##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364601_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2679321 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.191790009483746 31.0 26.0 31.0 7.0 34.0 2 25.97396915113941 31.0 25.0 31.0 7.0 34.0 3 26.382791013096227 31.0 26.0 31.0 7.0 34.0 4 29.56823911729875 35.0 28.0 37.0 7.0 37.0 5 30.007491450259227 35.0 30.0 37.0 7.0 37.0 6 29.943926091722492 35.0 30.0 37.0 7.0 37.0 7 29.868295362892315 35.0 30.0 37.0 2.0 37.0 8 29.58067174481893 35.0 30.0 37.0 2.0 37.0 9 30.39365421313833 37.0 28.0 39.0 2.0 39.0 10-11 30.366556489498645 37.0 28.5 39.0 2.0 39.0 12-13 30.364438602168235 37.0 28.5 39.0 2.0 39.0 14-15 30.96916009690515 37.0 28.0 40.0 2.0 41.0 16-17 31.37598014571602 38.0 30.0 40.0 2.0 41.0 18-19 31.198651635992853 38.0 28.5 40.0 2.0 41.0 20-21 31.115909590526854 38.0 28.0 40.0 2.0 41.0 22-23 31.18966596387667 38.0 28.5 40.0 2.0 41.0 24-25 31.295969016030554 38.0 29.0 40.0 2.0 41.0 26-27 31.124174930887342 37.5 28.5 40.0 2.0 41.0 28-29 31.0306471303737 37.0 29.0 40.0 2.0 41.0 30-31 30.886965204990368 37.0 28.5 40.0 2.0 41.0 32-33 30.781147910235468 37.0 28.5 40.0 2.0 41.0 34-35 30.817371453439137 37.0 29.0 40.0 2.0 41.0 36-37 30.73995613067639 37.0 29.0 40.0 2.0 41.0 38-39 30.434774332750724 37.0 27.5 40.0 2.0 41.0 40-41 30.291296563569652 37.0 27.0 40.0 2.0 41.0 42-43 29.762442798007406 36.0 25.5 40.0 2.0 41.0 44-45 29.613463821617493 36.0 26.0 40.0 2.0 41.0 46-47 29.545485031468793 36.0 26.0 40.0 2.0 41.0 48-49 29.461223571195838 36.0 26.0 40.0 2.0 41.0 50-51 29.225227212416875 35.0 26.0 40.0 2.0 41.0 52-53 29.130401321827435 35.0 25.5 40.0 2.0 41.0 54-55 28.947268169808694 35.0 25.5 40.0 2.0 41.0 56-57 28.735300286901044 35.0 24.5 39.0 2.0 41.0 58-59 28.52540792984491 35.0 24.5 39.0 2.0 41.0 60-61 28.2213889265228 34.0 23.5 39.0 2.0 40.0 62-63 28.038631615995246 34.0 23.5 39.0 2.0 40.0 64-65 27.806850690902657 34.0 23.0 38.0 2.0 40.0 66-67 27.532455797569607 34.0 22.0 38.0 2.0 40.0 68-69 27.285784719337475 34.0 22.0 37.5 2.0 40.0 70-71 26.847200092859346 33.0 21.0 37.0 2.0 39.5 72-73 26.61525923918784 33.0 20.5 36.5 2.0 39.0 74-75 26.583205968974976 33.0 21.0 36.0 2.0 39.0 76-77 26.339739993826797 33.0 20.0 36.0 2.0 39.0 78-79 26.05013397050969 33.0 20.0 35.5 2.0 37.5 80-81 25.646903637152846 32.5 18.0 35.0 2.0 37.0 82-83 25.762205797662915 33.0 20.0 35.0 2.0 37.0 84-85 25.597949256546713 33.0 19.0 35.0 2.0 36.0 86-87 25.473321225788176 33.0 18.5 35.0 2.0 36.0 88-89 25.310645868860057 33.0 18.0 35.0 2.0 36.0 90-91 25.179446956896918 33.0 17.5 35.0 2.0 35.5 92-93 25.0553862340496 33.0 16.5 35.0 2.0 35.0 94-95 24.95077614813604 33.0 14.0 35.0 2.0 35.0 96-97 24.838569174802124 33.0 10.5 35.0 2.0 35.0 98-99 24.74990081442276 33.0 7.5 35.0 2.0 35.0 100-101 24.641544070307365 32.5 7.0 35.0 2.0 35.0 102-103 23.53599363420807 30.5 6.0 34.0 2.0 35.0 104-105 24.347768520457237 32.0 2.0 34.5 2.0 35.0 106-107 24.492072245169577 32.5 2.0 35.0 2.0 35.0 108-109 24.44423251264033 33.0 2.0 35.0 2.0 35.0 110-111 24.37607158679382 33.0 2.0 35.0 2.0 35.0 112-113 24.284433257530544 32.5 2.0 35.0 2.0 35.0 114-115 24.174376269211493 32.0 2.0 35.0 2.0 35.0 116-117 24.061748107076383 32.0 2.0 35.0 2.0 35.0 118-119 23.934689049949597 32.0 2.0 35.0 2.0 35.0 120-121 23.742942708245856 32.0 2.0 35.0 2.0 35.0 122-123 23.526874532764083 32.0 2.0 35.0 2.0 35.0 124-125 22.60091735928618 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.592836557717522 1101 2 1.8403867138966596 1101 3 1.3240660719164765 1101 4 1.5871598553512598 1101 5 1.4251367847988874 1101 6 1.216861153957968 1101 7 1.3941900461964423 1101 8 1.5608228905993435 1101 9 1.8701960745714423 1101 10-11 