##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364601_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2679321 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.548644973857183 31.0 30.0 33.0 26.0 34.0 2 29.699186099761842 31.0 30.0 34.0 26.0 34.0 3 29.70726613197896 31.0 30.0 34.0 26.0 34.0 4 33.345155358391175 35.0 33.0 37.0 28.0 37.0 5 32.75357599929236 35.0 33.0 37.0 26.0 37.0 6 32.73075641179239 35.0 33.0 37.0 25.0 37.0 7 32.52375732508348 35.0 33.0 37.0 24.0 37.0 8 32.4473659557776 35.0 33.0 37.0 23.0 37.0 9 33.7169715760075 38.0 33.0 39.0 23.0 39.0 10-11 33.51122616513662 37.0 33.0 39.0 20.5 39.0 12-13 33.37693243922621 37.0 33.0 39.0 18.5 39.0 14-15 34.32212433672561 38.0 33.0 41.0 18.0 41.0 16-17 34.25574632528166 38.0 33.0 41.0 18.0 41.0 18-19 34.335047946849215 38.5 33.0 41.0 18.0 41.0 20-21 34.370245297222695 39.0 33.0 41.0 18.0 41.0 22-23 34.35397774286844 39.0 33.0 41.0 19.0 41.0 24-25 34.343592275804205 39.0 33.0 41.0 19.0 41.0 26-27 34.23356104027849 39.0 33.0 41.0 18.5 41.0 28-29 33.978079334279094 38.5 33.0 41.0 17.5 41.0 30-31 33.8682432974623 38.0 33.0 40.5 17.0 41.0 32-33 33.63420881633817 38.0 33.0 40.0 16.0 41.0 34-35 33.47115855099109 38.0 33.0 40.0 15.5 41.0 36-37 33.266934047842724 38.0 32.0 40.0 12.5 41.0 38-39 33.103731318494496 38.0 32.0 40.0 9.0 41.0 40-41 32.894289821936226 38.0 32.0 40.0 8.5 41.0 42-43 32.670100372445106 38.0 31.0 40.0 4.5 41.0 44-45 32.40017582812959 38.0 31.0 40.0 2.0 41.0 46-47 32.031677988565015 38.0 30.5 40.0 2.0 41.0 48-49 31.697660713292656 38.0 30.0 40.0 2.0 41.0 50-51 31.474261762588355 37.0 30.0 40.0 2.0 41.0 52-53 31.304234916234375 37.0 30.0 40.0 2.0 41.0 54-55 31.084877101325297 37.0 29.0 40.0 2.0 41.0 56-57 30.86665240932311 37.0 29.0 40.0 2.0 41.0 58-59 30.676819612133073 36.0 28.5 40.0 2.0 41.0 60-61 30.464088102918613 36.0 28.0 40.0 2.0 41.0 62-63 30.20849984007142 35.5 28.0 39.5 2.0 41.0 64-65 29.947860484055475 35.0 28.0 39.0 2.0 41.0 66-67 29.672242333038856 35.0 28.0 39.0 2.0 41.0 68-69 29.361040726363136 35.0 28.0 38.5 2.0 40.0 70-71 29.10779559448084 35.0 28.0 38.0 2.0 40.0 72-73 28.952829280254214 35.0 28.0 37.0 2.0 40.0 74-75 28.67209005565216 34.5 27.5 37.0 2.0 39.0 76-77 28.38503187934555 34.0 27.0 36.0 2.0 39.0 78-79 28.080771583546728 34.0 26.0 36.0 2.0 38.5 80-81 27.80879540002859 34.0 26.0 35.5 2.0 37.0 82-83 27.560217682017196 34.0 26.0 35.0 2.0 37.0 84-85 27.53624071173256 34.0 26.0 35.0 2.0 36.5 86-87 27.3887391618996 34.0 26.0 35.0 2.0 36.0 88-89 27.243664346302666 34.0 26.0 35.0 2.0 36.0 90-91 27.093032339163543 34.0 26.0 35.0 2.0 36.0 92-93 26.9552776617658 34.0 26.0 35.0 2.0 35.0 94-95 26.87291463023654 34.0 25.5 35.0 2.0 35.0 96-97 26.79088787793624 34.0 25.0 35.0 2.0 35.0 98-99 26.700100510539798 34.0 25.0 35.0 2.0 35.0 100-101 26.60479315468359 34.0 25.0 35.0 2.0 35.0 102-103 25.45633520582267 31.5 22.0 34.0 2.0 35.0 104-105 26.355161811518663 33.5 24.5 35.0 2.0 35.0 106-107 26.514965545375112 34.0 25.0 35.0 2.0 35.0 108-109 26.523335203210067 34.0 25.0 35.0 2.0 35.0 110-111 26.46736094704591 34.0 25.0 35.0 2.0 35.0 112-113 26.41336648352325 34.0 25.0 35.0 2.0 35.0 114-115 26.33326988442221 34.0 24.5 35.0 2.0 35.0 116-117 26.218671073753388 34.0 24.0 35.0 2.0 35.0 118-119 26.115763098187937 34.0 24.0 35.0 2.0 35.0 120-121 25.981486354191976 34.0 23.5 35.0 2.0 35.0 122-123 25.796573086987337 34.0 21.5 35.0 2.0 35.0 124-125 24.998301808555226 33.0 10.