##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364602_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3236356 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.59451123423999 31.0 30.0 33.0 26.0 34.0 2 29.781486647328045 31.0 30.0 34.0 26.0 34.0 3 29.795764433826193 31.0 30.0 34.0 26.0 34.0 4 33.445825490150035 35.0 33.0 37.0 28.0 37.0 5 32.854062717451356 35.0 33.0 37.0 26.0 37.0 6 32.83722896986611 35.0 33.0 37.0 26.0 37.0 7 32.63220826139028 35.0 33.0 37.0 25.0 37.0 8 32.5651513615931 35.0 33.0 37.0 25.0 37.0 9 33.875699706707174 38.0 33.0 39.0 24.0 39.0 10-11 33.66916325027284 37.5 33.0 39.0 22.5 39.0 12-13 33.52432133547731 37.0 33.0 39.0 21.0 39.0 14-15 34.51262314776248 38.0 33.0 41.0 21.5 41.0 16-17 34.43510757160213 38.0 33.0 41.0 20.5 41.0 18-19 34.47052703719863 38.5 33.0 41.0 19.0 41.0 20-21 34.49121033038392 39.0 33.0 41.0 19.0 41.0 22-23 34.485981919170825 39.0 33.0 41.0 21.0 41.0 24-25 34.478644963656656 39.0 33.0 41.0 21.5 41.0 26-27 34.38945530096194 39.0 33.0 41.0 20.5 41.0 28-29 34.16225934353328 39.0 33.0 41.0 18.0 41.0 30-31 34.11194102255747 38.0 33.0 41.0 18.5 41.0 32-33 33.94450131567726 38.0 33.0 41.0 18.0 41.0 34-35 33.82101242879337 38.0 33.0 41.0 16.5 41.0 36-37 33.66482225688398 38.0 33.0 40.0 16.0 41.0 38-39 33.53854952916181 38.0 33.0 40.0 15.5 41.0 40-41 33.37707424646732 38.0 32.0 40.0 14.0 41.0 42-43 33.20979784053423 38.0 32.0 40.0 9.0 41.0 44-45 33.022360488153964 38.0 32.0 40.0 8.5 41.0 46-47 32.794828504651534 38.0 31.5 40.0 7.5 41.0 48-49 32.56195347483404 38.0 31.0 40.0 2.0 41.0 50-51 32.37275225593229 38.0 31.0 40.0 2.0 41.0 52-53 32.21332279267176 38.0 31.0 40.0 2.0 41.0 54-55 32.0047638455102 37.0 30.5 40.0 2.0 41.0 56-57 31.783690823877222 37.0 30.0 40.0 2.0 41.0 58-59 31.59935047318651 37.0 30.0 40.0 2.0 41.0 60-61 31.386760140108194 36.5 30.0 40.0 2.0 41.0 62-63 31.12847350538692 36.0 29.5 40.0 2.0 41.0 64-65 30.858548163428253 36.0 29.0 39.0 2.0 41.0 66-67 30.5798693036242 35.0 29.0 39.0 2.0 41.0 68-69 30.269547911292825 35.0 29.0 39.0 2.0 40.5 70-71 30.01171101077879 35.0 29.0 38.0 2.0 40.0 72-73 29.85419944530206 35.0 29.0 37.5 2.0 40.0 74-75 29.563455472760104 35.0 29.0 37.0 2.0 39.0 76-77 29.25574828603528 34.5 29.0 36.5 2.0 39.0 78-79 28.946927346682504 34.0 28.0 36.0 2.0 39.0 80-81 28.664502143769106 34.0 28.0 36.0 2.0 37.5 82-83 28.401413657829977 34.0 28.0 35.0 2.0 37.0 84-85 28.375233905046294 34.0 28.5 35.0 2.0 37.0 86-87 28.222202223735586 34.0 28.5 35.0 2.0 36.0 88-89 28.076165137580663 34.0 28.0 35.0 2.0 36.0 90-91 27.921505545125445 34.0 28.0 35.0 2.0 36.0 92-93 27.78460975862977 34.0 27.5 35.0 2.0 35.5 94-95 27.699293124736585 34.0 27.0 35.0 2.0 35.0 96-97 27.617229532226986 34.0 27.0 35.0 2.0 35.0 98-99 27.530776434978105 34.0 27.0 35.0 2.0 35.0 100-101 27.43421119308259 34.0 27.0 35.0 2.0 35.0 102-103 26.259785851741896 32.0 24.5 34.0 2.0 35.0 104-105 27.186734864767658 34.0 26.5 35.0 2.0 35.0 106-107 27.356998581120248 34.0 27.0 35.0 2.0 35.0 108-109 27.367761457639396 34.0 27.0 35.0 2.0 35.0 110-111 27.31286978317589 34.0 27.0 35.0 2.0 35.0 112-113 27.266347243628328 34.0 27.0 35.0 2.0 35.0 114-115 27.189090446168468 34.0 27.0 35.0 2.0 35.0 116-117 27.07440791433328 34.0 27.0 35.0 2.0 35.0 118-119 26.97788871187224 34.0 26.5 35.0 2.0 35.0 120-121 26.846623177425474 34.0 25.5 35.0 2.0 35.0 122-123 26.663425624375066 34.0 25.0 35.0 2.0 35.0 124-125 25.870350171612763 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7328712464842901 1101 2 0.7060679073692029 1101 3 0.5956420329821093 1101 4 0.7140822968588267 1101 5 1.0081493379574908 1101 6 0.9927347549339629 1101 7 0.9540011521164828 