1.8439347901674594 1101 12-13 1.8599724093184626 1101 14-15 2.130925981108682 1101 16-17 1.7814906816439944 1101 18-19 1.8016789255606653 1101 20-21 1.9397200496816325 1101 22-23 1.7050982358879843 1101 24-25 1.754878950396023 1101 26-27 1.8734683042761304 1101 28-29 1.8185718916915832 1101 30-31 1.914924553034723 1101 32-33 1.9730480815474536 1101 34-35 1.8027030503227905 1101 36-37 1.672438399980745 1101 38-39 1.5272044925489787 1101 40-41 1.6598323818148195 1101 42-43 1.7554656265598538 1101 44-45 1.6429170698952085 1101 46-47 1.3648677501438797 1101 48-49 1.2107231007184254 1101 50-51 1.148251522543518 1101 52-53 1.0642468123585047 1101 54-55 1.0504188260304694 1101 56-57 1.1869193107127955 1101 58-59 1.066417311989003 1101 60-61 1.1969170346696743 1101 62-63 1.0303936645420322 1101 64-65 0.9987761620318238 1101 66-67 1.1063961724719107 1101 68-69 0.9339851004046373 1101 70-71 0.8728259165805845 1101 72-73 0.7625071083406958 1101 74-75 0.8902484590280046 1101 76-77 1.0283075438117173 1101 78-79 0.9916374875192417 1101 80-81 1.0333974109649802 1101 82-83 1.0768357964163329 1101 84-85 1.1471331605333859 1101 86-87 1.0704448593942928 1101 88-89 1.0099388831668321 1101 90-91 0.9839788133460132 1101 92-93 0.9787810059323121 1101 94-95 0.980624344155089 1101 96-97 0.9251556360970952 1101 98-99 0.9476989470360309 1101 100-101 0.9019858307786173 1101 102-103 0.8481793728786187 1101 104-105 0.9323718984516844 1101 106-107 0.9888511587599425 1101 108-109 1.0077140112827507 1101 110-111 1.0210616397462928 1101 112-113 0.9058822705369174 1101 114-115 0.8787644123704688 1101 116-117 0.782417713365529 1101 118-119 0.7713428969749252 1101 120-121 0.8934370362008117 1101 122-123 0.8190164732830247 1101 124-125 0.7269233447341854 1102 1 0.9271896725678204 1102 2 1.1324324436666338 1102 3 1.0566032681902158 1102 4 0.800499181325133 1102 5 0.8135658851705827 1102 6 0.39632430997161094 1102 7 0.6297909394088173 1102 8 0.5501206251559196 1102 9 1.094873242610987 1102 10-11 1.2425137227674021 1102 12-13 1.3497489705327474 1102 14-15 1.44330817861389 1102 16-17 1.2333945568564317 1102 18-19 1.0205482801937364 1102 20-21 1.361026703048772 1102 22-23 1.30623972638109 1102 24-25 0.9641998063724451 1102 26-27 1.261153658388963 1102 28-29 0.8817548645362727 1102 30-31 0.9296676904713586 1102 32-33 1.1880055356771209 1102 34-35 1.0795881974667765 1102 36-37 0.9386780547391034 1102 38-39 1.121090744687411 1102 40-41 1.1413991876384202 1102 42-43 1.323979314307266 1102 44-45 0.8749611662396681 1102 46-47 0.6210048088821694 1102 48-49 0.5835151600174058 1102 50-51 0.718404588339844 1102 52-53 0.7431671360123033 1102 54-55 0.6551886717664885 1102 56-57 0.7659056754500568 1102 58-59 0.7536188854027763 1102 60-61 0.5986384552275901 1102 62-63 0.5217689601843745 1102 64-65 0.37930683074803895 1102 66-67 0.3043645930825676 1102 68-69 0.25687127368804497 1102 70-71 0.4219515730870711 1102 72-73 0.07811493721095175 1102 74-75 0.29222923069099593 1102 76-77 0.1471740053870434 1102 78-79 0.16197951094942198 1102 80-81 0.2903995707733955 1102 82-83 0.22081931161973145 1102 84-85 0.16347541643227714 1102 86-87 0.29187617026691015 1102 88-89 0.2078952707054853 1102 90-91 0.33448856755899925 1102 92-93 0.4875376260420836 1102 94-95 0.4142663044500523 1102 96-97 0.17787997480298046 1102 98-99 0.3450586350159135 1102 100-101 0.31670034486524656 1102 102-103 0.204565682622178 1102 104-105 0.3357204079636986 1102 106-107 0.3217060281345461 1102 108-109 0.40321772591768124 1102 110-111 0.3556421668562919 1102 112-113 0.18457263450406103 1102 114-115 0.34007590968914414 1102 116-117 0.4342323902314611 1102 118-119 0.4013571676862746 1102 120-121 0.44252780959963545 