FastQCFastQC Report
Thu 26 May 2016
SRR1364600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666165
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104920.2861846098034322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83030.2264764406402876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55300.15083881931118756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA158300.047.2323461
GGTATCA96400.045.9185831
ATCAACG197600.037.367363
TATCAAC201150.036.852842
TCAACGC199850.036.771544
CAACGCA200100.035.991225
AACGCAG206150.035.2138256
GTACATG180700.030.3653431
ACGCAGA245650.029.1393977
TACATGG184750.028.9087732
CGCAGAG248750.028.6565518
ACATGGG190150.026.9097523
GCAGAGT269100.025.4497139
GAGTACT144750.024.52033812-13
GTACTTT151250.023.36813714-15
AGTACTT151000.023.30823112-13
AGAGTAC247550.021.85496110-11
AGTACAT137250.021.6042232
TAGGACG9100.021.5785184
GAGTACA154500.020.9000591
Produced by FastQC (version 0.11.5)