36991380049495 23.88048701134723 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 176.0 1 164.0 2 226.5 3 360.0 4 346.0 5 365.0 6 865.5 7 1806.5 8 2713.5 9 3194.5 10 2839.5 11 2266.5 12 2298.0 13 2587.0 14 2875.0 15 3189.5 16 3501.5 17 3826.5 18 4263.0 19 4738.5 20 5282.5 21 6056.5 22 7211.5 23 8674.5 24 10430.5 25 12874.5 26 16674.5 27 21684.0 28 26182.0 29 31096.0 30 38567.5 31 46306.5 32 54954.5 33 63822.0 34 71996.0 35 79797.5 36 88827.0 37 96470.5 38 100031.0 39 103005.5 40 104709.5 41 107641.0 42 110475.0 43 113897.5 44 119231.5 45 122872.5 46 123986.5 47 125550.0 48 126912.5 49 125395.5 50 123442.0 51 119369.5 52 115936.5 53 114279.5 54 112140.5 55 106421.5 56 96761.0 57 88413.0 58 81777.0 59 72773.0 60 60110.0 61 48096.5 62 38466.5 63 29833.0 64 22449.0 65 16878.5 66 12144.5 67 8853.0 68 6637.0 69 4659.5 70 3276.5 71 2476.0 72 1937.0 73 1671.0 74 1266.5 75 838.5 76 577.5 77 339.5 78 224.5 79 144.0 80 103.5 81 72.0 82 47.0 83 39.5 84 19.5 85 10.0 86 10.0 87 8.0 88 5.0 89 4.0 90 4.5 91 2.5 92 0.5 93 0.5 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05837356793165242 2 0.07836974487403436 3 0.0 4 0.008192266324411984 5 0.03168263571340692 6 4.110814643074114E-4 7 1.1745184694497468E-4 8 5.872592347248734E-5 9 6.166221964611172E-4 10-11 0.05957744936283842 12-13 0.00967509589209229 14-15 0.007560962647082746 16-17 0.05317632370433729 18-19 1.9085925128558388E-4 20-21 0.010350444012025894 22-23 0.013125243896100923 24-25 0.0 26-27 0.0 28-29 0.05267715335482115 30-31 2.936296173624367E-5 32-33 0.003582281331821728 34-35 0.0 36-37 5.138518303842642E-4 38-39 0.0 40-41 2.936296173624367E-5 42-43 0.003185881348382438 44-45 0.0 46-47 1.9085925128558388E-4 48-49 4.991703495161424E-4 50-51 7.927999668785792E-4 52-53 1.4681480868121836E-4 54-55 0.0 56-57 0.0022903110154270066 58-59 1.4681480868121835E-5 60-61 0.0 62-63 0.0 64-65 9.249332946916756E-4 66-67 0.0 68-69 6.019407155929953E-4 70-71 0.0 72-73 0.003141836905778073 74-75 0.004213585009150967 76-77 5.578962729886299E-4 78-79 0.001453466605944062 80-81 0.0017177332615702548 82-83 1.7617777041746202E-4 84-85 2.2022221302182756E-4 86-87 6.753481199336045E-4 88-89 0.0 90-91 0.0 92-93 0.008427170018301934 94-95 0.006782844161072289 96-97 0.00735542191492904 98-99 0.001512192529416549 100-101 5.138518303842642E-4 102-103 1.4681480868121836E-4 104-105 7.340740434060918E-4 106-107 0.004991703495161424 108-109 0.006973703412357872 110-111 0.001159836988581625 112-113 0.0038025035448435557 114-115 7.047110816698481E-4 116-117 0.0026279850753938086 118-119 0.0028041628458112706 120-121 0.013213332781309654 122-123 0.001086429584241016 124-125 0.0018498665893833513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3405651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.35503799586755 #Duplication Level Percentage of deduplicated Percentage of total 1 74.34697794274496 37.437448991848484 2 12.548074541208779 12.637175405950924 3 5.057154705865793 7.639596520945571 4 2.5043517343335564 5.044269069495321 5 1.4680162524013365 3.6961007084110187 6 0.9426863459876715 2.848140406223685 7 0.5954288335973738 2.0987989077741593 8 0.4340276648849696 1.7484383645232249 9 0.3202112971754449 1.4511826832378 >10 1.6041188523515018 14.470373774032078 >50 0.11729346776818186 4.024464361552719 >100 0.05860191155674213 5.182080045861392 >500 0.002762560689454397 0.9144482936446631 >1k 1.1755577365449268E-4 0.13967120296928673 >5k 1.76333660481739E-4 0.6678112635295649 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9252 0.27166612198372647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8189 0.24045329365809942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5122 0.15039709001304008 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.872592347248735E-5 0.0 0.0 0.0 0.0 3 5.872592347248735E-5 0.0 0.0 0.0 0.0 4 5.872592347248735E-5 0.0 0.0 0.0 0.0 5 8.808888520873102E-5 0.0 0.0 0.0 0.0 6 8.808888520873102E-5 0.0 0.0 0.0 0.0 7 1.4681480868121836E-4 0.0 0.0 0.0 0.0 8 