1102 122-123 0.2120825307519496 1102 124-125 0.1480128267539591 1103 1 1.0557035901825458 1103 2 1.3379665210242067 1103 3 0.8947938366140882 1103 4 0.6864326930663118 1103 5 0.8979360960404108 1103 6 0.7053781645855324 1103 7 0.8070375002499262 1103 8 0.9747152796173637 1103 9 1.4723208342338872 1103 10-11 1.4487311244864003 1103 12-13 1.3380529970724133 1103 14-15 1.708189672191466 1103 16-17 1.2678757789587465 1103 18-19 1.2282694204928717 1103 20-21 1.4718181023826986 1103 22-23 1.287681983798361 1103 24-25 1.2841145389280904 1103 26-27 1.5847230043499252 1103 28-29 1.4226460838311041 1103 30-31 1.5830831532285075 1103 32-33 1.6232114859822104 1103 34-35 1.4577004398679207 1103 36-37 1.2701322890062876 1103 38-39 1.273714640692294 1103 40-41 1.4659112275921586 1103 42-43 1.6018647048684826 1103 44-45 1.4427802720197214 1103 46-47 1.1636674429134217 1103 48-49 1.0526539086040074 1103 50-51 1.1191050408844738 1103 52-53 0.8122011193580434 1103 54-55 0.9859235223391885 1103 56-57 0.8550514298499401 1103 58-59 0.8627348135828861 1103 60-61 0.985234621605354 1103 62-63 0.8392820123751044 1103 64-65 0.7932532929411167 1103 66-67 0.7582272559612875 1103 68-69 0.7327082365649602 1103 70-71 0.8329316400028901 1103 72-73 0.727257433392527 1103 74-75 0.7447506151633299 1103 76-77 0.6766487754444022 1103 78-79 0.6473158543785118 1103 80-81 0.8258713942681126 1103 82-83 0.7358040894298483 1103 84-85 0.739485657282863 1103 86-87 0.7601300485769684 1103 88-89 0.6363829165204109 1103 90-91 0.731167052242796 1103 92-93 0.7425657136868722 1103 94-95 0.6692850553032414 1103 96-97 0.7334178512688574 1103 98-99 0.7379515336454006 1103 100-101 0.7042451292690721 1103 102-103 0.6314926676240731 1103 104-105 0.685933286812066 1103 106-107 0.7596714189018101 1103 108-109 0.7709422403401263 1103 110-111 0.6994333936205628 1103 112-113 0.7244944372646636 1103 114-115 0.7634178242851881 1103 116-117 0.7180816725219508 1103 118-119 0.7437677083444605 1103 120-121 0.8763916444066773 1103 122-123 0.7697107538768009 1103 124-125 0.5734702651504548 1104 1 0.9071370072679734 1104 2 1.2589853652453122 1104 3 0.7475450928954963 1104 4 0.8293309342723418 1104 5 0.5123343372464397 1104 6 0.48779022488703916 1104 7 0.445487248993647 1104 8 0.6417517117781664 1104 9 0.9235067638821306 1104 10-11 1.0340426822753486 1104 12-13 1.003983273454324 1104 14-15 1.008322209879907 1104 16-17 0.8019203769486936 1104 18-19 0.7562135159702592 1104 20-21 1.1245429767545616 1104 22-23 0.9507517074164547 1104 24-25 0.9228105188275926 1104 26-27 1.1803555420383702 1104 28-29 1.2244350285547227 1104 30-31 1.3289983542335335 1104 32-33 1.2960751794495522 1104 34-35 1.1770942840890228 1104 36-37 1.0777434699108106 1104 38-39 1.1938132587827468 1104 40-41 1.147923790406228 1104 42-43 1.3061184737127007 1104 44-45 1.0824360509141933 1104 46-47 0.8806686991948283 1104 48-49 0.753530793026119 1104 50-51 0.8800013976683942 1104 52-53 0.7178242349359323 1104 54-55 0.7818066881683343 1104 56-57 0.7538428871363685 1104 58-59 0.8032649643366518 1104 60-61 0.733708867836512 1104 62-63 0.6809509822243527 1104 64-65 0.6478186195742808 1104 66-67 0.5395331604838987 1104 68-69 0.4954400204845584 1104 70-71 0.5814184490481153 1104 72-73 0.4992448705784547 1104 74-75 0.37997423325377966 1104 76-77 0.41217829805746575 1104 78-79 0.39705831669007097 1104 80-81 0.6019618465294201 1104 82-83 0.5211772050077386 1104 84-85 0.5261357829110054 1104 86-87 0.5363593199337515 1104 88-89 0.6013866853646377 1104 90-91 0.539137529629734 1104 92-93 0.5330669699682744 1104 94-95 0.526306412087159 1104 96-97 0.5562966201130806 1104 98-99 0.583770000982085 1104 100-101 0.5176521644449501 