1101 8 1.0780175863290182 1101 9 1.198886737087122 1101 10-11 1.3066480251667585 1101 12-13 1.2752331031346245 1101 14-15 1.317035394214031 1101 16-17 1.3661584757875076 1101 18-19 1.4616592603965444 1101 20-21 1.4735659674510018 1101 22-23 1.3771541596973478 1101 24-25 1.293121799750871 1101 26-27 1.412748369320525 1101 28-29 1.5347398553123455 1101 30-31 1.3949670499717897 1101 32-33 1.5062475948319616 1101 34-35 1.3914353969923425 1101 36-37 1.4110838936914973 1101 38-39 1.519807215595641 1101 40-41 1.4497168351140672 1101 42-43 1.4554288747231539 1101 44-45 1.5237182288727098 1101 46-47 1.529119147397175 1101 48-49 1.4869831054837803 1101 50-51 1.4149277924992205 1101 52-53 1.4546728586931152 1101 54-55 1.4525139926355237 1101 56-57 1.4549562430798666 1101 58-59 1.40040859621757 1101 60-61 1.5015327108417935 1101 62-63 1.462007466347405 1101 64-65 1.4386061965559662 1101 66-67 1.2855376149990008 1101 68-69 1.3229533444931185 1101 70-71 1.307717778844836 1101 72-73 1.3341428223724492 1101 74-75 1.318606803135463 1101 76-77 1.245874398137481 1101 78-79 1.3120758907530785 1101 80-81 1.343708238980632 1101 82-83 1.2910725998882562 1101 84-85 1.3479115898354905 1101 86-87 1.3057489729227107 1101 88-89 1.3589624710677768 1101 90-91 1.3594877175621995 1101 92-93 1.282999360741524 1101 94-95 1.2551759889952443 1101 96-97 1.2910676462511539 1101 98-99 1.3431941166079042 1101 100-101 1.299427732014447 1101 102-103 1.3165133936518743 1101 104-105 1.2713073254894738 1101 106-107 1.2568871475280332 1101 108-109 1.2970771497584899 1101 110-111 1.3242560360116578 1101 112-113 1.2783176206079894 1101 114-115 1.2685193376333572 1101 116-117 1.2551033947022994 1101 118-119 1.4049475110957417 1101 120-121 1.4353901942693703 1101 122-123 1.4862664532260865 1101 124-125 1.2906485957294684 1102 1 0.35467811026424556 1102 2 -0.5482162267129524 1102 3 0.0754955732544964 1102 4 0.27496681124655 1102 5 0.547368678531619 1102 6 0.5443623615638487 1102 7 0.4122545182628201 1102 8 0.36821075315715746 1102 9 0.7104021395474192 1102 10-11 0.6899696962496336 1102 12-13 0.7275893311802264 1102 14-15 0.8844990545424167 1102 16-17 0.924878636616036 1102 18-19 0.9471158460041949 1102 20-21 0.8105585692197224 1102 22-23 0.8705198979326738 1102 24-25 0.8364671325905064 1102 26-27 0.7551434058780728 1102 28-29 0.9197993009062415 1102 30-31 0.8603812646254951 1102 32-33 0.8042784103803626 1102 34-35 0.6770537166165056 1102 36-37 0.6744076605785523 1102 38-39 0.5105032053827472 1102 40-41 0.5104693733539918 1102 42-43 0.39052162787440636 1102 44-45 0.2664274235015043 1102 46-47 0.2558437965625231 1102 48-49 0.3459272580413142 1102 50-51 0.30358062827531995 1102 52-53 0.35269444930221283 1102 54-55 0.39495961756990283 1102 56-57 0.1650757464295367 1102 58-59 0.3812393423876266 1102 60-61 0.23311839288576763 1102 62-63 0.32435747074731935 1102 64-65 0.3572354513466678 1102 66-67 0.29016679156901404 1102 68-69 0.19696575522482718 1102 70-71 0.1491572052151504 1102 72-73 0.12471654983033176 1102 74-75 0.19215071873535194 1102 76-77 0.20262626725047284 1102 78-79 0.2330904512782439 1102 80-81 0.1812357809509173 1102 82-83 0.09353714657601486 1102 84-85 0.24447247294700958 1102 86-87 0.2176913795435773 1102 88-89 0.22962063860372695 1102 90-91 0.17573037002557257 1102 92-93 0.25120547989457975 1102 94-95 0.086857831466439 1102 96-97 0.13916550202666755 1102 98-99 0.12422657861667119 1102 100-101 -0.025306987213681964 1102 102-103 0.015168826864801588 1102 104-105 0.03348169469705198 1102 106-107 0.23916370100568685 1102 108-109 0.26270844806454363 1102 110-111 0.17215908402512525 1102 112-113 0.1556879517578018 