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7382733825312506 1101 2 0.8588921261670635 1101 3 0.6449100827091456 1101 4 0.6994591953926275 1101 5 0.9680508800763903 1101 6 0.9717760992527573 1101 7 0.9370834705697533 1101 8 1.0313320819855605 1101 9 1.029340792505856 1101 10-11 1.1793300892523462 1101 12-13 1.2204561167811363 1101 14-15 1.3695140891456674 1101 16-17 1.4337806249297813 1101 18-19 1.4241388720305608 1101 20-21 1.3985247854730645 1101 22-23 1.4373100071555385 1101 24-25 1.320060786008682 1101 26-27 1.4373427415224214 1101 28-29 1.5609939548006935 1101 30-31 1.5129354557110872 1101 32-33 1.6120773909735533 1101 34-35 1.5525988945231717 1101 36-37 1.576024121256225 1101 38-39 1.6573392610933837 1101 40-41 1.6832890177419841 1101 42-43 1.6632141887454353 1101 44-45 1.5890413620045862 1101 46-47 1.6689728027471986 1101 48-49 1.7720978246091157 1101 50-51 1.8011349379756432 1101 52-53 1.7061182999704627 1101 54-55 1.669021403907987 1101 56-57 1.7087464494320166 1101 58-59 1.7345647992848967 1101 60-61 1.7303408365665902 1101 62-63 1.6946540970251043 1101 64-65 1.654255661609593 1101 66-67 1.6947921742327203 1101 68-69 1.6548176441394595 1101 70-71 1.5831480871751467 1101 72-73 1.6665266009567787 1101 74-75 1.6238886166370925 1101 76-77 1.6894218727128418 1101 78-79 1.6041226033863154 1101 80-81 1.59792779614434 1101 82-83 1.6259078022468536 1101 84-85 1.654687821115516 1101 86-87 1.6726792504572003 1101 88-89 1.5630068832534114 1101 90-91 1.6300008892416677 1101 92-93 1.5218600315559456 1101 94-95 1.4368343024441614 1101 96-97 1.456740822259441 1101 98-99 1.5431985103507593 1101 100-101 1.556185440086562 1101 102-103 1.5590462847846425 1101 104-105 1.466995787731154 1101 106-107 1.4602435362082744 1101 108-109 1.4466848647420925 1101 110-111 1.4368994763855696 1101 112-113 1.4464080975981837 1101 114-115 1.4121557135102236 1101 116-117 1.4212517904535424 1101 118-119 1.4115564338603868 1101 120-121 1.4507780873171434 1101 122-123 1.5107881378643846 1101 124-125 1.3362922421324157 1102 1 0.4280020290769144 1102 2 -0.560177783933085 1102 3 0.06418929145529262 1102 4 0.31188713137258617 1102 5 0.6221210538800364 1102 6 0.6289935614420301 1102 7 0.5463710307799161 1102 8 0.6203033362566615 1102 9 0.8212203634730741 1102 10-11 0.7977718729939767 1102 12-13 0.9213856971955892 1102 14-15 0.9141377765807306 1102 16-17 1.1567180539794535 1102 18-19 1.0506287327592716 1102 20-21 0.9313177869359706 1102 22-23 0.9980528266118327 1102 24-25 1.0662707523621577 1102 26-27 1.0472245946769547 1102 28-29 1.121270748739711 1102 30-31 1.0793258823014114 1102 32-33 1.0326325533918634 1102 34-35 0.8896992621207147 1102 36-37 0.830876713563967 1102 38-39 0.7345166326710668 1102 40-41 0.7833582207071359 1102 42-43 0.6393137555036432 1102 44-45 0.48912686082150714 1102 46-47 0.5780069387803053 1102 48-49 0.720928530039 1102 50-51 0.7219600764455798 1102 52-53 0.6672224828199091 1102 54-55 0.8361689027443653 1102 56-57 0.5237976207107629 1102 58-59 0.7155693150151947 1102 60-61 0.5525331880388009 1102 62-63 0.6863473089899692 1102 64-65 0.6026387111587077 1102 66-67 0.6648715566647425 1102 68-69 0.49674487375249043 1102 70-71 0.43845020433015947 1102 72-73 0.5375174757918373 1102 74-75 0.5831661407531605 1102 76-77 0.6820637131891267 1102 78-79 0.7218412507051681 1102 80-81 0.5429774287260081 1102 82-83 0.38743964559571253 1102 84-85 0.5647560972780994 1102 86-87 0.524801180295686 1102 88-89 0.62299136315076 1102 90-91 0.6058772041108043 1102 92-93 0.6529743682699767 1102 94-95 0.5800350919160948 1102 96-97 0.46652338976881325 1102 98-99 0.4671146121884391 1102 100-101 0.331993051235024 1102 102-103 0.2856374153352803 1102 104-105 0.36439496722925924 1102 106-107 0.6254878254915148 1102 108-109 0.6669948597189261 1102 