##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3011956 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.61891475174272 30.0 25.0 31.0 2.0 34.0 2 25.483863642098356 30.0 25.0 31.0 2.0 34.0 3 25.912327404517196 31.0 26.0 31.0 2.0 34.0 4 29.12083742259183 35.0 28.0 37.0 2.0 37.0 5 29.482630556356067 35.0 30.0 37.0 2.0 37.0 6 29.417363002646784 35.0 29.0 37.0 2.0 37.0 7 29.30012556624333 35.0 29.0 37.0 2.0 37.0 8 29.029127583537075 35.0 28.0 37.0 2.0 37.0 9 29.87858819982762 35.0 28.0 39.0 2.0 39.0 10-11 29.805173780759084 35.0 27.5 39.0 2.0 39.0 12-13 29.815899037037724 35.0 27.0 39.0 2.0 39.0 14-15 30.381238138936958 36.5 27.0 40.0 2.0 41.0 16-17 30.750018260558917 37.0 28.0 40.0 2.0 41.0 18-19 30.5260330828206 37.0 27.0 40.0 2.0 41.0 20-21 30.410507158803117 37.0 27.0 40.0 2.0 41.0 22-23 30.449200453127467 37.0 27.0 40.0 2.0 41.0 24-25 30.507370293589943 37.0 27.0 40.0 2.0 41.0 26-27 30.296322887850952 37.0 27.0 40.0 2.0 41.0 28-29 30.180602903893682 37.0 27.0 40.0 2.0 41.0 30-31 30.03324633560384 36.5 27.0 40.0 2.0 41.0 32-33 29.94285739897927 36.0 27.0 40.0 2.0 41.0 34-35 29.989850947357798 36.5 27.0 40.0 2.0 41.0 36-37 29.915530306551624 36.0 27.0 40.0 2.0 41.0 38-39 29.616470658933927 36.0 25.5 40.0 2.0 41.0 40-41 29.47287277768998 36.0 25.0 40.0 2.0 41.0 42-43 28.955816585634054 35.5 24.0 40.0 2.0 41.0 44-45 28.785044502642137 35.0 24.0 40.0 2.0 41.0 46-47 28.729705546827375 35.0 24.0 40.0 2.0 41.0 48-49 28.655627439444665 35.0 24.0 40.0 2.0 41.0 50-51 28.412583384352228 35.0 23.0 39.0 2.0 41.0 52-53 28.283015256530973 35.0 23.0 39.0 2.0 40.5 54-55 28.093122044279532 34.5 22.5 39.0 2.0 40.0 56-57 27.864826046595635 34.0 22.0 39.0 2.0 40.0 58-59 27.642661612586636 34.0 22.0 39.0 2.0 40.0 60-61 27.33570609929229 34.0 20.0 38.0 2.0 40.0 62-63 27.13593126858427 34.0 19.5 38.0 2.0 40.0 64-65 26.90145274366558 33.0 19.5 38.0 2.0 40.0 66-67 26.6448312989964 33.0 18.5 37.5 2.0 40.0 68-69 26.422056962319502 33.0 18.0 37.0 2.0 40.0 70-71 25.996978375514118 32.5 15.5 36.5 2.0 39.0 72-73 25.757761899576224 32.0 14.0 36.0 2.0 39.0 74-75 25.729756676392352 32.0 15.0 36.0 2.0 39.0 76-77 25.495052550568467 32.0 13.0 35.0 2.0 38.0 78-79 25.220222672575563 32.0 11.5 35.0 2.0 37.0 80-81 24.845945292693518 32.0 7.5 35.0 2.0 37.0 82-83 24.985389726808755 32.0 9.0 35.0 2.0 36.0 84-85 24.84359715082159 32.0 7.0 35.0 2.0 36.0 86-87 24.7310541721061 32.0 7.0 35.0 2.0 36.0 88-89 24.573710074117947 32.0 6.0 35.0 2.0 35.5 90-91 24.452407007273678 32.0 2.0 35.0 2.0 35.0 92-93 24.343446584213048 32.0 2.0 35.0 2.0 35.0 94-95 24.248617011669495 32.0 2.0 35.0 2.0 35.0 96-97 24.139052994134047 32.0 2.0 35.0 2.0 35.0 98-99 24.05036793366171 32.0 2.0 35.0 2.0 35.0 100-101 23.946899788708734 32.0 2.0 35.0 2.0 35.0 102-103 22.84861299434653 29.5 2.0 34.0 2.0 35.0 104-105 23.662672695085853 31.5 2.0 34.5 2.0 35.0 106-107 23.822733134215774 32.0 2.0 35.0 2.0 35.0 108-109 23.780865988746186 32.0 2.0 35.0 2.0 35.0 110-111 23.71389339684909 32.0 2.0 35.0 2.0 35.0 112-113 23.625708011670824 32.0 2.0 35.0 2.0 35.0 114-115 23.510967955707187 32.0 2.0 35.0 2.0 35.0 116-117 23.39707120555546 32.0 2.0 35.0 2.0 35.0 118-119 23.266354488578187 32.0 2.0 35.0 2.0 35.0 120-121 23.077616505686006 32.0 2.0 35.0 2.0 35.0 122-123 22.860747467758493 32.0 2.0 35.0 2.0 35.0 124-125 21.91070968500204 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6472362439987016 1101 2 1.874448417924441 1101 3 1.294922971742615 1101 4 1.3022786678318958 1101 5 1.416746031858537 1101 6 1.2941631765524377 1101 7 1.3266704339908735 1101 8 1.4892261838599943 1101 9 1.8414761976985083 1101 10-11 1.831867588982341 1101 12-13 