##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2470623 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.625997977028465 31.0 30.0 33.0 26.0 34.0 2 29.796485744688688 31.0 30.0 34.0 26.0 34.0 3 29.80599630133776 31.0 30.0 34.0 26.0 34.0 4 33.462580086075455 35.0 33.0 37.0 28.0 37.0 5 32.885598490745046 35.0 33.0 37.0 26.0 37.0 6 32.87617414716855 35.0 33.0 37.0 26.0 37.0 7 32.67546080482534 35.0 33.0 37.0 25.0 37.0 8 32.603572054497995 35.0 33.0 37.0 25.0 37.0 9 33.89487064598686 38.0 33.0 39.0 24.0 39.0 10-11 33.687580824755536 38.0 33.0 39.0 22.5 39.0 12-13 33.555561289601854 37.0 33.0 39.0 21.5 39.0 14-15 34.52364322682983 38.0 33.0 41.0 22.0 41.0 16-17 34.445696085562226 38.0 33.0 41.0 20.5 41.0 18-19 34.50264649847427 38.5 33.5 41.0 20.0 41.0 20-21 34.535054923393815 39.0 33.5 41.0 20.0 41.0 22-23 34.529120185475485 39.0 34.0 41.0 21.5 41.0 24-25 34.517364850889834 39.0 33.5 41.0 22.0 41.0 26-27 34.42234428320306 39.0 33.0 41.0 21.5 41.0 28-29 34.18286622443003 39.0 33.0 41.0 18.5 41.0 30-31 34.10281596989909 38.0 33.0 41.0 19.0 41.0 32-33 33.91360094194865 38.0 33.0 41.0 18.0 41.0 34-35 33.771644641857534 38.0 33.0 41.0 16.5 41.0 36-37 33.5929463944924 38.0 33.0 40.0 16.0 41.0 38-39 33.45310757651006 38.0 33.0 40.0 15.0 41.0 40-41 33.26682075735553 38.0 32.0 40.0 11.0 41.0 42-43 33.07653008977897 38.0 32.0 40.0 9.0 41.0 44-45 32.843267062599196 38.0 32.0 40.0 7.5 41.0 46-47 32.54326560547683 38.0 31.0 40.0 2.0 41.0 48-49 32.25551267838112 38.0 31.0 40.0 2.0 41.0 50-51 32.0453088148212 38.0 30.0 40.0 2.0 41.0 52-53 31.88064123907209 37.5 30.0 40.0 2.0 41.0 54-55 31.666044758751134 37.0 30.0 40.0 2.0 41.0 56-57 31.449218678851445 37.0 30.0 40.0 2.0 41.0 58-59 31.266519821113945 37.0 29.5 40.0 2.0 41.0 60-61 31.053607329001633 36.0 29.0 40.0 2.0 41.0 62-63 30.79016001227221 36.0 29.0 39.5 2.0 41.0 64-65 30.519044386780177 35.5 29.0 39.0 2.0 41.0 66-67 30.242050486861007 35.0 28.5 39.0 2.0 41.0 68-69 29.932690459046157 35.0 28.0 39.0 2.0 40.0 70-71 29.670570945061225 35.0 28.0 38.0 2.0 40.0 72-73 29.510348199624143 35.0 28.0 37.0 2.0 40.0 74-75 29.22195474582727 35.0 28.0 37.0 2.0 39.0 76-77 28.92571104535172 34.0 28.0 36.5 2.0 39.0 78-79 28.61682579657034 34.0 28.0 36.0 2.0 39.0 80-81 28.340218236452912 34.0 27.5 36.0 2.0 37.0 82-83 28.091777863316256 34.0 27.0 35.0 2.0 37.0 84-85 28.066158211916587 34.0 28.0 35.0 2.0 36.5 86-87 27.92306454687745 34.0 27.5 35.0 2.0 36.0 88-89 27.77950925738164 34.0 27.0 35.0 2.0 36.0 90-91 27.62685970299799 34.0 27.0 35.0 2.0 36.0 92-93 27.498082872214823 34.0 27.0 35.0 2.0 35.0 94-95 27.41346959855874 34.0 27.0 35.0 2.0 35.0 96-97 27.32972695550879 34.0 27.0 35.0 2.0 35.0 98-99 27.241999892334846 34.0 26.0 35.0 2.0 35.0 100-101 27.14978064237239 34.0 26.0 35.0 2.0 35.0 102-103 25.98342017377803 32.0 24.5 34.0 2.0 35.0 104-105 26.898004268558985 33.5 25.0 35.0 2.0 35.0 106-107 27.064478473648144 34.0 26.0 35.0 2.0 35.0 108-109 27.077709346994666 34.0 26.0 35.0 2.0 35.0 110-111 27.025364452609725 34.0 26.0 35.0 2.0 35.0 112-113 26.97736380661882 34.0 26.0 35.0 2.0 35.0 114-115 26.898955040894542 34.0 25.0 35.0 2.0 35.0 116-117 26.780134200968746 34.0 25.0 35.0 2.0 35.0 118-119 26.67751129978147 34.0 25.0 35.0 2.0 35.0 120-121 26.544708763740967 34.0 25.0 35.0 2.0 35.0 122-123 26.361535126969997 34.0 25.0 35.0 2.0 35.0 124-125 25.56489517016558 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7577306154255083 1101 2 0.7670204405340222 1101 3 0.7310589423722327 1101 4 0.6451711688028325 1101 5 0.988056028209698 1101 6 0.8991357611193109 1101 7 0.947896167476344 1101 8 0.9920564618100585 1101 9 0.9851632110283006 1101 