##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364602_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3236356 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.808752807169544 30.0 24.0 31.0 2.0 33.0 2 24.68314703326828 30.0 22.0 31.0 2.0 34.0 3 25.25124522765728 30.0 25.0 31.0 2.0 34.0 4 28.38639568700106 35.0 27.0 37.0 2.0 37.0 5 28.608770790358044 35.0 27.0 37.0 2.0 37.0 6 28.66262735001959 35.0 28.0 37.0 2.0 37.0 7 28.405091405271854 35.0 27.0 37.0 2.0 37.0 8 28.166315139619993 35.0 26.0 37.0 2.0 37.0 9 29.081763563711778 35.0 27.0 39.0 2.0 39.0 10-11 28.982011404184213 35.0 26.0 39.0 2.0 39.0 12-13 28.95916224914688 35.0 26.0 39.0 2.0 39.0 14-15 29.47093521231904 36.0 26.0 40.0 2.0 41.0 16-17 29.836681131494807 36.0 27.0 40.0 2.0 41.0 18-19 29.643459341308557 36.0 26.0 40.0 2.0 41.0 20-21 29.533594573650117 36.0 25.0 40.0 2.0 41.0 22-23 29.575794659178406 36.0 25.5 40.0 2.0 41.0 24-25 29.63800243236529 36.0 26.0 40.0 2.0 41.0 26-27 29.42008033108842 36.0 25.0 40.0 2.0 41.0 28-29 29.29072821407781 36.0 25.0 40.0 2.0 41.0 30-31 29.141741823211042 36.0 24.5 40.0 2.0 41.0 32-33 29.016542370493234 36.0 24.0 40.0 2.0 41.0 34-35 29.052331851007736 36.0 24.5 40.0 2.0 41.0 36-37 28.99419995822462 36.0 24.0 40.0 2.0 41.0 38-39 28.707343845979864 35.0 24.0 40.0 2.0 41.0 40-41 28.55907492871612 35.0 23.0 40.0 2.0 41.0 42-43 28.059590168695905 35.0 20.0 40.0 2.0 41.0 44-45 27.919802704028854 35.0 19.5 39.5 2.0 41.0 46-47 27.870232910100125 35.0 20.0 39.0 2.0 41.0 48-49 27.80269877603082 35.0 20.0 39.0 2.0 41.0 50-51 27.578761730786105 34.5 18.0 39.0 2.0 41.0 52-53 27.480590516000092 34.0 17.5 39.0 2.0 40.0 54-55 27.309861924955104 34.0 16.5 39.0 2.0 40.0 56-57 27.086265077142315 34.0 14.5 39.0 2.0 40.0 58-59 26.88042029368833 34.0 13.0 39.0 2.0 40.0 60-61 26.59622550794783 33.0 11.5 38.0 2.0 40.0 62-63 26.42646467199529 33.0 10.0 38.0 2.0 40.0 64-65 26.20809623539561 33.0 8.0 38.0 2.0 40.0 66-67 25.94886131191995 33.0 8.0 38.0 2.0 40.0 68-69 25.72015223294347 32.5 7.0 37.0 2.0 40.0 70-71 25.306978435005295 32.0 6.5 36.5 2.0 39.0 72-73 25.08776970147907 32.0 2.0 36.0 2.0 39.0 74-75 25.06213825055093 32.0 2.0 36.0 2.0 39.0 76-77 24.82474764828097 32.0 2.0 35.5 2.0 38.5 78-79 24.547155504524223 32.0 2.0 35.0 2.0 37.5 80-81 24.15439447947012 31.0 2.0 35.0 2.0 37.0 82-83 24.254670067199037 32.0 2.0 35.0 2.0 37.0 84-85 24.094794732099928 32.0 2.0 35.0 2.0 36.0 86-87 23.969756417402785 32.0 2.0 35.0 2.0 36.0 88-89 23.81340356252526 32.0 2.0 35.0 2.0 36.0 90-91 23.681482197879344 32.0 2.0 35.0 2.0 35.0 92-93 23.55640680444302 32.0 2.0 35.0 2.0 35.0 94-95 23.458212260950276 32.0 2.0 35.0 2.0 35.0 96-97 23.344081120865567 32.0 2.0 35.0 2.0 35.0 98-99 23.259215457137593 31.5 2.0 35.0 2.0 35.0 100-101 23.15214580843393 31.0 2.0 35.0 2.0 35.0 102-103 22.139568391116427 29.5 2.0 34.0 2.0 35.0 104-105 22.896373730207678 31.0 2.0 34.0 2.0 35.0 106-107 23.042560058287776 31.5 2.0 35.0 2.0 35.0 108-109 22.99892178116375 32.0 2.0 35.0 2.0 35.0 110-111 22.930298150141702 32.0 2.0 35.0 2.0 35.0 112-113 22.840278078184227 31.5 2.0 35.0 2.0 35.0 114-115 22.737223747943673 31.0 2.0 35.0 2.0 35.0 116-117 22.624292568555497 31.0 2.0 35.0 2.0 35.0 118-119 22.499326093915503 31.0 2.0 35.0 2.0 35.0 120-121 22.314888875018696 31.0 2.0 35.0 2.0 35.0 122-123 22.101284747413448 31.0 2.0 35.0 2.0 35.0 124-125 21.231458004002032 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1135969137328878 1101 2 1.2453924539672876 1101 3 0.9559418192831046 1101 4 1.0711197329110043 1101 5 1.1289455755476183 1101 6 0.982725571014889 1101 7 0.9531638243128562 1101 8 1.1350878030729348 1101 9 1.4498489297528003 1101 10-11 1.4957125132483 1101 12-13 1.4913235146197579 1101 14-15 1.811439173752163 1101 16-17 1.4220839604382398 1101 18-19 1.3900642408008146 1101 20-21 1.5574105447791204 1101 22-23 1.2954312346857009 1101 24-25 1.3544604912956189 1101 26-27 1.5008801455645155 1101 28-29 