##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364600_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3666165 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.55668661939656 31.0 30.0 33.0 26.0 34.0 2 29.729251138451215 31.0 30.0 34.0 26.0 34.0 3 29.750289198658542 31.0 30.0 34.0 26.0 34.0 4 33.40038350701619 35.0 33.0 37.0 28.0 37.0 5 32.798767649573875 35.0 33.0 37.0 26.0 37.0 6 32.785107871576976 35.0 33.0 37.0 26.0 37.0 7 32.57846687205841 35.0 33.0 37.0 25.0 37.0 8 32.5057159184052 35.0 33.0 37.0 24.0 37.0 9 33.81169969163963 38.0 33.0 39.0 23.0 39.0 10-11 33.60361167596112 37.0 33.0 39.0 22.0 39.0 12-13 33.46775595206435 37.0 33.0 39.0 20.0 39.0 14-15 34.4419773796324 38.0 33.0 41.0 19.0 41.0 16-17 34.35913236310968 38.0 33.0 41.0 18.5 41.0 18-19 34.403412830573636 38.5 33.0 41.0 19.0 41.0 20-21 34.42687344950377 39.0 33.0 41.0 19.0 41.0 22-23 34.41171933069025 39.0 33.0 41.0 19.0 41.0 24-25 34.40581956895012 39.0 33.0 41.0 19.5 41.0 26-27 34.30234986695908 39.0 33.0 41.0 19.0 41.0 28-29 34.071037446487 38.5 33.0 41.0 18.0 41.0 30-31 34.00835327924411 38.0 33.0 41.0 18.0 41.0 32-33 33.82875702539302 38.0 33.0 41.0 16.5 41.0 34-35 33.69700763604475 38.0 33.0 40.5 16.0 41.0 36-37 33.5330253821091 38.0 33.0 40.0 15.5 41.0 38-39 33.39182551794586 38.0 32.5 40.0 14.5 41.0 40-41 33.206276995170704 38.0 32.0 40.0 9.0 41.0 42-43 33.02983717317687 38.0 32.0 40.0 9.0 41.0 44-45 32.81813666324347 38.0 31.5 40.0 7.5 41.0 46-47 32.54956241740347 38.0 31.0 40.0 2.0 41.0 48-49 32.28919347601649 38.0 31.0 40.0 2.0 41.0 50-51 32.08932931278325 38.0 30.5 40.0 2.0 41.0 52-53 31.92720267636618 37.5 30.0 40.0 2.0 41.0 54-55 31.71397795789333 37.0 30.0 40.0 2.0 41.0 56-57 31.4853421763614 37.0 30.0 40.0 2.0 41.0 58-59 31.304263174188833 37.0 29.5 40.0 2.0 41.0 60-61 31.097076099957313 36.0 29.0 40.0 2.0 41.0 62-63 30.827995057505596 36.0 29.0 39.5 2.0 41.0 64-65 30.569939978151556 35.5 29.0 39.0 2.0 41.0 66-67 30.293021999828156 35.0 29.0 39.0 2.0 41.0 68-69 29.981353948881186 35.0 28.0 39.0 2.0 40.0 70-71 29.7171476188333 35.0 28.0 38.0 2.0 40.0 72-73 29.561456726579408 35.0 28.0 37.5 2.0 40.0 74-75 29.27897421419931 35.0 28.0 37.0 2.0 39.0 76-77 28.97748014614727 34.0 28.0 36.5 2.0 39.0 78-79 28.670150688798785 34.0 28.0 36.0 2.0 39.0 80-81 28.39150747443173 34.0 27.5 36.0 2.0 37.5 82-83 28.130396067825643 34.0 27.0 35.0 2.0 37.0 84-85 28.10909901763832 34.0 28.0 35.0 2.0 37.0 86-87 27.961924517854488 34.0 28.0 35.0 2.0 36.0 88-89 27.812681916935 34.0 27.0 35.0 2.0 36.0 90-91 27.65805903444062 34.0 27.0 35.0 2.0 36.0 92-93 27.530665286477834 34.0 27.0 35.0 2.0 35.0 94-95 27.444169861421948 34.0 27.0 35.0 2.0 35.0 96-97 27.356054896601762 34.0 27.0 35.0 2.0 35.0 98-99 27.26815896174886 34.0 26.0 35.0 2.0 35.0 100-101 27.170618070926977 34.0 26.0 35.0 2.0 35.0 102-103 26.002315907767382 32.0 24.5 34.0 2.0 35.0 104-105 26.923758068717582 33.5 25.5 35.0 2.0 35.0 106-107 27.091428372700083 34.0 26.0 35.0 2.0 35.0 108-109 27.10023457754902 34.0 26.0 35.0 2.0 35.0 110-111 27.049003113607817 34.0 26.0 35.0 2.0 35.0 112-113 