FastQCFastQC Report
Thu 26 May 2016
SRR1364602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3236356
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34460.10647777932959168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84850.040.8638881
GTACATG113250.035.245621
ATCAACG95250.034.8379063
TCAACGC94150.034.5472834
CAACGCA94850.034.167775
TACATGG116600.033.772622
AACGCAG95200.033.0117456
TATCAAC104550.032.4227642
ACATGGG120950.031.3265863
GGTATCA54450.030.9641
ACGCAGA111000.028.0930677
CGCAGAG110150.026.8457378
CGCATAG3900.025.9350938
CATGGGA74000.022.7822364
GCAGAGT122400.022.6517249
CATGGGG58800.021.7822514
GAGTACA94800.021.5553151
AGTACAT87200.021.5206742
GTACCTG30700.020.3760261
GAGTACT50750.020.1633312-13
Produced by FastQC (version 0.11.5)