1.478899172385617 1101 30-31 1.6621193220066068 1101 32-33 1.6879277526917846 1101 34-35 1.433695007271396 1101 36-37 1.3915858835720556 1101 38-39 1.3500061164712847 1101 40-41 1.3924911118324061 1101 42-43 1.5478369493382615 1101 44-45 1.4347966186609078 1101 46-47 1.1565287947248315 1101 48-49 1.0068304539224435 1101 50-51 0.9772200679063445 1101 52-53 0.9225422576624993 1101 54-55 0.8760999332169774 1101 56-57 0.9144985489070585 1101 58-59 0.7819592508744009 1101 60-61 0.9411687406088731 1101 62-63 0.8331419127191744 1101 64-65 0.8305366459343375 1101 66-67 0.7359634408396971 1101 68-69 0.6157490277860553 1101 70-71 0.4999549505296912 1101 72-73 0.48476686820263026 1101 74-75 0.5389124020265648 1101 76-77 0.6494882050187236 1101 78-79 0.6580781586858109 1101 80-81 0.7761748818053995 1101 82-83 0.704050099980364 1101 84-85 0.7837179343911949 1101 86-87 0.6960074974994477 1101 88-89 0.723155322172051 1101 90-91 0.8229331127548285 1101 92-93 0.8081261596411657 1101 94-95 0.8325515412467048 1101 96-97 0.8069856708508674 1101 98-99 0.8508968998464042 1101 100-101 0.7191643738064926 1101 102-103 0.6236763667149994 1101 104-105 0.8376803598106832 1101 106-107 0.8601396739155476 1101 108-109 0.8572957862851425 1101 110-111 0.916010821493181 1101 112-113 0.8252676747278365 1101 114-115 0.7949156256316385 1101 116-117 0.78852328398251 1101 118-119 0.7752307145650867 1101 120-121 0.8834093662327156 1101 122-123 0.8278325618495863 1101 124-125 0.7755885842005021 1102 1 0.10030632686945395 1102 2 0.5593720205141715 1102 3 0.450247055945308 1102 4 0.21956316256489217 1102 5 0.32654194746826093 1102 6 0.014648494733993545 1102 7 0.15137571649244563 1102 8 0.13821135901921622 1102 9 0.7402181781413049 1102 10-11 0.6576968997196886 1102 12-13 0.9247563525979423 1102 14-15 0.9832467177119746 1102 16-17 0.7069539546864725 1102 18-19 0.5847728241179588 1102 20-21 0.8389965031461593 1102 22-23 0.7154462709116558 1102 24-25 0.5549846761532002 1102 26-27 0.9117652976208497 1102 28-29 0.5252697964378825 1102 30-31 0.5770704192004814 1102 32-33 0.7951298158473321 1102 34-35 0.6961238275121886 1102 36-37 0.5468756635721341 1102 38-39 0.870915993065406 1102 40-41 0.7468158228941313 1102 42-43 1.0327727297319917 1102 44-45 0.6273451207985836 1102 46-47 0.3303428267502504 1102 48-49 0.33581640353216713 1102 50-51 0.401463761044905 1102 52-53 0.290924012007892 1102 54-55 0.35873372990866415 1102 56-57 0.45447543979643257 1102 58-59 0.4211885178233139 1102 60-61 0.39243872142354874 1102 62-63 0.23164660107139312 1102 64-65 0.0738737741044524 1102 66-67 0.06423845370997938 1102 68-69 0.026386339307155282 1102 70-71 0.21747927439903236 1102 72-73 -0.08272606111520275 1102 74-75 0.07969156505674846 1102 76-77 -0.0659728782782274 1102 78-79 -0.06546244103727616 1102 80-81 0.0872692954457257 1102 82-83 0.08086330006079478 1102 84-85 0.02144357874470515 1102 86-87 0.12292378733938847 1102 88-89 0.08005200582762484 1102 90-91 0.147652357891225 1102 92-93 0.289818247321449 1102 94-95 0.22396312275414587 1102 96-97 0.06508499751532071 1102 98-99 0.19603516553230804 1102 100-101 0.22038355368813 1102 102-103 0.1155809298291004 1102 104-105 0.2697911194482998 1102 106-107 0.16561622705310697 1102 108-109 0.20023974924254873 1102 110-111 0.1894289463308425 1102 112-113 0.06552735039257129 1102 114-115 0.2076452766696164 1102 116-117 0.24743455409511128 1102 118-119 0.2787477758140078 1102 120-121 0.3137301475553187 1102 122-123 0.13332720352021 1102 124-125 0.03523121770386339 1103 1 0.7989685100158361 1103 2 1.0756952868554492 1103 3 0.9091263336964381 1103 4 0.5745030289101507 1103 5 0.7062373267799806 1103 6 0.8214491009081328 1103 7 0.8037824693574329 1103 8 0.9224406587625147 1103 9 1.5532087561613537 1103 10-11 1.6103647624034778 1103 12-13 1.505206215944579 1103 14-15 1.7543285350953255 1103 16-17 1.3732941268635308 1103 18-19 1.3862242900451953 1103 20-21 1.6358978461436955 1103 22-23 