##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364600_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3666165 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.90187266530557 30.0 26.0 31.0 7.0 34.0 2 25.689134558864644 30.0 25.0 31.0 7.0 34.0 3 26.235678972441228 31.0 26.0 31.0 7.0 34.0 4 29.60618057288747 35.0 29.0 37.0 7.0 37.0 5 29.708034690200794 35.0 30.0 37.0 7.0 37.0 6 29.911765018759386 35.0 30.0 37.0 2.0 37.0 7 29.694455923287688 35.0 30.0 37.0 2.0 37.0 8 29.535814945590282 35.0 30.0 37.0 2.0 37.0 9 30.4085184382045 37.0 29.0 39.0 2.0 39.0 10-11 30.357422265500873 37.0 28.5 39.0 2.0 39.0 12-13 30.32614489527885 37.0 28.0 39.0 2.0 39.0 14-15 30.920451752717078 37.0 27.5 40.0 2.0 41.0 16-17 31.30523176125461 37.5 29.5 40.0 2.0 41.0 18-19 31.12791950171364 37.0 28.5 40.0 2.0 41.0 20-21 31.036663516235627 37.0 27.5 40.0 2.0 41.0 22-23 31.092453422036378 37.0 28.0 40.0 2.0 41.0 24-25 31.181780689085187 37.0 29.0 40.0 2.0 41.0 26-27 30.98644714572312 37.0 28.5 40.0 2.0 41.0 28-29 30.874318258998166 37.0 28.0 40.0 2.0 41.0 30-31 30.738732572047354 37.0 28.0 40.0 2.0 41.0 32-33 30.628043200456062 37.0 28.0 40.0 2.0 41.0 34-35 30.67565262338165 37.0 28.0 40.0 2.0 41.0 36-37 30.613567174417952 37.0 28.0 40.0 2.0 41.0 38-39 30.31965759860781 37.0 27.0 40.0 2.0 41.0 40-41 30.18106590947216 37.0 27.0 40.0 2.0 41.0 42-43 29.680934982468056 36.0 25.5 40.0 2.0 41.0 44-45 29.544152268105773 36.0 26.0 40.0 2.0 41.0 46-47 29.50207055601698 36.0 26.0 40.0 2.0 41.0 48-49 29.4315501348139 36.0 26.0 40.0 2.0 41.0 50-51 29.19938300649316 35.0 25.5 40.0 2.0 41.0 52-53 29.10176151373438 35.0 25.5 40.0 2.0 41.0 54-55 28.928842537092574 35.0 25.5 39.5 2.0 41.0 56-57 28.70612820208583 35.0 24.5 39.0 2.0 41.0 58-59 28.489957898785242 35.0 24.0 39.0 2.0 40.5 60-61 28.205195210799296 34.0 23.0 39.0 2.0 40.0 62-63 28.016044286059138 34.0 23.0 39.0 2.0 40.0 64-65 27.792551344524863 34.0 22.5 38.0 2.0 40.0 66-67 27.52336065070721 34.0 22.0 38.0 2.0 40.0 68-69 27.276011990731458 34.0 22.0 37.5 2.0 40.0 70-71 26.840166904653774 33.0 20.5 37.0 2.0 39.5 72-73 26.60220298322634 33.0 20.5 36.5 2.0 39.0 74-75 26.576769594385414 33.0 21.0 36.0 2.0 39.0 76-77 26.32447898553393 33.0 20.0 36.0 2.0 39.0 78-79 26.034897501885485 33.0 20.0 35.5 2.0 37.5 80-81 25.638628103208667 32.5 18.0 35.0 2.0 37.0 82-83 25.7380692085599 33.0 20.0 35.0 2.0 37.0 84-85 25.57368803640862 33.0 18.5 35.0 2.0 36.0 86-87 25.445630106664595 33.0 18.0 35.0 2.0 36.0 88-89 25.27821729245683 33.0 18.0 35.0 2.0 36.0 90-91 25.144594692273806 33.0 17.5 35.0 2.0 35.5 92-93 25.024230496990725 33.0 16.0 35.0 2.0 35.0 94-95 24.9147483814831 33.0 13.5 35.0 2.0 35.0 96-97 24.795227710700416 32.5 10.5 35.0 2.0 35.0 98-99 24.706784337311603 32.0 7.5 35.0 2.0 35.0 100-101 24.602424740839542 32.0 7.0 35.0 2.0 35.0 102-103 23.51219421384471 30.5 6.5 34.0 2.0 35.0 104-105 24.320219902813974 32.0 3.5 34.5 2.0 35.0 106-107 24.471914930179082 32.0 2.0 35.0 2.0 35.0 108-109 24.42611475479145 32.0 2.0 35.0 2.0 35.0 110-111 24.362747585010496 32.0 2.0 35.0 2.0 35.0 112-113 24.268362580516698 32.0 2.0 35.0 2.0 35.0 114-115 24.16196747827771 32.0 2.0 35.0 2.0 35.0 116-117 24.052511684553203 32.0 2.0 35.0 2.0 35.0 118-119 23.920345783673127 32.0 2.0 35.0 2.0 35.0 120-121 23.734328651329115 32.0 2.0 35.0 2.0 35.0 122-123 23.522195400370684 32.0 2.0 35.0 2.0 35.0 124-125 22.603831524222176 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3809145385578923 1101 2 1.8320833510774328 1101 3 1.3347008688725879 1101 4 1.5466891224999095 1101 5 1.2331346892059578 1101 6 1.1783075162503174 1101 7 1.2309752949525148 1101 8 1.3828577484947964 1101 9 1.7539004224392585 1101 10-11 1.7072028031323718 1101 12-13 1.883039993886463 1101 