110-111 0.48517148884261374 1102 112-113 0.5093746671639323 1102 114-115 0.6040113335436992 1102 116-117 0.5563754443496158 1102 118-119 0.5581338441487134 1102 120-121 0.5161798818322119 1102 122-123 0.48828014867264 1102 124-125 0.38280723406001727 1103 1 0.42141386014408866 1103 2 -0.7873071519417607 1103 3 0.17110147925230024 1103 4 0.40796902726929574 1103 5 0.5586173487835637 1103 6 0.6915196722626646 1103 7 0.6576681622599736 1103 8 0.7445402306208493 1103 9 0.8471453497576462 1103 10-11 1.0050489683631838 1103 12-13 1.0172431237440236 1103 14-15 1.0017633725256019 1103 16-17 1.094438434456933 1103 18-19 1.183180458584708 1103 20-21 1.1736701175279407 1103 22-23 1.1447674994120192 1103 24-25 1.019792461368496 1103 26-27 1.0823329272161217 1103 28-29 1.1233321191489054 1103 30-31 0.9984988872544207 1103 32-33 0.9221871304802747 1103 34-35 1.041580013906298 1103 36-37 1.1131618578914484 1103 38-39 1.056017718314557 1103 40-41 1.040063775647397 1103 42-43 0.9896916058007506 1103 44-45 1.0419978276494888 1103 46-47 1.0756415109234396 1103 48-49 1.0050914641354147 1103 50-51 1.0462463592177151 1103 52-53 1.0513357709417122 1103 54-55 0.9790027542159727 1103 56-57 1.0619879315679661 1103 58-59 0.9917997753640933 1103 60-61 0.9851040381890854 1103 62-63 0.9450952387979008 1103 64-65 0.8996571194482357 1103 66-67 0.836995824853453 1103 68-69 0.7754472657741047 1103 70-71 0.8522148244579633 1103 72-73 0.9098819892870438 1103 74-75 0.9202218060607343 1103 76-77 0.8682003806370808 1103 78-79 0.8897766478343314 1103 80-81 0.975408355785703 1103 82-83 1.0388916776679125 1103 84-85 0.9733302339790342 1103 86-87 0.9163005788775251 1103 88-89 0.8917792415483845 1103 90-91 0.9693443347408923 1103 92-93 0.9754431318727121 1103 94-95 0.9056279459865486 1103 96-97 0.8654710367685468 1103 98-99 0.8761719439523326 1103 100-101 0.9107716829893882 1103 102-103 0.9020648240343405 1103 104-105 0.9315406649644729 1103 106-107 0.8892434307860562 1103 108-109 0.8499896002076319 1103 110-111 0.8534000612278625 1103 112-113 0.8803466464864833 1103 114-115 0.8840738707298854 1103 116-117 0.8360243294723126 1103 118-119 0.899249017658498 1103 120-121 0.9535018710710759 1103 122-123 0.9360422807064666 1103 124-125 0.933447222830111 1104 1 0.34256084506871076 1104 2 -0.5635609207859815 1104 3 0.11400348930255078 1104 4 0.26449540917884207 1104 5 0.4607781528036625 1104 6 0.45171690844356505 1104 7 0.6300387652750459 1104 8 0.6958140999675848 1104 9 0.7158350482501135 1104 10-11 0.8677826367049022 1104 12-13 0.7418191287691442 1104 14-15 0.7397162619728377 1104 16-17 0.9171105450096917 1104 18-19 0.8485341018007873 1104 20-21 0.9404238863721659 1104 22-23 0.7926092079547331 1104 24-25 0.7872246221725163 1104 26-27 0.6727619473166229 1104 28-29 0.9050400337217681 1104 30-31 0.7802080581086912 1104 32-33 0.9105872561084141 1104 34-35 0.9360215968208649 1104 36-37 0.8500161292482318 1104 38-39 0.8223701052492274 1104 40-41 0.7955166650946381 1104 42-43 0.9133341937404467 1104 44-45 0.8071774758906969 1104 46-47 0.8768286968530887 1104 48-49 0.7823609364972235 1104 50-51 0.8816992486649546 1104 52-53 0.6051793639065295 1104 54-55 0.7064474778340646 1104 56-57 0.8091173285528903 1104 58-59 0.591389599484696 1104 60-61 0.6886932341689871 1104 62-63 0.7063314197024262 1104 64-65 0.6615139033678368 1104 66-67 0.631811779627327 1104 68-69 0.6883637230605366 1104 70-71 0.7387474877745497 1104 72-73 0.6442997028548803 1104 74-75 0.7019680372165134 1104 76-77 0.6641526419436126 1104 78-79 0.5695768990906132 1104 80-81 0.5071099889515338 1104 82-83 0.5738734615875138 1104 84-85 