FastQCFastQC Report
Thu 26 May 2016
SRR1364603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315379
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48310.20864834655579065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139400.036.1481441
TACATGG137950.034.885852
GTATCAA97500.034.088511
ACATGGG141150.031.8163263
CGCATAG3100.030.7110568
ATCAACG114000.029.3101223
TCAACGC114400.028.9993844
CAACGCA114850.028.9401265
AACGCAG115250.027.939876
TATCAAC120450.027.6416932
CATGGGA97550.026.6817174
CATGGGG45700.026.456934
GTTATCA14700.025.5271111
ACCGTAT1401.0560501E-425.5011448
ACGCAGA131900.024.2744377
GGTATCA69450.024.2712751
AACGCAT3950.024.1096536
ATGGGAG38350.023.6088855
CGCAGAG129600.023.5072298
ACGCATA3950.022.5998657
Produced by FastQC (version 0.11.5)