##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364603_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315379 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.62985455081004 31.0 30.0 33.0 26.0 34.0 2 29.787296161881056 31.0 30.0 34.0 26.0 34.0 3 29.80229284277002 31.0 30.0 34.0 26.0 34.0 4 33.460682246837344 35.0 33.0 37.0 28.0 37.0 5 32.88557294507724 35.0 33.0 37.0 26.0 37.0 6 32.87503816869722 35.0 33.0 37.0 26.0 37.0 7 32.674281402742274 35.0 33.0 37.0 25.0 37.0 8 32.59709187999027 35.0 33.0 37.0 25.0 37.0 9 33.90172494438276 38.0 33.0 39.0 24.0 39.0 10-11 33.687879824426155 38.0 33.0 39.0 22.5 39.0 12-13 33.550056599805046 37.0 33.0 39.0 21.5 39.0 14-15 34.51524480441431 38.0 33.0 41.0 21.5 41.0 16-17 34.44263358180237 38.0 33.0 41.0 20.5 41.0 18-19 34.50844634938815 38.5 33.5 41.0 20.0 41.0 20-21 34.5448840125094 39.0 34.0 41.0 21.0 41.0 22-23 34.53613123380665 39.0 34.0 41.0 22.0 41.0 24-25 34.51866670640098 39.0 33.5 41.0 22.0 41.0 26-27 34.40906132430155 39.0 33.0 41.0 21.5 41.0 28-29 34.16716572103314 39.0 33.0 41.0 18.5 41.0 30-31 34.073518633450504 38.0 33.0 41.0 18.5 41.0 32-33 33.85864841134 38.0 33.0 40.5 17.5 41.0 34-35 33.70538732535796 38.0 33.0 40.0 16.5 41.0 36-37 33.519290578345924 38.0 33.0 40.0 16.0 41.0 38-39 33.37083734455569 38.0 32.5 40.0 15.0 41.0 40-41 33.179810087247056 38.0 32.0 40.0 9.0 41.0 42-43 32.9815332608614 38.0 32.0 40.0 8.5 41.0 44-45 32.74226573705644 38.0 31.5 40.0 6.5 41.0 46-47 32.4251744530809 38.0 31.0 40.0 2.0 41.0 48-49 32.122837988942635 38.0 30.5 40.0 2.0 41.0 50-51 31.910073901508134 38.0 30.0 40.0 2.0 41.0 52-53 31.7402943535378 37.0 30.0 40.0 2.0 41.0 54-55 31.52744215957733 37.0 30.0 40.0 2.0 41.0 56-57 31.308986563322897 37.0 30.0 40.0 2.0 41.0 58-59 31.12145592579012 36.5 29.0 40.0 2.0 41.0 60-61 30.905762080419663 36.0 29.0 40.0 2.0 41.0 62-63 30.64583919090568 36.0 29.0 39.5 2.0 41.0 64-65 30.386950905229767 35.0 29.0 39.0 2.0 41.0 66-67 30.113603863557543 35.0 28.0 39.0 2.0 41.0 68-69 29.802169104928396 35.0 28.0 38.5 2.0 40.0 70-71 29.545099527982245 35.0 28.0 38.0 2.0 40.0 72-73 29.392550852365854 35.0 28.0 37.0 2.0 40.0 74-75 29.10821921594694 35.0 28.0 37.0 2.0 39.0 76-77 28.81293818420224 34.0 28.0 36.5 2.0 39.0 78-79 28.50388770045854 34.0 28.0 36.0 2.0 39.0 80-81 28.221074605928447 34.0 27.0 36.0 2.0 37.5 82-83 27.966812776655573 34.0 27.0 35.0 2.0 37.0 84-85 27.943789116166293 34.0 27.0 35.0 2.0 37.0 86-87 27.792940810122232 34.0 27.0 35.0 2.0 36.0 88-89 27.64056014155782 34.0 27.0 35.0 2.0 36.0 90-91 27.488680038991458 34.0 27.0 35.0 2.0 36.0 92-93 27.359084193127778 34.0 26.0 35.0 2.0 35.0 94-95 27.266473652909525 34.0 26.0 35.0 2.0 35.0 96-97 27.178971131724005 34.0 26.0 35.0 2.0 35.0 98-99 27.088176492919736 34.0 26.0 35.0 2.0 35.0 100-101 26.989550738777538 34.0 25.5 35.0 2.0 35.0 102-103 25.82425944089499 32.0 24.0 34.0 2.0 35.0 104-105 26.73598166865986 33.5 25.0 35.0 2.0 35.0 106-107 26.903427041534023 34.0 25.0 35.0 2.0 35.0 108-109 26.910380762717466 34.0 25.0 35.0 2.0 35.0 110-111 26.85440893261967 34.0 25.0 35.0 2.0 35.0 112-113 26.80232739434883 34.0 25.0 35.0 2.0 35.0 114-115 26.71922998351458 34.0 25.0 35.0 2.0 35.0 116-117 26.60059281871348 34.0 25.0 35.0 2.0 35.0 118-119 26.498219082059567 34.0 25.0 35.0 2.0 35.0 120-121 26.359713247809538 34.0 25.0 35.0 2.0 35.0 122-123 26.17703386788945 34.0 24.5 35.0 2.0 35.0 124-125 25.384032592504294 33.0 21.