FastQCFastQC Report
Thu 26 May 2016
SRR1364601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2679321
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78570.29324593805669424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.12932381002500262No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107500.042.1731071
CGCATAG3300.039.6657268
GTACATG175950.033.8248331
ATCAACG129350.033.528663
TACATGG173800.033.3856962
GGTATCA69700.033.0778731
TCAACGC129400.033.0553254
CAACGCA131600.032.4586755
TATCAAC139400.032.3080142
AACGCAG132600.031.333256
ACATGGG181850.030.2370663
ACGCATA5250.028.3374317
ACGCAGA153500.026.6722627
CGCAGAG149850.025.9276458
CATGGGG54600.025.3131754
CATGGGA133450.024.9542484
GTATAGG7950.024.728981
ATGGGAT44150.023.7492285
GTTATCA20650.023.6566471
ATGGGAG48950.023.2459965
Produced by FastQC (version 0.11.5)