FastQCFastQC Report
Thu 26 May 2016
SRR1364600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666165
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108100.038.6905171
GTACATG171300.034.432691
ATCAACG124750.033.1440933
TCAACGC124500.032.9713754
TACATGG174500.032.639962
CAACGCA127200.032.3651855
AACGCAG127200.031.4451376
TATCAAC132700.031.2931352
ACATGGG182300.030.3610123
GGTATCA71450.027.6840081
ACGCAGA148400.026.547177
CGCAGAG146750.024.975818
CGCATAG3451.8189894E-1224.144938
AGTACAT124600.023.2861732
TAGCCCT30350.023.1638374
CATGGGA108350.022.5995664
GTGTAGC25200.022.2239071
CATGGGG79100.021.9934624
GAGTACA142000.021.8176571
CATGGGT42350.021.524114
Produced by FastQC (version 0.11.5)