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6581777712834054 1101 2 0.6636329725463206 1101 3 0.5203141355423888 1101 4 0.6547780268110941 1101 5 0.9258291952147246 1101 6 0.8310413042127323 1101 7 0.8770693539058669 1101 8 0.8549411929425688 1101 9 1.090466492116576 1101 10-11 1.210133634971399 1101 12-13 1.123807919561031 1101 14-15 1.1980816699544121 1101 16-17 1.3004936604040367 1101 18-19 1.2264837832050617 1101 20-21 1.2552826362902039 1101 22-23 1.1984325368010502 1101 24-25 1.1291064690633732 1101 26-27 1.2500484202828375 1101 28-29 1.231714922631447 1101 30-31 1.331207543339879 1101 32-33 1.3972439094977815 1101 34-35 1.278356538573945 1101 36-37 1.3379241086156242 1101 38-39 1.4486000717553296 1101 40-41 1.4972540721346164 1101 42-43 1.510934815609609 1101 44-45 1.3811360709176483 1101 46-47 1.4029894695022094 1101 48-49 1.4538634651614686 1101 50-51 1.534349289550363 1101 52-53 1.4243540089580051 1101 54-55 1.4594542536868005 1101 56-57 1.4726635216716097 1101 58-59 1.5405916733742586 1101 60-61 1.5327205656600889 1101 62-63 1.5050865817345205 1101 64-65 1.5845722101607898 1101 66-67 1.5337611174345653 1101 68-69 1.5278641887118631 1101 70-71 1.4075022194331623 1101 72-73 1.398361649886862 1101 74-75 1.41104988417867 1101 76-77 1.3807163444357222 1101 78-79 1.315463529525573 1101 80-81 1.380182445639587 1101 82-83 1.293665583317896 1101 84-85 1.3890884446886602 1101 86-87 1.4459605670609896 1101 88-89 1.27572413037786 1101 90-91 1.3162221402316376 1101 92-93 1.3511416698280385 1101 94-95 1.2638373573689137 1101 96-97 1.247805451372983 1101 98-99 1.2990899597655208 1101 100-101 1.3734237030399399 1101 102-103 1.3810026126310682 1101 104-105 1.3306489834935178 1101 106-107 1.348104447691579 1101 108-109 1.3669500111267325 1101 110-111 1.3834329678903572 1101 112-113 1.3488454695551297 1101 114-115 1.3206901034409597 1101 116-117 1.2868885341151177 1101 118-119 1.2827357639498729 1101 120-121 1.358803841295348 1101 122-123 1.4773072343946794 1101 124-125 1.3185256202172475 1102 1 0.4891397018666588 1102 2 -0.47636884833114834 1102 3 0.19117678915393554 1102 4 0.35242515491832194 1102 5 0.8702942649790728 1102 6 0.8416478738153543 1102 7 0.7725936354235188 1102 8 0.754981350537598 1102 9 1.0904524148845525 1102 10-11 1.0486728231116018 1102 12-13 1.168703919219837 1102 14-15 1.2644676888140651 1102 16-17 1.3272326129630159 1102 18-19 1.3340921855966599 1102 20-21 1.2340017347875971 1102 22-23 1.312241418468247 1102 24-25 1.279461760907303 1102 26-27 1.3378038856015735 1102 28-29 1.3374207329714878 1102 30-31 1.2545842115022907 1102 32-33 1.0891365317037724 1102 34-35 1.0722072621061898 1102 36-37 1.0451335505899806 1102 38-39 0.977788962470278 1102 40-41 0.9695264230931642 1102 42-43 0.8929250879923813 1102 44-45 0.7347684674098076 1102 46-47 0.8870862372281252 1102 48-49 0.8403419589409538 1102 50-51 0.7241594393346134 1102 52-53 0.8002118975514563 1102 54-55 0.7960546154799886 1102 56-57 0.6291761864741403 1102 58-59 0.9245124007323398 1102 60-61 0.7085497333683186 1102 62-63 0.8756549712183777 1102 64-65 0.7900892189972133 1102 66-67 0.7947287180603269 1102 68-69 0.6734778689826086 1102 70-71 0.4767661159726728 1102 72-73 0.6315059762500788 1102 74-75 0.6207910205411515 1102 76-77 0.7099689325431591 1102 78-79 0.6887863501930482 1102 80-81 0.7265430355205922 1102 82-83 0.6358002964309897 1102 84-85 0.6908720633052923 1102 86-87 0.6081735529160355 1102 88-89 0.7791925437763538 1102 90-91 0.7688317216955376 1102 92-93 0.847182968928319 1102 94-95 0.6352003191385656 1102 96-97 0.5887391433619769 1102 98-99 0.46816284742479297 1102 100-101 0.35632982014701753 1102 102-103 0.48037737528602165 1102 104-105 0.5349430223854945 1102 106-107 0.7880887511613857 1102 108-109 0.7476329334701397 1102 110-111 0.5964628038756103 1102 112-113 0.7122469652901877 1102 114-115 0.7527774129329003 1102 116-117 0.7667496376484912 1102 118-119 0.8064449463284085 1102 120-121 0.7357696247087233 