27.000110469659713 34.0 26.0 35.0 2.0 35.0 114-115 26.919494894528754 34.0 25.0 35.0 2.0 35.0 116-117 26.806593538479582 34.0 25.0 35.0 2.0 35.0 118-119 26.70187061957113 34.0 25.0 35.0 2.0 35.0 120-121 26.56601939629013 34.0 25.0 35.0 2.0 35.0 122-123 26.38437918096976 34.0 25.0 35.0 2.0 35.0 124-125 25.5843274920796 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8648713661517711 1101 2 0.8570622995165635 1101 3 0.8087505655938116 1101 4 0.8499819501232793 1101 5 1.0780919822429524 1101 6 1.1196632668238493 1101 7 1.0138636642597803 1101 8 1.1570864092237514 1101 9 1.2617999566046976 1101 10-11 1.3593844348544195 1101 12-13 1.3520593086798058 1101 14-15 1.4876663914382249 1101 16-17 1.5411296789338493 1101 18-19 1.4838703143995247 1101 20-21 1.575808084945244 1101 22-23 1.5509629679285695 1101 24-25 1.3907975317190946 1101 26-27 1.4357474623925057 1101 28-29 1.5263600015661822 1101 30-31 1.5548807675213823 1101 32-33 1.5415502546383735 1101 34-35 1.4602857351043923 1101 36-37 1.5717244021069448 1101 38-39 1.6098314114137438 1101 40-41 1.5823356067625198 1101 42-43 1.620765125809065 1101 44-45 1.6022872999282782 1101 46-47 1.5117124845255105 1101 48-49 1.5740839190828595 1101 50-51 1.5349145296114983 1101 52-53 1.5674945459050384 1101 54-55 1.5837394025161018 1101 56-57 1.6281197395799722 1101 58-59 1.6636959304023051 1101 60-61 1.5507065498568409 1101 62-63 1.6532051386844273 1101 64-65 1.6371718001478577 1101 66-67 1.5815257750517233 1101 68-69 1.5426844813733425 1101 70-71 1.5942769958882366 1101 72-73 1.598061203869083 1101 74-75 1.4824316527073442 1101 76-77 1.5210332171630085 1101 78-79 1.5208492227109645 1101 80-81 1.4267025817565724 1101 82-83 1.336323504496093 1101 84-85 1.425930197304119 1101 86-87 1.4033668836398476 1101 88-89 1.4056872972721521 1101 90-91 1.4409064122345043 1101 92-93 1.3969744405426532 1101 94-95 1.3348830676294057 1101 96-97 1.4240425556834992 1101 98-99 1.4775723947230865 1101 100-101 1.5362715117654915 1101 102-103 1.5359319533603148 1101 104-105 1.4588939344789367 1101 106-107 1.3883597404238728 1101 108-109 1.4135159669656083 1101 110-111 1.4723899574890922 1101 112-113 1.4461027074161485 1101 114-115 1.4306610178070187 1101 116-117 1.4939698970740665 1101 118-119 1.4945756016521372 1101 120-121 1.4158751420108082 1101 122-123 1.5138924887422363 1101 124-125 1.3308142272240104 1102 1 0.43981115369857093 1102 2 -0.5173543766758044 1102 3 0.04684985748333048 1102 4 0.3059459409705809 1102 5 0.7737785445881613 1102 6 0.6150014893482236 1102 7 0.5640581890877598 1102 8 0.5795791947591269 1102 9 0.8413165924883117 1102 10-11 0.9448567391313603 1102 12-13 0.9882867503173429 1102 14-15 1.0084472582167976 1102 16-17 1.0949136881908217 1102 18-19 1.0268293177906713 1102 20-21 0.9859004641844251 1102 22-23 1.113258541385754 1102 24-25 1.142400907627291 1102 26-27 1.1014393491028542 1102 28-29 1.1750676648416984 1102 30-31 1.0574100415025853 1102 32-33 0.9887831389602724 1102 34-35 0.8948020100140965 1102 36-37 0.899365076646859 1102 38-39 0.8172797570502652 1102 40-41 0.7421526431266372 1102 42-43 