14-15 2.1441293554661556 1101 16-17 1.7237867918774512 1101 18-19 1.5825130201990163 1101 20-21 1.8786610150194356 1101 22-23 1.6264634974578414 1101 24-25 1.6103463312701471 1101 26-27 1.869399213746341 1101 28-29 1.8788163380073328 1101 30-31 1.9390437764329 1101 32-33 1.970569941154725 1101 34-35 1.728083388752765 1101 36-37 1.6475305997809286 1101 38-39 1.5879681434428683 1101 40-41 1.6601593445213823 1101 42-43 1.8212090794017257 1101 44-45 1.7472821216997971 1101 46-47 1.3719585824356315 1101 48-49 1.249056995230017 1101 50-51 1.275422894908786 1101 52-53 1.1823154767099986 1101 54-55 1.24645166825227 1101 56-57 1.2512462059800882 1101 58-59 1.1449876603417728 1101 60-61 1.2577550558387465 1101 62-63 1.142372092907312 1101 64-65 1.1018766384808707 1101 66-67 1.0901401024653126 1101 68-69 0.907547374746045 1101 70-71 0.8156973331852093 1101 72-73 0.8169777205829192 1101 74-75 0.8469966695059341 1101 76-77 0.9684221047778756 1101 78-79 0.9613851062095051 1101 80-81 1.0840598378630482 1101 82-83 1.1008968281022433 1101 84-85 1.1879932509589217 1101 86-87 1.0691600989698067 1101 88-89 1.0264016936960267 1101 90-91 1.1195440306923246 1101 92-93 1.1147645366453105 1101 94-95 1.092546401117552 1101 96-97 1.0572167104675678 1101 98-99 1.117358260531212 1101 100-101 1.133414747353747 1101 102-103 1.0276300235642886 1101 104-105 1.0820559470447364 1101 106-107 1.129166368258609 1101 108-109 1.112204256050191 1101 110-111 1.1373528543775286 1101 112-113 1.1055960304384094 1101 114-115 1.142916167764028 1101 116-117 1.0958491751813924 1101 118-119 0.9860176941082308 1101 120-121 1.016798471250933 1101 122-123 0.9999462581994472 1101 124-125 0.8422246951834573 1102 1 0.6801700298734623 1102 2 1.1493996705072576 1102 3 0.78764220459637 1102 4 0.6809209800515852 1102 5 0.46260088901303575 1102 6 0.4035096115033099 1102 7 0.4884822487137619 1102 8 0.41980904308760714 1102 9 1.044073008075305 1102 10-11 1.0183986595529895 1102 12-13 1.1580725316734153 1102 14-15 1.3946252433717277 1102 16-17 1.077722009093499 1102 18-19 0.8229036942375743 1102 20-21 1.206164369068663 1102 22-23 1.1274013778591652 1102 24-25 0.8136817301763948 1102 26-27 1.185932821419847 1102 28-29 0.841044379262641 1102 30-31 0.8321232548657065 1102 32-33 1.1465657277870633 1102 34-35 0.913299652231629 1102 36-37 0.8425063944750129 1102 38-39 1.1105462090639158 1102 40-41 1.1147730924131949 1102 42-43 1.2917101412666376 1102 44-45 0.8793170018063208 1102 46-47 0.7165790183717853 1102 48-49 0.6920587640021658 1102 50-51 0.6697719987910631 1102 52-53 0.5877850335744235 1102 54-55 0.6001011645934007 1102 56-57 0.5504477016153153 1102 58-59 0.6066904344262518 1102 60-61 0.47328119536318525 1102 62-63 0.3571885176211893 1102 64-65 0.3245601886993619 1102 66-67 0.2871102278636357 1102 68-69 0.14954057239329543 1102 70-71 0.4319222078971805 1102 72-73 0.11722874785449733 1102 74-75 0.18672069492651744 1102 76-77 0.07088344547507575 1102 78-79 0.1997499144621706 1102 80-81 0.30678199223554614 1102 82-83 0.2564676080665862 1102 84-85 0.2673258904201852 1102 86-87 0.35802134661595986 1102 88-89 0.26887729286257667 1102 90-91 0.35288423255695633 1102 92-93 0.45493451524757944 1102 94-95 0.3935195767453976 1102 96-97 0.21401735861627103 1102 98-99 0.3030280476167704 1102 100-101 0.3749326125540584 1102 102-103 0.24140388544363844 1102 104-105 0.3436623611499954 1102 106-107 0.2669705138433933 1102 108-109 0.3282265842917873 1102 110-111 0.35127210852521884 1102 112-113 0.2320875044352526 1102 114-115 0.2614419503253771 1102 116-117 0.34660852390424424 1102 118-119 0.2782006035980338 1102 120-121 0.23853225901030584 1102 122-123 0.12521352929686458 1102 124-125 0.05255230908576536 1103 1 0.8781857246049434 