1.48561267717184 1103 24-25 1.3847498145528974 1103 26-27 1.5995046897449754 1103 28-29 1.5430364060141173 1103 30-31 1.6030372168595015 1103 32-33 1.7167365289823202 1103 34-35 1.5694013793070027 1103 36-37 1.268995358604922 1103 38-39 1.2554231026083613 1103 40-41 1.390287914455655 1103 42-43 1.6747802063828985 1103 44-45 1.3218407573603415 1103 46-47 1.2237410249707033 1103 48-49 1.0658771667598934 1103 50-51 1.1592076373754985 1103 52-53 0.855634422160108 1103 54-55 0.989050490891195 1103 56-57 0.9141789949796184 1103 58-59 0.871987389326609 1103 60-61 0.964963084354018 1103 62-63 0.8283692963024905 1103 64-65 0.7224435626983059 1103 66-67 0.6302658636364384 1103 68-69 0.7366309498536516 1103 70-71 0.7907028578941322 1103 72-73 0.7371600565089302 1103 74-75 0.823098846625804 1103 76-77 0.7176876614596637 1103 78-79 0.6209020975011725 1103 80-81 0.7249226830973114 1103 82-83 0.7657241541391571 1103 84-85 0.7368005106005491 1103 86-87 0.7228767752207084 1103 88-89 0.7146104075562576 1103 90-91 0.7944784266042255 1103 92-93 0.7170532485600098 1103 94-95 0.7071270632154096 1103 96-97 0.625763953377966 1103 98-99 0.7125780533475492 1103 100-101 0.613925936129526 1103 102-103 0.5366317060050996 1103 104-105 0.5262122492213024 1103 106-107 0.5700815720625343 1103 108-109 0.6096103598151217 1103 110-111 0.5257931932414763 1103 112-113 0.5420084251199704 1103 114-115 0.6128770452996548 1103 116-117 0.576553479573569 1103 118-119 0.6257255596073215 1103 120-121 0.5468956319285283 1103 122-123 0.3437464476620953 1103 124-125 0.3064554180775616 1104 1 0.6406618099525083 1104 2 1.0482128668230786 1104 3 0.5801490177741151 1104 4 0.7509967944978548 1104 5 0.4056759349576069 1104 6 0.5610669398573087 1104 7 0.29219008950353853 1104 8 0.5623003656475127 1104 9 1.0381779968695142 1104 10-11 1.086217278221845 1104 12-13 1.0068201410338418 1104 14-15 1.154682198296065 1104 16-17 0.8975097850159237 1104 18-19 0.8744046357133932 1104 20-21 1.0661696888578653 1104 22-23 1.0064945507072594 1104 24-25 0.9746115852860733 1104 26-27 1.055998204991667 1104 28-29 1.1040110840621722 1104 30-31 1.1564883310361047 1104 32-33 1.1732821218585023 1104 34-35 1.0974980767480318 1104 36-37 0.9339869173402846 1104 38-39 0.9234533114848666 1104 40-41 1.02745685311357 1104 42-43 1.2990315695274077 1104 44-45 1.0575247467880793 1104 46-47 0.947425662940752 1104 48-49 0.8309538288819063 1104 50-51 0.7928631780166455 1104 52-53 0.7541252007357357 1104 54-55 0.7734434742092766 1104 56-57 0.7494942923730257 1104 58-59 0.6934391947458316 1104 60-61 0.7455533960059775 1104 62-63 0.5683039338539118 1104 64-65 0.5456585977611184 1104 66-67 0.5431436525107145 1104 68-69 0.4365504388431667 1104 70-71 0.5832702707947135 1104 72-73 0.514593957270737 1104 74-75 0.5592045643811261 1104 76-77 0.5383351521719284 1104 78-79 0.5272803972720652 1104 80-81 0.6360499058994797 1104 82-83 0.6128397442054947 1104 84-85 0.5931085767388034 1104 86-87 0.5256888290652917 1104 88-89 0.46716553951360495 1104 90-91 0.46243206796920333 1104 92-93 0.562514850601854 1104 94-95 0.5497592466211003 1104 96-97 0.6347112375881956 1104 98-99 0.6153853096870421 1104 100-101 0.639499526843565 1104 102-103 0.5146710636317238 1104 104-105 0.5332053036395763 1104 106-107 0.5462907401031458 1104 108-109 0.5423097987163388 1104 110-111 0.6079640944389908 1104 112-113 0.6090333389909368 1104 114-115 0.6479993875404446 1104 116-117 0.5595457588556805 1104 118-119 0.6068849491369406 1104 120-121 0.5615113400486536 1104 122-123 0.5249349027046968 1104 124-125 0.35489265800338643 1105 1 0.8161102944717804 1105 2 1.1394341783572948 1105 3 0.683692679382176 1105 4 0.8258561821971107 1105 5 0.7701426085253225 1105 6 0.720004232889881 1105 7 0.6232685698104348 1105 8 0.7547629846778037 1105 9 0.9425349437495392 1105 10-11 1.0011950633334123 1105 12-13 1.065011997727055 1105 14-15 1.3528255563262732 1105 16-17 0.885588666061988 1105 18-19 