0.6260293545820446 1104 86-87 0.5721422874202382 1104 88-89 0.5363407993373279 1104 90-91 0.5733795106202919 1104 92-93 0.6203708956918099 1104 94-95 0.5640550816649395 1104 96-97 0.5737719161655335 1104 98-99 0.6786041047563529 1104 100-101 0.6432584920346152 1104 102-103 0.6072218592102203 1104 104-105 0.649220313205678 1104 106-107 0.5918860187257522 1104 108-109 0.5806912333734608 1104 110-111 0.5273441567052508 1104 112-113 0.5231820736221628 1104 114-115 0.6218113079158201 1104 116-117 0.6550557365074887 1104 118-119 0.5398696206735707 1104 120-121 0.4138756147896707 1104 122-123 0.5728927832190287 1104 124-125 0.5020496097647822 1105 1 0.5608157017952777 1105 2 0.2820702108313071 1105 3 0.5938784285934702 1105 4 0.4582915480812204 1105 5 0.6633133640712714 1105 6 0.4997624402551253 1105 7 0.5927649673148707 1105 8 0.5592703894284838 1105 9 0.5575474086192216 1105 10-11 0.7466952156267013 1105 12-13 0.7131993497502052 1105 14-15 0.8135287316182982 1105 16-17 0.972668218637665 1105 18-19 0.8712476156424529 1105 20-21 0.8739382137155332 1105 22-23 0.7207949777458964 1105 24-25 0.8324023783648471 1105 26-27 0.7722651648979806 1105 28-29 0.9926955096809778 1105 30-31 0.8716468026448752 1105 32-33 0.8516051652876229 1105 34-35 0.7444546347033381 1105 36-37 0.8119787324536745 1105 38-39 0.8077724243361573 1105 40-41 0.833677119198569 1105 42-43 0.7995229382766311 1105 44-45 0.8480828911408125 1105 46-47 0.8753055059837394 1105 48-49 0.7888548048799287 1105 50-51 0.7756780611953111 1105 52-53 0.7301684969808448 1105 54-55 0.710225282593651 1105 56-57 0.7546958861051003 1105 58-59 0.7245411900626451 1105 60-61 0.7298737829790909 1105 62-63 0.7519113128544426 1105 64-65 0.7052052583945461 1105 66-67 0.693824467825479 1105 68-69 0.5543433855180417 1105 70-71 0.6142113051616285 1105 72-73 0.6145878888675078 1105 74-75 0.5597667259003423 1105 76-77 0.6117002378639604 1105 78-79 0.7219492886000864 1105 80-81 0.5366674804522624 1105 82-83 0.6234587578478319 1105 84-85 0.3718600369521283 1105 86-87 0.5111842981050359 1105 88-89 0.537857190789488 1105 90-91 0.503382910328888 1105 92-93 0.3870279500871483 1105 94-95 0.5682284668033581 1105 96-97 0.5395371541451333 1105 98-99 0.5560718075246882 1105 100-101 0.6081334920346144 1105 102-103 0.2897977455628151 1105 104-105 0.16907303297255538 1105 106-107 0.5145191537427536 1105 108-109 0.5108601867488893 1105 110-111 0.44462323635556444 1105 112-113 0.2801906943118162 1105 114-115 0.4007453778526795 1105 116-117 0.24497693611894888 1105 118-119 0.13698059687561326 1105 120-121 0.07708500041375643 1105 122-123 0.14594365742980742 1105 124-125 0.11711062239227132 1106 1 0.42352704482510717 1106 2 0.5431205652067099 1106 3 0.5867653650638189 1106 4 0.5183002203846883 1106 5 0.6376112831325287 1106 6 0.612026591756603 1106 7 0.7368435764808936 1106 8 0.8058305433293356 1106 9 0.7513457059845834 1106 10-11 0.8931041957791948 1106 12-13 0.7121944332758474 1106 14-15 0.8467566091104715 1106 16-17 0.9820883160206577 1106 18-19 0.9218749970504803 1106 20-21 0.9824019618898276 1106 22-23 0.7590592688560704 1106 24-25 0.8677958767401179 1106 26-27 0.8562002847100487 1106 28-29 0.9864132066815756 1106 30-31 0.8556497450758016 1106 32-33 0.9227360076309452 1106 34-35 0.849598180255704 1106 36-37 0.8343510866172963 1106 38-39 0.8226583208399703 1106 40-41 1.039106951330929 1106 42-43 0.9505782558598099 1106 44-45 0.8513744917250463 1106 46-47 0.5850945312014417 1106 48-49 0.6366960461196349 1106 50-51 0.7714771110279308 1106 52-53 0.8244697110441663 1106 54-55 0.8790315216830287 1106 56-57 0.6766614820242687 