1103 2 1.3630260955992206 1103 3 0.9530478673883671 1103 4 0.6881599162733565 1103 5 0.7956138404339761 1103 6 0.9134821920123244 1103 7 0.7987385542436769 1103 8 1.0327654332993674 1103 9 1.6078183553389351 1103 10-11 1.6244476933358776 1103 12-13 1.5444160867315873 1103 14-15 1.8695274746430144 1103 16-17 1.4880488034091925 1103 18-19 1.4128568639525128 1103 20-21 1.6916894918028795 1103 22-23 1.4734306741013086 1103 24-25 1.4359389642114273 1103 26-27 1.5832373486310374 1103 28-29 1.4923619171176057 1103 30-31 1.6125190846252515 1103 32-33 1.5967015148517056 1103 34-35 1.3725275736871794 1103 36-37 1.266772643995104 1103 38-39 1.3785193348314309 1103 40-41 1.3695136079105161 1103 42-43 1.5515926281899617 1103 44-45 1.2825882634841115 1103 46-47 1.1024882045819915 1103 48-49 1.006321868585605 1103 50-51 1.0128684373885548 1103 52-53 0.9101786332879733 1103 54-55 0.9993228608442557 1103 56-57 0.9004611969333034 1103 58-59 0.981702585317862 1103 60-61 1.0106386521305168 1103 62-63 0.8665537918024846 1103 64-65 0.6826419441185898 1103 66-67 0.7278975716038509 1103 68-69 0.7206522530419974 1103 70-71 0.8348308318308284 1103 72-73 0.7270817084515713 1103 74-75 0.704535756994769 1103 76-77 0.65809572121114 1103 78-79 0.7054711804965805 1103 80-81 0.7905119653878643 1103 82-83 0.8008128656110287 1103 84-85 0.7368821037803137 1103 86-87 0.7921757841079575 1103 88-89 0.8255316929148577 1103 90-91 0.8410686987482059 1103 92-93 0.7129241178964065 1103 94-95 0.6835520165627464 1103 96-97 0.8036846249992138 1103 98-99 0.8144942858368722 1103 100-101 0.7783668901852501 1103 102-103 0.6600591799649109 1103 104-105 0.6402188086785614 1103 106-107 0.7876527949163226 1103 108-109 0.8608628750908736 1103 110-111 0.7633421813897279 1103 112-113 0.9049495601618993 1103 114-115 0.8609149522014476 1103 116-117 0.9504192919767362 1103 118-119 0.9225273114620087 1103 120-121 0.9102128192174632 1103 122-123 0.7157427261535609 1103 124-125 0.5880889190074257 1104 1 1.0732780699608 1104 2 1.5283681300918879 1104 3 1.050675407609365 1104 4 1.1733695477471073 1104 5 0.6982515497719817 1104 6 0.873877079886455 1104 7 0.630269031583996 1104 8 1.0393626778378149 1104 9 1.3459474582416924 1104 10-11 1.450971294770227 1104 12-13 1.4629917756102557 1104 14-15 1.4309017243087894 1104 16-17 1.2492413720660025 1104 18-19 1.1734747307644398 1104 20-21 1.4370533677210595 1104 22-23 1.3474594258255905 1104 24-25 1.2096674129337366 1104 26-27 1.4688776238850494 1104 28-29 1.5130586198166043 1104 30-31 1.5251728198170902 1104 32-33 1.4831511498364485 1104 34-35 1.4098313720077513 1104 36-37 1.2429896846625397 1104 38-39 1.396369982797264 1104 40-41 1.3729347296631822 1104 42-43 1.5535158036846504 1104 44-45 1.3219552983618712 1104 46-47 1.2791845136891524 1104 48-49 1.1427534274270492 1104 50-51 1.1493634868159361 1104 52-53 1.0574200743918176 1104 54-55 0.963578607099155 1104 56-57 0.9586435881072894 1104 58-59 1.0542415673757048 1104 60-61 0.9842414741467991 1104 62-63 0.8958084943219511 1104 64-65 0.8706285724866305 1104 66-67 0.8418085797460506 1104 68-69 0.8099753906116405 1104 70-71 0.7999641922711866 1104 72-73 0.7684239396073664 1104 74-75 0.7567696529271757 1104 76-77 0.8821199381307068 1104 78-79 0.7774794310525763 1104 80-81 0.9337412330308439 1104 82-83 0.9951296962831506 1104 84-85 0.9566190711631819 1104 86-87 0.8573045931447432 1104 88-89 0.8421811985879444 1104 90-91 0.7898118274899169 1104 92-93 0.8561692706350001 1104 94-95 0.7882689737047883 1104 96-97 0.7142317674481191 1104 98-99 0.8260072288202309 1104 100-101 0.7691194406975335 1104 102-103 0.7545430315452037 1104 104-105 0.8544690607154379 1104 106-107 0.8263445389424646 1104 108-109 0.8603501533369311 1104 110-111 