10-11 1.046945459981167 1101 12-13 1.1501298785219305 1101 14-15 1.240037283867352 1101 16-17 1.322707054998986 1101 18-19 1.2336500961145518 1101 20-21 1.3309799753412577 1101 22-23 1.2020558827877537 1101 24-25 1.0801656085640445 1101 26-27 1.2192422855296101 1101 28-29 1.3463535345918203 1101 30-31 1.3495173682844026 1101 32-33 1.2942501884347593 1101 34-35 1.370437385663898 1101 36-37 1.2236514009843802 1101 38-39 1.2879805035825456 1101 40-41 1.376100316119576 1101 42-43 1.2661611086463225 1101 44-45 1.2219052211568524 1101 46-47 1.4683341349959065 1101 48-49 1.5011584188519578 1101 50-51 1.3224342352198448 1101 52-53 1.2867018181395764 1101 54-55 1.4068681248707406 1101 56-57 1.4215724605696884 1101 58-59 1.4622045662816205 1101 60-61 1.339471355237091 1101 62-63 1.301799037934824 1101 64-65 1.3185552200527333 1101 66-67 1.293418893443686 1101 68-69 1.280937243257629 1101 70-71 1.3648095580086341 1101 72-73 1.296225652083919 1101 74-75 1.253813275177766 1101 76-77 1.2618894276465191 1101 78-79 1.2699539503865083 1101 80-81 1.1957109209514627 1101 82-83 1.2172822911988739 1101 84-85 1.1953504781564455 1101 86-87 1.212636823948685 1101 88-89 1.1896239550896865 1101 90-91 1.2802773303900459 1101 92-93 1.174345176278809 1101 94-95 1.1344689575422926 1101 96-97 1.1167492479342442 1101 98-99 1.1317376544075728 1101 100-101 1.217205590548648 1101 102-103 1.1811840995089433 1101 104-105 1.150712018155268 1101 106-107 1.106543906829561 1101 108-109 1.0624837706128183 1101 110-111 1.1124260320378632 1101 112-113 1.122119015003367 1101 114-115 1.0830010958860008 1101 116-117 1.2068225283051888 1101 118-119 1.2624026013719387 1101 120-121 1.284109633894893 1101 122-123 1.3326365737428425 1101 124-125 1.255211184347722 1102 1 0.45157425913418336 1102 2 -0.5059298108150756 1102 3 0.07492941319634383 1102 4 0.44562750429573583 1102 5 0.6813998068775362 1102 6 0.5528118665820472 1102 7 0.6741565861813967 1102 8 0.501209856027657 1102 9 0.705754685781713 1102 10-11 0.8297424596488767 1102 12-13 0.9076085820969126 1102 14-15 0.9894704883031551 1102 16-17 1.1168194310731963 1102 18-19 0.9924229362242869 1102 20-21 0.9052927322880322 1102 22-23 0.9269471632394968 1102 24-25 0.9310086037219847 1102 26-27 0.95667960748289 1102 28-29 1.1215047514535286 1102 30-31 0.9251483106332259 1102 32-33 0.8896264587695057 1102 34-35 0.824914527050808 1102 36-37 0.8624145732298274 1102 38-39 0.7095054257861051 1102 40-41 0.7683505192172007 1102 42-43 0.7344463629131326 1102 44-45 0.48572415053642004 1102 46-47 0.4361786031744259 1102 48-49 0.5864865453309918 1102 50-51 0.4954470575487875 1102 52-53 0.5395547934676443 1102 54-55 0.4905884912305254 1102 56-57 0.44007702074005906 1102 58-59 0.5592343884144455 1102 60-61 0.3462463274600971 1102 62-63 0.5702871811843764 1102 64-65 0.42928894330715295 1102 66-67 0.3699477699838276 1102 68-69 0.2524224244334512 1102 70-71 0.09524042366463092 1102 72-73 0.21589101484076778 1102 74-75 0.19644420808274532 1102 76-77 0.2521844468515724 1102 78-79 0.318885928110447 1102 80-81 0.2212305027691137 1102 82-83 0.2351420342515489 1102 84-85 0.3426944839158743 1102 86-87 0.3100329795121297 1102 88-89 0.37727089751230736 1102 90-91 0.31805645373306035 1102 92-93 0.37884938636795695 1102 94-95 0.3320176641854218 1102 96-97 0.2182946566979993 1102 98-99 0.1942596580230287 1102 100-101 0.15410377862572133 1102 102-103 0.1961922398858036 1102 104-105 0.1312150853192513 1102 106-107 0.34574733806484303 1102 108-109 0.36851592932919175 1102 110-111 0.3624739650541926 1102 112-113 0.289950020208682 1102 114-115 0.43246023795858335 1102 116-117 0.41173326308263825 1102 