1104 102-103 0.4358079942572388 1104 104-105 0.528399995354782 1104 106-107 0.5549496852334705 1104 108-109 0.5646576926014326 1104 110-111 0.5175791222637756 1104 112-113 0.4940729548526015 1104 114-115 0.5951986031796572 1104 116-117 0.47101948659230075 1104 118-119 0.4941866781937101 1104 120-121 0.5890895087282857 1104 122-123 0.5953816081481591 1104 124-125 0.3535173757032162 1105 1 0.7511048314544695 1105 2 1.017961688703302 1105 3 0.39373377672259835 1105 4 0.6921858395661715 1105 5 0.5045391696893731 1105 6 0.4399271845762094 1105 7 0.4007754957153544 1105 8 0.4803504247456267 1105 9 0.6979234710215181 1105 10-11 0.6643452563404253 1105 12-13 0.8889334312979358 1105 14-15 1.0672615615069105 1105 16-17 0.7048793983182904 1105 18-19 0.8086135281120761 1105 20-21 0.5940877800571336 1105 22-23 0.7605873679312687 1105 24-25 0.5166689452481847 1105 26-27 0.8921921738984935 1105 28-29 0.9390435763934804 1105 30-31 0.8936974193136678 1105 32-33 1.039806420343183 1105 34-35 0.7515615618937801 1105 36-37 0.49588959667137544 1105 38-39 0.7083241257084403 1105 40-41 0.7940794484926812 1105 42-43 0.8956612129064858 1105 44-45 0.6081516895737344 1105 46-47 0.06980748015646299 1105 48-49 0.23201330881047966 1105 50-51 0.4383836827582428 1105 52-53 0.21988640103597845 1105 54-55 0.2572021471289965 1105 56-57 0.33352258601932405 1105 58-59 0.35402479920795216 1105 60-61 0.32282306161989993 1105 62-63 0.3063688428362994 1105 64-65 -0.004099058910419728 1105 66-67 0.08766945237317003 1105 68-69 -0.01231428986026728 1105 70-71 0.29322448353087793 1105 72-73 0.1660149045755439 1105 74-75 0.24193902198617678 1105 76-77 0.2796999711997721 1105 78-79 0.34539549493193533 1105 80-81 0.4222765624109144 1105 82-83 0.3302547912146352 1105 84-85 0.2711499281271301 1105 86-87 0.3091533340182586 1105 88-89 0.32531055617570814 1105 90-91 0.16159084434561422 1105 92-93 0.2517047795845926 1105 94-95 0.10295174962965348 1105 96-97 0.08692781243944836 1105 98-99 0.18073679311418545 1105 100-101 0.20693676862173405 1105 102-103 0.12254488109550721 1105 104-105 0.16981652036692196 1105 106-107 0.18482444239714013 1105 108-109 0.16092438759900674 1105 110-111 0.21114347389077892 1105 112-113 0.10519309812603339 1105 114-115 0.19524140308252314 1105 116-117 0.26053763860784684 1105 118-119 0.21854030859681828 1105 120-121 0.1103185640949711 1105 122-123 0.1055328951807013 1105 124-125 0.1317996122258016 1106 1 0.3960410876577427 1106 2 -1.4489467297607774 1106 3 -0.021520071538120078 1106 4 0.37271918031375506 1106 5 0.30084864906130093 1106 6 0.33498876325488425 1106 7 0.5936985766428542 1106 8 0.5281649273689091 1106 9 0.89884902941197 1106 10-11 1.1395445093155416 1106 12-13 1.1592787972058467 1106 14-15 1.1421880746789341 1106 16-17 0.8429646826734185 1106 18-19 0.898391804642614 1106 20-21 1.1170974834537049 1106 22-23 0.8208715308025738 1106 24-25 0.9662764140772886 1106 26-27 0.9569674482503103 1106 28-29 0.9520245535242751 1106 30-31 1.1447165332834714 1106 32-33 1.3084958554544173 1106 34-35 1.0874132548563793 1106 36-37 0.8002247427488136 1106 38-39 1.1026343610969995 1106 40-41 1.1090145334025756 1106 42-43 1.2922956966116033 1106 44-45 1.138735076492754 1106 46-47 0.8876397710584101 1106 48-49 0.7515387102002968 1106 50-51 0.8104044122847149 1106 52-53 0.7400215552769787 1106 54-55 0.7492497454155931 1106 56-57 0.8515280272094508 1106 58-59 0.7429427962489576 1106 60-61 0.8440302746574595 1106 62-63 0.7216752514082714 1106 64-65 0.6277558911942585 1106 66-67 0.6370086476946213 1106 68-69 0.5519639242604413 1106 70-71 0.565646676819128 1106 72-73 0.4627168559621353 1106 74-75 0.49181893483721595 1106 76-77 0.46863734190643314 1106 