##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3011956 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.427346216212985 31.0 30.0 33.0 26.0 34.0 2 29.649463338773874 31.0 30.0 34.0 26.0 34.0 3 29.65213801264029 31.0 30.0 34.0 25.0 34.0 4 33.31904084920231 35.0 33.0 37.0 28.0 37.0 5 32.732162090017255 35.0 33.0 37.0 26.0 37.0 6 32.73475973752605 35.0 33.0 37.0 25.0 37.0 7 32.5248290479675 35.0 33.0 37.0 25.0 37.0 8 32.44732857983317 35.0 33.0 37.0 23.0 37.0 9 33.74496506589074 38.0 33.0 39.0 23.0 39.0 10-11 33.52743117761349 37.0 33.0 39.0 20.5 39.0 12-13 33.39196372722576 37.0 33.0 39.0 19.0 39.0 14-15 34.36397527055509 38.0 33.0 41.0 19.0 41.0 16-17 34.26834140339368 38.0 33.0 41.0 18.5 41.0 18-19 34.28902945461355 38.5 33.0 41.0 18.0 41.0 20-21 34.309006506071135 39.0 33.0 41.0 18.0 41.0 22-23 34.29492861117493 39.0 33.0 41.0 18.5 41.0 24-25 34.27294655034801 39.0 33.0 41.0 19.0 41.0 26-27 34.17130429528187 39.0 33.0 41.0 18.0 41.0 28-29 33.93917490826559 38.0 33.0 41.0 17.0 41.0 30-31 33.86897318553126 38.0 33.0 41.0 17.0 41.0 32-33 33.70698011524737 38.0 33.0 40.0 16.0 41.0 34-35 33.57505952942208 38.0 33.0 40.0 16.0 41.0 36-37 33.403343209528956 38.0 32.0 40.0 15.0 41.0 38-39 33.27329250493699 38.0 32.0 40.0 11.5 41.0 40-41 33.09689251768618 38.0 32.0 40.0 9.0 41.0 42-43 32.93028168406178 38.0 32.0 40.0 8.5 41.0 44-45 32.73629578254131 38.0 31.5 40.0 7.5 41.0 46-47 32.48832967679475 38.0 31.0 40.0 2.0 41.0 48-49 32.242322264999885 38.0 31.0 40.0 2.0 41.0 50-51 32.03930386101258 37.5 30.5 40.0 2.0 41.0 52-53 31.853419671469304 37.0 30.0 40.0 2.0 41.0 54-55 31.624775229120214 37.0 30.0 40.0 2.0 41.0 56-57 31.396705828371992 37.0 30.0 40.0 2.0 41.0 58-59 31.202784502828063 36.0 29.5 40.0 2.0 41.0 60-61 30.969776948932854 36.0 29.0 40.0 2.0 41.0 62-63 30.69097025321751 35.5 29.0 39.0 2.0 41.0 64-65 30.40732550541907 35.0 29.0 39.0 2.0 41.0 66-67 30.129235619643847 35.0 29.0 39.0 2.0 40.5 68-69 29.821735609683543 35.0 28.0 38.0 2.0 40.0 70-71 29.551404303382917 35.0 28.0 37.5 2.0 40.0 72-73 29.386978760645903 35.0 28.0 37.0 2.0 39.5 74-75 29.107251566755956 34.5 28.0 36.5 2.0 39.0 76-77 28.81390747407997 34.0 28.0 36.0 2.0 39.0 78-79 28.518942009777035 34.0 28.0 36.0 2.0 38.0 80-81 28.26754756709593 34.0 27.5 35.0 2.0 37.0 82-83 28.03353302637887 34.0 27.0 35.0 2.0 37.0 84-85 28.032866017963077 34.0 28.0 35.0 2.0 36.0 86-87 27.905268868469527 34.0 27.5 35.0 2.0 36.0 88-89 27.771389754697612 34.0 27.0 35.0 2.0 36.0 90-91 27.635752813122103 34.0 27.0 35.0 2.0 35.5 92-93 27.519315521209474 34.0 27.0 35.0 2.0 35.0 94-95 27.44378354132663 34.0 27.0 35.0 2.0 35.0 96-97 27.36920824872608 34.0 27.0 35.0 2.0 35.0 98-99 27.28164986473906 34.0 26.5 35.0 2.0 35.0 100-101 27.186056502817436 34.0 26.0 35.0 2.0 35.0 102-103 26.00327577826502 32.0 24.5 34.0 2.0 35.0 104-105 26.934385827681414 33.5 25.5 35.0 2.0 35.0 106-107 27.113301787941126 34.0 26.0 35.0 2.0 35.0 108-109 27.126281725231046 34.0 26.0 35.0 2.0 35.0 110-111 27.072128377705383 34.0 26.0 35.0 2.0 35.0 112-113 27.030767879743266 34.0 26.0 35.0 2.0 35.0 114-115 26.940853385640427 34.0 25.0 35.0 2.0 35.0 116-117 26.82485152505548 34.0 25.0 35.0 2.0 35.0 118-119 26.722939843742736 34.0 25.0 35.0 2.0 35.0 120-121 26.590444050311493 34.0 25.0 35.0 2.0 35.0 122-123 26.409096945639313 34.0 25.0 35.0 2.0 35.0 124-125 25.587261234891876 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9857051281146028 1101 2 1.0496213198734416 1101 3 0.8671343104988551 1101 4 0.9237906853023503 1101 5 1.2011433399795237 1101 6 1.2014666121530695 1101 7 1.1011721533699728 1101 8 1.2472731375827593 