1102 114-115 0.2874739309516947 1102 116-117 0.2443336181541369 1102 118-119 0.1897842615534806 1102 120-121 0.1734309531970517 1102 122-123 0.21596465742373283 1102 124-125 0.0856712946066196 1103 1 0.28582469818454115 1103 2 -0.9200954660378287 1103 3 -0.004824644375329257 1103 4 0.2378503103960199 1103 5 0.4909561088926182 1103 6 0.5871579709527524 1103 7 0.6049453646490761 1103 8 0.6028441118106471 1103 9 0.7909168900821228 1103 10-11 0.8388782877422045 1103 12-13 0.7535429627012817 1103 14-15 0.9315250684293872 1103 16-17 1.0329643522055036 1103 18-19 1.07868137443036 1103 20-21 1.0946214266782235 1103 22-23 0.954654735996904 1103 24-25 0.795615694316453 1103 26-27 0.7797573078101578 1103 28-29 0.8422723201873055 1103 30-31 0.7830330346004573 1103 32-33 0.8025239688887922 1103 34-35 0.8820600054844903 1103 36-37 0.9911856690282121 1103 38-39 1.0095425061759045 1103 40-41 1.0923524158486302 1103 42-43 0.9850439483809197 1103 44-45 1.060876562581896 1103 46-47 1.0077455958718176 1103 48-49 0.9453784745410019 1103 50-51 1.0174587498079504 1103 52-53 0.9570285875282991 1103 54-55 0.895695763361843 1103 56-57 1.013018089209556 1103 58-59 0.935558839182125 1103 60-61 0.8108423081503844 1103 62-63 0.8319244833874997 1103 64-65 0.8409775439443337 1103 66-67 0.8582425437800687 1103 68-69 0.7437609113898063 1103 70-71 0.8312282532980575 1103 72-73 0.8315898571024327 1103 74-75 0.7410056249787225 1103 76-77 0.729318188885621 1103 78-79 0.7039735157307341 1103 80-81 0.8392204732355424 1103 82-83 0.8688363738652392 1103 84-85 0.8704170897377708 1103 86-87 0.7554996816041175 1103 88-89 0.6722130177171373 1103 90-91 0.8291810217965789 1103 92-93 0.7968769254833497 1103 94-95 0.6668775009046009 1103 96-97 0.7136864433051819 1103 98-99 0.756065036365289 1103 100-101 0.7244974934932493 1103 102-103 0.7948771664595533 1103 104-105 0.7664678708526012 1103 106-107 0.7849848202800906 1103 108-109 0.7073474968912699 1103 110-111 0.6940606328775907 1103 112-113 0.6791519394970855 1103 114-115 0.6501023072297976 1103 116-117 0.6658830778878801 1103 118-119 0.792859982886295 1103 120-121 0.7415453032066956 1103 122-123 0.7670091738945253 1103 124-125 0.6669812884515594 1104 1 0.20195221067898572 1104 2 -0.6668315299134697 1104 3 0.018365264487144373 1104 4 0.13380431396007708 1104 5 0.560747960803262 1104 6 0.4644410140412134 1104 7 0.6382372033309949 1104 8 0.5659886813195456 1104 9 0.576341692831825 1104 10-11 0.744867198388711 1104 12-13 0.7258917299350429 1104 14-15 0.7892381002617128 1104 16-17 0.8892868820262194 1104 18-19 0.86591380258416 1104 20-21 0.8739088347427924 1104 22-23 0.9090140731474676 1104 24-25 0.8568810693574349 1104 26-27 0.755703136358548 1104 28-29 0.985854635662534 1104 30-31 0.9189409327171845 1104 32-33 0.8303941872497944 1104 34-35 0.8812298612780225 1104 36-37 0.8906411187093326 1104 38-39 0.903155256085725 1104 40-41 1.0067727742498889 1104 42-43 0.9913035203664435 1104 44-45 0.9271301163358885 1104 46-47 0.869468040023996 1104 48-49 0.9420112413501016 1104 50-51 0.9630040528244024 1104 52-53 0.688600474583815 1104 54-55 0.9113867238240445 1104 56-57 0.7864382866892647 1104 58-59 0.8436660408987073 1104 60-61 0.8476661948361652 1104 62-63 0.7890608057088393 1104 64-65 0.8190768075900934 1104 66-67 0.7738604783444423 1104 68-69 0.6997686478481988 1104 70-71 0.8214398776947718 1104 72-73 0.8078833582496472 1104 74-75 0.8645877863979408 1104 76-77 0.8658937397949344 1104 78-79 0.8312805672762025 1104 80-81 0.7605015988290376 1104 82-83 0.8423055008733691 1104 84-85 0.807232942904978 1104 86-87 0.805000619671258 1104 88-89 0.7406076904603083 1104 90-91 