1.1424049882818643 1105 20-21 1.0937461393337848 1105 22-23 1.144231799053827 1105 24-25 0.7544596733707962 1105 26-27 1.1766149743370704 1105 28-29 1.2139507272095855 1105 30-31 1.173484898618046 1105 32-33 1.2401966250290535 1105 34-35 0.9730654096392897 1105 36-37 0.6770207895362645 1105 38-39 0.8556226017460844 1105 40-41 0.972654901278112 1105 42-43 1.214261851059483 1105 44-45 0.8884287671960571 1105 46-47 0.41425005616939004 1105 48-49 0.368160195202595 1105 50-51 0.698025928337696 1105 52-53 0.4222898256141576 1105 54-55 0.4835618613725039 1105 56-57 0.6150170110039106 1105 58-59 0.6533299395159808 1105 60-61 0.492527569340524 1105 62-63 0.5348510688763604 1105 64-65 0.20587038482205955 1105 66-67 0.2201799455402096 1105 68-69 0.12299594395387459 1105 70-71 0.46227151810817446 1105 72-73 0.23608122227206252 1105 74-75 0.3573119276301675 1105 76-77 0.39007455487584153 1105 78-79 0.37214994181304206 1105 80-81 0.48826810478704274 1105 82-83 0.37531077773331845 1105 84-85 0.37382615606926706 1105 86-87 0.36562803150211565 1105 88-89 0.4337260803372409 1105 90-91 0.28779403997485886 1105 92-93 0.4299676151383842 1105 94-95 0.2111752804922702 1105 96-97 0.29327868219446174 1105 98-99 0.38427712753622956 1105 100-101 0.42870884080066674 1105 102-103 0.21398089035047008 1105 104-105 0.2784252848427613 1105 106-107 0.292000299525796 1105 108-109 0.34149306975232463 1105 110-111 0.3637023820426215 1105 112-113 0.3218475220826136 1105 114-115 0.36228277674836207 1105 116-117 0.31523364146677935 1105 118-119 0.3012601496346363 1105 120-121 0.3464907957608254 1105 122-123 0.3328649465605267 1105 124-125 0.37063594715975157 1106 1 0.18453813076864733 1106 2 -1.5360677704895025 1106 3 -0.10294519750224751 1106 4 0.09057307596080122 1106 5 0.28809539328244327 1106 6 0.23196109250848806 1106 7 0.4084435231025374 1106 8 0.3321533419126972 1106 9 0.854254962110577 1106 10-11 1.0643497590878255 1106 12-13 1.0565953350869322 1106 14-15 1.057342914753022 1106 16-17 0.743431667124991 1106 18-19 0.7675847130273858 1106 20-21 1.003009362500901 1106 22-23 0.7774283507224276 1106 24-25 0.8637877037470361 1106 26-27 0.7166374223141325 1106 28-29 0.8786867470523916 1106 30-31 0.9260895686025812 1106 32-33 1.0778379365546336 1106 34-35 0.9405854215578593 1106 36-37 0.7127630223348689 1106 38-39 1.0228978602501435 1106 40-41 1.014866057218068 1106 42-43 1.2103760760304922 1106 44-45 1.0333180372643476 1106 46-47 0.8045224920668232 1106 48-49 0.6914070786583366 1106 50-51 0.7101250658521927 1106 52-53 0.6620281003210238 1106 54-55 0.6454391363692835 1106 56-57 0.5771792041813377 1106 58-59 0.565363786636194 1106 60-61 0.5852886101213528 1106 62-63 0.523096623572922 1106 64-65 0.500196341528671 1106 66-67 0.4488031094366143 1106 68-69 0.384587877982991 1106 70-71 0.47112355070706613 1106 72-73 0.31694584184428365 1106 74-75 0.4156414961458239 1106 76-77 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-1.2449078971105223 2216 12-13 -2.158816767031894 2216 14-15 -1.5188249997057603 2216 16-17 -1.9851319758236023 2216 18-19 -1.3382775856422136 2216 20-21 -1.202110902272171 2216 22-23 -1.2154540459881993 2216 24-25 -1.3886834619711053 2216 26-27 -1.2258815181310467 2216 28-29 -1.2337633289283296 2216 30-31 -1.1877352873609155 2216 32-33 -1.1395733234240062 2216 34-35 -1.4288644629292477 2216 36-37 -1.4790545457702464 2216 38-39 -1.416084422890279 2216 40-41 -1.4089462930715442 2216 42-43 -1.0970581315744887 2216 44-45 -1.100617329169939 2216 46-47 -1.4197233900495831 2216 48-49 -1.4766037051148437 2216 50-51 -4.569741057447491 2216 52-53 -4.486376828804751 2216 54-55 -3.318266329031122 2216 56-57 -1.7385949781316832 2216 58-59 -1.6021421293138332 2216 60-61 -1.6298053188835482 2216 62-63 -1.8155949847514101 2216 64-65 -1.87077350424034 2216 66-67 -1.9804032281694006 2216 68-69 -1.9634118309220447 2216 70-71 -4.11157725239433 2216 72-73 -2.205716509894639 2216 74-75 -1.779314092599904 2216 76-77 -1.796372806038793 2216 78-79 -1.752479188057979 2216 80-81 -1.6794206951874315 2216 82-83 -1.7739077992771897 2216 84-85 -1.6507431983031289 2216 86-87 -1.742764460107363 2216 88-89 -1.8386070882959942 2216 90-91 -1.698067343138515 2216 92-93 -1.6590264604928002 2216 94-95 -1.8931614503042944 2216 96-97 -1.8856646393778007 2216 98-99 -1.8685059914452467 2216 100-101 -1.9661303155351781 2216 102-103 -1.6701670960699886 2216 104-105 -1.7201379566098396 2216 106-107 -1.7461560585586469 2216 108-109 -1.8531923836837976 2216 110-111 -1.7762062432466124 2216 112-113 -2.0112479533704644 2216 114-115 -1.8659440220522612 2216 116-117 -1.6813333768071956 2216 118-119 -1.6942452782165667 2216 120-121 -1.8982913214830823 2216 122-123 -1.7171896171953307 2216 124-125 -1.5526690712940692 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 429721.0 3 51874.0 4 29772.0 5 23586.0 6 22376.0 7 24682.0 8 27096.0 9 27957.0 10 29541.0 11 31080.0 12 32666.0 13 32955.0 14 31627.0 15 26847.0 16 21857.0 17 19043.0 18 17451.0 19 16385.0 20 16292.0 21 17422.0 22 21710.0 23 30430.0 24 42970.0 25 54189.0 26 60342.0 27 67152.0 28 76967.0 29 86549.0 30 99202.0 31 114053.0 32 134055.0 33 166757.0 34 210885.0 35 278754.0 36 381247.0 37 392723.0 38 88066.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73366254410068 18.898531122988956 14.084164256108142 23.28364207680222 2 13.371797131446508 20.15344218055414 42.826060761691 23.648699926308353 3 19.23085982268873 25.64409066763527 27.875729628511625 27.24931988116438 4 11.654492161535794 16.97115582757145 38.25076330165266 33.123588709240096 5 13.486747784942851 38.93013894343719 33.732818059825995 13.850295211793965 6 31.31700439742733 37.564120114993436 17.491899231456813 13.626976256122422 7 28.3933125531312 32.91577824334515 20.35363506434585 18.337274139177804 8 24.862680404199534 37.35396440279754 19.80238363286654 17.980971560136386 9 25.47146501863939 17.897867307382683 19.869369480945718 36.76129819303222 10-11 23.90447838415445 28.402976130890593 27.3530311415655 20.33951434338946 12-13 24.919167876388602 25.430764183151382 27.72625411279664 21.923813827663373 14-15 21.828246477893273 26.82427691497551 25.561508567027257 25.785968040103963 16-17 21.79043300344864 30.184387562365973 25.828000058183996 22.19717937600139 18-19 21.806464834583792 28.795604462281243 27.946621279420086 21.45130942371488 20-21 22.57270899191806 27.758518108526093 28.11885914242518 21.54991375713067 22-23 22.26273903050506 27.666727250940575 27.8552300071122 22.21530371144216 24-25 22.141410213582507 27.638217791803264 28.01244460901308 22.207927385601145 26-27 22.100071399918534 27.70324583100367 27.99978006966526 22.196902699412536 28-29 21.876673420708723 27.855687602958902 28.093332689501644 22.174306286830735 30-31 22.076418753490206 27.586858377098356 28.065432998701944 22.271289870709495 32-33 21.954031155385135 27.550440235099583 27.994893554575484 22.5006350549398 34-35 22.413849926634864 27.396985151663834 27.910022762301445 22.27914215939986 36-37 22.25550312069026 27.45503403847817 27.787457915127934 22.502004925703634 38-39 22.100530054615206 27.535412431092514 28.140521355806804 22.22353615848548 40-41 22.321582834053224 27.409750282538354 28.055965816729366 22.212701066679053 42-43 22.004079772439912 27.328061974866397 28.20570257256793 22.462155680125765 44-45 22.25850549013132 27.57576681462502 27.76682143457954 22.39890626066412 46-47 22.31481970055237 27.834588446357976 27.290000354352085 22.560591498737562 48-49 22.353680843988492 27.75456903303018 27.354808140613578 22.53694198236775 50-51 22.339022247147263 27.73453035433691 27.462387276343918 22.464060122171908 52-53 22.587686746491904 27.87087832783367 27.07949312589648 22.46194179977795 54-55 22.42377128933814 27.78108023992538 27.174658427311638 22.62049004342484 56-57 22.334960176996628 27.97912650628514 27.25753962800499 22.428373688713247 58-59 