1101 9 1.3908915878499144 1101 10-11 1.562198147663068 1101 12-13 1.501239509124801 1101 14-15 1.670955837836523 1101 16-17 1.7772429252110769 1101 18-19 1.731585661503594 1101 20-21 1.7852553330622385 1101 22-23 1.6566953468022447 1101 24-25 1.6814595890904016 1101 26-27 1.6656319349557833 1101 28-29 1.8160502786166433 1101 30-31 1.680016312360614 1101 32-33 1.776938884323748 1101 34-35 1.7348192079652875 1101 36-37 1.7589189140592438 1101 38-39 1.8502912795552007 1101 40-41 1.8360330344568183 1101 42-43 1.897159954091201 1101 44-45 1.7111385997660946 1101 46-47 1.7553414314575093 1101 48-49 1.764222243618832 1101 50-51 1.790396193221504 1101 52-53 1.7493849398592367 1101 54-55 1.6901451538146866 1101 56-57 1.8004842866934574 1101 58-59 1.7473201653928108 1101 60-61 1.7368877610711095 1101 62-63 1.7244007676824715 1101 64-65 1.6427685221037969 1101 66-67 1.6247856323320384 1101 68-69 1.6323753272995276 1101 70-71 1.6381819062876453 1101 72-73 1.6595777487920529 1101 74-75 1.6439902506557118 1101 76-77 1.5511603297184315 1101 78-79 1.5227638397403922 1101 80-81 1.574033175986557 1101 82-83 1.5956553497340806 1101 84-85 1.6707284501590607 1101 86-87 1.5705562114476486 1101 88-89 1.5414789394698403 1101 90-91 1.563960533369194 1101 92-93 1.5937251114636517 1101 94-95 1.5598142927298149 1101 96-97 1.5854157505912916 1101 98-99 1.6269467292690045 1101 100-101 1.600167581655441 1101 102-103 1.5524593065490535 1101 104-105 1.5885884575259794 1101 106-107 1.5632374174602717 1101 108-109 1.5395869923061234 1101 110-111 1.5870428198169435 1101 112-113 1.660730079619885 1101 114-115 1.653141362830734 1101 116-117 1.6859154249087567 1101 118-119 1.7051754247468587 1101 120-121 1.7359321492626272 1101 122-123 1.814296743107544 1101 124-125 1.6053645659665285 1102 1 0.40582097455760646 1102 2 -0.500427746696257 1102 3 0.13776519081449834 1102 4 0.36989214626625966 1102 5 0.7735028359341527 1102 6 0.699048733584867 1102 7 0.6941926863502417 1102 8 0.6330106745575108 1102 9 0.9285659625910796 1102 10-11 0.9441090360904312 1102 12-13 1.0521099696901217 1102 14-15 1.1325301433872212 1102 16-17 1.2570098609474698 1102 18-19 1.1813517454707565 1102 20-21 1.1015300207727563 1102 22-23 1.1416325314922346 1102 24-25 1.0730943654004648 1102 26-27 1.1484426830985939 1102 28-29 1.2750066563771725 1102 30-31 1.1728339649817343 1102 32-33 1.1125136479067947 1102 34-35 1.0935102309585858 1102 36-37 1.022032178194337 1102 38-39 0.8279580262512667 1102 40-41 0.9347825167909107 1102 42-43 0.8481567571885762 1102 44-45 0.6512723343342159 1102 46-47 0.784454384216076 1102 48-49 0.6478673371172547 1102 50-51 0.6513901739227705 1102 52-53 0.6375579589567373 1102 54-55 0.7067124538790956 1102 56-57 0.5776907866347933 1102 58-59 0.7975605643898795 1102 60-61 0.6204546219450506 1102 62-63 0.8160247681890631 1102 64-65 0.69284369049576 1102 66-67 0.6683696538793704 1102 68-69 0.4180698484711911 1102 70-71 0.4032628350482703 1102 72-73 0.37618210615984893 1102 74-75 0.4869368729876413 1102 76-77 0.5501778328477336 1102 78-79 0.6255074107765886 1102 80-81 0.5113409451708861 1102 82-83 0.4363817632048175 1102 84-85 0.5885587967640689 1102 86-87 0.5083249266727385 1102 88-89 0.6930623464224546 1102 90-91 0.5470912048702559 1102 92-93 0.7134606531422634 1102 94-95 0.5024003359907603 1102 96-97 0.3837131056584333 1102 98-99 0.4272073784239936 1102 100-101 0.24230171617631768 1102 102-103 0.28190180270492604 1102 104-105 0.28483919663246127 1102 106-107 0.5137835452804325 1102 108-109 0.6044416437625451 1102 110-111 0.5214374799522759 1102 112-113 0.5547096979573389 1102 114-115 