0.563726485557666 1102 44-45 0.4240910765175201 1102 46-47 0.6429539239290207 1102 48-49 0.6244279974061726 1102 50-51 0.5801183896258237 1102 52-53 0.6266020178643821 1102 54-55 0.6086690627393878 1102 56-57 0.47245069449633803 1102 58-59 0.6512968276230069 1102 60-61 0.542404400658306 1102 62-63 0.6846672233889564 1102 64-65 0.6622205817606464 1102 66-67 0.5616148052552141 1102 68-69 0.4194707507280633 1102 70-71 0.373255630243726 1102 72-73 0.42293531421880814 1102 74-75 0.5156017456180209 1102 76-77 0.49515098690577375 1102 78-79 0.4879948816989135 1102 80-81 0.4504409278142383 1102 82-83 0.3928393342046448 1102 84-85 0.5046379006878468 1102 86-87 0.49347374194355 1102 88-89 0.5910660992799706 1102 90-91 0.531555232752261 1102 92-93 0.6229121705381822 1102 94-95 0.5487503816426162 1102 96-97 0.41402868826921235 1102 98-99 0.43941750282474246 1102 100-101 0.273355163457758 1102 102-103 0.32528041551119813 1102 104-105 0.3584289298965757 1102 106-107 0.6507196374259863 1102 108-109 0.6805058556853751 1102 110-111 0.5288406614884842 1102 112-113 0.48029290256265256 1102 114-115 0.639100838443099 1102 116-117 0.5922099487334442 1102 118-119 0.5126704716112869 1102 120-121 0.5739623613285261 1102 122-123 0.5516176211494717 1102 124-125 0.4381445544468576 1103 1 0.30829228544010334 1103 2 -0.9913436403249634 1103 3 0.04167238639360349 1103 4 0.2826303881016088 1103 5 0.5492490257498517 1103 6 0.5458078116239022 1103 7 0.5463572671175925 1103 8 0.6161972170160226 1103 9 0.7534328693734338 1103 10-11 0.8425424537671589 1103 12-13 0.8629968647603619 1103 14-15 0.9221598797597466 1103 16-17 1.0330825147890934 1103 18-19 1.0829976481424453 1103 20-21 1.1182997955790626 1103 22-23 0.9551546795124608 1103 24-25 0.8002345568157381 1103 26-27 0.8591812709722788 1103 28-29 0.9451334498369519 1103 30-31 0.8804497146742349 1103 32-33 0.8498673048168826 1103 34-35 0.8755418767831458 1103 36-37 1.0483990467277593 1103 38-39 1.1161680525762918 1103 40-41 1.099919632591778 1103 42-43 1.084168999246991 1103 44-45 1.1792783840433074 1103 46-47 1.0578074699471998 1103 48-49 1.0558533174136855 1103 50-51 1.1199652475482011 1103 52-53 1.0765846764658527 1103 54-55 1.0382807005025896 1103 56-57 1.1371464661222035 1103 58-59 1.0633724313555462 1103 60-61 1.0586808092767939 1103 62-63 1.0538722861923873 1103 64-65 1.1276091414068858 1103 66-67 1.122170917868452 1103 68-69 1.04438860457428 1103 70-71 1.0407059443289626 1103 72-73 1.043292601486307 1103 74-75 0.9635030790167001 1103 76-77 0.9197523900104123 1103 78-79 0.9610024928540462 1103 80-81 1.0609253263416996 1103 82-83 1.033160587441479 1103 84-85 1.0025362389087675 1103 86-87 1.005627866984053 1103 88-89 0.9232531134144892 1103 90-91 0.9925523172190012 1103 92-93 0.9670131849504351 1103 94-95 0.8811109969610413 1103 96-97 0.8922186206598823 1103 98-99 0.9340970970870721 1103 100-101 0.9098063577168176 1103 102-103 0.9719685147169308 1103 104-105 0.9959587625478257 1103 106-107 0.9338628450435422 1103 108-109 0.9244262744929124 1103 110-111 0.950534308580302 1103 112-113 0.9232719165773631 1103 114-115 