0.8001986697497934 1104 92-93 0.7824341536071415 1104 94-95 0.6782331452321415 1104 96-97 0.6879152590129998 1104 98-99 0.8388758560872738 1104 100-101 0.8387168171739674 1104 102-103 0.8048596819396714 1104 104-105 0.775064238077924 1104 106-107 0.8083302712907816 1104 108-109 0.7144559049333985 1104 110-111 0.602850327836638 1104 112-113 0.5866759471168308 1104 114-115 0.6381547612918048 1104 116-117 0.729069610914312 1104 118-119 0.587782485177879 1104 120-121 0.6417401380100891 1104 122-123 0.6944272964742133 1104 124-125 0.6232352633477944 1105 1 0.5295506761862079 1105 2 0.33714925510482985 1105 3 0.47301690326434453 1105 4 0.42252747289158776 1105 5 0.5273543150300384 1105 6 0.4347691384486865 1105 7 0.5754992836694655 1105 8 0.46040725593851306 1105 9 0.6571162365682497 1105 10-11 0.8133931444559863 1105 12-13 0.7629926536849325 1105 14-15 0.8421511050162565 1105 16-17 0.9438483493670091 1105 18-19 0.9203929082901254 1105 20-21 0.9901678987759865 1105 22-23 0.8499087771385732 1105 24-25 0.9130095395689821 1105 26-27 0.9211833212930074 1105 28-29 1.0423621989736773 1105 30-31 0.9598475051469464 1105 32-33 1.0132920746062126 1105 34-35 1.017201971472069 1105 36-37 1.0088034170458116 1105 38-39 1.0322138060248989 1105 40-41 0.9849477199553647 1105 42-43 1.1226717012668672 1105 44-45 1.056051410549145 1105 46-47 1.0445657599103981 1105 48-49 1.0863771254309142 1105 50-51 1.0299851750375453 1105 52-53 0.9053792901382351 1105 54-55 0.8568828358342202 1105 56-57 0.9181155799473153 1105 58-59 0.8188093487084096 1105 60-61 0.9176206786564478 1105 62-63 1.0013617147000566 1105 64-65 0.9982570448973718 1105 66-67 1.0601637466746716 1105 68-69 0.9447368295685479 1105 70-71 0.9753766224013738 1105 72-73 0.9182133274921469 1105 74-75 0.9187628222953563 1105 76-77 0.9084323557792011 1105 78-79 0.9995527460366134 1105 80-81 0.7660822657778965 1105 82-83 0.7798214305113618 1105 84-85 0.6373059676247408 1105 86-87 0.6981803055778464 1105 88-89 0.7978504000310025 1105 90-91 0.7200849049232154 1105 92-93 0.5433724367011763 1105 94-95 0.6690963537897154 1105 96-97 0.7559482036669216 1105 98-99 0.807186509907055 1105 100-101 0.7776846319771309 1105 102-103 0.4130341459382265 1105 104-105 0.3385232627546415 1105 106-107 0.6757558552579326 1105 108-109 0.717471082801115 1105 110-111 0.6872230583052179 1105 112-113 0.4931107111880557 1105 114-115 0.6815620690933031 1105 116-117 0.4085140900917601 1105 118-119 0.2798823071513006 1105 120-121 0.1973663006404962 1105 122-123 0.21891709197425158 1105 124-125 0.220927559214239 1106 1 0.3424364592682849 1106 2 0.39048125967898173 1106 3 0.5718852836277009 1106 4 0.42438423265192426 1106 5 0.6558504817611279 1106 6 0.5621001444401799 1106 7 0.4861138549231683 1106 8 0.6254398709365816 1106 9 0.6984369023973969 1106 10-11 0.8234027880263994 1106 12-13 0.7033246824183479 1106 14-15 0.8044542674631145 1106 16-17 1.0436810062411226 1106 18-19 0.9630678213167982 1106 20-21 1.0172301128914398 1106 22-23 0.9101051315065618 1106 24-25 0.9359569891566721 1106 26-27 0.9492057652435335 1106 28-29 1.118802453129078 1106 30-31 1.0695900479893723 1106 32-33 1.0280927607291375 1106 34-35 0.9320404668334845 1106 36-37 0.9293261025889024 1106 38-39 1.0376229027907868 1106 40-41 1.0219259403844347 1106 42-43 1.0316209346130805 1106 44-45 0.9637724274928416 1106 46-47 0.8879055759779177 1106 48-49 0.9355553684086217 1106 50-51 1.0289806330970706 1106 52-53 1.1844417096073698 1106 54-55 1.1519634270917933 1106 56-57 0.9695421237238868 1106 58-59 0.9895190671725267 1106 60-61 1.0308860036790009 1106 62-63 0.8996698217416714 1106 64-65 0.9201524997356678 1106 