118-119 0.4260348280840134 1102 120-121 0.38204321026454124 1102 122-123 0.3526517102536566 1102 124-125 0.17082195200294237 1103 1 0.4807995534572811 1103 2 -0.8448009658790241 1103 3 0.1626857714641119 1103 4 0.4367938834715872 1103 5 0.7015356707201192 1103 6 0.7628527644090326 1103 7 0.7665509328470037 1103 8 0.8236223741779298 1103 9 0.9234071010917901 1103 10-11 1.0992498822227503 1103 12-13 1.1033865425607416 1103 14-15 1.1472330537169881 1103 16-17 1.3019520438169891 1103 18-19 1.294800055570036 1103 20-21 1.3196648755901208 1103 22-23 1.1859657260362368 1103 24-25 1.0944983032894697 1103 26-27 1.0557651691005248 1103 28-29 1.2145630008908128 1103 30-31 1.2199314311791056 1103 32-33 1.198080163897238 1103 34-35 1.2218900278177642 1103 36-37 1.3611666528819342 1103 38-39 1.3479970339680278 1103 40-41 1.4047158018341293 1103 42-43 1.4145843068688322 1103 44-45 1.5662977749233065 1103 46-47 1.4348434213434018 1103 48-49 1.4197066526485997 1103 50-51 1.5069425961856027 1103 52-53 1.4762226514047683 1103 54-55 1.4977104076195644 1103 56-57 1.4221338321700578 1103 58-59 1.4246466436000702 1103 60-61 1.396796100620815 1103 62-63 1.4402610433090253 1103 64-65 1.452915450882049 1103 66-67 1.3877854776401577 1103 68-69 1.3547287314175769 1103 70-71 1.2945826432117187 1103 72-73 1.2853804761541703 1103 74-75 1.189123058163755 1103 76-77 1.1810744520186596 1103 78-79 1.1382638783950085 1103 80-81 1.2463432954279376 1103 82-83 1.238205295876302 1103 84-85 1.2352840440346498 1103 86-87 1.1916109223466407 1103 88-89 1.1431965792011525 1103 90-91 1.2511043984964694 1103 92-93 1.2340410942404105 1103 94-95 1.129244548836141 1103 96-97 1.1727657012651633 1103 98-99 1.2608511234369075 1103 100-101 1.2206157276585259 1103 102-103 1.184551093330402 1103 104-105 1.2723029623535886 1103 106-107 1.2687844064920846 1103 108-109 1.2520106373960118 1103 110-111 1.2882287897917521 1103 112-113 1.3176465789123135 1103 114-115 1.266305043078905 1103 116-117 1.2385214688412312 1103 118-119 1.3754245324829704 1103 120-121 1.329841665006036 1103 122-123 1.232542327283813 1103 124-125 1.1347106349704639 1104 1 0.44467140270820593 1104 2 -0.6396543494503533 1104 3 0.08874285650536962 1104 4 0.32839225146782525 1104 5 0.7347417276443267 1104 6 0.6287618403647528 1104 7 0.7514339898786773 1104 8 0.7193598207105083 1104 9 0.7611244158739083 1104 10-11 0.9555620537512866 1104 12-13 0.7978777256903697 1104 14-15 0.8978752841903699 1104 16-17 1.018407399478157 1104 18-19 1.0029199030138045 1104 20-21 1.0007917067876804 1104 22-23 0.9898490089634251 1104 24-25 0.9539755708162971 1104 26-27 0.8464948628110562 1104 28-29 1.074986836450563 1104 30-31 0.9651670095045404 1104 32-33 0.9926196696612308 1104 34-35 0.9516672977570622 1104 36-37 1.025775696451987 1104 38-39 0.9861695391932415 1104 40-41 1.0122903930473015 1104 42-43 1.0010186959164855 1104 44-45 0.9080607761609869 1104 46-47 0.901428100363411 1104 48-49 0.9384292975415676 1104 50-51 0.9235130562156186 1104 52-53 0.8396690275361607 1104 54-55 1.0603315705940446 1104 56-57 1.0706623311930024 1104 58-59 1.0505804730262867 1104 60-61 0.9802361312080912 1104 62-63 1.0080626339351575 1104 64-65 0.9916294474124676 1104 66-67 0.9442531442417597 1104 68-69 0.9368312631288482 1104 70-71 0.9135017952774191 1104 72-73 0.8693807861208391 1104 74-75 0.8519297625089202 1104 76-77 0.8536078227819353 1104 78-79 0.90925132693798 1104 80-81 0.8079313336386384 1104 82-83 0.9081879845301337 1104 84-85 0.8390000984805823 1104 86-87 0.8371254706762201 1104 88-89 0.8058349584848514 1104 90-91 0.8252272257141726 1104 92-93 0.8732734076810509 1104 94-95 0.8674232680158411 