1.8473593974486917 1101 14-15 2.110862908599774 1101 16-17 1.6916393677271486 1101 18-19 1.7922141957004456 1101 20-21 1.9756479205162023 1101 22-23 1.7168443066484471 1101 24-25 1.8637079202528923 1101 26-27 2.026211732173085 1101 28-29 2.0387430105000703 1101 30-31 2.210057917891284 1101 32-33 2.1254211370115677 1101 34-35 1.9217179369803787 1101 36-37 1.9151504336612888 1101 38-39 1.8901573384267678 1101 40-41 2.006922393545615 1101 42-43 2.151480768170188 1101 44-45 1.900121328735743 1101 46-47 1.6882136962351986 1101 48-49 1.5139140449469224 1101 50-51 1.6275933260533328 1101 52-53 1.563688328623602 1101 54-55 1.5018368700638618 1101 56-57 1.4571098047664641 1101 58-59 1.3490801548026 1101 60-61 1.4873919768408754 1101 62-63 1.3357605702198292 1101 64-65 1.350206742675958 1101 66-67 1.3775585223209568 1101 68-69 1.2902598726089032 1101 70-71 1.3237704557488428 1101 72-73 1.170954771122279 1101 74-75 1.1806419965236614 1101 76-77 1.2795033166474639 1101 78-79 1.2477302054373283 1101 80-81 1.3407883348331282 1101 82-83 1.3702137566324986 1101 84-85 1.4352409400498125 1101 86-87 1.4080688485191573 1101 88-89 1.3072201493797166 1101 90-91 1.3638066196131362 1101 92-93 1.3820787729564579 1101 94-95 1.3512037323092656 1101 96-97 1.3334264195775987 1101 98-99 1.4174073445215 1101 100-101 1.4098510779589581 1101 102-103 1.2879557251565572 1101 104-105 1.4332786860096363 1101 106-107 1.4283815142174134 1101 108-109 1.4464565598975128 1101 110-111 1.4946393869022536 1101 112-113 1.4577701927428137 1101 114-115 1.4551004010484867 1101 116-117 1.4429357051765415 1101 118-119 1.3522026056665233 1101 120-121 1.3706146296989594 1101 122-123 1.3271975465336752 1101 124-125 1.1961673137390143 1102 1 0.4607950310326956 1102 2 0.9280461182834863 1102 3 0.6573136791636713 1102 4 0.579509545661189 1102 5 0.5044455808967463 1102 6 0.22370073675902447 1102 7 0.39669737398514116 1102 8 0.3701300032825863 1102 9 0.7844769078623202 1102 10-11 0.88227547669565 1102 12-13 0.9688824404773158 1102 14-15 1.18820095686916 1102 16-17 0.7760353001745699 1102 18-19 0.5354419018791035 1102 20-21 1.0261868515176502 1102 22-23 0.891726321555673 1102 24-25 0.5495500539066533 1102 26-27 1.065549776363305 1102 28-29 0.636017096436106 1102 30-31 0.6445287225053278 1102 32-33 0.9462914450936886 1102 34-35 0.8364394958521046 1102 36-37 0.7239529830639029 1102 38-39 0.9388545491301983 1102 40-41 0.817642710601902 1102 42-43 1.0747275221232897 1102 44-45 0.7330060992184109 1102 46-47 0.5312720829279378 1102 48-49 0.46953970878303863 1102 50-51 0.5309569015649593 1102 52-53 0.4005769001143342 1102 54-55 0.385185908195929 1102 56-57 0.4350462136374489 1102 58-59 0.3502275666284582 1102 60-61 0.3726229199368625 1102 62-63 0.13361453423923209 1102 64-65 0.09424741742481757 1102 66-67 0.06338232399722443 1102 68-69 -0.0047917026494275206 1102 70-71 0.24227765295604797 1102 72-73 -0.019053407610567064 1102 74-75 -0.00549427431603533 1102 76-77 -0.10416168707817874 1102 78-79 -0.06677798378481015 1102 80-81 0.0914780785214866 1102 82-83 0.04465184631515484 1102 84-85 -0.03345139313356427 1102 86-87 0.11807218858117352 1102 88-89 -0.010864475107570826 1102 90-91 0.1514678827412581 1102 92-93 0.23070418058194164 1102 94-95 0.1759482792379643 1102 96-97 -0.11026133910966962 1102 98-99 0.12844837163281753 1102 100-101 0.19342423726331148 1102 102-103 0.13828292303197287 1102 104-105 0.1539609007184204 1102 106-107 0.07242511237036808 1102 108-109 0.11047230121451435 1102 110-111 0.18890024448333165 1102 112-113 0.00624312202091204 1102 114-115 0.09889747659546444 1102 116-117 0.2569995564011087 1102 118-119 0.1610897492540353 