0.878841724934027 1103 116-117 0.9629892157045283 1103 118-119 0.9540505372056813 1103 120-121 0.892377718285644 1103 122-123 0.9249657465766283 1103 124-125 0.8509314448260739 1104 1 0.3975930809630377 1104 2 -0.6033553753107093 1104 3 0.20920111965997634 1104 4 0.364433219307017 1104 5 0.7037705713175342 1104 6 0.6822815647318734 1104 7 0.8145247372569315 1104 8 0.6819492961210756 1104 9 0.7082086163082266 1104 10-11 0.8634305411604828 1104 12-13 0.7862716537425598 1104 14-15 0.792490474991709 1104 16-17 1.0226652948275614 1104 18-19 0.9035938348696391 1104 20-21 0.9204906009072644 1104 22-23 0.8042856436106192 1104 24-25 0.8165059075793693 1104 26-27 0.7420985221396492 1104 28-29 0.9585579325239593 1104 30-31 0.8423479766289859 1104 32-33 0.908492337417016 1104 34-35 0.9179062980730137 1104 36-37 0.9139600299584032 1104 38-39 0.8832873885852663 1104 40-41 0.9010601957361786 1104 42-43 0.9083789705323113 1104 44-45 0.8335764095508225 1104 46-47 0.96866285296624 1104 48-49 0.9073273248159737 1104 50-51 0.9479941983258584 1104 52-53 0.6507373485348076 1104 54-55 0.8199678145130207 1104 56-57 0.8451388370270081 1104 58-59 0.829435333811162 1104 60-61 0.9542208852649878 1104 62-63 0.8347282295884639 1104 64-65 0.8886052627213452 1104 66-67 0.8527748177473988 1104 68-69 0.9010125367373192 1104 70-71 0.9359951121610877 1104 72-73 0.897832780918506 1104 74-75 0.9919505796554269 1104 76-77 0.8176903684887478 1104 78-79 0.8250860791980585 1104 80-81 0.789831861426876 1104 82-83 0.812859475079776 1104 84-85 0.8497505010621751 1104 86-87 0.8397828542600436 1104 88-89 0.7881078157480452 1104 90-91 0.8321621666555146 1104 92-93 0.8350827517763229 1104 94-95 0.7874418167421915 1104 96-97 0.7778650540857655 1104 98-99 0.840410571074159 1104 100-101 0.8512012603307255 1104 102-103 0.8897230088876817 1104 104-105 0.8175117565547119 1104 106-107 0.8092559057167037 1104 108-109 0.7206314031645142 1104 110-111 0.672505779413342 1104 112-113 0.7511384476469516 1104 114-115 0.7828428468832271 1104 116-117 0.7586618533966032 1104 118-119 0.6589775826217164 1104 120-121 0.7629597860344326 1104 122-123 0.8208043694088794 1104 124-125 0.6778783168333007 1105 1 0.6605991783592735 1105 2 0.41778499333389263 1105 3 0.6652491736839821 1105 4 0.6005701996211315 1105 5 0.8509605111013343 1105 6 0.76567762627527 1105 7 0.8251962757444176 1105 8 0.8790598711173203 1105 9 0.9120593392080139 1105 10-11 1.0516280244758178 1105 12-13 1.0024201059413542 1105 14-15 1.0324033455798656 1105 16-17 1.1385986651748823 1105 18-19 1.1762773366656347 1105 20-21 1.1846183404072335 1105 22-23 0.9773011362502118 1105 24-25 1.0955100885186084 1105 26-27 1.140043526474372 1105 28-29 1.2472730734454132 1105 30-31 1.1554577222476183 1105 32-33 1.1955222175521243 1105 34-35 1.1944755171327728 1105 36-37 1.2784290484360312 1105 38-39 1.2553928838834096 1105 40-41 1.112818765638501 1105 42-43 1.092701335607778 1105 44-45 1.2202636358725911 1105 46-47 1.220830097570122 1105 48-49 1.211313948624003 1105 50-51 1.2524820456577004 1105 52-53 1.1626623534866134 1105 54-55 1.1166625656621676 