0.8372286411666074 1104 112-113 0.8713532302862568 1104 114-115 0.8171627677933131 1104 116-117 0.7574706377846141 1104 118-119 0.8257992129089509 1104 120-121 0.8766316606870639 1104 122-123 0.8107703725274966 1104 124-125 0.5667496583811129 1105 1 1.1325788573100084 1105 2 1.538392664514177 1105 3 0.9408660349708633 1105 4 1.1376189602046836 1105 5 0.9610065318327763 1105 6 0.8961886806380122 1105 7 0.8851093084503248 1105 8 0.9412819324594608 1105 9 1.1083657174663202 1105 10-11 1.0515140031613441 1105 12-13 1.2077954962353346 1105 14-15 1.4838042662041033 1105 16-17 1.0973372326435964 1105 18-19 1.1434851203656962 1105 20-21 1.1691344708764184 1105 22-23 1.3148310741240863 1105 24-25 0.95679780314887 1105 26-27 1.3409386768205422 1105 28-29 1.3370484473114885 1105 30-31 1.3334983930556241 1105 32-33 1.4462187471496328 1105 34-35 1.2308097178662045 1105 36-37 0.8518920814007913 1105 38-39 1.121699907390724 1105 40-41 1.2840308046192241 1105 42-43 1.4575064880538697 1105 44-45 1.238251753220183 1105 46-47 0.8627663698867742 1105 48-49 0.9799979497859539 1105 50-51 1.04971448556212 1105 52-53 0.8336541692615533 1105 54-55 0.9150906625696216 1105 56-57 1.0909412598550396 1105 58-59 1.0094788999834634 1105 60-61 0.9180650536338035 1105 62-63 0.8352242499068616 1105 64-65 0.5880047049875365 1105 66-67 0.5836954106267136 1105 68-69 0.4496820198614593 1105 70-71 0.6675547591718889 1105 72-73 0.6066291204038521 1105 74-75 0.6654539943321254 1105 76-77 0.6707254438999755 1105 78-79 0.6439694579785744 1105 80-81 0.7303318483705254 1105 82-83 0.7131247766095186 1105 84-85 0.66664127156211 1105 86-87 0.7394605049461553 1105 88-89 0.7709527845072586 1105 90-91 0.5790584063472117 1105 92-93 0.6950736353066489 1105 94-95 0.5595483366379099 1105 96-97 0.5773103474602799 1105 98-99 0.6596749722816497 1105 100-101 0.7039829394976671 1105 102-103 0.6521452062155149 1105 104-105 0.6587211077418544 1105 106-107 0.6723455176336337 1105 108-109 0.7491278652682496 1105 110-111 0.7573601681790088 1105 112-113 0.6686485608167381 1105 114-115 0.7265238480816407 1105 116-117 0.6675777422799598 1105 118-119 0.6784157250371621 1105 120-121 0.5730957591713413 1105 122-123 0.6181430407622237 1105 124-125 0.6235654941860851 1106 1 0.15065779523553502 1106 2 -2.372840123955104 1106 3 -0.37480029840338247 1106 4 0.22711258764838504 1106 5 0.12963978013614863 1106 6 0.3537228222640998 1106 7 0.4020669610293268 1106 8 0.454718203378512 1106 9 0.936086630536412 1106 10-11 1.0617148620082268 1106 12-13 1.0567040352184307 1106 14-15 1.1987640404929536 1106 16-17 0.7210987411996257 1106 18-19 0.7458218155485348 1106 20-21 1.0503366219806232 1106 22-23 0.6756774753086141 1106 24-25 0.8543913745400253 1106 26-27 0.8928024433480282 1106 28-29 0.9895667073843413 1106 30-31 1.0912814486148683 1106 32-33 1.161426497253803 1106 34-35 0.988448694160752 1106 36-37 0.7128494150197291 1106 38-39 1.0827139189624084 1106 40-41 1.0072061458791346 1106 42-43 1.2815313731930686 1106 44-45 1.1449914263017504 1106 46-47 0.8517424689895456 1106 48-49 0.6995278433017624 1106 50-51 0.8233701822436039 1106 52-53 0.6464214058087485 1106 54-55 0.5757294270058857 1106 56-57 0.5869230666230898 1106 58-59 0.5758585547091926 1106 60-61 0.7068963698834914 1106 62-63 0.6166209041095918 1106 64-65 0.49375380380808664 1106 66-67 0.47961960132418113 1106 68-69 0.3997799359108605 1106 70-71 0.48502431138479096 1106 72-73 0.3710929821503015 1106 74-75 0.46159496842678394 1106 76-77 0.32226094736046207 1106 78-79 0.3319408328762634 1106 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 377397.0 3 48690.0 4 28369.0 5 22445.0 6 22167.0 7 24892.0 8 26656.0 9 28863.0 10 30370.0 11 32589.0 12 34687.0 13 35449.0 14 34083.0 15 28377.0 