0.635598510194388 1102 116-117 0.6658036187850307 1102 118-119 0.536939759333471 1102 120-121 0.5787589703005693 1102 122-123 0.5917651839405664 1102 124-125 0.5021984853404042 1103 1 0.3725078827130055 1103 2 -0.9982372968215429 1103 3 -0.004414686646263988 1103 4 0.2578661828422497 1103 5 0.6975116426054058 1103 6 0.6813793998043849 1103 7 0.6198487093592178 1103 8 0.706098783780071 1103 9 0.7168979005704514 1103 10-11 0.9496490242978268 1103 12-13 0.986541101198199 1103 14-15 1.0267623375685417 1103 16-17 1.0126209944944478 1103 18-19 1.076698254763535 1103 20-21 1.033567180485484 1103 22-23 0.9774670712458473 1103 24-25 0.8503304102863751 1103 26-27 0.9165271051622668 1103 28-29 0.9533649722538229 1103 30-31 0.8489775254527103 1103 32-33 0.8106047830203522 1103 34-35 0.9472034071856967 1103 36-37 1.0101070187618717 1103 38-39 1.0279435413356524 1103 40-41 1.0536881263106537 1103 42-43 0.974123713868444 1103 44-45 1.0317332065623006 1103 46-47 0.9494263305163955 1103 48-49 0.9256380544827039 1103 50-51 0.9676222537598491 1103 52-53 0.9043049371986633 1103 54-55 0.9486543831439 1103 56-57 0.9304959007546749 1103 58-59 0.8936355065092592 1103 60-61 0.8408114930694524 1103 62-63 0.8063152989903735 1103 64-65 0.7680751391762541 1103 66-67 0.837790766982156 1103 68-69 0.7549332403660927 1103 70-71 0.8916614705534762 1103 72-73 0.8199717527121635 1103 74-75 0.7291547241504119 1103 76-77 0.6560102156084824 1103 78-79 0.6510667922392024 1103 80-81 0.7964103576343682 1103 82-83 0.7558442399915606 1103 84-85 0.7941299098274968 1103 86-87 0.7852188244690623 1103 88-89 0.7843258266438511 1103 90-91 0.9198903488825927 1103 92-93 0.8754804368604319 1103 94-95 0.8538556075160137 1103 96-97 0.86870409588348 1103 98-99 0.8844026323311844 1103 100-101 0.9148833109261645 1103 102-103 0.955535054481885 1103 104-105 0.9045609602968909 1103 106-107 0.9673124279266396 1103 108-109 0.9524929601186081 1103 110-111 0.9604683593913315 1103 112-113 0.9924355573497401 1103 114-115 0.838733018380335 1103 116-117 0.8873163064689962 1103 118-119 0.9761520938295334 1103 120-121 0.9703859215328485 1103 122-123 1.0167845890041427 1103 124-125 0.9825117186884569 1104 1 0.2366559554551877 1104 2 -0.889739406788209 1104 3 -0.011335487795456345 1104 4 0.12288379653499959 1104 5 0.4764901341463954 1104 6 0.35278940386373847 1104 7 0.46166483318862817 1104 8 0.519313587015894 1104 9 0.4514051276801254 1104 10-11 0.725589170506808 1104 12-13 0.5949861777743735 1104 14-15 0.6469965540597116 1104 16-17 0.813346843791038 1104 18-19 0.7154296107559546 1104 20-21 0.7774624877359884 1104 22-23 0.6909374458966084 1104 24-25 0.6412198841500469 1104 26-27 0.5557657370299012 1104 28-29 0.800699721375139 1104 30-31 0.7333582479027001 1104 32-33 0.7081935129619552 1104 34-35 0.6762256305550949 1104 36-37 0.6755735087423602 1104 38-39 0.6442142007314331 1104 40-41 0.6583208475285502 1104 42-43 0.5224397170222019 1104 44-45 0.5498010283753203 1104 46-47 0.5993811414366021 1104 48-49 0.5908644313423892 1104 50-51 0.7005866754910599 1104 52-53 0.5296478436894141 1104 54-55 0.751024354888461 1104 56-57 0.7368800762237449 1104 58-59 0.5806703885357081 1104 60-61 0.6568074081937958 1104 62-63 0.5825713258678036 1104 64-65 0.5687262448109784 1104 66-67 0.6305796203996223 1104 68-69 0.6604380122452937 1104 70-71 0.7649511765106212 1104 72-73 0.7260376139982121 1104 74-75 0.7011717115108027 1104 76-77 0.7127691094613375 1104 78-79 0.6682562629216839 1104 80-81 0.6086854718381112 1104 82-83 0.6499912850381513 1104 84-85 0.6682723459594015 1104 86-87 0.6290877916626201 1104 88-89 0.6020353932039519 