1106 58-59 0.6710008966832319 1106 60-61 0.7283999942177104 1106 62-63 0.7105713709813521 1106 64-65 0.6830114673142411 1106 66-67 0.620406024450709 1106 68-69 0.6212843990654093 1106 70-71 0.7393808617087743 1106 72-73 0.7432724495053051 1106 74-75 0.6994619470825292 1106 76-77 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-2.186182074767 2216 116-117 -2.2869674801816124 2216 118-119 -2.3772921110446887 2216 120-121 -2.380542566008895 2216 122-123 -2.378850487305872 2216 124-125 -2.516241383954373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108004.0 3 34652.0 4 16042.0 5 12753.0 6 14512.0 7 17253.0 8 18817.0 9 19898.0 10 21670.0 11 24840.0 12 28944.0 13 33673.0 14 38464.0 15 31208.0 16 18167.0 17 14970.0 18 13565.0 19 12609.0 20 12453.0 21 12831.0 22 14739.0 23 19440.0 24 26571.0 25 35426.0 26 42129.0 27 45689.0 28 52758.0 29 61463.0 30 71180.0 31 84234.0 32 101331.0 33 126806.0 34 162932.0 35 224026.0 36 347521.0 37 541001.0 38 216036.0 39 714.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90760118607482 19.75180562713333 13.805447877687902 23.535145309103946 2 15.485832719226721 21.48240169034012 38.60505107511921 24.426714515313947 3 18.967380168333694 26.206565021511047 29.954230941346704 24.87182386880855 4 12.306816014380857 17.5234974806998 36.56439789676972 33.60528860814963 5 12.593433580722396 39.548337936021824 33.26170138812405 14.596527095131728 6 30.68384126337193 37.83640241166929 17.088716257120314 14.391040067838462 7 27.411982364188535 34.5744313577955 20.09371030944034 17.91987596857562 8 26.50895506734729 35.60040025066053 19.001642580340317 18.889002101651876 9 27.026306792649446 15.962927741043584 19.710275936921025 37.30048952938594 10-11 24.57769440733813 27.895901806784153 26.412039103109674 21.11436468276805 12-13 25.816606841545266 25.32714384549647 27.71514694434631 21.141102368611957 14-15 22.725321608068334 25.774761441744754 26.83660462503476 24.663312325152148 16-17 21.312799547256418 29.452026787453057 27.10766719612472 22.127506469165805 18-19 21.103664774519196 26.887783798793645 31.08914989219275 20.9194015344944 20-21 22.22357843296443 25.929598478256892 31.072088324406604 20.77473476437207 22-23 21.88324942067417 25.987989440741536 30.824557592154616 21.304203546429683 24-25 21.42287169025287 26.051189835036563 31.260662682821504 21.265275791889067 26-27 21.316725394232343 26.504662935124234 31.06398971978348 21.114621950859938 28-29 21.13123478075133 26.138757351263525 31.33390541464506 21.396102453340085 30-31 21.173096158726842 26.155685024558473 31.33936969081707 21.331849125897616 32-33 21.102211967170316 26.074365258714145 31.226440217802075 21.596982556313467 34-35 21.405054005297238 26.092231220565065 31.12608215403868 21.376632620099016 36-37 21.62410914730071 25.846214424209172 30.88908918884118 21.64058723964894 38-39 21.332886205124357 26.157392115390426 30.936588038536627 21.573133640948583 40-41 21.550766612424045 25.99648418255378 30.840940985026048 21.611808219996117 42-43 21.5557345452211 26.123044031955377 30.50938775083643 21.811833671987088 44-45 21.612214738774252 26.325182075082097 30.040060530272505 22.022542655871145 46-47 21.68686210446091 26.657455367373338 29.37749457137973 22.27818795678602 48-49 21.934766908706145 26.730843517839446 29.044475920151232 22.289913653303177 50-51 22.059636678507413 26.904134743772413 28.68149699129346 22.354731586426716 52-53 22.207852893908075 27.067503745356092 28.41030814558033 22.3143352151555 54-55 21.916784140459466 26.964648879324276 28.567387035745252 22.551179944471006 56-57 22.026304867434337 27.300602849426486 