FastQCFastQC Report
Thu 26 May 2016
SRR1364604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470623
Sequences flagged as poor quality0
Sequence length125
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34150.1382242454635936No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88050.039.508041
GTACATG137650.037.2588041
TACATGG138950.035.537692
CGCATAG2850.035.4912538
TCAACGC102450.033.428944
ATCAACG103000.033.4259533
CAACGCA103550.033.1326945
ACATGGG142500.033.0639573
TATCAAC109850.031.8838272
AACGCAG104400.031.8690816
GGTATCA55450.031.0454731
AACGCAT3950.027.1227466
ACGCAGA122650.026.7355677
CATGGGG57500.026.310714
CGCAGAG118200.025.5215918
CATGGGA88950.025.311194
ACGCATA4250.023.8048447
GTCCTAC11550.023.723451
GTACTAG5800.022.5941851
CGCAGAT9000.022.4777938
Produced by FastQC (version 0.11.5)