66-67 0.9340248792606793 1106 68-69 0.9745799755871047 1106 70-71 1.0131962493824354 1106 72-73 1.094672365470501 1106 74-75 1.0733102187326686 1106 76-77 1.070852328237649 1106 78-79 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 123478.0 3 39474.0 4 18200.0 5 13781.0 6 14774.0 7 17372.0 8 19455.0 9 21095.0 10 22399.0 11 24991.0 12 27929.0 13 30339.0 14 32244.0 15 27415.0 16 19220.0 17 16658.0 18 15353.0 19 14302.0 20 14086.0 21 14569.0 22 17463.0 23 22965.0 24 31208.0 25 41956.0 26 50666.0 27 56307.0 28 64802.0 29 76556.0 30 88528.0 31 104022.0 32 126564.0 33 156308.0 34 202154.0 35 271805.0 36 430106.0 37 687236.0 38 279553.0 39 1023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.761569956774494 19.66656907099328 13.678749876717186 24.893111095515042 2 15.327731778454506 20.78971162671034 39.181239532645776 24.701317062189382 3 19.366627157210146 24.794027603885358 29.15284968649926 26.686495552405237 4 12.844534236805446 17.142814763907758 36.66258975287599 33.35006124641081 5 13.11950334026298 38.69546457380198 34.05606276121401 14.128969324721036 6 31.871715817777208 37.6330899106585 16.938300169388565 13.556894102175725 7 28.387822600480295 32.57564989760088 20.547584999919664 18.488942501999162 8 25.75829111506892 37.029949733589255 18.911331139095946 18.300428012245874 9 25.901890994732014 16.560471569881894 19.612685815670115 37.924951619715976 10-11 24.693470894497967 27.454768058767513 27.065087001150122 20.786674045584398 12-13 25.586497746869064 24.399372383482653 27.94225371807163 22.07187615157665 14-15 22.39060282356562 25.819800928333642 25.89693030637606 25.892665941724673 16-17 21.981379354799017 29.12662029462052 26.444710694605313 22.447289655975148 18-19 21.936579788959783 27.709255797426113 28.83481391073277 21.51935050288133 20-21 22.57304276146794 26.814100291019944 29.007681474150832 21.605175473361285 22-23 22.271529573259812 26.682583738844233 28.949406794968446 22.096479892927505 24-25 22.06078379510783 26.83221499736123 29.120776577113272 21.986224630417667 26-27 21.946890267943328 27.074895345258682 28.967038854810784 22.01117553198721 28-29 21.779629866072767 27.0612554553529 29.11118826141918 22.04792641715515 30-31 21.96243243944733 26.85148049225734 29.12318669515962 22.06290037313571 32-33 21.8530851070733 26.91814361167787 29.01519077225707 22.21358050899176 34-35 22.20378413252436 26.877404710730218 28.958665239547194 21.960145917198233 36-37 22.128160467242562 26.757234772887596 28.883519669850195 22.231085090019647 38-39 22.027876414090418 26.965034130979408 29.02134375822685 21.98574569670333 40-41 22.21059735084768 26.820349800825372 28.845312443995653 22.12374040433129 42-43 22.096315179606023 26.76868288914639 28.727029741212824 22.407972190034762 44-45 22.153866879910616 27.03395423742011 28.525894555481536 22.28628432718774 46-47 22.20995876687798 27.30254055913838 28.127558337629576 22.359942336354067 48-49 22.300800161169317 27.31449151945341 27.986684373868325 22.39802394550895 50-51 22.3487627064911 27.351237726096596 27.897750482180783 22.402249085231517 52-53 22.57608257692612 27.4421941741813 27.621083862323996 22.360639386568582 54-55 22.37919746776931 27.395502843321317 27.721424960665637 22.50387472824374 56-57 22.256826146847672 27.605578706092686 27.806085085897315 22.331510061162323 58-59 22.42043211562634 27.280496953981576 27.826697680972057 22.472373249420027 60-61 22.402278982905464 27.343144573711914 27.98752671214168 22.267049731240938 62-63 22.33990945371894 27.40291859115623 27.969497175218056 22.287674779906787 64-65 22.179169453380727 27.280489973468473 27.957613210113834 22.582727363036962 