FastQCFastQC Report
Thu 26 May 2016
SRR1364604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470623
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96640.39115640063255297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82850.33534051937507264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52890.21407555907963297No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.102322369701893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81000.046.769631
GTATCAA131050.044.134081
ATCAACG161100.035.5655863
TCAACGC162200.035.3639344
TATCAAC163000.035.2096142
CAACGCA164150.034.5061345
AACGCAG168100.033.7218556
GTACATG138650.033.0368271
TACATGG141600.031.1969782
ACATGGG145250.029.1650733
CGCAGAG197350.028.3315738
ACGCAGA198150.028.307347
GCAGAGT208100.026.096529
GAGTACT115350.024.4513612-13
AGTACTT118400.024.04788212-13
CATGGGG58000.023.8009554
GTACTTT120400.023.67315314-15
CATGGGA93350.022.75564
AGTACAT107350.021.9061932
CAGAGTA207600.021.52659810-11
Produced by FastQC (version 0.11.5)