1102 120-121 0.20587073252352894 1102 122-123 0.03563946344761604 1102 124-125 0.03373885458470127 1103 1 0.6720971816490504 1103 2 0.9158808064287633 1103 3 0.5902791231932483 1103 4 0.3354827036955541 1103 5 0.5226419117259233 1103 6 0.4440110386829197 1103 7 0.5269956353229333 1103 8 0.747943835904664 1103 9 1.235524682892688 1103 10-11 1.2021621932408095 1103 12-13 1.0740116848921595 1103 14-15 1.3239058950051898 1103 16-17 1.0689657806486679 1103 18-19 1.144998935053497 1103 20-21 1.300223865397193 1103 22-23 1.094118084204581 1103 24-25 0.9593104318763537 1103 26-27 1.070591058372802 1103 28-29 1.172231058320314 1103 30-31 1.203925314574679 1103 32-33 1.3629935368257158 1103 34-35 1.079148648390074 1103 36-37 0.9549747177191428 1103 38-39 0.8552678281765864 1103 40-41 1.0029038464711988 1103 42-43 1.2201800852054916 1103 44-45 0.9864953205518603 1103 46-47 0.6581056006684172 1103 48-49 0.5228133735139195 1103 50-51 0.5426274433401659 1103 52-53 0.5168798005612381 1103 54-55 0.6031399512586404 1103 56-57 0.5480432666656228 1103 58-59 0.5697773612811616 1103 60-61 0.5505345847811078 1103 62-63 0.2519102668177773 1103 64-65 0.28601771224795414 1103 66-67 0.3314152567124289 1103 68-69 0.36006617494044235 1103 70-71 0.49673328975308806 1103 72-73 0.3445945563928454 1103 74-75 0.4335923503994863 1103 76-77 0.47146932681868137 1103 78-79 0.40707293041804604 1103 80-81 0.553833559015267 1103 82-83 0.6282647178552097 1103 84-85 0.604426454549678 1103 86-87 0.519319037718418 1103 88-89 0.539544605674962 1103 90-91 0.47036545273922314 1103 92-93 0.53753321803568 1103 94-95 0.4751865616103714 1103 96-97 0.5020255889624892 1103 98-99 0.47319802269592515 1103 100-101 0.5457792070457295 1103 102-103 0.3865257905886743 1103 104-105 0.46103533055199364 1103 106-107 0.5714284871982613 1103 108-109 0.688394505015701 1103 110-111 0.4613525771058029 1103 112-113 0.520976529707518 1103 114-115 0.5899883924672302 1103 116-117 0.5767199965457372 1103 118-119 0.6296866342493139 1103 120-121 0.702550788880874 1103 122-123 0.6477032136080147 1103 124-125 0.46181657442281576 1104 1 0.759173190548001 1104 2 1.1030572704282733 1104 3 0.5859418954111391 1104 4 0.75335982559168 1104 5 0.4223551094135978 1104 6 0.3923174744574709 1104 7 0.33677480173323104 1104 8 0.6443647407758633 1104 9 0.9610992923540778 1104 10-11 1.057389601605891 1104 12-13 1.0244280873327973 1104 14-15 0.9737151950882996 1104 16-17 0.70159696803805 1104 18-19 0.741000799192328 1104 20-21 1.0184449510056623 1104 22-23 0.921234041793042 1104 24-25 0.8595549072268085 1104 26-27 1.0563055420610183 1104 28-29 1.1404767886213705 1104 30-31 1.1304217450707412 1104 32-33 1.133671957828117 1104 34-35 1.0406225410171217 1104 36-37 0.8391108267131635 1104 38-39 0.9214855693454993 1104 40-41 0.9171464595877161 1104 42-43 1.207551120838808 1104 44-45 0.9364730950870701 1104 46-47 0.7835913987535683 1104 48-49 0.7125553381059504 1104 50-51 0.7524872633762847 1104 52-53 0.5277671114066784 1104 54-55 0.6808399523444351 1104 56-57 0.663157506602353 1104 58-59 0.6292110972032106 1104 60-61 0.6373919345380124 1104 62-63 0.5023810875970014 1104 64-65 0.5161262546174932 1104 66-67 0.46743424403129197 1104 68-69 0.2762053340663897 1104 70-71 0.38887959055377763 1104 72-73 0.36640172812697 1104 74-75 0.2895686478723043 1104 76-77 0.3605383746277653 1104 78-79 0.4049143172718104 1104 80-81 0.5152589004073569 1104 82-83 0.570413980781268 1104 84-85 0.5406717063389053 1104 86-87 0.46934386337686007 1104 88-89 0.4072554675696267 1104 90-91 0.46219908263432075 1104 92-93 0.38006151395257604 1104 94-95 0.3235746987078194 1104 96-97 