16 23505.0 17 20596.0 18 18753.0 19 17558.0 20 17287.0 21 19058.0 22 23486.0 23 32331.0 24 47513.0 25 60655.0 26 67207.0 27 73832.0 28 84207.0 29 95822.0 30 111174.0 31 127804.0 32 152980.0 33 191194.0 34 246329.0 35 332557.0 36 473877.0 37 524295.0 38 128922.0 39 189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89161729478919 18.533012921783794 14.223747262635063 23.35162252079195 2 13.510128836902293 20.493183002650674 41.79972448732385 24.196963673123186 3 19.58830745458863 25.60770091911584 27.77028779411105 27.033703832184486 4 11.732210793061682 17.032728661322036 37.35985114980321 33.875209395813066 5 13.383830783013288 39.0490109107108 33.13294518047795 14.434213125797973 6 31.352755993772135 37.03799188299927 17.993085922922667 13.616166200305926 7 28.491278177360357 33.10788073555756 20.32061251084231 18.080228576239783 8 24.762268913741188 37.499812099995054 19.667104052326668 18.07081493393709 9 25.73412089975419 17.99407248038053 19.581449552948918 36.69035706691636 10-11 23.903675538656525 28.409870635024692 27.340642891482013 20.345810934836763 12-13 24.92791062392761 25.47047300029508 27.519207440355842 22.082408935421473 14-15 21.870553846948425 26.90510378234108 25.58628588980174 25.638056480908755 16-17 21.892215516513648 30.046508616415107 25.848111557298292 22.213164309772957 18-19 21.851067530493992 28.56912533726034 27.94560276194472 21.63420437030095 20-21 23.072227379058823 27.495886902876805 27.841643916318798 21.590241801745577 22-23 22.49073608937521 27.57924545113154 27.537729356607127 22.39228910288612 24-25 22.243526240060994 27.453606599265683 27.927016734555004 22.375850426118312 26-27 22.16833051502399 27.635987356365682 27.840043069618055 22.35563905899228 28-29 21.975727320405408 27.68475380253629 27.94009822788974 22.399420649168565 30-31 22.345043206036074 27.403779239112097 27.939311448831255 22.311866106020574 32-33 22.131947456260235 27.39514850107906 27.93358601158341 22.539318031077293 34-35 22.456139152763456 27.405896372986227 27.842244364162838 22.295720110087483 36-37 22.451892207293582 27.318883228028213 27.65704153413892 22.572183030539282 38-39 22.239053452995233 27.42030198935219 27.92928769873096 22.411356858921614 40-41 22.63839519556815 27.211630297843126 27.861039248374155 22.288935258214572 42-43 22.25847533011714 27.22168837773526 27.94909954254078 22.57073674960682 44-45 22.52214124220463 27.51922459546041 27.525959522134414 22.432674640200545 46-47 22.48852804524994 27.60063187096501 27.150550155270064 22.760289928514986 48-49 22.49064018038168 27.53721833632681 27.325113875699518 22.647027607591998 50-51 22.60791321699903 27.708867904321444 27.105672390127904 22.57754648855162 52-53 22.664225712894815 27.749430693850535 26.970922381850954 22.615421211403692 54-55 22.622945937476008 27.566876311303567 27.081876708844227 22.728301042376195 56-57 22.521128115119676 27.772009796582125 27.05013480682788 22.656727281470317 58-59 22.698301008730827 27.63373147366371 26.969707481991634 22.69826003561383 60-61 22.779006751196057 27.492181657372534 27.253509631638078 22.47530195979333 62-63 22.62940618584186 27.567814370112508 27.199063830624148 22.60371561342149 64-65 22.60475427227862 27.530698893539657 27.067989349486105 22.796557484695622 66-67 22.539562413474503 27.813544911870647 26.963440869612477 22.68345180504237 68-69 22.612828095436758 27.615289600703562 27.041134108679042 22.730748195180638 70-71 22.562088015110422 27.518067622980762 27.132960033889468 22.78688432801935 72-73 22.74450174514399 27.578818195372023 26.896625545238035 22.780054514245958 74-75 22.626811188559127 27.693169325343014 26.86318061395408 22.816838872143784 76-77 22.6440737220456 27.556004391858675 27.034744299869995 