28.3259731360263 22.347119147112878 58-59 22.23739605571563 26.686323395488408 28.42922831166117 22.647052237134798 60-61 22.20756676784902 26.77387293273184 28.773894580007397 22.244665719411746 62-63 22.082385798491483 26.88037379619687 28.716510638329634 22.32072976698201 64-65 21.932800097935864 26.65548092797963 28.83690524910476 22.574813724979748 66-67 22.083356193602782 27.09643600001642 28.399583327268363 22.420624479112433 68-69 22.113614101260904 26.85842399889673 28.586725706820033 22.441236193022338 70-71 21.883939998230893 26.898587365978173 28.67497026298827 22.542502372802662 72-73 22.25454868474116 26.82132608457511 28.38755874515515 22.536566485528578 74-75 21.964218364014798 27.080010308957053 28.3505276808747 22.605243646153447 76-77 22.047363410915032 26.972130267388422 28.4910505692802 22.489455752416347 78-79 22.0336234411224 27.0887658173812 28.384462832804868 22.493147908691533 80-81 21.75072832243517 27.376861675375807 28.420316178994042 22.452093823194982 82-83 22.05228048790046 27.256293301613805 28.1473412813788 22.544084929106933 84-85 21.948653332173834 26.894055462642157 28.184309658021057 22.972981547162952 86-87 21.87374326342542 26.78511878335863 28.42529822504708 22.915839728168873 88-89 21.980233051582847 26.8240348954082 28.39708269371232 22.798649359296626 90-91 21.790819390435114 27.093786074904795 28.48344785861791 22.631946676042176 92-93 21.756679129672367 26.78137185630106 28.55847926695579 22.903469747070783 94-95 21.623321932373965 26.799887502073876 28.44109190173564 23.13569866381652 96-97 21.916405204313886 26.79041058360786 28.26503212571692 23.028152086361334 98-99 21.768593101129085 26.64239658701072 28.152906727090553 23.43610358476964 100-101 21.51233227037389 27.005962724759193 28.416903294577118 23.064801710289807 102-103 21.90691988308208 26.611610316142315 28.26009105683896 23.22137874393665 104-105 21.718874536703634 26.475928183628184 28.48950968823824 23.315687591429942 106-107 21.57739250522544 26.757035682293225 28.355255300089578 23.31031651239176 108-109 21.667052045990964 26.828354288450733 27.949892103120415 23.554701562437888 110-111 21.51375240245057 26.74286953417419 28.19853203948575 23.54484602388949 112-113 21.56403256228598 26.709420172067805 28.20449273850423 23.52205452714199 114-115 21.314978580274147 26.73408711061745 28.114295268998763 23.836639040109638 116-117 21.136693399769527 26.64602665845545 28.265875404145728 23.95140453762929 118-119 21.20862126270337 26.83289916486253 27.951921043855588 24.006558528578513 120-121 21.054640395717847 26.91271060491504 28.0425438119254 23.99010518744171 122-123 21.174300352984318 26.846048264517098 27.81648921032472 24.163162172173863 124-125 21.060020673553247 26.645892339644195 27.886548138045885 24.407538848756666 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 499.0 1 366.0 2 441.5 3 835.5 4 730.5 5 654.0 6 1678.0 7 3726.0 8 5685.0 9 6373.0 10 5166.5 11 3607.5 12 3273.5 13 3549.5 14 3915.0 15 4221.5 16 4623.0 17 4979.0 18 5364.0 19 5750.5 20 6129.0 21 6701.5 22 7513.5 23 8569.0 24 9925.0 25 11999.5 26 15755.5 27 20109.0 28 23421.5 29 27492.0 30 32863.5 31 38453.5 32 43729.5 33 49380.5 34 56935.0 35 63084.5 36 68200.5 37 74772.0 38 78302.0 39 78428.5 40 78747.5 41 82255.5 42 87093.5 43 91372.5 44 95532.0 45 97690.5 46 97159.5 47 97214.5 48 102956.0 49 104473.5 50 100032.0 51 94208.5 52 87919.0 53 84657.5 54 81176.5 55 73955.0 56 65076.5 57 58213.5 58 54696.5 59 51903.5 60 45817.0 61 37650.0 62 29170.0 63 21337.5 64 16224.5 65 13398.5 66 10064.0 67 7618.0 68 5710.0 69 3892.0 70 2971.5 71 2392.0 72 2059.5 73 1993.5 74 1660.5 75 1264.0 76 924.5 77 591.0 78 400.5 79 265.5 80 187.0 81 148.0 82 99.0 83 59.0 84 47.0 85 30.5 86 23.0 87 15.5 88 7.5 89 5.5 90 2.5 91 1.0 92 1.