1104 90-91 0.6049787049868307 1104 92-93 0.622038323362073 1104 94-95 0.5523275556172855 1104 96-97 0.6481716476573318 1104 98-99 0.6591189492987901 1104 100-101 0.677957046512951 1104 102-103 0.6567586122744764 1104 104-105 0.5981274240087586 1104 106-107 0.6040322769668265 1104 108-109 0.6320937170511733 1104 110-111 0.5168191514217497 1104 112-113 0.5170099113635374 1104 114-115 0.668006565229252 1104 116-117 0.7339368119199747 1104 118-119 0.6637391598376858 1104 120-121 0.6156570264856178 1104 122-123 0.7070111373051695 1104 124-125 0.6181921569839552 1105 1 0.7033210279738427 1105 2 0.4871049039775883 1105 3 0.7554092982788063 1105 4 0.6386588913021782 1105 5 0.7918287062563252 1105 6 0.7335807081617887 1105 7 0.8625628063002182 1105 8 0.7881191171461168 1105 9 0.9309321587370221 1105 10-11 1.0961582545727566 1105 12-13 1.1082845574121762 1105 14-15 1.154246919251932 1105 16-17 1.2597962807090326 1105 18-19 1.1888952863795765 1105 20-21 1.2708867195120632 1105 22-23 1.145270367890518 1105 24-25 1.1292312755993734 1105 26-27 1.0718580380151366 1105 28-29 1.242371053540623 1105 30-31 1.1711611783406752 1105 32-33 1.1745345471996416 1105 34-35 1.1930096779097852 1105 36-37 1.2011536986035338 1105 38-39 1.2513789851032158 1105 40-41 1.0597125987543095 1105 42-43 1.1273318941439854 1105 44-45 1.2003797871962902 1105 46-47 1.222184956349743 1105 48-49 1.331968323753273 1105 50-51 1.2739869202635674 1105 52-53 1.232865662226498 1105 54-55 1.1829938807488354 1105 56-57 1.2244314805727363 1105 58-59 1.040370357569845 1105 60-61 1.1077136813385664 1105 62-63 1.1364425804432052 1105 64-65 1.2085702171126869 1105 66-67 1.1277370206039556 1105 68-69 1.0578152571549921 1105 70-71 1.033236315795147 1105 72-73 0.9676289602488239 1105 74-75 1.0933078178369051 1105 76-77 1.1102333246853533 1105 78-79 1.0721722536603622 1105 80-81 0.8611150088033561 1105 82-83 0.9735186615758522 1105 84-85 0.7570632203914442 1105 86-87 0.8590164141470105 1105 88-89 0.8760425698244099 1105 90-91 0.8677144550116189 1105 92-93 0.7533451252715793 1105 94-95 0.7832381486569666 1105 96-97 0.910594253771734 1105 98-99 0.9927599255008666 1105 100-101 1.012289999897412 1105 102-103 0.5830351139849839 1105 104-105 0.5379237934505312 1105 106-107 0.9161744130500722 1105 108-109 0.9545656552760313 1105 110-111 0.860347253635318 1105 112-113 0.691673861577371 1105 114-115 0.9363224718781602 1105 116-117 0.6084469345444248 1105 118-119 0.4230252258823164 1105 120-121 0.3511215818355744 1105 122-123 0.45381174687290127 1105 124-125 0.38245390365439036 1106 1 0.4349957246590783 1106 2 0.49389665014191664 1106 3 0.6008101347120203 1106 4 0.38219874578881985 1106 5 0.5631916408929101 1106 6 0.5658397475571704 1106 7 0.5641684876422204 1106 8 0.668164156626041 1106 9 0.7347342253061484 1106 10-11 0.8715361303879945 1106 12-13 0.7847045716755474 1106 14-15 0.8732412878640048 1106 16-17 1.0161303832405508 1106 18-19 0.9497323153013113 1106 20-21 1.0322132773994497 1106 22-23 1.0435013528242507 1106 24-25 1.0478488955637104 1106 26-27 0.9701575837927408 1106 28-29 1.1357462734631412 1106 30-31 1.0483275704688353 1106 32-33 0.8578895362246826 1106 34-35 0.9038855345173644 1106 36-37 1.0325518226607997 1106 38-39 1.0692055582238353 1106 40-41 1.1003226622239382 1106 42-43 1.0147627980941962 1106 44-45 1.0377017951863152 1106 46-47 0.8964063015380148 1106 48-49 0.9162788647832372 1106 50-51 1.0152444932287636 1106 52-53 1.0216154906541242 1106 54-55 1.0380038599839772 1106 56-57 0.940501138934966 1106 58-59 0.9560526426237708 1106 60-61 1.0912542886323564 1106 62-63 1.0400163166999938 1106 