1102 122-123 0.6598653606601204 1102 124-125 0.5838542473680981 1103 1 0.258245346460793 1103 2 -0.8462835381964169 1103 3 0.1359156278903555 1103 4 0.25845597540622833 1103 5 0.5124625404246501 1103 6 0.5758336403335065 1103 7 0.5446638743712242 1103 8 0.5801274328947414 1103 9 0.6240592683552322 1103 10-11 0.8080418189785235 1103 12-13 0.8632455718302197 1103 14-15 0.8731780399448326 1103 16-17 0.9563235267065124 1103 18-19 1.0202402387488405 1103 20-21 1.0797898587536352 1103 22-23 0.9854790368129756 1103 24-25 0.9143687164362575 1103 26-27 0.9251385100523137 1103 28-29 1.071565855307064 1103 30-31 0.9497062272149108 1103 32-33 1.0163138422701152 1103 34-35 0.971981821236227 1103 36-37 1.0584800254001863 1103 38-39 1.1035957153753984 1103 40-41 0.992250871315342 1103 42-43 1.1000295887491873 1103 44-45 1.083577695217251 1103 46-47 1.0880102633137554 1103 48-49 1.0449872520844607 1103 50-51 1.0620885649217229 1103 52-53 1.0821653687053896 1103 54-55 0.9971419433232995 1103 56-57 0.9857640625661013 1103 58-59 1.0871554923514921 1103 60-61 1.0331230440864836 1103 62-63 1.0504984825857413 1103 64-65 0.9928274768587464 1103 66-67 1.0452131003305922 1103 68-69 0.9588127825913269 1103 70-71 0.8833267182401343 1103 72-73 0.9699508095556162 1103 74-75 0.980021280284344 1103 76-77 0.9457217391455117 1103 78-79 0.9367922902998167 1103 80-81 0.9715330322750297 1103 82-83 0.9344460305617375 1103 84-85 0.9631729036120369 1103 86-87 0.8275899996932665 1103 88-89 0.8644612240004648 1103 90-91 0.8754201308958791 1103 92-93 0.8624868470998344 1103 94-95 0.807756198492303 1103 96-97 0.8643678593939121 1103 98-99 0.8640562496328883 1103 100-101 0.8386642292219761 1103 102-103 0.8256337982823538 1103 104-105 0.8788554151933923 1103 106-107 0.9133049479321151 1103 108-109 0.8438100346315025 1103 110-111 0.8354698319499505 1103 112-113 0.8779253627578782 1103 114-115 0.7514604646499592 1103 116-117 0.7674969702940437 1103 118-119 0.8142112264986139 1103 120-121 0.7311781541868427 1103 122-123 0.7858047082804731 1103 124-125 0.6923753663497827 1104 1 0.38965597050354006 1104 2 -0.6703318544482464 1104 3 0.1359885813148658 1104 4 0.44093138208994276 1104 5 0.7979027236769056 1104 6 0.744961184847746 1104 7 0.8092651860147626 1104 8 0.8684913195158401 1104 9 0.9330274792167756 1104 10-11 1.014461678165759 1104 12-13 0.9090708226550532 1104 14-15 0.8458714888116532 1104 16-17 1.1505097291476218 1104 18-19 1.1905129411798399 1104 20-21 1.2091954865410486 1104 22-23 1.0673353092893763 1104 24-25 1.073999285354894 1104 26-27 0.9347835009774101 1104 28-29 1.1950497313351605 1104 30-31 1.1161221393266985 1104 32-33 1.0740138021246395 1104 34-35 1.137203827362626 1104 36-37 1.155323798804318 1104 38-39 1.1811688206105018 1104 40-41 1.2214776577886752 1104 42-43 1.4048104417223115 1104 44-45 1.305362473337631 1104 46-47 1.3761884771440407 1104 48-49 1.364188551245789 1104 50-51 1.3760717122589625 1104 52-53 1.172052126435922 1104 54-55 1.3934157944544552 1104 56-57 1.3612125363635919 1104 58-59 1.2610360157269511 1104 60-61 1.3799719621829816 1104 62-63 1.3103142604294895 1104 64-65 1.4216145186437714 1104 66-67 1.3671291524454823 1104 68-69 1.2371806584574934 1104 70-71 1.2862535421600825 1104 72-73 1.287551561186735 1104 74-75 1.321010520286773 1104 76-77 1.3443657331810428 1104 78-79 1.2099449662145574 1104 80-81 1.2549929728327491 1104 82-83 1.3123134796714524 1104 84-85 1.3176579789438279 1104 86-87 1.3067865927261764 1104 88-89 1.2451342172330833 1104 90-91 1.2209250191970185 1104 92-93 1.2742463487562574 1104 94-95 1.2416654562397014 1104 96-97 1.1642222055625524 1104 98-99 1.1718788808815788 1104 100-101 1.2389749770746974 1104 102-103 