1.4681480868121836E-4 0.0 0.0 0.0 0.0 9 1.4681480868121836E-4 0.0 0.0 0.0 0.0 10-11 2.055407321537057E-4 0.0 0.0 2.9362961736243674E-5 0.0 12-13 2.789481364943149E-4 0.0 0.0 2.9362961736243674E-5 0.0 14-15 3.229925790986804E-4 0.0 0.0 4.404444260436551E-5 0.0 16-17 3.229925790986804E-4 0.0 0.0 5.872592347248735E-5 0.0 18-19 4.110814643074114E-4 0.0 0.0 5.872592347248735E-5 0.0 20-21 4.698073877798988E-4 0.0 0.0 8.808888520873102E-5 0.0 22-23 4.698073877798988E-4 0.0 0.0 2.495851747580712E-4 0.0 24-25 6.166221964611172E-4 0.0 0.0 4.551259069117769E-4 0.0 26-27 6.31303677329239E-4 0.0 0.0 7.340740434060918E-4 0.0 28-29 8.662073712191883E-4 0.0 0.0 0.001159836988581625 0.0 30-31 9.102518138235538E-4 0.0 0.0 0.003215244310118682 0.0 32-33 9.249332946916758E-4 0.0 0.0 0.007326058953192797 0.0 34-35 9.983406990322849E-4 0.0 0.0 0.01256734762311229 0.0 36-37 0.0010570666225047723 0.0 0.0 0.02133219170138103 0.0 38-39 0.0011451555077135033 0.0 0.0 0.03676242809377708 0.0 40-41 0.0011451555077135033 0.0 0.0 0.0538516718242709 0.0 42-43 0.0011891999503178688 0.0 0.0 0.06976639708531497 0.0 44-45 0.0013213332781309654 0.0 0.0 0.08918999627384017 0.0 46-47 0.0014975110485484274 0.0 0.0 0.11388424709402109 0.0 48-49 0.001614962895493402 0.0 0.0 0.14150011260695827 0.0 50-51 0.001614962895493402 0.0 0.0 0.17328551868644204 0.0 52-53 0.001614962895493402 0.0 0.0 0.20166482120452156 0.0 54-55 0.001614962895493402 0.0 0.0 0.23452197538737823 0.0 56-57 0.0017324147424383767 0.0 0.0 0.27217997381411074 0.0 58-59 0.0017324147424383767 0.0 0.0 0.31879367557039756 0.0 60-61 0.0017470962233064984 0.0 0.0 0.3674921476099577 0.0 62-63 0.0017617777041746203 0.0 0.0 0.4175853603319894 0.0 64-65 0.0017617777041746203 0.0 0.0 0.46944035075819573 0.0 66-67 0.0019379554745920823 0.0 0.0 0.530324451918297 0.0 68-69 0.0019379554745920823 0.0 0.0 0.6052587302691909 0.0 70-71 0.002055407321537057 0.0 0.0 0.6887082675235954 0.0 72-73 0.002172859168482032 0.0 0.0 0.7741985306186687 0.0 74-75 0.002260948053690763 0.0 0.0 0.8616267491883343 0.0 76-77 0.0022903110154270066 0.0 0.0 0.9589062414205096 0.0 78-79 0.0022903110154270066 0.0 0.0 1.0752863402621116 0.0 80-81 0.002701392479734418 0.0 0.0 1.2104587346149092 0.0 82-83 0.0027307554414706618 0.0 0.0 1.3507402843098133 0.0 84-85 0.002848207288415636 0.0 0.0 1.489891359977872 0.0 86-87 0.002995022097096855 0.0 0.0 1.6413161536516807 0.0 88-89 0.003024385058833098 0.0 0.0 1.816818575949209 0.0 90-91 0.0032005628292505603 0.0 0.0 2.021757367387322 0.0 92-93 0.003318014676195535 0.0 0.0 2.243521135900302 2.9362961736243674E-5 94-95 0.003318014676195535 0.0 0.0 2.4658868451288756 2.9362961736243674E-5 96-97 0.003318014676195535 0.0 0.0 2.692143146787501 4.404444260436551E-5 98-99 0.003685051697898581 0.0 0.0 2.9450169732600315 5.872592347248735E-5 100-101 0.003875910949184165 0.0 0.0 3.2399972868623355 5.872592347248735E-5 102-103 0.0039933627961291395 0.0 0.0 3.563371584463587 5.872592347248735E-5 104-105 0.004286992413491576 0.0 0.0 3.8881406227473105 5.872592347248735E-5 106-107 0.004448488703040916 0.0 0.0 4.212909661031033 5.872592347248735E-5 108-109 0.004521896107381526 0.0 0.0 4.556999528137204 5.872592347248735E-5 110-111 0.004727436839535231 0.0 0.0 4.956702844771822 5.872592347248735E-5 112-113 0.004786162763007719 0.0 0.0 5.392919004325458 5.872592347248735E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8195 0.0 52.207096 1 GTATCAA 13185 0.0 47.091404 1 ATCAACG 16250 0.0 37.749805 3 TCAACGC 16290 0.0 37.33277 4 TATCAAC 16870 0.0 36.529266 2 CAACGCA 16630 0.0 36.1062 5 AACGCAG 16980 0.0 35.212322 6 GTACATG 13440 0.0 31.168083 1 ACGCAGA 20105 0.0 29.591461 7 CGCAGAG 20195 0.0 29.223671 8 TACATGG 14215 0.0 28.691904 2 ACATGGG 14795 0.0 26.381426 3 GCAGAGT 21850 0.0 25.92147 9 GAGTACT 13125 0.0 23.866999 12-13 GTACTTT 13520 0.0 23.191725 14-15 AGTACTT 13930 0.0 22.209862 12-13 CAGAGTA 21540 0.0 22.144367 10-11 AGAGTAC 20555 0.0 21.886541 10-11 GTGGTAT 4725 0.0 21.691055 1 CATGGGG 6670 0.0 21.322224 4 >>END_MODULE