1104 96-97 0.8875111594127176 1104 98-99 0.9073807395095557 1104 100-101 0.9073934194156799 1104 102-103 0.8737369848756948 1104 104-105 0.8686911926851408 1104 106-107 0.9326808556922721 1104 108-109 0.8792993647708194 1104 110-111 0.7794150629469563 1104 112-113 0.8229174378782105 1104 114-115 0.9017426041675627 1104 116-117 0.9232854372626456 1104 118-119 0.8442737592842704 1104 120-121 0.8003805391398302 1104 122-123 0.7964031376273404 1104 124-125 0.7679240024293748 1105 1 0.6193868249373935 1105 2 0.39044426743307525 1105 3 0.6033328675916572 1105 4 0.6219352195966081 1105 5 0.8114904592659755 1105 6 0.6378748740180455 1105 7 0.8238819758884759 1105 8 0.756307278815413 1105 9 0.9239701591347256 1105 10-11 1.0279036119520129 1105 12-13 1.0363582609555095 1105 14-15 1.0447983363820441 1105 16-17 1.1544806281261373 1105 18-19 1.0354717418968846 1105 20-21 1.202679734685404 1105 22-23 1.0127395997403923 1105 24-25 1.0916955957791146 1105 26-27 1.0652691732765405 1105 28-29 1.2502371965683992 1105 30-31 1.195602664528593 1105 32-33 1.1754819504974492 1105 34-35 1.0514158468080126 1105 36-37 1.1326257720351123 1105 38-39 1.0812408623313132 1105 40-41 0.8862756329526675 1105 42-43 0.8644525120287483 1105 44-45 1.1260654047300775 1105 46-47 1.0628780820202195 1105 48-49 0.9468804406465736 1105 50-51 0.9393255159194496 1105 52-53 0.8596299470724347 1105 54-55 0.9599589512614131 1105 56-57 0.8942122720297583 1105 58-59 0.7796559619913808 1105 60-61 0.8852109531011862 1105 62-63 0.8559701182429755 1105 64-65 0.916955448063522 1105 66-67 0.8645574459633778 1105 68-69 0.8453582477096653 1105 70-71 0.8916477094542685 1105 72-73 0.7645201139736066 1105 74-75 0.8472002915218688 1105 76-77 0.9059142562174038 1105 78-79 0.8479379288322377 1105 80-81 0.6572255371337796 1105 82-83 0.6880131759566588 1105 84-85 0.5313414873154088 1105 86-87 0.6632006140200559 1105 88-89 0.6630661073425479 1105 90-91 0.6696479972074236 1105 92-93 0.47769236494974265 1105 94-95 0.6223140671989817 1105 96-97 0.6164103930176665 1105 98-99 0.7376133851025131 1105 100-101 0.874009534667163 1105 102-103 0.44499840487384645 1105 104-105 0.4208044341704884 1105 106-107 0.7038983123216873 1105 108-109 0.773411804208159 1105 110-111 0.7065390692140667 1105 112-113 0.5350777946603991 1105 114-115 0.6075795128437456 1105 116-117 0.4935593679466379 1105 118-119 0.3396498549237599 1105 120-121 0.31927194172487106 1105 122-123 0.3473699298544197 1105 124-125 0.38557431179485135 1106 1 0.42021816049622274 1106 2 0.4637324235358662 1106 3 0.5744538345756887 1106 4 0.45527856265605493 1106 5 0.5728431569705776 1106 6 0.5687365835346867 1106 7 0.6067508579299741 1106 8 0.6391469102128085 1106 9 0.6937646705794549 1106 10-11 0.867811319496731 1106 12-13 0.6734997111757508 1106 14-15 0.890858202329575 1106 16-17 0.997524716520914 1106 18-19 0.8999760574790798 1106 20-21 0.9661104237271445 1106 22-23 0.897726247877948 1106 24-25 0.9093923466776204 1106 26-27 0.9294187691894606 1106 28-29 1.0659014169692185 1106 30-31 0.9767897735584228 1106 32-33 0.8248061410581684 1106 34-35 0.8834435364909297 1106 36-37 0.8239983890946974 1106 38-39 0.9496400040529096 1106 40-41 0.8874893817563674 1106 42-43 0.9724438324840108 1106 44-45 0.9091340642220018 1106 46-47 0.8238750868877318 1106 48-49 0.9372190390958437 1106 50-51 1.0662147822120858 1106 52-53 1.0842165036766005 1106 54-55 1.213475910239893 1106 56-57 1.0946787947788117 1106 58-59 1.1346262219828915 1106 60-61 1.1480170074284182 1106 62-63 1.2121900647354273 1106 64-65 1.09244749561417 1106 66-67 0.9920876127754639 1106 68-69 1.0219685345939737 1106 70-71 1.0742348455103539 