78-79 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2216 74-75 -2.0156513836147703 2216 76-77 -1.9260043511125637 2216 78-79 -1.9639045590389586 2216 80-81 -1.9065989430623276 2216 82-83 -1.9505306236149629 2216 84-85 -1.829917211705414 2216 86-87 -1.9975037913269063 2216 88-89 -1.9392652886147275 2216 90-91 -1.8273843972433035 2216 92-93 -1.8668510425934102 2216 94-95 -2.106782304826254 2216 96-97 -2.072210165440982 2216 98-99 -2.1263271838541655 2216 100-101 -2.1525824699202367 2216 102-103 -1.932976774697451 2216 104-105 -1.969323030665855 2216 106-107 -1.9718893120747403 2216 108-109 -2.113849765900138 2216 110-111 -2.0183477507734615 2216 112-113 -2.292428783595131 2216 114-115 -2.1625932878028458 2216 116-117 -1.9246717987374389 2216 118-119 -1.9494571643365752 2216 120-121 -2.1475505204327696 2216 122-123 -2.07763476690792 2216 124-125 -1.9490950626099242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 266103.0 3 35253.0 4 22304.0 5 17919.0 6 16824.0 7 18051.0 8 19328.0 9 20448.0 10 22504.0 11 25123.0 12 26700.0 13 27691.0 14 27356.0 15 22112.0 16 17348.0 17 15161.0 18 13456.0 19 12647.0 20 12188.0 21 13184.0 22 16607.0 23 24308.0 24 36770.0 25 46649.0 26 50053.0 27 52238.0 28 59387.0 29 67384.0 30 78079.0 31 90962.0 32 109098.0 33 137177.0 34 178594.0 35 242937.0 36 347614.0 37 391156.0 38 98513.0 39 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.959637156318124 19.508308809118073 13.656908348007965 22.875145686555843 2 13.076597830520143 21.642713084605226 41.785761057874 23.494928027000633 3 18.7004616713912 28.11347412588669 27.982332795739985 25.203731406982126 4 11.593330578363908 18.301044792209424 36.92856843403944 33.17705619538722 5 12.732325025125327 40.76559979510315 32.69445355596395 13.807621623807576 6 29.55941689565964 39.230995142044236 17.568852216757982 13.640735745538143 7 27.72150629668646 35.45376394608313 19.525339374640875 17.299390382589543 8 25.468245618431297 37.31453531491455 19.35953790864316 17.857681158010994 9 26.182362053708673 18.9828324029325 19.376136508988473 35.45866903437035 10-11 23.449613634571158 30.066866842321865 26.449071268937473 20.034448254169508 12-13 24.40161959728417 27.4848665163273 26.888070453491352 21.225443432897176 14-15 21.70430697801039 28.098959229249274 25.380865553898552 24.81586823884178 16-17 21.462189602012042 31.640841641299605 25.36520626529851 21.53176249138984 18-19 21.383428489374783 29.6265355971347 28.063883682770648 20.92615223071987 20-21 22.774910809469734 28.338750887605162 27.8524610453979 21.03387725752721 22-23 22.472766128774374 28.372426847277698 27.398322610831237 21.756484413116688 24-25 22.00891259397547 28.13497463882341 27.938487693761594 21.91762507343952 26-27 21.99985819288723 28.44353223932326 27.812359631069526 21.744249936719978 28-29 21.760346677734034 28.228492482791367 28.164780353931345 21.846380485543254 30-31 21.842500478956296 28.119173671889236 28.1150055839295 21.92332026522497 32-33 21.716541822955037 28.041368581269328 28.01878078242041 22.22330881335522 34-35 22.07532675798882 27.929757078588263 27.93407460156365 22.060841561859263 36-37 22.253626636691877 27.739812951635752 27.706973512010784 22.299586899661588 38-39 21.87231525244176 27.969761994996357 27.965480421523715 22.192442331038173 40-41 22.101636214201093 27.81517390210766 28.077226243383475 22.005963640307773 42-43 21.973360429496644 27.815315694629234 28.011126642751684 22.20019723312244 44-45 22.05275477399504 28.01913392946775 27.632686581143005 22.2954247153942 46-47 22.001404854036114 28.272425795632245 27.209121756882492 22.51704759344915 48-49 22.123275649529255 28.069241499815046 27.307247800104413 22.50023505055129 