1.2774367599383716 1104 104-105 1.2175951595443522 1104 106-107 1.1550495299806798 1104 108-109 1.1638300145925058 1104 110-111 1.068130574067606 1104 112-113 1.111810499488623 1104 114-115 1.1022861456048147 1104 116-117 1.0935700181784789 1104 118-119 1.0184128798846643 1104 120-121 1.0331408964934177 1104 122-123 0.9645131459133083 1104 124-125 0.9256699598684826 1105 1 0.640662778536214 1105 2 0.48865351003247426 1105 3 0.7040145144848609 1105 4 0.6691617118819906 1105 5 0.9070619530423727 1105 6 0.6842327454553185 1105 7 0.7209567726663977 1105 8 0.8752105066504896 1105 9 0.954853569585012 1105 10-11 1.132507852878902 1105 12-13 1.118587128405629 1105 14-15 1.1127726909501732 1105 16-17 1.2958122725295382 1105 18-19 1.2151399358270538 1105 20-21 1.2885257176302432 1105 22-23 1.1570083447329154 1105 24-25 1.1681337692920053 1105 26-27 1.2372827438448013 1105 28-29 1.335017894476323 1105 30-31 1.2937685934169068 1105 32-33 1.249732243151307 1105 34-35 1.258284531213853 1105 36-37 1.2964644231355464 1105 38-39 1.3147716791022575 1105 40-41 1.2018108950348037 1105 42-43 1.2591191585722328 1105 44-45 1.3461606937679562 1105 46-47 1.3357107815898175 1105 48-49 1.2613689294164985 1105 50-51 1.2575997923133357 1105 52-53 1.3021424651644011 1105 54-55 1.3342134960787106 1105 56-57 1.3039625700859894 1105 58-59 1.2290416100776653 1105 60-61 1.229953512142064 1105 62-63 1.279040670965923 1105 64-65 1.328317663321922 1105 66-67 1.3360474806966813 1105 68-69 1.255814429684765 1105 70-71 1.154072698685173 1105 72-73 1.1308696956638649 1105 74-75 1.1786832486619652 1105 76-77 1.2553374764083571 1105 78-79 1.3050912841181663 1105 80-81 1.041599159141196 1105 82-83 1.1342988759290442 1105 84-85 1.0013470240942226 1105 86-87 1.041033735401733 1105 88-89 1.0925567562440577 1105 90-91 1.0914240522722025 1105 92-93 0.9799107220839183 1105 94-95 1.0450355232045965 1105 96-97 1.1165615183520288 1105 98-99 1.1009126953643111 1105 100-101 1.1556599505701932 1105 102-103 0.7628603764447028 1105 104-105 0.6039254382988553 1105 106-107 1.0235455257705688 1105 108-109 1.114515754623973 1105 110-111 1.0577462246958156 1105 112-113 0.8367113102528094 1105 114-115 0.9520407179075718 1105 116-117 0.7232612410173616 1105 118-119 0.6852387416539756 1105 120-121 0.569440460307213 1105 122-123 0.5682847768839565 1105 124-125 0.4205657187585494 1106 1 0.3818308172665503 1106 2 0.4526570498513358 1106 3 0.4880684658837886 1106 4 0.38564470587859034 1106 5 0.32804119622846883 1106 6 0.5174987670588322 1106 7 0.5688855609420571 1106 8 0.5719900444051049 1106 9 0.6301042319347729 1106 10-11 0.7682727492587844 1106 12-13 0.7098523872382927 1106 14-15 0.769286828170074 1106 16-17 0.819027636623801 1106 18-19 0.8726552390636044 1106 20-21 0.9526482403328203 1106 22-23 0.9095705560344953 1106 24-25 0.78458354332993 1106 26-27 0.8121818836001182 1106 28-29 0.9942066482516267 1106 30-31 0.9100354429820143 1106 32-33 0.9368725668331521 1106 34-35 0.9068877900488559 1106 36-37 0.9123788522694909 1106 38-39 0.8267892626488944 1106 40-41 0.9263474063457906 1106 42-43 0.8993352951964297 1106 44-45 0.8898992390304414 1106 46-47 0.7691057403537371 1106 48-49 0.8828669660818136 1106 50-51 1.0205716787034191 1106 52-53 1.126535986218478 1106 54-55 1.0593359434410843 1106 56-57 0.962515889681125 1106 58-59 0.8393174684583684 1106 60-61 0.9608710271017777 1106 62-63 0.9281040125658606 1106 64-65 0.9407688629684081 1106 66-67 0.7894503013873653 1106 68-69 0.8091995278593096 1106 70-71 0.9165323887441303 1106 72-73 0.9066681555225635 1106 74-75 0.9625128579897826 1106 76-77 0.9951363922119612 1106 78-79 0.9135709225476099 1106 80-81 0.827633738346595 1106 82-83 0.7840121732422105 