64-65 0.9772122624452102 1106 66-67 0.9099504125527247 1106 68-69 0.966069542332594 1106 70-71 0.9674311184018975 1106 72-73 1.0159479187342342 1106 74-75 1.052118165511672 1106 76-77 1.0767912520374665 1106 78-79 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86-87 -2.460026522602625 2216 88-89 -2.3184726387731196 2216 90-91 -2.2513034889118835 2216 92-93 -2.3725690336272542 2216 94-95 -2.2572237095133723 2216 96-97 -2.2963032571279136 2216 98-99 -2.2375277002732155 2216 100-101 -2.436474775953961 2216 102-103 -2.2618082643045803 2216 104-105 -2.3295814828127632 2216 106-107 -2.4134154963277936 2216 108-109 -2.4566449231690477 2216 110-111 -2.3959637589444043 2216 112-113 -2.5848974003752154 2216 114-115 -2.571041664342001 2216 116-117 -2.592743209951138 2216 118-119 -2.7808795097954047 2216 120-121 -2.766832037958739 2216 122-123 -2.711337122433129 2216 124-125 -2.8366808585897836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124436.0 3 38780.0 4 18155.0 5 13922.0 6 14954.0 7 17549.0 8 19432.0 9 20706.0 10 21753.0 11 23587.0 12 25802.0 13 27998.0 14 30548.0 15 25158.0 16 17689.0 17 15350.0 18 14195.0 19 13292.0 20 13101.0 21 14142.0 22 16539.0 23 21556.0 24 29586.0 25 38763.0 26 46766.0 27 53478.0 28 62287.0 29 71767.0 30 84061.0 31 99032.0 32 120741.0 33 149184.0 34 192763.0 35 265009.0 36 420027.0 37 617243.0 38 212132.0 39 473.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37560603211663 19.0945712412056 13.602991740621908 24.92683098605586 2 15.609528217474555 20.54368639136024 38.24879647993015 25.59798891123506 3 19.36831082525774 24.65696710044901 29.142756401487937 26.831965672805314 4 13.102750774138752 16.82027695672991 35.37327665723467 34.70369561189667 5 13.511354858335764 38.55856298656918 33.690629510343946 14.239452644751113 6 33.05547338284944 36.87715163835775 16.1525975373421 13.9147774414507 7 28.931882757837602 32.56464076144589 20.22577370039516 18.277702780321345 8 26.83697902625404 35.78886942571538 18.51886282535336 18.855288722677223 9 26.49586809786416 16.107446056183246 19.290986178297636 38.10569966765496 10-11 25.356179635492772 26.772659449696175 26.60099160397233 21.270169310838728 12-13 26.248567231539365 23.738366849821958 27.476547755185525 22.53651816345315 14-15 22.999245947590868 25.103686356698336 25.327876663455882 26.569191032254913 16-17 22.826550022746027 28.595653988627152 25.949640506435074 22.628155482191744 18-19 22.713882131548623 26.820406440875033 28.382350841696958 22.083360585879387 20-21 23.021247700573113 26.12198750174325 28.54950159714705 22.30726320053659 22-23 22.85853558272746 25.877894444635373 28.430436452258316 22.833133520378844 24-25 22.89840887449883 26.15225786830883 28.416849382925914 22.532483874266422 26-27 22.787866091005313 26.333253208214195 28.34232970202752 22.53655099875297 28-29 22.492982730041888 26.15672844078753 28.696099892706357 22.654188936464227 30-31 22.44516892762858 26.17802620257836 28.66604436884941 22.710760500943657 32-33 22.366081826492934 26.321111437046053 28.401146339199997 22.91166039726102 34-35 22.94507671179073 26.05561403546633 28.310046413368323 22.689262839374617 36-37 23.01199587111751 25.89094185732646 28.1188152670377 22.97824700451833 38-39 22.851811248238686 26.064607185496737 28.37738997515236 22.70619159111222 40-41 22.72905426391592 25.919074833608928 28.701818469761587 22.650052432713565 42-43 22.81268027793618 26.10514992068808 28.104540659081607 22.977629142294127 44-45 22.816406152082923 26.23931528868869 27.90602317995734 23.038255379271043 46-47 22.874528337906774 26.39984661125415 27.396292441288132 