1106 72-73 1.1508055240748654 1106 74-75 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-2.792388724546214 2216 78-79 -2.452644071219293 2216 80-81 -2.0989905436460887 2216 82-83 -2.1245485760195315 2216 84-85 -2.126116915712096 2216 86-87 -2.200747326862107 2216 88-89 -2.015360493055713 2216 90-91 -2.0047717905577045 2216 92-93 -2.04600080680002 2216 94-95 -1.8484908867358172 2216 96-97 -1.8772368765393388 2216 98-99 -1.9591755384267842 2216 100-101 -2.1414220837118023 2216 102-103 -1.9226249452737285 2216 104-105 -1.9130104484567205 2216 106-107 -2.052294434821622 2216 108-109 -2.1094145604086343 2216 110-111 -2.0450242187069705 2216 112-113 -2.0647166852979133 2216 114-115 -2.0973841366369186 2216 116-117 -2.2353003573290415 2216 118-119 -2.368234771483028 2216 120-121 -2.278558897691859 2216 122-123 -2.24447704776264 2216 124-125 -2.374722723426718 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92476.0 3 30142.0 4 14145.0 5 10732.0 6 11678.0 7 13909.0 8 15669.0 9 17123.0 10 18223.0 11 20750.0 12 24074.0 13 27177.0 14 30631.0 15 25641.0 16 15594.0 17 12819.0 18 11710.0 19 11063.0 20 10739.0 21 11186.0 22 13211.0 23 17479.0 24 24302.0 25 32428.0 26 38391.0 27 42314.0 28 48819.0 29 56815.0 30 66380.0 31 79071.0 32 95174.0 33 118267.0 34 151962.0 35 207632.0 36 326482.0 37 521810.0 38 203829.0 39 776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61778874873691 19.736345982111217 13.689030008363218 23.956835260788655 2 15.370232498604555 20.939780763189084 39.098594995191895 24.59139174301447 3 19.444771622380266 25.621513278229823 29.433102500867193 25.50061259852272 4 12.66384878996186 17.28221701752497 36.33652280707152 33.71741138544165 5 12.863172009924273 39.20828501836509 33.683940752353124 14.244602219357514 6 31.47771349794242 37.47074116183745 16.94667049296513 14.104874847255003 7 27.924274909020852 33.53642667167485 20.265309814819837 18.273988604484458 8 26.35109701119799 36.322148835394486 18.753900839545672 18.572853313861852 9 26.4955535218673 16.42155146488292 19.74765715469923 37.33523785855055 10-11 24.868255751509206 27.577618948492173 26.57475483743983 20.979370462558794 12-13 25.939233295795034 24.765540814599944 27.719030297534857 21.576195592070164 14-15 22.6475831411272 25.83311137539173 26.163012388896217 25.35629309458485 16-17 21.74329459246357 29.32121201321987 26.689933596915417 22.24555979740114 18-19 21.599375865167445 27.198415782273276 29.963996891468536 21.23821146109074 20-21 22.424903475372354 26.279229616484116 30.106110298362097 21.189756609781433 22-23 22.167683209455042 26.288840914528226 29.868971433035895 21.674504442980837 24-25 21.88561346672479 26.434567313588513 30.17052783852494 21.509291381161756 26-27 21.59924844867064 26.819611895461186 30.027122713582767 21.554016942285408 28-29 21.374020606866864 26.54952927371779 30.295435286399407 21.781014833015938 30-31 21.573404764709146 26.39370717426333 30.339918312101847 21.692969748925677 32-33 21.41239067365168 26.49834903679477 30.171653624580173 21.91760666497337 34-35 21.908664332842363 26.359990982031654 30.07838508748603 21.65295959763995 36-37 21.791804207969534 26.20834474240591 29.936232644296183 22.063618405328373 38-39 21.759633096591426 26.597441212196276 29.958071304282363 21.684854386929935 40-41 21.951851820370813 26.28784318773038 30.111231053867787 21.649073938031016 42-43 21.786188663680665 26.48965595822918 29.781441501282014 21.94271387680814 44-45 21.909696461175987 26.771708998094812 29.31238396145426 22.006210579274942 46-47 22.055777440705736 26.907186346760785 28.662675095041834 22.374361117491645 48-49 22.165362911867685 