0.2716207388107925 1104 98-99 0.2575960369099377 1104 100-101 0.40883879395972755 1104 102-103 0.28028294945517374 1104 104-105 0.33105938853421435 1104 106-107 0.2861919105313113 1104 108-109 0.33971163168170904 1104 110-111 0.33433635551672936 1104 112-113 0.284431722275194 1104 114-115 0.35047169703334546 1104 116-117 0.35215484321717483 1104 118-119 0.3272871126849459 1104 120-121 0.25698281896158903 1104 122-123 0.21459369535627104 1104 124-125 0.051996314475839966 1105 1 0.9324675429616853 1105 2 1.1902035103655209 1105 3 0.670682141302624 1105 4 0.8902179555895877 1105 5 0.7331178612282017 1105 6 0.5797156051681291 1105 7 0.6769976857833377 1105 8 0.7359968762885387 1105 9 0.9611389921790838 1105 10-11 0.8971152222664891 1105 12-13 0.9474600977633258 1105 14-15 1.3262389775916112 1105 16-17 0.9114277387630665 1105 18-19 1.021398678224564 1105 20-21 0.957995804727318 1105 22-23 1.0694697030933398 1105 24-25 0.7009253931951349 1105 26-27 1.0725002778272916 1105 28-29 1.2366396435816611 1105 30-31 1.198282410133288 1105 32-33 1.2251797082495344 1105 34-35 1.0568904214028407 1105 36-37 0.6760301544529455 1105 38-39 0.9279884468442106 1105 40-41 1.1154842463797152 1105 42-43 1.1918037005426712 1105 44-45 0.958570317742705 1105 46-47 0.4713550679111087 1105 48-49 0.5567656310334144 1105 50-51 0.7358729789360865 1105 52-53 0.4995339576581159 1105 54-55 0.5822656330717919 1105 56-57 0.5486516244731625 1105 58-59 0.6636494259255166 1105 60-61 0.6027456895228802 1105 62-63 0.5498075372084124 1105 64-65 0.4157706595918995 1105 66-67 0.2951498367007481 1105 68-69 0.14513333815676432 1105 70-71 0.4911539565983496 1105 72-73 0.4007366064913178 1105 74-75 0.5006215269313117 1105 76-77 0.504300050300138 1105 78-79 0.47928600752971207 1105 80-81 0.5788567044346209 1105 82-83 0.5341123223072302 1105 84-85 0.37430368083647636 1105 86-87 0.5181538057271737 1105 88-89 0.5530190530805577 1105 90-91 0.3342074469258236 1105 92-93 0.5425008032259981 1105 94-95 0.4028768533373466 1105 96-97 0.39559699111407554 1105 98-99 0.5237899417496408 1105 100-101 0.5678597898122923 1105 102-103 0.3776927251400828 1105 104-105 0.4988681253024012 1105 106-107 0.5112833632328488 1105 108-109 0.5546829065501839 1105 110-111 0.525867145685428 1105 112-113 0.4923050377331428 1105 114-115 0.5771989671755087 1105 116-117 0.5262297588181148 1105 118-119 0.5890476780547722 1105 120-121 0.4308387161674787 1105 122-123 0.41675104385851114 1105 124-125 0.42908161702820635 1106 1 0.26437358482021267 1106 2 -1.538037423155295 1106 3 -0.21214142853520102 1106 4 0.0740085102935808 1106 5 0.19792560277361915 1106 6 0.17425290415147998 1106 7 0.3299329321549145 1106 8 0.39071352408281257 1106 9 0.7409579307092002 1106 10-11 1.0319802593372494 1106 12-13 0.9893511593044941 1106 14-15 0.9764887047898263 1106 16-17 0.5867644689910847 1106 18-19 0.5442743443147293 1106 20-21 0.9023118678565112 1106 22-23 0.5761842842549179 1106 24-25 0.7160604265014001 1106 26-27 0.6550789764816329 1106 28-29 0.8093955938115727 1106 30-31 0.908700316627467 1106 32-33 1.0903365334611088 1106 34-35 0.897940890674576 1106 36-37 0.5545820204816039 1106 38-39 0.9280287660689481 1106 40-41 0.9814462074613992 1106 42-43 1.0442443735050375 1106 44-45 0.9476303226927065 1106 46-47 0.8112614110741134 1106 48-49 0.6162478159521854 1106 50-51 0.6188870434894369 1106 52-53 0.5360316191203793 1106 54-55 0.514050907955145 1106 56-57 0.6362115181593566 1106 58-59 0.49436552850216486 1106 60-61 0.581700961200216 1106 62-63 0.5231159153026717 1106 64-65 0.47160150282716984 1106 66-67 0.43406972553410483 1106 68-69 0.33585262449922126 1106 70-71 0.3915817036168292 