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05900748734474144 2 0.07427254890324825 3 0.0 4 0.007613869334805349 5 0.030380831561429185 6 5.598433334415697E-4 7 0.0 8 0.0 9 4.8519755564936044E-4 10-11 0.058970164455845346 12-13 0.00979725833522747 14-15 0.007688515112597558 16-17 0.05320377812139717 18-19 7.46457777922093E-5 20-21 0.010748992002078139 22-23 0.012876396669156102 24-25 0.0 26-27 0.0 28-29 0.05150558667662441 30-31 3.732288889610465E-5 32-33 0.0037136274451624123 34-35 1.8661444448052326E-5 36-37 2.2393733337662788E-4 38-39 0.0 40-41 3.732288889610465E-5 42-43 0.0031724455561688947 44-45 1.8661444448052326E-5 46-47 1.1196866668831394E-4 48-49 2.2393733337662788E-4 50-51 5.038590000974127E-4 52-53 1.1196866668831394E-4 54-55 0.0 56-57 0.0016795300003247093 58-59 3.732288889610465E-5 60-61 0.0 62-63 0.0 64-65 6.158276667857267E-4 66-67 0.0 68-69 5.411818889935174E-4 70-71 0.0 72-73 0.0021460661115260174 74-75 0.0036389816673702033 76-77 6.158276667857267E-4 78-79 0.0011010252224350871 80-81 0.0017728372225649708 82-83 1.492915555844186E-4 84-85 1.492915555844186E-4 86-87 3.9189033340909877E-4 88-89 0.0 90-91 0.0 92-93 0.007165994668052092 94-95 0.005990323667824796 96-97 0.005934339334480639 98-99 0.0010263794446428779 100-101 3.3590600006494183E-4 102-103 2.0527588892857557E-4 104-105 5.78504777889622E-4 106-107 0.004516069556428662 108-109 0.005318511667694912 110-111 9.517336668506686E-4 112-113 0.003228429889513052 114-115 5.598433334415697E-4 116-117 0.002183389000422122 118-119 0.002295357667110436 120-121 0.012745766558019738 122-123 8.770878890584593E-4 124-125 0.001959451667045494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2679321.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.265296052908035 #Duplication Level Percentage of deduplicated Percentage of total 1 74.31225485005076 32.151417064465974 2 11.306433288307714 9.783523670421756 3 4.764682209589708 6.184361591877682 4 2.5817212100396683 4.467957299137529 5 1.6038608856865244 3.4695758023453394 6 1.0954557214242135 2.8437129660162337 7 0.7649829456707192 2.3168049533908537 8 0.5560861576506326 1.9247385793342973 9 0.4203830011196264 1.6369195499145544 >10 2.3179397604564356 17.98463950009497 >50 0.16646596688921614 4.988020980561125 >100 0.10213528752861079 8.238732564855077 >500 0.00593922597547994 1.7553552968240929 >1k 0.001397464935406107 0.9015222448185433 >5k 8.734155846288168E-5 0.3171595842210686 >10k+ 1.7468311692576336E-4 1.0355583517209774 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15094 0.5633516849978035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12310 0.45944476231104825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8393 0.3132510065050063 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3581 0.13365326513695075 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3214 0.11995576491208033 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.7322888896104644E-5 0.0 0.0 0.0 0.0 9 3.7322888896104644E-5 0.0 0.0 0.0 0.0 10-11 3.7322888896104644E-5 0.0 0.0 0.0 0.0 12-13 3.7322888896104644E-5 0.0 0.0 0.0 0.0 14-15 3.7322888896104644E-5 0.0 0.0 1.8661444448052322E-5 0.0 16-17 3.7322888896104644E-5 0.0 0.0 1.3063011113636626E-4 0.0 18-19 3.7322888896104644E-5 0.0 0.0 2.4259877782468022E-4 0.0 20-21 3.7322888896104644E-5 0.0 0.0 3.5456744451299414E-4 0.0 22-23 3.7322888896104644E-5 0.0 0.0 6.158276667857267E-4 0.0 24-25 3.7322888896104644E-5 