FastQCFastQC Report
Thu 26 May 2016
SRR1364602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3236356
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80880.24991070203648794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65930.20371677281485723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44490.13746942548965566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71550.048.7971841
GTATCAA111300.043.5213241
ATCAACG135050.035.8634643
TCAACGC135000.035.7037624
CAACGCA138600.034.2531935
TATCAAC141800.033.8063242
AACGCAG142150.033.262796
GTACATG126350.033.244561
TACATGG126900.032.5199552
ACATGGG131600.030.2025073
ACGCAGA168800.027.6933527
CGCAGAG169500.027.4735878
GCAGAGT190200.023.9520479
CATGGGA82100.023.3386334
GAGTACT107900.023.03914312-13
CATGGGG63650.022.5310464
GTACTTT111350.022.37878614-15
AGTACTT115100.021.934212-13
AGAGTAC173700.021.9339710-11
GAGTACA105300.020.6524331
Produced by FastQC (version 0.11.5)