22.559802179283224 27.653326682371933 27.182431807273954 22.604439331070893 60-61 22.486669652177653 27.684770272976184 27.413551970947903 22.41500810389826 62-63 22.473102524418035 27.77757233969241 27.349545956903697 22.39977917898586 64-65 22.35397594797857 27.702773578979777 27.256172748778134 22.687077724263517 66-67 22.339687427630604 27.883424958049925 27.1093034886903 22.667584125629173 68-69 22.342069982923977 27.779355739774886 27.228987347529422 22.64958692977172 70-71 22.335403738238817 27.65423882259045 27.365638333075754 22.644719106094986 72-73 22.59573482813304 27.733639659623936 27.007300511937554 22.66332500030547 74-75 22.42555825508513 27.858835844885732 27.057406558433883 22.658199341595257 76-77 22.556414590093556 27.68234716254317 27.14340634052576 22.61783190683751 78-79 22.53680481765765 27.786283587548404 27.050713260626193 22.626198334167754 80-81 22.352900400534377 27.857950327300614 27.283189841782267 22.505959430382745 82-83 22.49252335523099 27.941004445137647 27.047149281610793 22.51932291802057 84-85 22.499411320409095 27.720078754654125 27.18933885016101 22.59117107477577 86-87 22.425470039917066 27.68126330684885 27.372112651731733 22.521154001502346 88-89 22.531982361730602 27.674290563501113 27.16982714545506 22.623899929313225 90-91 22.529313530882312 27.797834772893147 27.22022116453872 22.452630531685813 92-93 22.498292964041074 27.79150472454018 27.19997982347709 22.510222487941665 94-95 22.355585599974248 27.748460739414604 27.265774248383977 22.630179412227168 96-97 22.52372517558773 27.723077927698814 27.100913447693664 22.652283449019787 98-99 22.350121032105847 27.75627333759571 26.99265080427088 22.90095482602757 100-101 22.2207559791262 27.778455034260585 27.26522697752533 22.735562009087886 102-103 22.237083069781995 27.63937603218362 27.323222763778194 22.800318134256184 104-105 22.25398866432536 27.501479689501895 27.450568469943814 22.79396317622893 106-107 22.177907058415467 27.693221419839283 27.41651121120356 22.71236031054169 108-109 22.260780066027984 27.682520408038535 27.063243064345954 22.99345646158752 110-111 22.15577374704122 27.798516178272692 27.224270796691798 22.821439277994287 112-113 22.234360670931228 27.8008043960742 27.285508945321762 22.679325987672815 114-115 22.187472981047176 27.859183288687777 27.10454731972761 22.848796410537435 116-117 21.94757781295132 27.791350680973046 27.294110659299715 22.966960846775923 118-119 22.014569732042816 27.801278814502307 27.231975156758782 22.9521762966961 120-121 22.0891879783917 27.916773433412885 27.20520818626327 22.78883040193215 122-123 22.105831254511934 27.94902735598513 27.078822689205744 22.866318700297196 124-125 22.213197671181177 27.760074121279242 27.080407357681572 22.946320849858008 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5252.0 1 3717.5 2 1669.0 3 1047.5 4 885.0 5 839.0 6 862.0 7 996.5 8 1204.5 9 1351.0 10 1475.0 11 1596.5 12 1709.0 13 1838.0 14 2029.5 15 2186.5 16 2352.5 17 2620.0 18 2914.5 19 3323.5 20 3919.5 21 4719.5 22 5827.0 23 7408.0 24 9446.0 25 11998.5 26 15650.5 27 20333.0 28 25209.5 29 31088.0 30 38382.0 31 44883.0 32 51395.0 33 59286.0 34 67999.0 35 75869.0 36 83627.5 37 90857.0 38 95892.5 39 100248.0 40 104413.5 41 109446.0 42 114181.5 43 119313.5 44 124344.0 45 126143.0 46 126064.0 47 126328.0 48 126954.0 49 124151.5 50 118750.5 51 112977.5 52 107359.0 53 102371.0 54 97706.5 55 91318.5 56 82240.0 57 74669.0 58 68801.0 59 61130.0 60 51486.5 61 41733.5 62 32933.0 63 25772.5 64 19783.5 65 15101.5 66 11612.5 67 8989.0 68 6951.0 69 5225.0 70 3980.5 71 3135.0 72 2456.0 73 1973.5 74 1527.5 75 1076.5 76 747.0 77 512.0 78 368.0 79 275.5 80 191.5 81 127.5 82 95.5 83 80.0 84 63.0 85 54.0 86 37.0 87 24.0 88 24.5 89 21.0 90 15.0 91 11.5 92 6.0 93 3.0 94 3.5 95 3.0 96 1.0 97 1.0 98 1.5 99 1.