1106 84-85 0.8060505564571372 1106 86-87 0.7738873376592856 1106 88-89 0.7289056368563678 1106 90-91 0.9113359627789599 1106 92-93 0.9331100461299755 1106 94-95 0.8627103759300958 1106 96-97 0.7460546739084855 1106 98-99 0.8244447616027983 1106 100-101 1.0177016000445889 1106 102-103 0.9439508420823479 1106 104-105 0.9687346499474643 1106 106-107 0.8368252335286179 1106 108-109 0.8411372379829594 1106 110-111 0.7980663151977581 1106 112-113 0.7567503232101558 1106 114-115 0.7426456373501509 1106 116-117 0.9520791452868878 1106 118-119 1.0294568488345988 1106 120-121 0.8486211882029622 1106 122-123 0.8550378987207878 1106 124-125 0.890247607401033 1107 1 0.4686546730224457 1107 2 0.5877472024172192 1107 3 0.6655996725412088 1107 4 0.49549213999786446 1107 5 0.5377499340925525 1107 6 0.5358066727453803 1107 7 0.4177066427728491 1107 8 0.277953983378751 1107 9 0.4220598491331131 1107 10-11 0.6473296147234109 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120-121 -2.4864950848778236 2216 122-123 -2.46685771487218 2216 124-125 -2.5906457877876434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86460.0 3 28396.0 4 13495.0 5 10278.0 6 11519.0 7 13556.0 8 15182.0 9 16185.0 10 17435.0 11 19739.0 12 22829.0 13 26291.0 14 30008.0 15 24617.0 16 15210.0 17 12793.0 18 11686.0 19 10706.0 20 10570.0 21 10658.0 22 12966.0 23 16801.0 24 23036.0 25 30331.0 26 36138.0 27 40180.0 28 46195.0 29 53609.0 30 62259.0 31 73598.0 32 88860.0 33 110734.0 34 143428.0 35 195594.0 36 303998.0 37 476169.0 38 192999.0 39 871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03076215680818 19.258845675821906 13.769721159613693 23.940671007756226 2 15.437469528324396 21.09188874250959 39.00460921912982 24.466032510036204 3 19.595668786837923 25.389968553744335 29.442436853750507 25.571925805667234 4 12.503374404970923 17.288917741401153 36.51868524111932 33.6890226125086 5 12.833287107959146 39.175097397295424 33.53180003335139 14.459815461394035 6 31.367141003691845 37.42150289456727 17.022189090997042 14.189167010743848 7 27.884851680869527 33.64149022686998 20.156786426757776 18.31687166550271 8 26.525203865112363 35.902847870694174 18.81782636881478 18.754121895378685 9 26.697927369613545 16.23487766734172 19.52126811861791 37.54592684442682 10-11 24.97099160106915 27.525135210470246 26.46458382400923 21.03928936445137 12-13 26.212032666128483 24.691438811911464 27.562639810883038 21.53388871107702 14-15 22.735208342691678 25.700256217109015 26.451263463060158 25.113271977139146 16-17 21.599157348889968 29.191249392318912 26.94398530762167 22.265607951169446 18-19 21.46075689683097 26.94306537817848 30.37369756518922 21.22248015980133 20-21 22.447688190342248 25.97105767386733 30.41730233297454 21.16395180281588 22-23 21.888468003560117 26.07603105446929 30.327889564719523 21.70761137725107 24-25 21.645570768327776 26.12973945086312 30.738207438177508 21.486482342631593 26-27 21.529844574041658 26.478991128450247 30.48179153391302 21.509372763595074 28-29 21.238345128104744 26.40797247901075 30.723899503967093 21.629782888917408 30-31 21.499763105737767 26.100219445714934 30.778352053810632 21.621665394736674 32-33 21.304004459020867 26.339989975409306 30.547489068941847 21.808516496627984 34-35 21.728494557478495 26.236136718869783 30.51487467062628 21.520494053025445 36-37 21.829484925323047 26.043924672180246 30.34839310987906 21.778197292617648 38-39 21.6878748576367 26.323681781686716 30.426681765706608 21.56176159496998 40-41 21.89041662086782 26.177383093088235 30.341345918172774 21.59085436787117 42-43 21.822455740268044 26.23907813640506 30.020062108313788 21.918404015013106 44-45 21.856918456978317 26.570336864936582 