27.151741839752404 28.34375008221619 22.339145166163725 50-51 22.125885532665418 27.252498616236633 28.22110261908207 22.400513232015882 52-53 22.52358163610382 27.26945610200195 27.983548255811954 22.22341400608228 54-55 22.164207165561077 27.086710517954376 28.24961558279025 22.499466733694295 56-57 22.204291217329043 27.566370015797904 27.97963313083036 22.249705636042698 58-59 22.351188819821726 27.03011083239143 28.07911771223649 22.53958263555035 60-61 22.281161472227858 26.98944760086828 28.472170784453958 22.257220142449903 62-63 22.31690144550585 26.99159280877738 28.42617024127113 22.26533550444564 64-65 22.15861669790052 26.902586810544765 28.369127462448546 22.56966902910617 66-67 22.00839626280497 27.507495073105044 28.057720663978277 22.426388000111714 68-69 22.110232313119496 27.02055909685444 28.33618586830497 22.533022721721096 70-71 21.922304617094554 27.11931363061058 28.366934170045372 22.591447582249497 72-73 22.302311297032176 26.96218401935576 28.183818998151246 22.551685685460818 74-75 22.007951582170566 27.25551009834745 28.141800854253713 22.59473746522827 76-77 22.220472766026514 27.053046435109724 28.28707415863862 22.439406640225144 78-79 22.126561989308296 27.179732110422233 28.110859373656073 22.58284652661339 80-81 21.80381055267646 27.44811808855913 28.261224612050622 22.486846746713788 82-83 22.143649888408486 27.40031036768938 28.00175907404544 22.45428066985669 84-85 21.999412698146074 27.130229025461418 28.097131874361114 22.773226402031394 86-87 22.04755100966968 27.069772242482625 28.263849818465886 22.618826929381814 88-89 22.161596488011323 26.955083798701786 28.13834810086363 22.744971612423264 90-91 21.988806062276602 27.2455368544695 28.34600827402643 22.41964880922747 92-93 22.007335042994864 27.188354278407406 28.315184623813412 22.48912605478432 94-95 21.94894437424952 26.95711781595761 28.20923201220481 22.88470579758806 96-97 22.15772754208885 26.973415778583014 28.002807542477438 22.866049136850695 98-99 21.836401841894414 26.95459438433892 27.97958478650098 23.22941898726569 100-101 21.81303918782942 27.005108851483882 28.300981051673794 22.880870909012902 102-103 22.11504485313195 26.980525932974693 28.0345128083286 22.86991640556476 104-105 22.01094749214921 26.64712764430992 28.35923414102136 22.982690722519507 106-107 21.835361251897094 26.984185129289802 28.2205877263586 22.95986589245451 108-109 21.8168788268471 27.037662631514824 27.838702404616967 23.30675613702111 110-111 21.66027552731959 26.93339048525707 28.175837688536586 23.230496298886752 112-113 21.890768173395365 26.83525044099278 28.134813050633824 23.139168334978034 114-115 21.673835941492243 26.879812192320728 28.002347595990873 23.444004270196157 116-117 21.454512484651843 26.889404423709717 28.00493488657593 23.65114820506251 118-119 21.62844130925028 26.9073838450297 27.771833402513852 23.692341443206168 120-121 21.353634216948137 26.933294041474635 28.02137628235734 23.69169545921989 122-123 21.550165293353963 26.95715527456304 27.84653167287803 23.646147759204972 124-125 21.448192355104162 26.707835195550512 27.90957899907167 23.93439345027365 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 283.0 1 210.0 2 266.0 3 509.5 4 457.0 5 406.5 6 1028.0 7 2237.5 8 3384.0 9 3808.0 10 3153.0 11 2277.5 12 2156.0 13 2432.5 14 2735.0 15 3012.5 16 3286.5 17 3651.5 18 4058.0 19 4360.5 20 4741.5 21 5409.5 22 6141.0 23 7141.5 24 8371.5 25 10175.5 26 13094.0 27 16693.5 28 19624.0 29 22907.0 30 28196.5 31 33136.5 32 38323.5 33 44946.0 34 52792.0 35 59316.0 36 65328.0 37 72198.0 38 75028.5 39 75271.0 40 75573.0 41 78085.5 42 81694.0 43 84911.5 44 87748.0 45 