66-67 22.170073378824824 27.465179974020167 27.82013165424323 22.544614992911782 68-69 22.124697593743043 27.276005393140107 27.98867916424559 22.61061784887126 70-71 22.126382264497476 27.15171013324863 28.007518332346628 22.714389269907265 72-73 22.3351895251516 27.28815180242905 27.75456615307889 22.62209251934046 74-75 22.256062939717534 27.484507867850944 27.67713885205344 22.582290340378083 76-77 22.368254766725755 27.290155765415324 27.74990336308813 22.59168610477079 78-79 22.333447402959063 27.367455858531002 27.697459543263157 22.601637195246774 80-81 22.19659460876656 27.47506071344536 27.765793104769493 22.562551573018588 82-83 22.30683058180212 27.570739810394397 27.60528490510682 22.517144702696665 84-85 22.284921417694985 27.39202966797184 27.70120493691663 22.621843977416546 86-87 22.23892832125643 27.322412982801886 27.905679342393558 22.532979353548125 88-89 22.305275177587877 27.32133413650015 27.707292971986547 22.66609771392543 90-91 22.28863264733546 27.411662993811557 27.818926595220056 22.480777763632926 92-93 22.242117477283717 27.412808259286216 27.750976093247615 22.594098170182455 94-95 22.124168767922477 27.329968233956293 27.81957628794621 22.72628671017502 96-97 22.257282625926216 27.32892461159998 27.6425678450485 22.771224917425307 98-99 22.103632488866214 27.30497067503502 27.583975755186213 23.007421080912554 100-101 21.968642070485433 27.3855285712189 27.744622208557963 22.901207149737708 102-103 22.092135808423865 27.24365976146607 27.735324298678172 22.928880131431892 104-105 22.1381629056783 27.09237196721357 27.821853643885884 22.947611483222246 106-107 22.007554203425826 27.244449258029025 27.838415381689362 22.909581156855786 108-109 22.033131026972843 27.193596402673396 27.549231640628257 23.2240409297255 110-111 21.948743428699174 27.325024221134676 27.60765948676686 23.11857286339929 112-113 22.006806641388447 27.28240176673565 27.71285354566132 22.997938046214585 114-115 21.960153123637543 27.30563763561234 27.488390431286707 23.245818809463408 116-117 21.749596645175522 27.236133794375018 27.657356275827183 23.356913284622273 118-119 21.748059079441823 27.28639754236839 27.589677242728666 23.37586613546112 120-121 21.7373703287205 27.365603709056725 27.633560374923015 23.263465587299763 122-123 21.775764176959978 27.422014167303963 27.434466563154196 23.367755092581863 124-125 21.74023945893701 27.27695488916959 27.455863122532676 23.526942529360724 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 95.0 1 86.5 2 152.0 3 323.0 4 306.0 5 311.5 6 792.0 7 1644.0 8 2520.0 9 2873.0 10 2492.5 11 2032.5 12 2040.0 13 2229.5 14 2433.0 15 2741.5 16 3058.5 17 3369.5 18 3738.0 19 4116.0 20 4586.5 21 5232.5 22 6183.5 23 7634.5 24 9395.0 25 11743.0 26 15527.0 27 20332.0 28 24638.0 29 29969.0 30 37537.5 31 44057.5 32 50073.5 33 57839.5 34 66669.5 35 74363.5 36 82201.5 37 89785.5 38 95077.0 39 99948.0 40 103547.5 41 108241.5 42 113692.0 43 118635.5 44 124449.0 45 127156.5 46 126374.5 47 127006.5 48 129599.0 49 126876.5 50 120668.5 51 114989.0 52 109120.0 53 104374.0 54 100677.5 55 94040.5 56 83985.0 57 75717.0 58 69909.5 59 62679.5 60 52870.0 61 42316.0 62 32274.5 63 24698.0 64 18615.5 65 13632.0 66 10035.5 67 7627.5 68 5756.5 69 4131.5 70 3107.0 71 2460.5 72 1880.0 73 1614.5 74 1264.0 75 805.0 76 536.5 77 306.0 78 187.0 79 134.0 80 94.0 81 61.5 82 36.5 83 21.5 84 15.0 85 11.5 86 9.0 87 7.5 88 4.0 89 1.0 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05941867952722135 2 0.07700018168582196 3 0.0 4 0.007662939429407642 5 0.030342768224509292 6 3.0898949312127593E-4 7 0.0 8 0.0 9 5.25282138306169E-4 10-11 0.05920238688203646 