1105 56-57 1.0760319542638754 1105 58-59 0.9736434788173227 1105 60-61 1.1303838243950963 1105 62-63 1.1644869475254183 1105 64-65 1.1097357037734277 1105 66-67 1.1602992454600596 1105 68-69 1.066137351833909 1105 70-71 1.0830314859968126 1105 72-73 1.051306758876052 1105 74-75 1.037010467337744 1105 76-77 0.9779389771470441 1105 78-79 1.0634875097945127 1105 80-81 0.9038670952680441 1105 82-83 0.9701623384308604 1105 84-85 0.8284978594992651 1105 86-87 0.8879396712148981 1105 88-89 0.9841542679286235 1105 90-91 0.9098666482841118 1105 92-93 0.7641954088493002 1105 94-95 0.7619899793942047 1105 96-97 0.8322408283300717 1105 98-99 0.9149420918198672 1105 100-101 0.9675827791500673 1105 102-103 0.583486786219936 1105 104-105 0.5115089362231942 1105 106-107 0.8379036445196668 1105 108-109 0.9077023685053867 1105 110-111 0.8838235706023383 1105 112-113 0.7145573634355458 1105 114-115 0.8923170473723161 1105 116-117 0.7303526598864067 1105 118-119 0.5946875485657586 1105 120-121 0.5516677490347277 1105 122-123 0.6509561944313056 1105 124-125 0.4860387818986496 1106 1 0.36362406115317825 1106 2 0.3372241239488183 1106 3 0.4267184851133088 1106 4 0.2922119605621205 1106 5 0.4813208187101736 1106 6 0.5218032621432727 1106 7 0.6201446675368203 1106 8 0.5832265996737078 1106 9 0.602240919733319 1106 10-11 0.724252807829231 1106 12-13 0.6123833082691448 1106 14-15 0.7437787279606027 1106 16-17 0.8456435392599246 1106 18-19 0.73562470372066 1106 20-21 0.826999768175682 1106 22-23 0.7708495435215639 1106 24-25 0.7507929818616503 1106 26-27 0.729137750248313 1106 28-29 0.891397758687333 1106 30-31 0.7740710746351738 1106 32-33 0.7391032635066139 1106 34-35 0.7189947903728822 1106 36-37 0.7850688119677969 1106 38-39 0.8541751287524946 1106 40-41 0.7789830441437928 1106 42-43 0.7200892950216726 1106 44-45 0.694749410488221 1106 46-47 0.5487452792092782 1106 48-49 0.6155923132031731 1106 50-51 0.6444322128381188 1106 52-53 0.7131534972633311 1106 54-55 0.704502056827053 1106 56-57 0.5032078990462594 1106 58-59 0.5180590587656724 1106 60-61 0.625901106480093 1106 62-63 0.682260840800442 1106 64-65 0.6011646683180452 1106 66-67 0.5614201399241452 1106 68-69 0.6333169083699701 1106 70-71 0.6857628160837379 1106 72-73 0.6450714023091884 1106 74-75 0.6466361035966237 1106 76-77 0.7261445531631274 1106 78-79 0.6008210818267159 1106 80-81 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-2.3169199747407987 2216 110-111 -2.252259646044564 2216 112-113 -2.2501997409103396 2216 114-115 -2.3747205091535264 2216 116-117 -2.5264386541460055 2216 118-119 -2.5499210022773866 2216 120-121 -2.5068337812787007 2216 122-123 -2.505895131892455 2216 124-125 -2.523917152996802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 145041.0 3 46031.0 4 21188.0 5 16219.0 6 17574.0 7 20674.0 8 23247.0 9 24711.0 10 26385.0 11 29568.0 12 34177.0 13 37913.0 14 40853.0 15 34575.0 16 22703.0 17 19485.0 18 17628.0 19 16296.0 20 15911.0 21 16649.0 22 19756.0 23 25833.0 24 35336.0 25 47196.0 26 57200.0 27 63431.0 28 72694.0 29 85264.0 30 99189.0 31 118537.0 32 142719.0 33 