FastQCFastQC Report
Thu 26 May 2016
SRR1364601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2679321
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150940.5633516849978035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123100.45944476231104825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83930.3132510065050063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.13365326513695075No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.11995576491208033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97500.052.8647541
GTATCAA161550.048.61421
ATCAACG211250.036.7264023
TATCAAC211850.036.5180932
TCAACGC212600.036.4119574
CAACGCA215250.035.3938525
AACGCAG219700.034.776316
GTACATG181400.032.388761
TACATGG183300.031.094022
ACATGGG187150.029.0572093
ACGCAGA262200.028.7760267
CGCAGAG261700.028.7627358
TACCGTA1107.7154575E-427.0684997
TATAACG1358.197337E-526.469452
GCAGAGT280250.026.137419
CATGGGA136650.025.9082054
GAGTACT154600.025.75249712-13
AGTACTT157000.025.35882812-13
ACCGTAT1651.1032393E-525.2639318
GTACTTT163500.024.1685514-15
Produced by FastQC (version 0.11.5)