12-13 0.009377831116230723 14-15 0.007261253088349984 16-17 0.05316164229151552 18-19 1.8539369587276556E-4 20-21 0.009995810102473275 22-23 0.012900311337813268 24-25 0.0 26-27 0.0 28-29 0.05218832538818351 30-31 0.0 32-33 0.0035533791708946727 34-35 0.0 36-37 2.1629264518489312E-4 38-39 0.0 40-41 0.0 42-43 0.003275288627085525 44-45 0.0 46-47 4.634842396819139E-5 48-49 2.471915944970207E-4 50-51 5.561810876182966E-4 52-53 1.2359579724851034E-4 54-55 0.0 56-57 0.0015449474656063797 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.407316129622329E-4 66-67 0.0 68-69 6.952263595228707E-4 70-71 0.0 72-73 0.0021783759265049953 74-75 0.003599727594862864 76-77 4.6348423968191387E-4 78-79 8.806200553956364E-4 80-81 0.001436801143013933 82-83 2.0084317052882934E-4 84-85 1.2359579724851034E-4 86-87 4.016863410576587E-4 88-89 1.5449474656063793E-5 90-91 0.0 92-93 0.00744664678422275 94-95 0.006056194065177008 96-97 0.00602529511586488 98-99 0.0010814632259244657 100-101 4.1713581571372244E-4 102-103 9.269684793638278E-5 104-105 5.25282138306169E-4 106-107 0.004464898175602436 108-109 0.005407316129622328 110-111 9.115190047077639E-4 112-113 0.0032443896777733967 114-115 5.25282138306169E-4 116-117 0.0019002853826958468 118-119 0.0021474769771928676 120-121 0.011896095485169123 122-123 9.578674286759552E-4 124-125 0.0018539369587276555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3236356.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.147525296830935 #Duplication Level Percentage of deduplicated Percentage of total 1 75.80629085915736 40.28916761095989 2 12.69218343647254 13.491162805238858 3 4.641431820179947 7.4004184522958925 4 2.208348823122198 4.6947309976445535 5 1.2330902706971185 3.27678481775756 6 0.7693243106855696 2.4532609958177 7 0.4984650707668118 1.8544529470716027 8 0.34847700862078596 1.4816552504829752 9 0.2561526548683633 1.2252491734020068 >10 1.375170068496582 13.466310830891898 >50 0.1147947808775145 4.18451833923708 >100 0.05445567989161445 4.928869466387237 >500 0.0014053286431786052 0.4613336249611124 >1k 2.9277680038438005E-4 0.3352013269232119 >5k 1.1711072015375202E-4 0.45688336092838866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8088 0.24991070203648794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6593 0.20371677281485723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4449 0.13746942548965566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.089894931212759E-5 0.0 0.0 0.0 0.0 6 3.089894931212759E-5 0.0 0.0 0.0 0.0 7 3.089894931212759E-5 0.0 0.0 0.0 0.0 8 3.089894931212759E-5 0.0 0.0 0.0 0.0 9 3.089894931212759E-5 0.0 0.0 0.0 0.0 10-11 3.089894931212759E-5 0.0 0.0 0.0 0.0 12-13 3.089894931212759E-5 0.0 0.0 0.0 0.0 14-15 3.089894931212759E-5 0.0 0.0 0.0 0.0 16-17 3.089894931212759E-5 0.0 0.0 1.5449474656063796E-5 0.0 18-19 4.634842396819139E-5 0.0 0.0 4.634842396819139E-5 0.0 20-21 6.179789862425519E-5 0.0 0.0 1.0814632259244656E-4 0.0 22-23 6.179789862425519E-5 0.0 0.0 1.8539369587276556E-4 0.0 24-25 9.269684793638277E-5 0.0 0.0 2.4719159449702074E-4 0.0 26-27 9.269684793638277E-5 0.0 0.0 3.398884424334035E-4 0.0 28-29 1.6994422121670175E-4 0.0 0.0 5.870800369304242E-4 0.0 30-31 1.8539369587276553E-4 0.0 0.0 0.0016376443135427622 0.0 32-33 2.008431705288293E-4 0.0 0.0 0.004047762359888714 0.0 34-35 2.4719159449702074E-4 0.0 0.0 0.007539343632159132 0.0 36-37 2.4719159449702074E-4 0.0 0.0 0.01371913349458465 0.0 38-39 2.4719159449702074E-4 0.0 0.0 0.02498180051885516 0.0 40-41 2.4719159449702074E-4 0.0 0.0 0.039056271930529275 0.0 42-43 2.626410691530845E-4 3.089894931212759E-5 0.0 0.05192568431903041 0.0 