22.765177586225732 78-79 22.64986038314808 27.5846735303766 26.998809917590137 22.766656168885177 80-81 22.461901859813704 27.80889016246155 27.049317583889394 22.67989039383535 82-83 22.559930220500377 27.853736770878808 26.956442993831192 22.629890014789623 84-85 22.67006141243129 27.566667613546354 26.993500399328298 22.769770574694057 86-87 22.530514804869583 27.612584067428518 27.0741345185407 22.782766609161197 88-89 22.703512693739086 27.489930997365704 27.074276011903176 22.732280296992037 90-91 22.593314305546087 27.62960910175996 27.145382556453374 22.631694036240575 92-93 22.56617007130147 27.60970707447619 27.15641743293941 22.667705421282925 94-95 22.474451210582572 27.723619529318675 27.044886744902264 22.75704251519649 96-97 22.594489748528186 27.663770847846575 26.995219167042308 22.746520236582935 98-99 22.597751513719427 27.51418108905847 26.862281239713333 23.025786157508772 100-101 22.33480355486073 27.661400034358884 27.12957477741152 22.874221633368865 102-103 22.462445149973497 27.433346630309458 27.249206279815734 22.855001939901314 104-105 22.352812813269367 27.538385718037116 27.157502057566525 22.951299411126985 106-107 22.33996658224598 27.634201917253158 27.077721884258764 22.948109616242093 108-109 22.300075965901936 27.686552833451657 26.895002339618664 23.118368861027744 110-111 22.150163024168652 27.893108907354367 27.030783561964068 22.92594450651291 112-113 22.392483868637825 27.730500743642178 26.96994178250477 22.90707360521523 114-115 22.13028391361009 27.797415020135208 26.98556231469908 23.086738751555625 116-117 22.12642218610321 27.792473882895752 27.046181179081845 23.034922751919186 118-119 22.209564057352456 27.71298981637364 26.960234765652093 23.11721136062182 120-121 22.126946134595464 27.892472040898703 27.02459904027118 22.955982784234653 122-123 22.19436726299492 27.822775825882474 26.966645461396233 23.016211449726374 124-125 22.264136619696387 27.754159588264322 26.87520429627732 23.10649949576197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5450.0 1 3880.5 2 1756.0 3 1118.5 4 978.0 5 873.5 6 843.0 7 986.5 8 1181.5 9 1304.0 10 1388.0 11 1460.5 12 1609.5 13 1799.0 14 1962.0 15 2197.0 16 2485.5 17 2775.5 18 3115.5 19 3611.5 20 4214.0 21 5010.0 22 6339.0 23 7998.5 24 9879.5 25 12755.0 26 16650.0 27 21509.5 28 26872.0 29 33068.5 30 40842.5 31 48720.0 32 56913.5 33 66059.5 34 75459.0 35 83963.0 36 91756.5 37 99296.0 38 105255.5 39 109178.0 40 113991.0 41 120826.5 42 126148.0 43 131331.5 44 136789.0 45 140272.5 46 142476.5 47 145330.5 48 148318.0 49 147610.5 50 143184.0 51 136081.5 52 128621.5 53 122564.0 54 116753.5 55 108403.5 56 97358.0 57 87247.0 58 78452.0 59 68735.5 60 57620.5 61 46810.5 62 37223.0 63 28887.0 64 22177.5 65 17085.5 66 13119.5 67 10124.5 68 7744.5 69 5704.5 70 4381.0 71 3550.5 72 2778.5 73 2216.0 74 1781.0 75 1408.5 76 993.0 77 637.5 78 450.5 79 328.0 80 210.5 81 142.0 82 113.5 83 87.5 84 69.5 85 52.0 86 38.5 87 32.5 88 28.0 89 22.5 90 14.5 91 8.0 92 8.5 93 6.5 94 4.5 95 3.5 96 1.0 97 2.5 98 4.0 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20558267290206522 2 0.24502443288831793 3 0.29766799912169806 4 0.283920663690805 5 0.31616143845135175 6 0.22939502177343357 7 0.21935728479214656 8 0.19922725791119605 9 0.19909087561525463 10-11 0.14197397007499662 12-13 0.16706831252821408 14-15 0.18415701420967143 16-17 0.15733061659799819 18-19 0.18314778521970504 20-21 0.17767885515245493 22-23 0.20029103981953894 24-25 0.19011692054231058 26-27 0.24086477286210523 28-29 0.1692913439520589 30-31 0.15022509897945127 32-33 0.19000781470555744 34-35 0.14838393798424238 36-37 0.14875217018328418 38-39 0.18089747733667197 40-41 0.20120480120234632 42-43 0.26658647387665313 44-45 