50-51 22.19472497802183 28.168917197404603 27.196064323736962 22.4402935008366 52-53 22.45968608177714 28.27088829071081 26.90210252926869 22.367323098243364 54-55 22.15290326416019 28.16824840265416 27.11754648277327 22.56130185041238 56-57 22.264398412264292 28.54290122377835 26.874780460000675 22.317919903956685 58-59 22.45250121901966 27.95665766021862 26.892872608380614 22.69796851238111 60-61 22.3654190301056 28.038812673577944 27.272312651708386 22.32345564460807 62-63 22.259441955687564 28.19600086496476 27.128214204747326 22.41634297460035 64-65 22.098294589231916 28.04702032016563 27.130184254931933 22.724500835670526 66-67 22.238512676157406 28.51825220164052 26.701347519108793 22.541887603093286 68-69 22.311334558456657 28.209548412366498 26.91597440471452 22.563142624462326 70-71 22.09799495964841 28.367843259492144 27.131151921788216 22.403009859071233 72-73 22.454835754149865 28.16032018150893 26.78273034624801 22.6021137180932 74-75 22.147688247951514 28.44108842271525 26.799777818009186 22.611445511324053 76-77 22.303144533501968 28.305697594736756 26.90121491853358 22.4899429532277 78-79 22.354700529649516 28.453509757336015 26.798703095948213 22.39308661706626 80-81 22.12430301015134 28.726495137028024 26.93023176705582 22.21897008576482 82-83 22.323964511418954 28.65074161325461 26.675965958833903 22.34932791649253 84-85 22.288935069594988 28.237549897588472 26.782801590742594 22.690713442073946 86-87 22.19974663085776 28.182391386720308 26.99788971208001 22.619972270341922 88-89 22.40146457756754 28.160369299648845 26.951165669308885 22.487000453474725 90-91 22.199463739625518 28.392680453335515 26.99113671424828 22.41671909279069 92-93 22.164614726718682 28.232478266980465 27.028882412925874 22.57402459337498 94-95 22.068601172727778 28.20048388200338 26.99795746609143 22.732957479177408 96-97 22.34107119592473 28.28507753470281 26.79105714900409 22.582794120368373 98-99 22.177970286573856 28.12416941953664 26.68909459767103 23.00876569621848 100-101 21.81053155611634 28.543248044882535 27.005559300293225 22.640661098707902 102-103 22.2094268615209 28.233680010753087 26.892852916560184 22.664040211165826 104-105 21.976456192844246 28.172850921237973 27.136278339854385 22.714414546063395 106-107 21.868146838895782 28.520175986607587 26.94075916135949 22.67091801313714 108-109 21.967046253328444 28.72138872311941 26.46536219535147 22.846202828200667 110-111 21.82887289257347 28.68476852969512 26.78938328648295 22.69697529124846 112-113 21.99382280904685 28.62132728331581 26.801688479525794 22.583161428111545 114-115 21.72227649713277 28.82647928090321 26.662806702288194 22.78843751967582 116-117 21.585231693199237 28.72585742888297 26.866217103960192 22.8226937739576 118-119 21.799601683458096 28.911874006286908 26.481268829292727 22.807255480962276 120-121 21.695081978849675 29.002559685044606 26.55809128901988 22.744267047085838 122-123 21.805902108365636 28.92161277508362 26.51038375401118 22.76210136253956 124-125 21.862285469150308 28.74207046746126 26.51245930602764 22.883184757360787 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9101.0 1 6791.5 2 3579.0 3 2251.0 4 1615.5 5 1344.5 6 1337.0 7 1548.5 8 1917.0 9 2067.5 10 1948.0 11 1945.5 12 2043.0 13 2213.0 14 2439.0 15 2628.5 16 2880.0 17 3154.5 18 3523.5 19 3922.5 20 4310.5 21 5050.5 22 6087.5 23 7377.0 24 9147.5 25 11646.5 26 15513.5 27 20074.5 28 23831.0 29 27769.5 30 33434.0 31 39684.5 32 45502.0 33 51668.0 34 59277.0 35 65532.5 36 70205.5 37 76463.5 38 79950.5 39 79794.0 40 80091.0 41 83710.0 42 88337.5 43 92141.5 44 96035.5 45 97667.5 46 96963.0 47 97200.0 48 101335.5 49 102127.5 50 97842.5 51 92224.5 52 87386.5 53 84089.0 54 79834.5 55 73013.5 56 64942.0 57 58553.0 58 54819.5 59 51493.0 60 45133.5 61 37261.0 62 29435.0 63 22095.0 64 16989.0 65 13943.5 66 10762.0 67 8350.5 68 6464.0 69 4682.5 70 3674.5 71 2917.5 72 2441.0 73 2173.0 74 1760.5 75 1435.0 76 1084.5 77 723.5 78 538.5 79 411.5 80 301.0 81 220.0 82 173.0 83 129.5 84 94.5 85 72.0 86 54.5 87 42.5 88 35.5 89 25.0 90 16.5 91 12.5 92 11.0 93 9.5 94 6.5 95 4.0 96 2.5 97 2.5 98 2.