0.0 0.0 9.703951112987208E-4 0.0 26-27 3.7322888896104644E-5 0.0 0.0 0.0014742541113961336 0.0 28-29 3.7322888896104644E-5 0.0 0.0 0.002873862445000058 0.0 30-31 3.7322888896104644E-5 0.0 0.0 0.006139615223409214 0.0 32-33 3.7322888896104644E-5 0.0 0.0 0.012577813557987266 0.0 34-35 3.7322888896104644E-5 0.0 0.0 0.0200797142261043 0.0 36-37 3.7322888896104644E-5 0.0 0.0 0.03273217356188378 0.0 38-39 3.7322888896104644E-5 0.0 0.0 0.054678032232793314 0.0 40-41 3.7322888896104644E-5 0.0 0.0 0.08520815534980691 0.0 42-43 3.7322888896104644E-5 0.0 0.0 0.11170740646604121 0.0 44-45 3.7322888896104644E-5 0.0 0.0 0.14184563924964572 0.0 46-47 3.7322888896104644E-5 0.0 0.0 0.17870199203454906 0.0 48-49 3.7322888896104644E-5 0.0 0.0 0.21964520115357583 0.0 50-51 3.7322888896104644E-5 0.0 0.0 0.26732519171834956 0.0 52-53 3.7322888896104644E-5 0.0 0.0 0.31304573061607777 0.0 54-55 3.7322888896104644E-5 0.0 0.0 0.36031516940299424 0.0 56-57 3.7322888896104644E-5 0.0 0.0 0.4155343835247811 0.0 58-59 3.7322888896104644E-5 0.0 0.0 0.4787966802036785 0.0 60-61 3.7322888896104644E-5 0.0 0.0 0.5549726964406281 0.0 62-63 3.7322888896104644E-5 0.0 0.0 0.6311860355664738 0.0 64-65 3.7322888896104644E-5 0.0 0.0 0.707567327692352 0.0 66-67 3.7322888896104644E-5 0.0 0.0 0.7950521792648212 0.0 68-69 3.7322888896104644E-5 0.0 0.0 0.9025980836189468 0.0 70-71 3.7322888896104644E-5 0.0 0.0 1.0276670843097935 0.0 72-73 3.7322888896104644E-5 0.0 0.0 1.155535301667848 0.0 74-75 3.7322888896104644E-5 0.0 0.0 1.2802310734697335 0.0 76-77 3.7322888896104644E-5 0.0 0.0 1.4192961574966194 0.0 78-79 3.7322888896104644E-5 0.0 0.0 1.5855509660843174 0.0 80-81 3.7322888896104644E-5 0.0 0.0 1.7789395148994838 0.0 82-83 3.7322888896104644E-5 0.0 0.0 1.986902651828579 0.0 84-85 3.7322888896104644E-5 3.7322888896104644E-5 0.0 2.195593585091148 0.0 86-87 3.7322888896104644E-5 3.7322888896104644E-5 0.0 2.4121409864663472 0.0 88-89 3.7322888896104644E-5 3.7322888896104644E-5 0.0 2.6600022916253785 0.0 90-91 3.7322888896104644E-5 3.7322888896104644E-5 0.0 2.9475564891254167 0.0 92-93 3.7322888896104644E-5 3.7322888896104644E-5 0.0 3.2643904929644485 0.0 94-95 3.7322888896104644E-5 3.7322888896104644E-5 0.0 3.5757193706913055 0.0 96-97 3.7322888896104644E-5 3.7322888896104644E-5 0.0 3.885163442528909 0.0 98-99 3.7322888896104644E-5 3.7322888896104644E-5 0.0 4.228104807150768 0.0 100-101 3.7322888896104644E-5 3.7322888896104644E-5 0.0 4.636230597229671 0.0 102-103 3.7322888896104644E-5 3.7322888896104644E-5 0.0 5.0774804512038685 0.0 104-105 5.598433334415696E-5 3.7322888896104644E-5 0.0 5.5165282547331955 0.0 106-107 7.464577779220929E-5 3.7322888896104644E-5 0.0 5.959681576041094 0.0 108-109 7.464577779220929E-5 3.7322888896104644E-5 0.0 6.423642407908571 0.0 110-111 7.464577779220929E-5 3.7322888896104644E-5 0.0 6.9536460916777045 0.0 112-113 7.464577779220929E-5 3.7322888896104644E-5 0.0 7.54075379545788 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9750 0.0 52.864754 1 GTATCAA 16155 0.0 48.6142 1 ATCAACG 21125 0.0 36.726402 3 TATCAAC 21185 0.0 36.518093 2 TCAACGC 21260 0.0 36.411957 4 CAACGCA 21525 0.0 35.393852 5 AACGCAG 21970 0.0 34.77631 6 GTACATG 18140 0.0 32.38876 1 TACATGG 18330 0.0 31.09402 2 ACATGGG 18715 0.0 29.057209 3 ACGCAGA 26220 0.0 28.776026 7 CGCAGAG 26170 0.0 28.762735 8 TACCGTA 110 7.7154575E-4 27.068499 7 TATAACG 135 8.197337E-5 26.46945 2 GCAGAGT 28025 0.0 26.13741 9 CATGGGA 13665 0.0 25.908205 4 GAGTACT 15460 0.0 25.752497 12-13 AGTACTT 15700 0.0 25.358828 12-13 ACCGTAT 165 1.1032393E-5 25.263931 8 GTACTTT 16350 0.0 24.16855 14-15 >>END_MODULE