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21054544061283742 2 0.2483966535201937 3 0.30015239361800744 4 0.2860315737823651 5 0.3203294075188267 6 0.23637696223777607 7 0.22621120791408605 8 0.20591059821601826 9 0.20504542763527867 10-11 0.14554950073477702 12-13 0.1726015308575447 14-15 0.19007488669355285 16-17 0.16150880805449092 18-19 0.18786561181773576 20-21 0.1836633547112864 22-23 0.20686846564469422 24-25 0.19661001447306786 26-27 0.2490764304050605 28-29 0.176062213180503 30-31 0.1541703075928606 32-33 0.19529680912730243 34-35 0.1519146842930753 36-37 0.15262536012725425 38-39 0.18627431592816118 40-41 0.20720835408712762 42-43 0.26959333274831326 44-45 0.1731731614198191 46-47 0.15778548466237954 48-49 0.1598866132156042 50-51 0.1648458945802007 52-53 0.1856254379926065 54-55 0.1730804645718827 56-57 0.1717981581754294 58-59 0.14655371658742117 60-61 0.16052004167650283 62-63 0.1476815282373138 64-65 0.14247505527822033 66-67 0.13102699455807706 68-69 0.1340705410653216 70-71 0.1353219485124628 72-73 0.1123949281228641 74-75 0.11414071875899931 76-77 0.13498206007002939 78-79 0.1414553899509201 80-81 0.15211552746360413 82-83 0.15325878858815284 84-85 0.14049752252224415 86-87 0.16707061893067388 88-89 0.16067453642306348 90-91 0.15049333262471742 92-93 0.15070962526990234 94-95 0.17253973295892047 96-97 0.15871245314174337 98-99 0.15393856547301965 100-101 0.13478121689950057 102-103 0.1744091193923042 104-105 0.1315986251203514 106-107 0.1376548191855284 108-109 0.14978265679053848 110-111 0.12443006887993781 112-113 0.11570111569926175 114-115 0.11344549239947643 116-117 0.11780224425248643 118-119 0.10145670006637093 120-121 0.09459713331907861 122-123 0.12222079400412068 124-125 0.11791039057507888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3236356.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.81663898333893 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9090461947682 53.738857156592616 2 9.551835332167254 12.382357247067725 3 3.1999278454275637 6.22225703789436 4 1.4143823152523243 3.66702031648516 5 0.7880412261178172 2.553909182863316 6 0.47400758535108756 1.8434147121039395 7 0.3314762169980525 1.5039622002107982 8 0.21801884330838506 1.1304998926627787 9 0.15608799178952154 0.9105389112110212 >10 0.8734029195161119 10.314618814440783 >50 0.05901232311176478 2.6420032357234464 >100 0.02431873645672163 2.621079497663903 >500 1.4748991230143185E-4 0.05617976030653398 >1k 2.949798228253268E-4 0.413302034773662 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3446 0.10647777932959168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.089894931212759E-5 0.0 0.0 0.0 0.0 8 3.089894931212759E-5 0.0 0.0 3.089894931212759E-5 0.0 9 3.089894931212759E-5 0.0 0.0 3.089894931212759E-5 0.0 10-11 3.089894931212759E-5 0.0 0.0 4.634842396819139E-5 0.0 12-13 3.089894931212759E-5 0.0 0.0 6.179789862425519E-5 0.0 14-15 3.089894931212759E-5 0.0 0.0 6.179789862425519E-5 0.0 16-17 3.089894931212759E-5 0.0 0.0 6.179789862425519E-5 0.0 18-19 4.634842396819139E-5 0.0 0.0 6.179789862425519E-5 0.0 20-21 6.179789862425519E-5 1.5449474656063796E-5 0.0 1.2359579724851037E-4 0.0 22-23 6.179789862425519E-5 3.089894931212759E-5 0.0 2.1629264518489312E-4 0.0 24-25 1.5449474656063796E-4 3.089894931212759E-5 0.0 2.4719159449702074E-4 0.0 26-27 2.1629264518489312E-4 3.089894931212759E-5 0.0 3.398884424334035E-4 0.0 28-29 2.7809054380914836E-4 3.089894931212759E-5 0.0 6.179789862425517E-4 0.0 30-31 3.0898949312127593E-4 3.089894931212759E-5 0.0 0.0016994422121670175 0.0 32-33 3.0898949312127593E-4 3.089894931212759E-5 0.0 0.004171358157137225 0.0 34-35 3.707873917455311E-4 3.089894931212759E-5 0.0 0.00769383837871977 0.0 36-37 4.3258529036978625E-4 3.089894931212759E-5 0.0 0.013672785070616457 1.5449474656063796E-5 38-39 4.3258529036978625E-4 3.089894931212759E-5 0.0 0.02405483203949133 3.089894931212759E-5 40-41 4.3258529036978625E-4 3.089894931212759E-5 0.0 0.036939693902648535 3.089894931212759E-5 42-43 4.4803476502585006E-4 3.089894931212759E-5 