23.32933260955094 48-49 23.245095414052983 26.54840225680925 27.053971486592665 23.152530842545108 50-51 23.10677725956379 26.588245645051916 27.03724085496178 23.26773624042252 52-53 23.386010645247133 26.451594803307955 26.996058539396927 23.166336012047992 54-55 22.9363078345102 26.391404788117757 27.23373117004365 23.438556207328393 56-57 23.080877887826034 26.815483591646633 26.881255787438608 23.222382733088725 58-59 23.137679679809292 26.31023703873398 27.108356532551113 23.44372674890561 60-61 23.07550309499873 26.335627080873692 27.371448985310543 23.217420838817034 62-63 23.2702270551097 26.38924672206367 27.315837283147566 23.024688939679066 64-65 23.003100163931027 26.229976551638273 27.38434977485423 23.382573509576478 66-67 22.882870798909412 26.776818784869366 27.100063878755197 23.24024653746602 68-69 22.886861289028268 26.50732394501808 27.3980966723889 23.207718093564754 70-71 22.808185112929937 26.456179970756544 27.222542427578624 23.513092488734895 72-73 23.038415675642206 26.444827258198583 27.083027256638108 23.433729809521108 74-75 22.87525266745112 26.73010799956439 27.023331730782 23.37130760220249 76-77 23.15060918287173 26.415011963201845 27.33963059412283 23.09474825980359 78-79 22.9757908162376 26.448458142612946 27.32176701659116 23.253984024558292 80-81 22.78450753092302 27.042728629459994 27.027157140888768 23.14560669872822 82-83 23.081721906304963 26.726666506194903 26.865463515775893 23.32614807172424 84-85 22.850498380785364 26.586179603008546 26.817657241777265 23.745664774428825 86-87 23.09730217696394 26.424983573748147 27.183330251163117 23.2943839981248 88-89 22.986109358835254 26.523843641806188 26.989969308980612 23.500077690377946 90-91 22.801445306637945 26.73862101571205 27.253020960465555 23.206912717184448 92-93 22.830414743024544 26.59985958360179 27.30459783186323 23.265127841510427 94-95 22.677998505217296 26.408464148954998 27.182318002237192 23.731219343590514 96-97 22.920509213313178 26.248720442342627 27.09619185711587 23.73457848722833 98-99 22.728113322498658 26.276629475735696 26.99948704072299 23.99577016104265 100-101 22.574390493315946 26.276434754431026 27.37904489592104 23.77012985633199 102-103 23.064964316348178 25.992582554810355 27.310049773344776 23.632403355496688 104-105 22.691796586759896 26.085630642632385 27.416895546818132 23.805677223789594 106-107 22.688075238894147 26.23930203614294 27.337000740243568 23.73562198471934 108-109 22.73591589980331 26.202356303596876 26.91941061863926 24.142317177960553 110-111 22.4781519407106 26.213321788164297 27.295830790673648 24.012695480451455 112-113 22.78433857486948 26.060225344939187 27.236985376036127 23.918450704155205 114-115 22.443705349620718 26.31892873120918 27.076579317168235 24.160786602001863 116-117 22.2706724753848 26.142252946179163 27.379839932109512 24.207234646326526 118-119 22.295304526941624 26.48410422520528 26.91632294686977 24.304268300983335 120-121 22.029441853932305 26.485266621309183 27.090536656051157 24.394754868707356 122-123 22.093984353925006 26.377721981282814 27.00736652071964 24.520927144072544 124-125 22.110842067832774 26.336736160844275 27.030283471568012 24.52213829975494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 388.0 1 278.5 2 366.5 3 616.0 4 487.0 5 444.0 6 1043.5 7 2274.5 8 3467.5 9 3919.0 10 3127.5 11 2047.5 12 1804.5 13 1875.0 14 2050.5 15 2278.5 16 2507.5 17 2781.0 18 3035.0 19 3276.5 20 3643.0 21 4127.0 22 4933.5 23 5983.0 24 7097.0 25 8803.5 26 11349.5 27 14623.5 28 17873.5 29 22115.0 30 27365.0 31 32651.5 32 39804.0 33 46419.0 34 51738.5 35 56761.0 36 64189.0 37 