29.632038642485742 21.940706035599355 46-47 21.978580594002615 26.709473230493348 29.022487482600024 22.289458692904006 48-49 22.16077787848143 26.842115605920334 28.7329760261245 22.264130489473732 50-51 22.173650619178165 27.103040877959977 28.453647207192812 22.269661295669046 52-53 22.38609522336967 27.121414784719722 28.29860968645711 22.193880305453494 54-55 21.97355637533993 26.956154252965554 28.56355883066203 22.50673054103249 56-57 22.205495577420887 27.27530298537857 28.198193392790543 22.32100804441 58-59 22.284201542901418 26.896839306584063 28.34485341054463 22.47410573996989 60-61 22.267412808011127 26.818395606075722 28.761770751138364 22.152420834774784 62-63 22.248884523872768 26.813169679780284 28.74244346173996 22.19550233460699 64-65 22.032888104947553 26.78129505227251 28.67339332096956 22.512423521810373 66-67 22.07880437716676 27.125796683825843 28.430960978742576 22.364437960264823 68-69 22.126470663182122 26.88131082459066 28.637317539724805 22.354900972502413 70-71 21.908400309409387 26.938095231925313 28.541072541471614 22.61243191719369 72-73 22.15903458972201 26.892427482689808 28.49293609346241 22.455601834125773 74-75 22.053848349923573 27.109030858409454 28.33860331004351 22.498517481623466 76-77 22.137066645935636 26.867476904339266 28.463809239991882 22.53164720973322 78-79 22.131492351726017 26.93156871137478 28.392033373658254 22.54490556324095 80-81 21.925587186852212 27.329025687582455 28.390035936398988 22.355351189166345 82-83 22.165535964784986 27.22061125277277 28.195075011574794 22.418777770867454 84-85 21.99641786197237 27.014045660814634 28.260365374623657 22.72917110258934 86-87 22.036161006008985 26.89970963566467 28.39564958816464 22.668479770161706 88-89 22.122711254337034 26.823432972190076 28.39270123653649 22.6611545369364 90-91 21.87477730427718 27.18779517305806 28.465534152292133 22.471893370372626 92-93 21.942953400605774 27.08410135138783 28.41613890733572 22.556806340670672 94-95 21.851633731586382 26.938803012132883 28.369196840150025 22.840366416130706 96-97 21.998297365797697 26.903931175914682 28.24286595405393 22.854905504233695 98-99 21.915775930260505 26.86291264920199 28.105333102699603 23.1159783178379 100-101 21.71548317742599 26.945357784994282 28.411429529004206 22.927729508575514 102-103 21.959154798183967 26.698255488525408 28.392710298456926 22.949879414833703 104-105 21.797181055904442 26.574839750268907 28.49939135011006 23.128587843716588 106-107 21.73888725123391 26.845520185807253 28.30792088722748 23.107671675731357 108-109 21.871704606634637 26.82925038392041 27.97359344539713 23.325451564047817 110-111 21.612444368814273 26.914879993849773 28.219039756443976 23.25363588089198 112-113 21.74407483047112 26.70212910692224 28.19251043109281 23.36128563151383 114-115 21.431711935208572 26.722901067994375 28.14317205731443 23.702214939482623 116-117 21.441830934816785 26.726802342656736 28.253394779123404 23.57797194340307 118-119 21.381120904712215 26.80612470544334 28.03121264054067 23.781541749303774 120-121 21.230691282536824 26.861761634039492 28.14792242044346 23.75962466298022 122-123 21.305120831284512 26.810860444169183 28.01464914798218 23.86936957656413 124-125 21.188905814656085 26.726388662740096 28.002638058616903 24.082067463986913 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 202.5 2 364.5 3 712.0 4 599.0 5 495.5 6 1331.0 7 3036.5 8 4525.5 9 5045.0 10 4073.0 11 2644.0 12 2334.0 13 2433.5 14 2610.0 15 3007.5 16 3266.5 17 3496.0 18 3887.5 19 4264.5 20 4670.5 21 5246.0 22 6114.5 23 7104.5 24 8276.5 25 10008.5 26 12681.0 27 15949.5 28 19102.5 29 22737.5 30 27467.0 31 32131.5 32 36886.0 33 41763.0 34 46885.0 35 51718.5 36 56736.5 37 62709.5 38 66290.5 39 67935.0 40 70320.0 41 74389.0 42 78267.5 43 80706.5 44 82363.5 45 83452.5 46 83482.5 47 85044.5 48 92343.0 49 93923.5 50 88821.0 51 84684.5 52 80110.0 53 77144.5 54 74088.5 55 67908.0 56 60248.5 57 53636.0 58 48254.0 59 42939.5 60 36856.5 61 30131.5 62 23465.5 63 17497.5 64 13224.5 65 10437.5 66 7661.0 67 5753.0 68 4234.0 69 2816.0 70 2075.5 71 1661.0 72 1464.0 73 1398.0 74 1127.0 75 851.5 76 621.5 77 396.0 78 244.0 79 148.5 80 96.5 81 68.0 82 48.5 83 34.5 84 23.5 85 14.5 86 11.5 87 9.0 88 5.5 89 3.0 90 1.