89087.5 46 88483.5 47 88631.5 48 96511.0 49 99744.5 50 95352.5 51 90832.5 52 85190.5 53 81683.0 54 78761.5 55 72352.5 56 64515.5 57 58338.5 58 52851.5 59 48070.0 60 41837.0 61 32809.5 62 24027.5 63 17457.5 64 13001.0 65 10217.5 66 7740.5 67 5723.0 68 4053.0 69 2769.5 70 2136.0 71 1712.5 72 1466.0 73 1317.5 74 1057.5 75 854.0 76 611.0 77 362.5 78 232.0 79 141.0 80 90.0 81 58.0 82 36.0 83 22.5 84 18.0 85 17.5 86 12.0 87 6.0 88 4.0 89 3.0 90 1.5 91 1.5 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05982296772919219 2 0.07597274047881851 3 0.0 4 0.008297502289908254 5 0.029101971446068464 6 8.095124185276345E-5 7 8.095124185276345E-5 8 4.0475620926381724E-5 9 6.476099348221076E-4 10-11 0.05848727223862159 12-13 0.010402234578080103 14-15 0.00748798987138062 16-17 0.05302306341356006 18-19 1.2142686277914519E-4 20-21 0.010887942029196684 22-23 0.013033149938294917 24-25 0.0 26-27 0.0 28-29 0.05263854501475943 30-31 0.0 32-33 0.003865421798469455 34-35 0.0 36-37 6.0713431389572596E-5 38-39 0.0 40-41 0.0 42-43 0.003824946177543073 44-45 0.0 46-47 2.0237810463190862E-5 48-49 1.0118905231595431E-4 50-51 2.226159150950995E-4 52-53 1.4166467324233604E-4 54-55 0.0 56-57 0.0016999760789080326 58-59 2.0237810463190862E-5 60-61 0.0 62-63 0.0 64-65 5.261830720429625E-4 66-67 0.0 68-69 5.464208825061533E-4 70-71 0.0 72-73 0.00163926264751846 74-75 0.0018416407521503685 76-77 5.666586929693442E-4 78-79 6.273721243589168E-4 80-81 0.0014773601638129329 82-83 2.0237810463190863E-4 84-85 1.0118905231595431E-4 86-87 5.261830720429625E-4 88-89 0.0 90-91 0.0 92-93 0.006213007812199595 94-95 0.00491778794255538 96-97 0.005464208825061533 98-99 5.868965034325351E-4 100-101 3.6428058833743555E-4 102-103 1.619024837055269E-4 104-105 4.6546964065338986E-4 106-107 0.0027725800334571483 108-109 0.0033999521578160653 110-111 6.071343138957259E-4 112-113 0.0024690128765092853 114-115 4.2499401972700816E-4 116-117 0.0020035432358558956 118-119 0.0013559333010337879 120-121 0.008965350035193552 122-123 6.273721243589168E-4 124-125 0.0018821163730767501 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2470623.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.48819208396487 #Duplication Level Percentage of deduplicated Percentage of total 1 76.5127576442552 38.62990804817007 2 12.158690769856527 12.277406301560939 3 4.4937816473892855 6.8064873299035895 4 2.140653591540402 4.323109188596843 5 1.2235293625767731 3.0886892739073613 6 0.7385538296355927 2.23729485689938 7 0.4830979405748158 1.7073519133376402 8 0.3446045418126112 1.3918768240033452 9 0.2496763475705595 1.1345136655468664 >10 1.4404229634414731 13.60242575750586 >50 0.1292764249301138 4.548270421497989 >100 0.0797721759157868 7.276599413128842 >500 0.00445393480544577 1.6046393980242768 >1k 4.858837969576162E-4 0.4213267000442737 >5k 2.429418984788081E-4 0.9501009078727638 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9664 0.39115640063255297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8285 0.33534051937507264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5289 0.21407555907963297 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2528 0.102322369701893 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.0475620926381724E-5 0.0 0.0 0.0 0.0 3 4.0475620926381724E-5 0.0 0.0 0.0 0.0 4 4.0475620926381724E-5 0.0 0.0 0.0 0.0 5 4.0475620926381724E-5 0.0 0.0 0.0 0.0 6 4.0475620926381724E-5 0.0 0.0 0.0 0.0 7 4.0475620926381724E-5 0.0 0.0 0.0 0.0 8 4.0475620926381724E-5 0.0 0.0 0.0 0.0 9 4.0475620926381724E-5 0.0 0.0 0.0 0.0 10-11 4.0475620926381724E-5 0.0 0.0 0.0 0.0 12-13 4.0475620926381724E-5 0.0 0.0 