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2154650375972121 2 0.25345973849344666 3 0.3044428047255256 4 0.2905586900561747 5 0.32504503939617535 6 0.2383439684905243 7 0.23024490160006958 8 0.20766455381792626 9 0.20762723092903013 10-11 0.14944084714000302 12-13 0.17562285370062042 14-15 0.19217555492604282 16-17 0.16528441347639944 18-19 0.19098122248136748 20-21 0.1862412155915622 22-23 0.20835502726250418 24-25 0.19975210137195207 26-27 0.24918626771484267 28-29 0.17881396070123737 30-31 0.15800645014165904 32-33 0.19822186292721178 34-35 0.155860384030133 36-37 0.15615896714130184 38-39 0.18924570814769862 40-41 0.20969865126276394 42-43 0.27454717071974577 44-45 0.17543623925613988 46-47 0.16086165114221104 48-49 0.16289574858704875 50-51 0.1682329216991917 52-53 0.1899548430367246 54-55 0.17646261870078278 56-57 0.17521230192276327 58-59 0.1498700603623082 60-61 0.1631010244759773 62-63 0.15181085058490565 64-65 0.1464736774727627 66-67 0.1339518482481196 68-69 0.13704964802649627 70-71 0.13861720936013266 72-73 0.11529040380006726 74-75 0.11706324102263223 76-77 0.1384492563601002 78-79 0.14453288725016525 80-81 0.15513258769665897 82-83 0.15701739358591224 84-85 0.14335721624993797 86-87 0.17047229503295797 88-89 0.16496716892078256 90-91 0.15406888536312 92-93 0.15410620825201607 94-95 0.17549222358948405 96-97 0.162634488364776 98-99 0.15802511158610708 100-101 0.13863587080458073 102-103 0.1769291548119841 104-105 0.13540744091506768 106-107 0.1409498899161392 108-109 0.15251998547393164 110-111 0.12790554024695064 112-113 0.11889206257854135 114-115 0.1168393036892556 116-117 0.12088883713448294 118-119 0.10474668768691771 120-121 0.09795392190782666 122-123 0.12547955246870385 124-125 0.121262066023444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2679321.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.17260320353135 #Duplication Level Percentage of deduplicated Percentage of total 1 78.67968874828397 41.0492418124157 2 9.80976354976462 10.236018024046695 3 4.134628100043972 6.471429337732948 4 2.122352212184993 4.4291455929785855 5 1.2735057328912605 3.3221054639779064 6 0.8525962547983946 2.6689299656648133 7 0.5959798741804188 2.1765675045033874 8 0.4008619227126445 1.6731208026473166 9 0.3281079508817915 1.5406421336351517 >10 1.6340669278093412 15.085514916871587 >50 0.1030266149737704 3.723256056725319 >100 0.061722178848230336 5.704707798813195 >500 0.003020353164602329 1.0439654276419468 >1k 6.040706329188682E-4 0.5658292617807629 >5k 7.550882911485853E-5 0.3095259005647465 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7857 0.29324593805669424 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3465 0.12932381002500262 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.7322888896104644E-5 0.0 0.0 0.0 0.0 12-13 3.7322888896104644E-5 0.0 0.0 0.0 0.0 14-15 3.7322888896104644E-5 0.0 0.0 3.7322888896104644E-5 0.0 16-17 3.7322888896104644E-5 0.0 0.0 2.0527588892857554E-4 0.0 18-19 3.7322888896104644E-5 0.0 0.0 3.1724455561688947E-4 0.0 20-21 3.7322888896104644E-5 0.0 0.0 3.7322888896104646E-4 0.0 22-23 5.598433334415696E-5 0.0 0.0 6.718120001298837E-4 0.0 24-25 7.464577779220929E-5 0.0 0.0 0.0010077180001948254 0.0 26-27 7.464577779220929E-5 