1106 72-73 0.2305615543112971 1106 74-75 0.25110511925436896 1106 76-77 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2216 124-125 -1.2209884323094862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 348585.0 3 42627.0 4 26016.0 5 21497.0 6 20655.0 7 22136.0 8 24080.0 9 24854.0 10 26442.0 11 27090.0 12 27708.0 13 27558.0 14 26671.0 15 22989.0 16 19351.0 17 17325.0 18 15492.0 19 14957.0 20 14877.0 21 15871.0 22 19830.0 23 27652.0 24 38546.0 25 48871.0 26 55934.0 27 62468.0 28 71884.0 29 81970.0 30 94399.0 31 108297.0 32 131092.0 33 162836.0 34 210191.0 35 280285.0 36 382787.0 37 371222.0 38 76863.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93831831787876 18.584278050601228 13.472791543885027 24.00461208763499 2 13.481175513724844 20.03260656870275 41.76902116398595 24.717196753586457 3 18.876466070011784 26.192241577640658 27.787468249785334 27.143824102562224 4 12.190386656315706 16.75637203269874 36.20453755574405 34.84870375524151 5 13.56122917901551 38.94285010223179 33.53285736168739 13.963063357065314 6 32.26731244147999 37.696006697318346 16.511977512342856 13.524703348858813 7 29.090904856441412 33.20367695766322 19.785656085548588 17.91976210034678 8 26.358999912847302 36.420279567217015 18.913165977982967 18.30755454195271 9 26.126704201851748 17.853429160487863 19.34449143549796 36.67537520216243 10-11 24.53547776758038 27.943877492500036 26.775899783516355 20.744744956403224 12-13 25.4758205242676 24.928025645566105 27.123411804708613 22.47274202545768 14-15 22.37652516874535 26.336413756655812 24.79660675054558 26.49045432405326 16-17 22.76826009069258 29.840801685834585 25.128095996142864 22.26284222732997 18-19 22.751089146911692 28.29989313904795 27.015981915122538 21.93303579891782 20-21 23.143819716511132 27.40117466293293 27.14040082213102 22.314604798424913 22-23 23.099870451477184 27.131342642620215 26.77719234138539 22.99159456451721 24-25 23.14690500435641 27.27681993340741 26.78518358863931 22.79109147359687 26-27 23.03373301082259 27.380023957021788 26.87432746709653 22.711915565059087 28-29 22.757321459153964 27.323171775989042 27.169185440384958 22.75032132447204 30-31 22.646931158517397 27.312164697820535 27.115598717466828 22.925305426195244 32-33 22.56518849045819 27.362477326447436 26.9248467877353 23.14748739535907 34-35 23.209489674800263 27.118519528275854 26.753954031166778 22.918036765757112 36-37 23.230070069224716 26.992303174369113 26.547141082868674 23.230485673537494 38-39 23.02189175085782 27.082806047328862 26.968745977798612 22.926556224014707 40-41 22.964043202843733 26.92543487755768 27.367406129550652 22.743115790047938 42-43 22.839697985208833 27.10513679792088 26.93498926071619 23.120175956154096 44-45 22.984903789989545 27.272424962476872 26.653033582844593 23.08963766468899 46-47 22.979702429085183 27.356600983125144 26.161343178771308 23.502353409018365 48-49 23.30031990418502 27.423363146944784 26.050278922799713 23.226038026070476 50-51 23.20202399074793 27.367950797485875 26.219538553518973 23.210486658247227 52-53 23.487718528052575 27.21649347388306 26.13172296471457 23.164065033349797 54-55 23.03966136234548 27.107872414293066 26.392450819857483 23.460015403503963 56-57 23.119000786455015 27.62525501593694 26.061589570243072 23.194154627364977 58-59 23.29631479103762 27.034218990099944 26.21175772026775 23.45770849859468 60-61 23.161996786348716 27.046973673163517 26.47854143789698 23.31248810259079 62-63 23.3628188244791 27.167833574428148 26.380549017938982 23.08879858315377 64-65 23.10859324521394 27.050777337810615 26.37089037913659 23.469739037838856 66-67 22.971612312168165 