0.0 0.04912932940628287 3.089894931212759E-5 44-45 4.6348423968191387E-4 3.089894931212759E-5 0.0 0.06266306920499476 3.089894931212759E-5 46-47 6.02529511586488E-4 3.089894931212759E-5 0.0 0.07893136601782993 3.089894931212759E-5 48-49 6.79776884866807E-4 3.089894931212759E-5 0.0 0.09927832413986595 3.089894931212759E-5 50-51 6.79776884866807E-4 3.089894931212759E-5 0.0 0.12197360240962365 3.089894931212759E-5 52-53 6.79776884866807E-4 3.089894931212759E-5 0.0 0.14428264381297978 3.089894931212759E-5 54-55 7.106758341789346E-4 3.089894931212759E-5 0.0 0.16894000536405762 3.089894931212759E-5 56-57 7.106758341789346E-4 3.089894931212759E-5 0.0 0.19810861351470604 3.089894931212759E-5 58-59 7.106758341789346E-4 3.089894931212759E-5 0.0 0.23181936721423724 3.089894931212759E-5 60-61 7.106758341789346E-4 3.089894931212759E-5 0.0 0.27030400858249215 3.089894931212759E-5 62-63 7.106758341789346E-4 3.089894931212759E-5 0.0 0.3109206774532839 3.089894931212759E-5 64-65 7.106758341789346E-4 3.089894931212759E-5 0.0 0.35320588958693044 3.089894931212759E-5 66-67 7.415747834910621E-4 3.089894931212759E-5 0.0 0.3997706062003068 3.089894931212759E-5 68-69 7.724737328031897E-4 3.089894931212759E-5 0.0 0.4561611886949396 3.089894931212759E-5 70-71 7.724737328031897E-4 4.634842396819139E-5 0.0 0.5222849402228926 3.089894931212759E-5 72-73 7.724737328031897E-4 6.179789862425519E-5 0.0 0.5925491509586709 3.089894931212759E-5 74-75 7.724737328031897E-4 6.179789862425519E-5 0.0 0.6638948249203733 3.089894931212759E-5 76-77 7.724737328031897E-4 6.179789862425519E-5 0.0 0.746116929039945 3.089894931212759E-5 78-79 7.724737328031897E-4 6.179789862425519E-5 0.0 0.8417955255849481 3.089894931212759E-5 80-81 8.188221567713811E-4 6.179789862425519E-5 0.0 0.9534488789243211 3.089894931212759E-5 82-83 8.651705807395725E-4 6.179789862425519E-5 0.0 1.0717918547897698 3.089894931212759E-5 84-85 8.651705807395725E-4 6.179789862425519E-5 0.0 1.1897176948395047 3.089894931212759E-5 86-87 8.651705807395725E-4 6.179789862425519E-5 0.0 1.3190606966600709 3.089894931212759E-5 88-89 8.651705807395725E-4 6.179789862425519E-5 0.0 1.4713461683448916 3.089894931212759E-5 90-91 9.269684793638277E-4 6.179789862425519E-5 0.0 1.6426962917552952 3.089894931212759E-5 92-93 9.578674286759554E-4 6.179789862425519E-5 0.0 1.830592802522343 3.089894931212759E-5 94-95 9.578674286759554E-4 6.179789862425519E-5 0.0 2.020930330285049 3.089894931212759E-5 96-97 9.578674286759554E-4 6.179789862425519E-5 0.0 2.213477132923572 3.089894931212759E-5 98-99 9.578674286759554E-4 6.179789862425519E-5 0.0 2.4244706083014353 3.089894931212759E-5 100-101 9.578674286759554E-4 6.179789862425519E-5 0.0 2.6768532262828932 3.089894931212759E-5 102-103 9.733169033320191E-4 6.179789862425519E-5 0.0 2.9544184879537356 3.089894931212759E-5 104-105 0.001050564276612338 6.179789862425519E-5 0.0 3.2368194351919257 3.089894931212759E-5 106-107 0.0010969127005805294 6.179789862425519E-5 0.0 3.513735818927213 3.089894931212759E-5 108-109 0.0011432611245487207 6.179789862425519E-5 0.0 3.812822198793952 3.089894931212759E-5 110-111 0.0011432611245487207 6.179789862425519E-5 0.0 4.1626292039565485 3.089894931212759E-5 112-113 0.0011432611245487207 6.179789862425519E-5 0.0 4.552064111611949 3.089894931212759E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8485 0.0 40.863888 1 GTACATG 11325 0.0 35.24562 1 ATCAACG 9525 0.0 34.837906 3 TCAACGC 9415 0.0 34.547283 4 CAACGCA 9485 0.0 34.16777 5 TACATGG 11660 0.0 33.77262 2 AACGCAG 9520 0.0 33.011745 6 TATCAAC 10455 0.0 32.422764 2 ACATGGG 12095 0.0 31.326586 3 GGTATCA 5445 0.0 30.964 1 ACGCAGA 11100 0.0 28.093067 7 CGCAGAG 11015 0.0 26.845737 8 CGCATAG 390 0.0 25.935093 8 CATGGGA 7400 0.0 22.782236 4 GCAGAGT 12240 0.0 22.651724 9 CATGGGG 5880 0.0 21.782251 4 GAGTACA 9480 0.0 21.555315 1 AGTACAT 8720 0.0 21.520674 2 GTACCTG 3070 0.0 20.376026 1 GAGTACT 5075 0.0 20.16333 12-13 >>END_MODULE