176829.0 34 226988.0 35 308938.0 36 485902.0 37 765293.0 38 307086.0 39 1146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.133974963960135 19.24722666333333 13.793566481204412 24.825231891502124 2 15.419602953351427 20.976694684507898 39.005469907320844 24.598232454819833 3 19.43944148722166 24.86538385479104 29.07026825033789 26.624906407649412 4 12.793537817092856 17.25644742520354 36.500520202232366 33.44949455547123 5 13.240647518328435 38.666874029691115 33.656464951556174 14.436013500424275 6 31.894592276082918 37.189636525560225 17.06661902352471 13.849152174832147 7 28.315290859874242 32.93060539572152 20.18513083429983 18.568972910104403 8 26.0099384533807 36.302958623782246 19.070123431466786 18.616979491370273 9 26.330408284005912 16.350699330550942 19.404639582297392 37.91425280314576 10-11 24.784637073878077 27.28651378504223 26.929293874886923 20.999555266192765 12-13 25.837880186758 24.344050781634145 27.756090155704864 22.061978875902987 14-15 22.550984598131993 25.727064926860123 26.096320767159593 25.62562970784829 16-17 21.91000311802155 28.923354663693747 26.652820922538655 22.51382129574605 18-19 21.771788706956148 27.2579793650549 29.358679020920942 21.61155290706801 20-21 22.826388511903478 26.343091791999353 29.314781013684136 21.515738682413033 22-23 22.246278633255763 26.39073767704548 29.26012964273161 22.102854046967153 24-25 21.958558875555244 26.437885365224968 29.595326451482677 22.008229307737103 26-27 21.839142537228955 26.76878972986759 29.377496648405078 22.014571084498378 28-29 21.666141773542 26.682368885427206 29.531051203551733 22.120438137479063 30-31 22.044070575110503 26.46012931769301 29.53619108796249 21.959609019233994 32-33 21.816353983382324 26.531568902819163 29.504916276747377 22.147160837051132 34-35 22.055417582132826 26.612522895177932 29.39472173238248 21.937337790306767 36-37 22.10644868024072 26.44437873841329 29.299804345744775 22.14936823560122 38-39 22.005992638083665 26.623078884883792 29.33411344006612 22.03681503696642 40-41 22.345898234258417 26.41285921391972 29.19809937632376 22.043143175498102 42-43 22.15405606373304 26.48975379987463 28.99913585278074 22.357054283611593 44-45 22.29716883991855 26.817369103681916 28.61813366283296 22.267328393566572 46-47 22.318676446380348 26.914574525583372 28.222345966577294 22.544403061458983 48-49 22.445892614844666 26.962541308565903 28.10084452158847 22.490721555000963 50-51 22.57095625805767 27.185375282704648 27.695610885480804 22.54805757375688 52-53 22.64555484606377 27.244930050807913 27.601706526254578 22.50780857687374 54-55 22.52921275081901 27.120468816933887 27.701689326815533 22.648629105431574 56-57 22.451362148964545 27.305511673779808 27.64210910202816 22.60101707522748 58-59 22.58251095934184 27.128351714714384 27.666189010340368 22.622948315603406 60-61 22.65123091841202 27.031734796442603 27.904090514202167 22.412943770943205 62-63 22.50370618889221 27.085128465303658 27.87846428079478 22.53270106500935 64-65 22.463173169637315 27.002669016091836 27.802323694234683 22.73183412003617 66-67 22.399919261680804 