27.533062125948547 26.067272417823368 23.42805314405992 68-69 23.02095053014164 27.282378148700282 26.431339215356132 23.265332105801946 70-71 22.974166130344827 27.274575286289487 26.329241552266115 23.422017031099575 72-73 23.222921057931796 27.16482308311514 26.068510715517807 23.543745143435256 74-75 23.00608333135303 27.418297454911773 26.125388049802655 23.450231163932543 76-77 23.334029219229322 27.15479183925302 26.353703065931423 23.15747587558624 78-79 23.19958803273664 27.196903862558784 26.340372991277277 23.2631351134273 80-81 22.984392546125402 27.754985333465214 26.22801993884026 23.03260218156912 82-83 23.223692697672995 27.46445390478394 26.04588508480392 23.26596831273914 84-85 23.037927299637172 27.290844004257952 26.0362471235212 23.634981572583673 86-87 23.26556474754416 27.234631693179374 26.344970527240918 23.154833032035548 88-89 23.2056071099383 27.203633974375172 26.218130236257547 23.37262867942898 90-91 23.053266033264027 27.405694040692236 26.401645629992252 23.13939429605148 92-93 23.08283474494068 27.344587292491724 26.429398936045533 23.14317902652206 94-95 22.87690536265801 27.16952583644745 26.382664577193427 23.57090422370111 96-97 23.169762988642034 27.00101047616736 26.271582344497563 23.557644190693043 98-99 22.95565709733481 27.01350465765558 26.17741416910131 23.853424075908297 100-101 22.787472905160207 27.093426980102777 26.564868798728536 23.55423131600848 102-103 23.224424779938214 26.8049888431085 26.593440213361003 23.377146163592286 104-105 22.826809066486806 27.00577596195255 26.662170133501494 23.505244838059152 106-107 22.852186667136515 27.11749489955523 26.57522046520843 23.45509796809982 108-109 22.899413418659094 27.188558821211885 26.087210212759164 23.824817547369857 110-111 22.625474373288775 27.208343319118892 26.5448605807057 23.62132172688663 112-113 22.972455554739405 27.086340583527573 26.49935969044448 23.44184417128854 114-115 22.690059023746763 27.365312090678344 26.31485624282726 23.62977264274763 116-117 22.496820405641955 27.204215461578386 26.68776923515404 23.61119489762562 118-119 22.602280961694685 27.52937689039556 26.15190027800436 23.716441869905395 120-121 22.409226864468422 27.516653967327752 26.334801317098115 23.739317851105707 122-123 22.52043222908361 27.347419108282793 26.333589658916353 23.798559003717248 124-125 22.6421512897977 27.28187659030013 26.335295385551653 23.740676734350515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8058.0 1 5785.5 2 2696.5 3 1548.0 4 1076.0 5 899.0 6 893.0 7 1016.5 8 1223.0 9 1296.5 10 1237.0 11 1207.0 12 1251.0 13 1334.5 14 1429.0 15 1571.0 16 1719.5 17 1882.5 18 2103.5 19 2335.5 20 2764.0 21 3412.5 22 4265.5 23 5418.0 24 6840.0 25 8771.5 26 11531.5 27 14858.5 28 18290.0 29 22853.0 30 28113.5 31 33555.5 32 40947.5 33 47943.0 34 53433.0 35 58579.0 36 65768.5 37 73938.5 38 79364.0 39 82503.5 40 84388.0 41 88548.0 42 93851.5 43 99093.0 44 103147.0 45 106590.0 46 109225.0 47 112053.0 48 122234.0 49 124123.5 50 118891.5 51 115669.0 52 110984.0 53 109388.5 54 107013.5 55 99730.5 56 91106.5 57 83291.5 58 75378.0 59 67030.0 60 57556.0 61 48032.0 62 39317.5 63 31286.0 64 24924.0 65 20354.5 66 15682.5 67 12305.5 68 9388.0 69 6646.0 70 5211.0 71 4318.5 72 3564.0 73 2953.0 74 2321.5 75 1851.0 76 1351.5 77 928.5 78 668.0 79 480.0 80 349.0 81 249.0 82 189.5 83 146.0 84 106.5 85 82.0 86 62.0 87 46.5 88 35.0 89 27.5 90 24.0 91 17.0 92 13.0 93 13.5 94 11.0 95 8.5 96 5.0 97 3.0 98 2.