72733.0 38 78419.0 39 81718.0 40 83419.5 41 87880.5 42 92986.0 43 97999.5 44 102228.5 45 106067.5 46 108760.0 47 111514.0 48 125566.5 49 127999.5 50 120255.0 51 117333.5 52 112320.0 53 110907.5 54 109823.5 55 102900.0 56 93139.0 57 84240.5 58 76472.0 59 68278.5 60 57951.5 61 48053.0 62 39381.0 63 31027.5 64 24536.5 65 19965.0 66 14849.5 67 11594.5 68 8570.0 69 5706.0 70 4415.5 71 3546.0 72 2936.5 73 2504.0 74 1984.0 75 1535.0 76 1070.5 77 661.5 78 452.0 79 291.0 80 192.5 81 125.5 82 86.0 83 64.0 84 49.0 85 34.5 86 21.5 87 14.0 88 9.5 89 5.5 90 4.0 91 4.0 92 2.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059828231222501256 2 0.07446987937406788 3 0.0 4 0.007370625600108368 5 0.027291235330130986 6 2.3240711351693051E-4 7 6.640203243340872E-5 8 0.0 9 8.632264216343133E-4 10-11 0.05918081140627552 12-13 0.010093108929878126 14-15 0.007603032713625299 16-17 0.05114616548183307 18-19 1.660050810835218E-4 20-21 0.011155541448812666 22-23 0.01293179581640635 24-25 0.0 26-27 0.0 28-29 0.050664750746690856 30-31 1.660050810835218E-5 32-33 0.003569109243295719 34-35 1.660050810835218E-5 36-37 9.960304865011309E-5 38-39 0.0 40-41 1.660050810835218E-5 42-43 0.003768315340595945 44-45 1.660050810835218E-5 46-47 3.320101621670436E-5 48-49 1.4940457297516962E-4 50-51 2.8220863784198707E-4 52-53 4.9801524325056544E-5 54-55 0.0 56-57 0.0014940457297516962 58-59 3.320101621670436E-5 60-61 0.0 62-63 0.0 64-65 6.142188000090307E-4 66-67 0.0 68-69 6.806208324424395E-4 70-71 0.0 72-73 0.0015770482702934573 74-75 0.0030544934919368013 76-77 3.8181168649210017E-4 78-79 6.806208324424395E-4 80-81 0.001361241664884879 82-83 1.1620355675846526E-4 84-85 6.640203243340872E-5 86-87 2.9880914595033925E-4 88-89 0.0 90-91 0.0 92-93 0.006623602735232521 94-95 0.004880549383855541 96-97 0.005743775805489854 98-99 6.972213405507916E-4 100-101 3.9841219460045235E-4 102-103 8.30025405417609E-5 104-105 2.8220863784198707E-4 106-107 0.0036355112757291276 108-109 0.003984121946004524 110-111 5.976182919006785E-4 112-113 0.0028054858703115185 114-115 4.482137189255089E-4 116-117 0.001543847254076753 118-119 0.0018260558919187399 120-121 0.010790330270428918 122-123 6.142188000090307E-4 124-125 0.0016268497946185137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3011956.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.96344571049046 #Duplication Level Percentage of deduplicated Percentage of total 1 71.78937034484925 30.12529345056375 2 12.146760452512664 10.194398456146955 3 5.394443392043943 6.7910829726105 4 2.9801492252602797 5.002293208934798 5 1.8453315782374795 3.871823575061107 6 1.2108221331272724 3.0486161309127926 7 0.8245324959935928 2.422015724251363 8 0.6399920043968228 2.148501578532324 9 0.5020599270199618 1.8961348041823471 >10 2.3958781275965264 17.807127239810235 >50 0.16151858577360256 4.697842057003194 >100 0.09963031472993089 7.804421961141717 >500 0.006873798213939149 1.9977725118186316 >1k 0.0023978365862189917 1.4957719210914249 >5k 2.397836586218992E-4 0.6969044079387754 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8421 0.2795857575608674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7215 0.239545332003522 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5142 0.17071962538629382 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3118 0.1035207685636842 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.320101621670436E-5 0.0 6 0.0 0.0 0.0 3.320101621670436E-5 0.0 7 0.0 0.0 0.0 3.320101621670436E-5 0.0 8 0.0 0.0 0.0 3.320101621670436E-5 0.0 9 0.0 0.0 0.0 3.320101621670436E-5 0.0 10-11 0.0 0.0 0.0 6.640203243340872E-5 