2.0237810463190862E-5 0.0 14-15 8.095124185276345E-5 0.0 0.0 4.0475620926381724E-5 0.0 16-17 8.095124185276345E-5 0.0 0.0 4.0475620926381724E-5 0.0 18-19 1.0118905231595431E-4 0.0 0.0 4.0475620926381724E-5 0.0 20-21 1.2142686277914518E-4 0.0 0.0 1.4166467324233604E-4 0.0 22-23 1.2142686277914518E-4 0.0 0.0 3.4404277787424464E-4 0.0 24-25 2.0237810463190863E-4 0.0 0.0 6.273721243589168E-4 0.0 26-27 2.0237810463190863E-4 0.0 0.0 8.095124185276345E-4 0.0 28-29 2.833293464846721E-4 0.0 0.0 0.0014166467324233604 0.0 30-31 2.833293464846721E-4 0.0 0.0 0.0034606655892056378 0.0 32-33 2.833293464846721E-4 0.0 0.0 0.006739190884242557 0.0 34-35 3.4404277787424464E-4 0.0 0.0 0.01149507634309241 0.0 36-37 4.0475620926381725E-4 0.0 0.0 0.019428298044663228 0.0 38-39 4.45231830190199E-4 0.0 0.0 0.03418166187232937 0.0 40-41 4.45231830190199E-4 0.0 0.0 0.051545703249747124 0.0 42-43 4.45231830190199E-4 0.0 0.0 0.0677764272412262 0.0 44-45 4.6546964065338986E-4 0.0 0.0 0.0866178287824569 0.0 46-47 5.059452615797716E-4 0.0 0.0 0.10883894467104047 0.0 48-49 5.261830720429625E-4 0.0 0.0 0.1351278604627254 0.0 50-51 5.261830720429625E-4 0.0 0.0 0.16544410053658531 0.0 52-53 5.261830720429625E-4 0.0 0.0 0.19576034061044523 0.0 54-55 5.261830720429625E-4 0.0 0.0 0.22791822143645551 0.0 56-57 6.071343138957259E-4 0.0 0.0 0.2662283966432758 0.0 58-59 6.071343138957259E-4 0.0 0.0 0.31107538462970674 0.0 60-61 6.071343138957259E-4 0.0 0.0 0.3621556182388005 0.0 62-63 6.071343138957259E-4 0.0 0.0 0.4144905960966121 0.0 64-65 6.071343138957259E-4 0.0 0.0 0.4658541590521905 0.0 66-67 6.678477452852985E-4 0.0 0.0 0.5280651884160392 0.0 68-69 6.880855557484894E-4 0.0 0.0 0.6049081547447749 0.0 70-71 7.083233662116802E-4 0.0 0.0 0.691020038265652 0.0 72-73 7.48798987138062E-4 0.0 0.0 0.7807949654803668 0.0 74-75 7.690367976012528E-4 0.0 0.0 0.8674127942628236 0.0 76-77 7.690367976012528E-4 0.0 0.0 0.9635423939629801 0.0 78-79 7.690367976012528E-4 0.0 0.0 1.0774407912498183 0.0 80-81 8.499880394540162E-4 0.0 0.0 1.2160293173017496 0.0 82-83 8.90463660380398E-4 0.0 0.0 1.3622879735192297 0.0 84-85 8.90463660380398E-4 0.0 0.0 1.5111370694759985 0.0 86-87 9.107014708435888E-4 0.0 0.0 1.6675146309250743 0.0 88-89 9.309392813067797E-4 0.0 0.0 1.8493513579368441 0.0 90-91 0.001032128333622734 0.0 0.0 2.0564448724066766 0.0 92-93 0.001052366144085925 0.0 0.0 2.280396482992346 0.0 94-95 0.001052366144085925 0.0 0.0 2.4991267384785134 0.0 96-97 0.001052366144085925 0.0 0.0 2.7239283371036374 0.0 98-99 0.001052366144085925 0.0 0.0 2.978904511129379 0.0 100-101 0.0011130795754754974 0.0 0.0 3.2782217278799717 0.0 102-103 0.0012142686277914518 0.0 0.0 3.6084420812078575 0.0 104-105 0.0013964089219601697 0.0 0.0 3.9395933738170497 0.0 106-107 0.0014368845428865513 0.0 0.0 4.271837508191254 0.0 108-109 0.0014571223533497422 0.0 0.0 4.625452770414588 0.0 110-111 0.0014571223533497422 0.0 0.0 5.028428052357644 0.0 112-113 0.0014571223533497422 0.0 0.0 5.4719599064689355 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8100 0.0 46.76963 1 GTATCAA 13105 0.0 44.13408 1 ATCAACG 16110 0.0 35.565586 3 TCAACGC 16220 0.0 35.363934 4 TATCAAC 16300 0.0 35.209614 2 CAACGCA 16415 0.0 34.506134 5 AACGCAG 16810 0.0 33.721855 6 GTACATG 13865 0.0 33.036827 1 TACATGG 14160 0.0 31.196978 2 ACATGGG 14525 0.0 29.165073 3 CGCAGAG 19735 0.0 28.331573 8 ACGCAGA 19815 0.0 28.30734 7 GCAGAGT 20810 0.0 26.09652 9 GAGTACT 11535 0.0 24.45136 12-13 AGTACTT 11840 0.0 24.047882 12-13 CATGGGG 5800 0.0 23.800955 4 GTACTTT 12040 0.0 23.673153 14-15 CATGGGA 9335 0.0 22.7556 4 AGTACAT 10735 0.0 21.906193 2 CAGAGTA 20760 0.0 21.526598 10-11 >>END_MODULE