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05904001029637049 2 0.07579752602057806 3 0.0 4 0.006737557868495826 5 0.027338936735627296 6 3.023263146119922E-4 7 0.0 8 0.0 9 5.182736821919867E-4 10-11 0.060206126081302457 12-13 0.008551515756167782 14-15 0.006694368394979828 16-17 0.05415959978906261 18-19 1.7275789406399555E-4 20-21 0.009631252594067753 22-23 0.012330594688817685 24-25 0.0 26-27 0.0 28-29 0.052604778742486646 30-31 0.0 32-33 0.00313123682990992 34-35 0.0 36-37 2.5913684109599334E-4 38-39 0.0 40-41 2.1594736757999444E-5 42-43 0.0029800736726039237 44-45 0.0 46-47 8.637894703199778E-5 48-49 1.9435263082199502E-4 50-51 4.966789454339872E-4 52-53 8.637894703199778E-5 54-55 2.1594736757999444E-5 56-57 0.0019219315714619507 58-59 4.318947351599889E-5 60-61 0.0 62-63 0.0 64-65 5.614631557079856E-4 66-67 0.0 68-69 5.182736821919867E-4 70-71 0.0 72-73 0.0022674473595899417 74-75 0.0026777473579919315 76-77 3.8870526164399004E-4 78-79 8.63789470319978E-4 80-81 0.0016627947303659572 82-83 1.2956842054799667E-4 84-85 8.637894703199778E-5 86-87 5.182736821919867E-4 88-89 2.1594736757999444E-5 90-91 0.0 92-93 0.007191047340413815 94-95 0.006154499976029843 96-97 0.006176094712787843 98-99 8.421947335619783E-4 100-101 3.455157881279911E-4 102-103 1.2956842054799667E-4 104-105 3.671105248859906E-4 106-107 0.0033039947239739153 108-109 0.0052043315586778665 110-111 7.990052600459795E-4 112-113 0.002828910515297927 114-115 4.750842086759878E-4 116-117 0.002548178937443935 118-119 0.0015116315730599613 120-121 0.010063147329227742 122-123 9.71763154109975E-4 124-125 0.001727578940639956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2315379.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.67909597381162 #Duplication Level Percentage of deduplicated Percentage of total 1 76.22776181072717 40.91837342106877 2 12.437104346780524 13.352250357342829 3 4.610230327733395 7.424189886713338 4 2.2266823948419954 4.781051919036806 5 1.21087696407527 3.2499390383537023 6 0.7594977853938452 2.446149270843216 7 0.48754134565603746 1.831954507927019 8 0.353242988793278 1.5169411438008344 9 0.26318820131524084 1.27149342458181 >10 1.2776701596242293 12.18359662332241 >50 0.09236893394597566 3.4600938586289347 >100 0.04977247309190864 4.7898651525929665 >500 0.0031685690565933502 1.087023491801613 >1k 6.499628834036225E-4 0.5249113238644695 >5k 1.6249072085090563E-4 0.6877576917034467 >10k+ 8.124536042545281E-5 0.4744088884178879 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10878 0.46981509290703594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9458 0.4084860405143175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6312 0.272611956832985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2965 0.12805678897493672 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2694 0.11635244165210101 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.1594736757999446E-4 0.0 5 0.0 0.0 0.0 2.5913684109599334E-4 0.0 6 0.0 0.0 0.0 2.5913684109599334E-4 0.0 7 0.0 0.0 0.0 2.5913684109599334E-4 0.0 8 0.0 0.0 0.0 3.023263146119922E-4 0.0 9 0.0 0.0 0.0 3.023263146119922E-4 0.0 10-11 0.0 0.0 0.0 