0.0 12-13 0.0 0.0 0.0 6.640203243340872E-5 0.0 14-15 0.0 0.0 0.0 6.640203243340872E-5 0.0 16-17 0.0 0.0 0.0 8.30025405417609E-5 0.0 18-19 0.0 0.0 0.0 1.1620355675846526E-4 0.0 20-21 0.0 0.0 0.0 1.9920609730022615E-4 3.320101621670436E-5 22-23 0.0 0.0 0.0 3.486106702753958E-4 3.320101621670436E-5 24-25 0.0 0.0 0.0 5.810177837923263E-4 3.320101621670436E-5 26-27 0.0 0.0 0.0 0.001012630994609483 3.320101621670436E-5 28-29 0.0 0.0 0.0 0.0016102492865101613 3.320101621670436E-5 30-31 0.0 0.0 0.0 0.003934320421679466 3.320101621670436E-5 32-33 0.0 0.0 0.0 0.0072544220433499035 3.320101621670436E-5 34-35 0.0 0.0 0.0 0.01150415211908806 3.320101621670436E-5 36-37 0.0 0.0 0.0 0.02023601938408131 3.320101621670436E-5 38-39 0.0 0.0 0.0 0.034562257881589245 3.320101621670436E-5 40-41 0.0 0.0 0.0 0.05292241984942675 3.320101621670436E-5 42-43 0.0 0.0 0.0 0.06922411881182859 3.320101621670436E-5 44-45 0.0 0.0 0.0 0.08776688636885799 3.320101621670436E-5 46-47 0.0 0.0 0.0 0.11165501753677677 3.320101621670436E-5 48-49 0.0 0.0 0.0 0.13909565743988292 3.320101621670436E-5 50-51 0.0 0.0 0.0 0.1678809384997656 3.320101621670436E-5 52-53 0.0 0.0 0.0 0.1964836139704564 3.320101621670436E-5 54-55 0.0 0.0 0.0 0.22827358699795083 3.320101621670436E-5 56-57 0.0 0.0 0.0 0.26559152922552653 3.320101621670436E-5 58-59 0.0 0.0 0.0 0.30963267723698484 3.320101621670436E-5 60-61 0.0 0.0 0.0 0.36160886812423554 3.320101621670436E-5 62-63 0.0 0.0 0.0 0.4134190539304027 3.320101621670436E-5 64-65 0.0 0.0 0.0 0.46385139756357663 3.320101621670436E-5 66-67 0.0 0.0 0.0 0.5233974201482359 3.320101621670436E-5 68-69 0.0 0.0 0.0 0.5937337730033241 3.320101621670436E-5 70-71 0.0 0.0 0.0 0.675325270355875 3.320101621670436E-5 72-73 0.0 0.0 0.0 0.761299301849031 3.320101621670436E-5 74-75 0.0 0.0 0.0 0.8475887429962456 3.320101621670436E-5 76-77 0.0 0.0 0.0 0.9467435779274331 3.320101621670436E-5 78-79 0.0 0.0 0.0 1.0640759692372663 3.320101621670436E-5 80-81 0.0 0.0 0.0 1.197544054428418 3.320101621670436E-5 82-83 0.0 0.0 0.0 1.3375859408304769 3.320101621670436E-5 84-85 0.0 0.0 0.0 1.4834214045623508 3.320101621670436E-5 86-87 0.0 0.0 0.0 1.6410432290511547 3.320101621670436E-5 88-89 0.0 0.0 0.0 1.815464767745611 3.320101621670436E-5 90-91 0.0 0.0 0.0 2.016447119413431 3.320101621670436E-5 92-93 0.0 0.0 0.0 2.2416828134275533 3.320101621670436E-5 94-95 0.0 0.0 0.0 2.464743840879482 3.320101621670436E-5 96-97 0.0 0.0 0.0 2.7001390458559156 3.320101621670436E-5 98-99 0.0 0.0 0.0 2.9560026773299475 3.320101621670436E-5 100-101 0.0 0.0 0.0 3.2570860928911314 3.320101621670436E-5 102-103 0.0 0.0 0.0 3.58622436715543 3.320101621670436E-5 104-105 0.0 0.0 0.0 3.914864626176478 3.320101621670436E-5 106-107 0.0 0.0 0.0 4.251456528581427 3.320101621670436E-5 108-109 0.0 0.0 0.0 4.611421946402935 3.320101621670436E-5 110-111 0.0 0.0 0.0 5.016856155933221 3.320101621670436E-5 112-113 0.0 0.0 0.0 5.46566749315063 3.320101621670436E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12910 0.0 29.813454 1 TACATGG 13820 0.0 27.747047 2 GGTATCA 12675 0.0 27.216774 1 ACATGGG 13475 0.0 26.979504 3 GTATCAA 16535 0.0 26.772608 1 CATGGGG 5705 0.0 23.571583 4 GAGTACT 10595 0.0 22.453106 12-13 ATCAACG 19855 0.0 21.816397 3 TCAACGC 19995 0.0 21.664364 4 GTACTTT 11175 0.0 21.60747 14-15 TATCAAC 20295 0.0 21.388357 2 AACGCAG 20150 0.0 21.21968 6 CAACGCA 20165 0.0 21.209536 5 AGTACTT 11520 0.0 21.012062 12-13 CATGGGA 9300 0.0 20.857899 4 GTATTAC 575 0.0 20.723742 1 CGCGGAA 655 0.0 19.525553 82-83 TATAGGC 1365 0.0 19.179688 3 ACGCAGA 22640 0.0 18.675774 7 ACTTTTT 13535 0.0 18.655697 16-17 >>END_MODULE