0.16747745941603828 46-47 0.15295274489827926 48-49 0.1555985614395424 50-51 0.16117659734354564 52-53 0.18183851517866761 54-55 0.16807754151818044 56-57 0.16674099501795472 58-59 0.14260132863632707 60-61 0.15554400852116584 62-63 0.14426519264681212 64-65 0.13968274750318113 66-67 0.12743561732764347 68-69 0.1305860483638898 70-71 0.13230446529275142 72-73 0.10918766613068424 74-75 0.11093335951873416 76-77 0.13215444476721586 78-79 0.13840075392133197 80-81 0.14789296171885336 82-83 0.14977503740284467 84-85 0.13684599574760004 86-87 0.16184487059365849 88-89 0.15813527214405243 90-91 0.14670643574416317 92-93 0.14650186230025106 94-95 0.16663188918120161 96-97 0.15588496426101936 98-99 0.14947499635177358 100-101 0.13125432161400263 102-103 0.16870490007951086 104-105 0.12764019077155556 106-107 0.13467751724213176 108-109 0.14489255120814257 110-111 0.12105292587758598 112-113 0.1124199265444954 114-115 0.1104014685645627 116-117 0.11447929921321054 118-119 0.09871350580238478 120-121 0.09163526464302615 122-123 0.1183661946475404 124-125 0.11467023442752851 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3666165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.15513591128862 #Duplication Level Percentage of deduplicated Percentage of total 1 79.59978745917891 49.47535608034953 2 10.685922940812718 13.283699854475428 3 4.055420276158168 7.561955953260196 4 1.9267622448842188 4.790326767980727 5 1.1052090586550831 3.434720962554702 6 0.6647416446319319 2.479026436079475 7 0.44058378392840003 1.9169181479265662 8 0.2886884639987269 1.4354776572689614 9 0.20984510711903825 1.1738656037972466 >10 0.9583248874465898 10.095921631618513 >50 0.04633578264982769 1.9652894387400817 >100 0.017613745615325156 1.816648806084303 >500 4.497676105918676E-4 0.176314497803209 >1k 3.1483731051053405E-4 0.39447816206102704 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.7276459188279852E-5 0.0 0.0 2.7276459188279852E-5 0.0 6 5.4552918376559705E-5 0.0 0.0 2.7276459188279852E-5 2.7276459188279852E-5 7 8.182937756483955E-5 0.0 0.0 2.7276459188279852E-5 2.7276459188279852E-5 8 8.182937756483955E-5 0.0 0.0 2.7276459188279852E-5 2.7276459188279852E-5 9 8.182937756483955E-5 0.0 0.0 2.7276459188279852E-5 2.7276459188279852E-5 10-11 8.182937756483955E-5 0.0 0.0 4.091468878241978E-5 2.7276459188279852E-5 12-13 1.0910583675311941E-4 0.0 0.0 8.182937756483955E-5 2.7276459188279852E-5 14-15 2.1821167350623882E-4 0.0 0.0 1.0910583675311941E-4 2.7276459188279852E-5 16-17 2.3184990310037872E-4 0.0 0.0 1.2274406634725933E-4 2.7276459188279852E-5 18-19 2.727645918827985E-4 0.0 0.0 1.636587551296791E-4 2.7276459188279852E-5 20-21 3.273175102593582E-4 0.0 0.0 2.045734439120989E-4 2.7276459188279852E-5 22-23 3.5459396944763805E-4 0.0 0.0 2.727645918827985E-4 2.7276459188279852E-5 24-25 4.091468878241978E-4 0.0 0.0 4.227851174183377E-4 2.7276459188279852E-5 26-27 4.091468878241978E-4 0.0 0.0 8.592084644308153E-4 4.091468878241978E-5 28-29 5.046144949831773E-4 0.0 0.0 0.0015138434849495319 5.4552918376559705E-5 30-31 5.728056429538769E-4 0.0 0.0 0.0028367517555811044 5.4552918376559705E-5 32-33 6.000821021421568E-4 0.0 0.0 0.005973544562233287 5.4552918376559705E-5 34-35 6.819114797069962E-4 0.0 0.0 0.010515075017081883 5.4552918376559705E-5 36-37 7.091879388952761E-4 0.0 0.0 0.018275227656147502 5.4552918376559705E-5 38-39 7.36464398083556E-4 0.0 0.0 0.030604187209249994 5.4552918376559705E-5 40-41 7.50102627677696E-4 0.0 0.0 0.046097216028192944 5.4552918376559705E-5 42-43 8.182937756483955E-4 0.0 0.0 0.061385671403223804 5.4552918376559705E-5 44-45 8.728466940249553E-4 0.0 0.0 0.07832435255914559 5.4552918376559705E-5 46-47 0.0010228672195604944 0.0 0.0 0.09899990862386171 