0.0 0.0 0.0014929155558441858 0.0 28-29 7.464577779220929E-5 0.0 0.0 0.002873862445000058 0.0 30-31 7.464577779220929E-5 0.0 0.0 0.006139615223409214 0.0 32-33 7.464577779220929E-5 0.0 0.0 0.01237253766905869 0.0 34-35 7.464577779220929E-5 0.0 0.0 0.01951987089266273 0.0 36-37 7.464577779220929E-5 0.0 0.0 0.03176177845058505 0.0 38-39 7.464577779220929E-5 0.0 0.0 0.05292385645467639 0.0 40-41 7.464577779220929E-5 0.0 0.0 0.08248358446039128 0.0 42-43 7.464577779220929E-5 0.0 0.0 0.10788181035419048 0.0 44-45 7.464577779220929E-5 0.0 0.0 0.13729224680432095 0.0 46-47 7.464577779220929E-5 0.0 0.0 0.17377537070026322 0.0 48-49 7.464577779220929E-5 0.0 0.0 0.21492385570821862 0.0 50-51 7.464577779220929E-5 0.0 0.0 0.26269715349523254 0.0 52-53 7.464577779220929E-5 0.0 0.0 0.3091081658375387 0.0 54-55 7.464577779220929E-5 0.0 0.0 0.35645225040224743 0.0 56-57 7.464577779220929E-5 0.0 0.0 0.41157815730179403 0.0 58-59 7.464577779220929E-5 0.0 0.0 0.47512037564741216 0.0 60-61 7.464577779220929E-5 0.0 0.0 0.5514830063288423 0.0 62-63 7.464577779220929E-5 3.7322888896104644E-5 0.0 0.6280135900103049 0.0 64-65 7.464577779220929E-5 3.7322888896104644E-5 0.0 0.7049733869140726 0.0 66-67 7.464577779220929E-5 3.7322888896104644E-5 0.0 0.7929434360421912 0.0 68-69 7.464577779220929E-5 3.7322888896104644E-5 0.0 0.9008252463963817 0.0 70-71 1.1196866668831394E-4 3.7322888896104644E-5 0.0 1.0262674759761894 0.0 72-73 1.1196866668831394E-4 3.7322888896104644E-5 0.0 1.1549754583344063 0.0 74-75 1.1196866668831394E-4 3.7322888896104644E-5 0.0 1.2810894999143438 0.0 76-77 1.1196866668831394E-4 7.464577779220929E-5 0.0 1.4207144272746715 0.0 78-79 1.1196866668831394E-4 1.1196866668831394E-4 0.0 1.587099865973506 0.0 80-81 1.1196866668831394E-4 1.1196866668831394E-4 0.0 1.781832038788932 0.0 82-83 1.1196866668831394E-4 1.1196866668831394E-4 0.0 1.9921091948295855 0.0 84-85 1.1196866668831394E-4 1.1196866668831394E-4 0.0 2.2003709148698496 0.0 86-87 1.1196866668831394E-4 1.1196866668831394E-4 0.0 2.418411231800893 0.0 88-89 1.1196866668831394E-4 1.1196866668831394E-4 0.0 2.6675228537379434 0.0 90-91 1.1196866668831394E-4 1.1196866668831394E-4 0.0 2.95595413912704 0.0 92-93 1.1196866668831394E-4 1.1196866668831394E-4 0.0 3.2726761742993844 0.0 94-95 1.1196866668831394E-4 1.1196866668831394E-4 0.0 3.5848074941375074 0.0 96-97 1.1196866668831394E-4 1.1196866668831394E-4 0.0 3.8939716443083903 0.0 98-99 1.1196866668831394E-4 1.1196866668831394E-4 0.0 4.236875686041351 0.0 100-101 1.1196866668831394E-4 1.1196866668831394E-4 0.0 4.644889507453568 0.0 102-103 1.1196866668831394E-4 1.1196866668831394E-4 0.0 5.085971408427732 0.0 104-105 1.1196866668831394E-4 1.1196866668831394E-4 0.0 5.52300377595667 0.0 106-107 1.1196866668831394E-4 1.1196866668831394E-4 0.0 5.964645520264275 0.0 108-109 1.1196866668831394E-4 1.3063011113636626E-4 0.0 6.425153984908864 0.0 110-111 1.1196866668831394E-4 1.4929155558441858E-4 0.0 6.950660260566017 0.0 112-113 1.1196866668831394E-4 1.4929155558441858E-4 0.0 7.535211346456808 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10750 0.0 42.173107 1 CGCATAG 330 0.0 39.665726 8 GTACATG 17595 0.0 33.824833 1 ATCAACG 12935 0.0 33.52866 3 TACATGG 17380 0.0 33.385696 2 GGTATCA 6970 0.0 33.077873 1 TCAACGC 12940 0.0 33.055325 4 CAACGCA 13160 0.0 32.458675 5 TATCAAC 13940 0.0 32.308014 2 AACGCAG 13260 0.0 31.33325 6 ACATGGG 18185 0.0 30.237066 3 ACGCATA 525 0.0 28.337431 7 ACGCAGA 15350 0.0 26.672262 7 CGCAGAG 14985 0.0 25.927645 8 CATGGGG 5460 0.0 25.313175 4 CATGGGA 13345 0.0 24.954248 4 GTATAGG 795 0.0 24.72898 1 ATGGGAT 4415 0.0 23.749228 5 GTTATCA 2065 0.0 23.656647 1 ATGGGAG 4895 0.0 23.245996 5 >>END_MODULE