FastQCFastQC Report
Thu 26 May 2016
SRR1364605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011956
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66280.2200563354843165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131850.031.9872631
TACATGG137600.030.0442
ACATGGG133800.029.0719283
CATGGGG55300.023.8062574
CATGGGA90850.022.8180394
GTATCAA136750.022.6080971
GTATAGG9750.022.6058641
CGCAGAT7250.021.3365638
GTCCAAG22650.020.5140021
ATCAACG150750.019.5202963
TCAACGC150350.019.4140724
AGTACAT104350.019.2377452
ATGGGAG39000.019.0931075
AACGCAG151200.018.8952226
TATCAAC159350.018.7658582
CAACGCA155000.018.7166655
GAGTACT40150.018.52063412-13
ATGGGAT40350.017.5684975
TACGGTT2752.8645527E-517.311157
ACGCAGA165350.017.1305527
Produced by FastQC (version 0.11.5)