5.4552918376559705E-5 48-49 0.001091058367531194 0.0 0.0 0.12192577257161094 5.4552918376559705E-5 50-51 0.001091058367531194 0.0 0.0 0.1493795287446146 5.4552918376559705E-5 52-53 0.001091058367531194 0.0 0.0 0.1763968615706058 6.819114797069963E-5 54-55 0.0011183348267194738 0.0 0.0 0.20530990831018245 8.182937756483955E-5 56-57 0.0011456112859077538 2.7276459188279852E-5 0.0 0.2401692231528041 9.546760715897948E-5 58-59 0.0011456112859077538 2.7276459188279852E-5 0.0 0.28092025318009417 1.0910583675311941E-4 60-61 0.0011865259746901735 2.7276459188279852E-5 0.0 0.32688108691234574 1.0910583675311941E-4 62-63 0.0012274406634725933 2.7276459188279852E-5 0.0 0.377588024543358 1.0910583675311941E-4 64-65 0.0012274406634725933 2.7276459188279852E-5 0.0 0.4268902245261738 1.0910583675311941E-4 66-67 0.001281993581849153 2.7276459188279852E-5 0.0 0.4849618061380216 1.0910583675311941E-4 68-69 0.001281993581849153 2.7276459188279852E-5 0.0 0.5548577328079887 1.0910583675311941E-4 70-71 0.0013638229594139925 2.7276459188279852E-5 0.0 0.6364143457809455 1.0910583675311941E-4 72-73 0.001445652336978832 2.7276459188279852E-5 0.0 0.7214350690708138 1.0910583675311941E-4 74-75 0.001486567025761252 2.7276459188279852E-5 0.0 0.8061830277687994 1.0910583675311941E-4 76-77 0.001500205255355392 2.7276459188279852E-5 0.0 0.9034645194637994 1.0910583675311941E-4 78-79 0.0015274817145436716 2.7276459188279852E-5 0.0 1.0180392862841687 1.0910583675311941E-4 80-81 0.0015820346329202314 2.7276459188279852E-5 0.0 1.1487344404848119 1.3638229594139926E-4 82-83 0.0015820346329202314 2.7276459188279852E-5 0.0 1.2920858717488166 1.3638229594139926E-4 84-85 0.0016502257808909309 2.7276459188279852E-5 0.0 1.4332961009665413 1.3638229594139926E-4 86-87 0.0017320551584557706 2.7276459188279852E-5 0.0 1.5831256912877625 1.3638229594139926E-4 88-89 0.0017456933880499105 2.7276459188279852E-5 0.0 1.7571495009089881 1.636587551296791E-4 90-91 0.0018411609952088898 2.7276459188279852E-5 0.0 1.955394806289406 1.636587551296791E-4 92-93 0.0018547992248030298 2.7276459188279852E-5 0.0 2.174779367540741 1.636587551296791E-4 94-95 0.0018547992248030298 2.7276459188279852E-5 0.0 2.39869182101733 1.636587551296791E-4 96-97 0.0018547992248030298 2.7276459188279852E-5 0.0 2.630569000576897 1.636587551296791E-4 98-99 0.0021139255870916886 2.7276459188279852E-5 0.0 2.8849219825076067 1.636587551296791E-4 100-101 0.0022230314238448077 2.7276459188279852E-5 0.0 3.1800941856135774 1.636587551296791E-4 102-103 0.0023184990310037876 2.7276459188279852E-5 0.0 3.5048204322500487 1.636587551296791E-4 104-105 0.002591263622886586 2.7276459188279852E-5 0.0 3.8350019707241767 1.636587551296791E-4 106-107 0.002618540082074866 2.7276459188279852E-5 0.0 4.163901515616455 1.636587551296791E-4 108-109 0.0026458165412631455 2.7276459188279852E-5 0.0 4.515413245175817 1.636587551296791E-4 110-111 0.0027140076892338454 2.7276459188279852E-5 0.0 4.90981720680875 1.636587551296791E-4 112-113 0.002768560607610405 2.7276459188279852E-5 0.0 5.344099351774947 1.636587551296791E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10810 0.0 38.690517 1 GTACATG 17130 0.0 34.43269 1 ATCAACG 12475 0.0 33.144093 3 TCAACGC 12450 0.0 32.971375 4 TACATGG 17450 0.0 32.63996 2 CAACGCA 12720 0.0 32.365185 5 AACGCAG 12720 0.0 31.445137 6 TATCAAC 13270 0.0 31.293135 2 ACATGGG 18230 0.0 30.361012 3 GGTATCA 7145 0.0 27.684008 1 ACGCAGA 14840 0.0 26.54717 7 CGCAGAG 14675 0.0 24.97581 8 CGCATAG 345 1.8189894E-12 24.14493 8 AGTACAT 12460 0.0 23.286173 2 TAGCCCT 3035 0.0 23.163837 4 CATGGGA 10835 0.0 22.599566 4 GTGTAGC 2520 0.0 22.223907 1 CATGGGG 7910 0.0 21.993462 4 GAGTACA 14200 0.0 21.817657 1 CATGGGT 4235 0.0 21.52411 4 >>END_MODULE