44-45 3.7078739174553106E-4 3.089894931212759E-5 0.0 0.06559846938964688 0.0 46-47 4.3258529036978625E-4 3.089894931212759E-5 0.0 0.08229935149285184 0.0 48-49 4.6348423968191387E-4 3.089894931212759E-5 0.0 0.10286260226007274 0.0 50-51 4.6348423968191387E-4 3.089894931212759E-5 0.0 0.125928667921576 0.0 52-53 4.6348423968191387E-4 6.179789862425519E-5 0.0 0.14880933988720646 0.0 54-55 4.6348423968191387E-4 6.179789862425519E-5 0.0 0.173636645659501 0.0 56-57 4.943831889940415E-4 6.179789862425519E-5 0.0 0.2033923338470799 0.0 58-59 4.943831889940415E-4 6.179789862425519E-5 0.0 0.23821544972184766 0.0 60-61 4.943831889940415E-4 6.179789862425519E-5 0.0 0.277766104841371 0.0 62-63 4.943831889940415E-4 6.179789862425519E-5 0.0 0.31909344954634167 0.0 64-65 4.943831889940415E-4 6.179789862425519E-5 0.0 0.3622283827860717 0.0 66-67 5.561810876182966E-4 9.269684793638278E-5 0.0 0.41044619318764686 0.0 68-69 5.561810876182966E-4 1.2359579724851037E-4 0.0 0.46788733995889203 0.0 70-71 5.716305622743604E-4 1.2359579724851037E-4 0.0 0.535185251560706 0.0 72-73 6.025295115864881E-4 1.2359579724851037E-4 0.0 0.6071180055593389 0.0 74-75 6.179789862425519E-4 1.2359579724851037E-4 0.0 0.6794833448483417 0.0 76-77 6.179789862425519E-4 1.2359579724851037E-4 0.0 0.7628796090417742 0.0 78-79 6.179789862425519E-4 1.2359579724851037E-4 0.0 0.8610455710064036 3.089894931212759E-5 80-81 6.79776884866807E-4 1.2359579724851037E-4 0.0 0.9741048265394784 3.089894931212759E-5 82-83 7.106758341789346E-4 1.2359579724851037E-4 0.0 1.093853704598629 3.089894931212759E-5 84-85 7.57024258147126E-4 1.2359579724851037E-4 0.0 1.2132008963167216 3.089894931212759E-5 86-87 8.188221567713811E-4 1.2359579724851037E-4 0.0 1.3450467130315702 3.089894931212759E-5 88-89 8.497211060835087E-4 1.2359579724851037E-4 0.0 1.4996650553894564 3.089894931212759E-5 90-91 9.578674286759554E-4 1.2359579724851037E-4 0.0 1.6744295126988502 3.089894931212759E-5 92-93 9.578674286759554E-4 1.2359579724851037E-4 0.0 1.865477716295735 3.089894931212759E-5 94-95 9.578674286759554E-4 1.2359579724851037E-4 0.0 2.059384072703992 3.089894931212759E-5 96-97 9.578674286759554E-4 1.2359579724851037E-4 0.0 2.2555924008360018 3.089894931212759E-5 98-99 0.0010042158526441468 1.2359579724851037E-4 0.0 2.4704791438271934 3.089894931212759E-5 100-101 0.0010351148019562741 1.2359579724851037E-4 0.0 2.7280991337170573 3.089894931212759E-5 102-103 0.001050564276612338 1.2359579724851037E-4 0.0 3.0102992377847184 3.089894931212759E-5 104-105 0.0011741600738608485 1.2359579724851037E-4 0.0 3.297242330571791 3.089894931212759E-5 106-107 0.0011896095485169123 1.2359579724851037E-4 0.0 3.5780674313950627 3.089894931212759E-5 108-109 0.001205059023172976 1.2359579724851037E-4 0.0 3.881912249455869 3.089894931212759E-5 110-111 0.001205059023172976 1.2359579724851037E-4 0.0 4.239490340370466 3.089894931212759E-5 112-113 0.001205059023172976 1.2359579724851037E-4 0.0 4.637947741225007 3.089894931212759E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7155 0.0 48.797184 1 GTATCAA 11130 0.0 43.521324 1 ATCAACG 13505 0.0 35.863464 3 TCAACGC 13500 0.0 35.703762 4 CAACGCA 13860 0.0 34.253193 5 TATCAAC 14180 0.0 33.806324 2 AACGCAG 14215 0.0 33.26279 6 GTACATG 12635 0.0 33.24456 1 TACATGG 12690 0.0 32.519955 2 ACATGGG 13160 0.0 30.202507 3 ACGCAGA 16880 0.0 27.693352 7 CGCAGAG 16950 0.0 27.473587 8 GCAGAGT 19020 0.0 23.952047 9 CATGGGA 8210 0.0 23.338633 4 GAGTACT 10790 0.0 23.039143 12-13 CATGGGG 6365 0.0 22.531046 4 GTACTTT 11135 0.0 22.378786 14-15 AGTACTT 11510 0.0 21.9342 12-13 AGAGTAC 17370 0.0 21.93397 10-11 GAGTACA 10530 0.0 20.652433 1 >>END_MODULE