3.023263146119922E-4 0.0 12-13 0.0 0.0 0.0 3.023263146119922E-4 0.0 14-15 0.0 0.0 0.0 3.023263146119922E-4 0.0 16-17 0.0 4.3189473515998895E-5 0.0 3.4551578812799116E-4 0.0 18-19 0.0 8.637894703199779E-5 0.0 3.4551578812799116E-4 0.0 20-21 0.0 8.637894703199779E-5 0.0 3.8870526164399004E-4 0.0 22-23 0.0 8.637894703199779E-5 0.0 5.182736821919867E-4 0.0 24-25 0.0 1.0797368378999723E-4 0.0 7.774105232879801E-4 0.0 26-27 0.0 1.2956842054799667E-4 0.0 0.0010581421011419728 0.0 28-29 0.0 1.2956842054799667E-4 0.0 0.0015548210465759602 0.0 30-31 0.0 1.2956842054799667E-4 0.0 0.003735889459133904 0.0 32-33 0.0 1.2956842054799667E-4 0.0 0.007471778918267809 0.0 34-35 0.0 1.2956842054799667E-4 0.0 0.01250335258288168 0.0 36-37 0.0 1.2956842054799667E-4 0.0 0.02131400518014545 0.0 38-39 0.0 1.2956842054799667E-4 0.0 0.037941952483805025 0.0 40-41 0.0 1.2956842054799667E-4 0.0 0.0581330313525345 0.0 42-43 0.0 1.2956842054799667E-4 0.0 0.07700683127902602 0.0 44-45 0.0 1.2956842054799667E-4 0.0 0.09812648382834949 0.0 46-47 0.0 1.2956842054799667E-4 0.0 0.12388900478064283 0.0 48-49 0.0 1.2956842054799667E-4 0.0 0.15364655203316607 0.0 50-51 0.0 1.2956842054799667E-4 0.0 0.18575792559231125 0.0 52-53 0.0 1.2956842054799667E-4 0.0 0.2185171412541964 0.0 54-55 0.0 1.2956842054799667E-4 0.0 0.2539541042740735 0.0 56-57 0.0 1.2956842054799667E-4 0.0 0.29422828832774245 0.0 58-59 0.0 1.2956842054799667E-4 0.0 0.3425789039289032 0.0 60-61 0.0 1.2956842054799667E-4 0.0 0.3991787089716198 0.0 62-63 0.0 1.2956842054799667E-4 0.0 0.4569878192727843 0.0 64-65 0.0 1.2956842054799667E-4 0.0 0.5114065559029428 0.0 66-67 0.0 1.2956842054799667E-4 0.0 0.5757588714417812 0.0 68-69 0.0 1.2956842054799667E-4 0.0 0.6544500921879313 0.0 70-71 0.0 1.2956842054799667E-4 0.0 0.7485167655057768 0.0 72-73 0.0 1.2956842054799667E-4 0.0 0.8455203230227103 0.0 74-75 0.0 1.2956842054799667E-4 0.0 0.9424590963293697 0.0 76-77 0.0 1.2956842054799667E-4 0.0 1.048186927496535 0.0 78-79 0.0 1.2956842054799667E-4 0.0 1.1759413901568598 0.0 80-81 0.0 1.2956842054799667E-4 0.0 1.3228935737950462 0.0 82-83 0.0 1.2956842054799667E-4 0.0 1.4805567468652 0.0 84-85 0.0 1.2956842054799667E-4 0.0 1.639321251510012 0.0 86-87 0.0 1.2956842054799667E-4 0.0 1.805384777179028 0.0 88-89 0.0 1.2956842054799667E-4 0.0 1.9950945395980528 0.0 90-91 0.0 1.2956842054799667E-4 0.0 2.218751228200653 0.0 92-93 0.0 1.2956842054799667E-4 0.0 2.459575732525863 0.0 94-95 0.0 1.2956842054799667E-4 0.0 2.697592921072533 0.0 96-97 0.0 1.2956842054799667E-4 0.0 2.9362579517219425 0.0 98-99 0.0 1.2956842054799667E-4 0.0 3.20409747173141 0.0 100-101 0.0 1.5116315730599614E-4 0.0 3.5267876231061956 0.0 102-103 0.0 1.7275789406399558E-4 0.0 3.8725625480752828 0.0 104-105 0.0 1.7275789406399558E-4 0.0 4.219222857251448 0.0 106-107 0.0 1.7275789406399558E-4 0.0 4.559447934873729 0.0 108-109 0.0 1.7275789406399558E-4 0.0 4.921332533464284 0.0 110-111 0.0 1.7275789406399558E-4 0.0 5.358820305444595 0.0 112-113 0.0 2.1594736757999446E-4 0.0 5.832544045704829 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9425 0.0 43.054317 1 GTATCAA 14325 0.0 40.47335 1 ATCAACG 17675 0.0 32.24848 3 TATCAAC 17745 0.0 32.246933 2 TCAACGC 17760 0.0 32.09483 4 GTACATG 13635 0.0 32.033157 1 CAACGCA 17800 0.0 31.788254 5 AACGCAG 18210 0.0 31.358774 6 TACATGG 13780 0.0 31.154947 2 ACATGGG 13740 0.0 29.922266 3 ACGCAGA 21145 0.0 26.865263 7 CGCAGAG 21150 0.0 26.63368 8 GAGTACT 11920 0.0 25.6011 12-13 CATGGGG 4645 0.0 25.490591 4 CATGGGA 9095 0.0 24.79414 4 AGTACTT 12165 0.0 24.204449 12-13 GTACTTT 12695 0.0 24.202375 14-15 GCAGAGT 22590 0.0 24.1989 9 AGAGTAC 20395 0.0 21.806295 10-11 GTATAGA 995 0.0 21.559086 1 >>END_MODULE