FastQCFastQC Report
Thu 26 May 2016
SRR1364605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011956
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84210.2795857575608674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72150.239545332003522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51420.17071962538629382No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31180.1035207685636842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129100.029.8134541
TACATGG138200.027.7470472
GGTATCA126750.027.2167741
ACATGGG134750.026.9795043
GTATCAA165350.026.7726081
CATGGGG57050.023.5715834
GAGTACT105950.022.45310612-13
ATCAACG198550.021.8163973
TCAACGC199950.021.6643644
GTACTTT111750.021.6074714-15
TATCAAC202950.021.3883572
AACGCAG201500.021.219686
CAACGCA201650.021.2095365
AGTACTT115200.021.01206212-13
CATGGGA93000.020.8578994
GTATTAC5750.020.7237421
CGCGGAA6550.019.52555382-83
TATAGGC13650.019.1796883
ACGCAGA226400.018.6757747
ACTTTTT135350.018.65569716-17
Produced by FastQC (version 0.11.5)