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19728043835965733 2 0.2337351541655987 3 0.2799841697554679 4 0.2672017785120367 5 0.2980123215611383 6 0.21826348060861445 7 0.20979722147335486 8 0.19050743105144963 9 0.1895778025973819 10-11 0.13637317411011315 12-13 0.15964708647802292 14-15 0.17531796613230738 16-17 0.15084881718059626 18-19 0.17413933005661436 20-21 0.17052041928899359 22-23 0.1907896396892916 24-25 0.1814601541323977 26-27 0.23212490487908854 28-29 0.16172214999156695 30-31 0.14324578446697098 32-33 0.1800989124675128 34-35 0.1416355351804608 36-37 0.14234935702911997 38-39 0.1728776914403796 40-41 0.19140385848930064 42-43 0.25891812496596894 44-45 0.15971348851045633 46-47 0.14621727541836602 48-49 0.1491721658616527 50-51 0.1537207050833412 52-53 0.17234647518091234 54-55 0.16095852661858276 56-57 0.1590660686942306 58-59 0.13592496039118765 60-61 0.14852474604542695 62-63 0.1377510162831064 64-65 0.1337834948452102 66-67 0.12217973967747205 68-69 0.1245204113207497 70-71 0.12656227381807703 72-73 0.10431759295288509 74-75 0.10493181175289412 76-77 0.12616386162347656 78-79 0.13114401405598222 80-81 0.14138652755883552 82-83 0.1431627819264292 84-85 0.1307456018613818 86-87 0.1556297635158017 88-89 0.15139663394817188 90-91 0.1405233011372012 92-93 0.13992568284530055 94-95 0.15997909664018997 96-97 0.14817613537515156 98-99 0.14366079716967978 100-101 0.12525083367751721 102-103 0.16089212458614935 104-105 0.12325877270451494 106-107 0.12898594800189644 108-109 0.13907905693177458 110-111 0.11663516996928243 112-113 0.10806930778537269 114-115 0.1051476183583027 116-117 0.11014437129891672 118-119 0.0939754764013817 120-121 0.0867542553742485 122-123 0.11329846783950365 124-125 0.10911513979619887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3011956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.87353300314249 #Duplication Level Percentage of deduplicated Percentage of total 1 78.63861435275086 41.57901371301567 2 9.885219605792987 10.453329701404131 3 4.139555045491315 6.5661870094833015 4 2.229182312009498 4.714589781762226 5 1.3108696793761756 3.4655155627657517 6 0.8088597311305306 2.5660363013305836 7 0.5848404976467584 2.1645808347730107 8 0.4089710464316275 1.7298995296665902 9 0.32016906751637025 1.5235622782120941 >10 1.5224915592827504 14.122662337168004 >50 0.09263523527199158 3.404695845929271 >100 0.05384587452990857 5.254121216945592 >500 0.0034935780106312205 1.316515215032654 >1k 0.0011864981922622996 0.9082888499591231 >5k 6.591656623679443E-5 0.23100182255205354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6628 0.2200563354843165 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.320101621670436E-5 0.0 6 0.0 0.0 0.0 3.320101621670436E-5 0.0 7 0.0 0.0 0.0 3.320101621670436E-5 3.320101621670436E-5 8 0.0 0.0 0.0 3.320101621670436E-5 3.320101621670436E-5 9 0.0 0.0 0.0 3.320101621670436E-5 3.320101621670436E-5 10-11 0.0 0.0 0.0 3.320101621670436E-5 3.320101621670436E-5 12-13 0.0 0.0 0.0 3.320101621670436E-5 3.320101621670436E-5 14-15 0.0 0.0 0.0 3.320101621670436E-5 3.320101621670436E-5 16-17 0.0 0.0 0.0 4.980152432505654E-5 3.320101621670436E-5 18-19 0.0 0.0 0.0 1.1620355675846526E-4 3.320101621670436E-5 20-21 0.0 0.0 0.0 1.9920609730022615E-4 3.320101621670436E-5 22-23 0.0 0.0 0.0 3.486106702753958E-4 3.320101621670436E-5 24-25 0.0 1.660050810835218E-5 0.0 5.47816767575622E-4 3.320101621670436E-5 26-27 0.0 3.320101621670436E-5 0.0 9.628294702844265E-4 3.320101621670436E-5 28-29 0.0 3.320101621670436E-5 0.0 0.001560447762185105 3.320101621670436E-5 30-31 0.0 3.320101621670436E-5 0.0 0.003784915848704297 3.320101621670436E-5 32-33 0.0 3.320101621670436E-5 0.0 0.007038615437941324 3.320101621670436E-5 34-35 0.0 3.320101621670436E-5 0.0 0.011205342973137723 3.320101621670436E-5 36-37 0.0 