FastQCFastQC Report
Thu 26 May 2016
SRR1364603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315379
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108780.46981509290703594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94580.4084860405143175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63120.272611956832985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29650.12805678897493672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.11635244165210101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94250.043.0543171
GTATCAA143250.040.473351
ATCAACG176750.032.248483
TATCAAC177450.032.2469332
TCAACGC177600.032.094834
GTACATG136350.032.0331571
CAACGCA178000.031.7882545
AACGCAG182100.031.3587746
TACATGG137800.031.1549472
ACATGGG137400.029.9222663
ACGCAGA211450.026.8652637
CGCAGAG211500.026.633688
GAGTACT119200.025.601112-13
CATGGGG46450.025.4905914
CATGGGA90950.024.794144
AGTACTT121650.024.20444912-13
GTACTTT126950.024.20237514-15
GCAGAGT225900.024.19899
AGAGTAC203950.021.80629510-11
GTATAGA9950.021.5590861
Produced by FastQC (version 0.11.5)