6.640203243340872E-5 0.0 0.01972140363272239 3.320101621670436E-5 38-39 0.0 6.640203243340872E-5 0.0 0.03330061926535448 3.320101621670436E-5 40-41 0.0 6.640203243340872E-5 0.0 0.05074775328723262 3.320101621670436E-5 42-43 0.0 6.640203243340872E-5 0.0 0.06615302481178344 3.320101621670436E-5 44-45 0.0 6.640203243340872E-5 0.0 0.08411477458502051 6.640203243340872E-5 46-47 0.0 6.640203243340872E-5 0.0 0.10763769457455555 6.640203243340872E-5 48-49 0.0 6.640203243340872E-5 0.0 0.13474632431549466 6.640203243340872E-5 50-51 0.0 6.640203243340872E-5 0.0 0.16308339165645183 6.640203243340872E-5 52-53 0.0 6.640203243340872E-5 0.0 0.1912378534082171 6.640203243340872E-5 54-55 0.0 6.640203243340872E-5 0.0 0.22233060509516075 6.640203243340872E-5 56-57 0.0 6.640203243340872E-5 0.0 0.2587355193767771 6.640203243340872E-5 58-59 0.0 6.640203243340872E-5 0.0 0.3016312323287591 6.640203243340872E-5 60-61 0.0 6.640203243340872E-5 0.0 0.3524619881565335 6.640203243340872E-5 62-63 0.0 6.640203243340872E-5 0.0 0.4041061688816171 6.640203243340872E-5 64-65 0.0 6.640203243340872E-5 0.0 0.45357568304450663 6.640203243340872E-5 66-67 0.0 6.640203243340872E-5 0.0 0.5130553035967325 6.640203243340872E-5 68-69 0.0 6.640203243340872E-5 0.0 0.5822960229166694 6.640203243340872E-5 70-71 0.0 6.640203243340872E-5 0.0 0.6622440699664935 6.640203243340872E-5 72-73 0.0 6.640203243340872E-5 0.0 0.7470062643677398 6.640203243340872E-5 74-75 0.0 6.640203243340872E-5 0.0 0.8315360516554691 6.640203243340872E-5 76-77 0.0 6.640203243340872E-5 0.0 0.9277691971595867 6.640203243340872E-5 78-79 0.0 6.640203243340872E-5 0.0 1.0434083366423679 6.640203243340872E-5 80-81 0.0 6.640203243340872E-5 0.0 1.174635353238892 6.640203243340872E-5 82-83 0.0 6.640203243340872E-5 0.0 1.3131831939111993 6.640203243340872E-5 84-85 0.0 6.640203243340872E-5 0.0 1.4577570190268383 6.640203243340872E-5 86-87 0.0 6.640203243340872E-5 0.0 1.6132041769534482 6.640203243340872E-5 88-89 0.0 6.640203243340872E-5 0.0 1.786596484145187 6.640203243340872E-5 90-91 0.0 6.640203243340872E-5 0.0 1.9851717621372953 6.640203243340872E-5 92-93 0.0 6.640203243340872E-5 0.0 2.2089798124541 6.640203243340872E-5 94-95 0.0 6.640203243340872E-5 0.0 2.4289033438735492 6.640203243340872E-5 96-97 0.0 6.640203243340872E-5 0.0 2.661725470093189 6.640203243340872E-5 98-99 0.0 6.640203243340872E-5 0.0 2.9126089491347154 6.640203243340872E-5 100-101 0.0 6.640203243340872E-5 0.0 3.2076829807606755 6.640203243340872E-5 102-103 0.0 6.640203243340872E-5 0.0 3.5332355452735698 6.640203243340872E-5 104-105 0.0 6.640203243340872E-5 0.0 3.8572110615161708 6.640203243340872E-5 106-107 0.0 6.640203243340872E-5 0.0 4.188391198277797 6.640203243340872E-5 108-109 3.320101621670436E-5 9.960304865011309E-5 0.0 4.5421148250505645 6.640203243340872E-5 110-111 3.320101621670436E-5 9.960304865011309E-5 0.0 4.937804536321248 6.640203243340872E-5 112-113 3.320101621670436E-5 9.960304865011309E-5 0.0 5.376373360035804 6.640203243340872E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13185 0.0 31.987263 1 TACATGG 13760 0.0 30.044 2 ACATGGG 13380 0.0 29.071928 3 CATGGGG 5530 0.0 23.806257 4 CATGGGA 9085 0.0 22.818039 4 GTATCAA 13675 0.0 22.608097 1 GTATAGG 975 0.0 22.605864 1 CGCAGAT 725 0.0 21.336563 8 GTCCAAG 2265 0.0 20.514002 1 ATCAACG 15075 0.0 19.520296 3 TCAACGC 15035 0.0 19.414072 4 AGTACAT 10435 0.0 19.237745 2 ATGGGAG 3900 0.0 19.093107 5 AACGCAG 15120 0.0 18.895222 6 TATCAAC 15935 0.0 18.765858 2 CAACGCA 15500 0.0 18.716665 5 GAGTACT 4015 0.0 18.520634 12-13 ATGGGAT 4035 0.0 17.568497 5 TACGGTT 275 2.8645527E-5 17.31115 7 ACGCAGA 16535 0.0 17.130552 7 >>END_MODULE