27.265194010635092 27.726411659049717 22.60847506863439 68-69 22.433517637003373 27.020424607178967 27.84019656633548 22.705861189482185 70-71 22.39987834699202 26.90588121374788 27.79936254914877 22.894877890111328 72-73 22.53871216360546 27.07772246716403 27.64371102682755 22.739854342402964 74-75 22.43974504821501 27.232440900249905 27.522768861599882 22.805045189935207 76-77 22.485089924157393 27.02356289781228 27.69380370934592 22.79754346868441 78-79 22.459684315527358 27.028822499153737 27.666620568997978 22.84487261632092 80-81 22.3038045051132 27.321833195539348 27.626189240285527 22.74817305906193 82-83 22.424154891390327 27.237043377314002 27.564170755417937 22.774630975877734 84-85 22.44951454589345 27.034004290598734 27.60364741807152 22.912833745436295 86-87 22.328802508137972 27.09204707068404 27.700424369351357 22.878726051826632 88-89 22.417655074895723 26.992886843032665 27.706821080562683 22.882637001508936 90-91 22.356045077076455 27.128197994361958 27.74820554994115 22.76755137862044 92-93 22.295860567518545 27.096906098357042 27.73838760529847 22.86884572882594 94-95 22.220626448812226 27.204090417339756 27.65164348294323 22.92363965090479 96-97 22.352622019307372 27.06229061278312 27.643252124202327 22.94183524370718 98-99 22.320109543604683 26.979940645696267 27.489784840648966 23.21016497005008 100-101 22.074936356958567 27.073393867301153 27.719534738203638 23.132135037536642 102-103 22.258134775133154 26.83949698301369 27.75620584871058 23.14616239314258 104-105 22.211479777450005 26.855720965565304 27.674456189305246 23.258343067679448 106-107 22.145970406682057 26.945620379070196 27.609150745233933 23.299258469013814 108-109 22.111921740244092 26.95956609284017 27.378421201297183 23.550090965618555 110-111 21.972020347895608 27.063767677537765 27.5757655521349 23.38844642243173 112-113 22.15060685454449 26.91413202547166 27.533847876520547 23.401413243463306 114-115 21.874613865728787 26.986915691051124 27.527701426047564 23.610769017172526 116-117 21.869991238588614 26.946522658013638 27.53484925026104 23.648636853136708 118-119 21.82495871256018 26.906399542943372 27.48800383189994 23.78063791259651 120-121 21.750494927420196 27.04816202356501 27.575634485085885 23.62570856392891 122-123 21.734139379900952 27.042456829065248 27.459709491550203 23.763694299483596 124-125 21.729511734905763 26.935899735208537 27.453139821212915 23.88144870867279 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 113.0 1 120.5 2 203.0 3 349.5 4 328.5 5 338.0 6 795.5 7 1687.0 8 2588.5 9 3017.0 10 2657.5 11 2166.0 12 2237.5 13 2506.0 14 2821.5 15 3247.5 16 3647.5 17 4082.5 18 4579.5 19 5083.0 20 5758.0 21 6545.0 22 7520.5 23 8963.5 24 10826.0 25 13392.5 26 17087.0 27 21628.5 28 26668.0 29 32625.5 30 40092.0 31 48162.0 32 56209.0 33 64828.5 34 73784.5 35 82048.0 36 90238.0 37 98324.5 38 103892.0 39 107477.0 40 112127.0 41 118822.5 42 124127.5 43 129483.5 44 135756.5 45 139521.5 46 141616.5 47 144895.5 48 150287.0 49 150212.0 50 144673.5 51 137599.5 52 130057.0 53 123975.0 54 118401.0 55 110587.0 56 99287.0 57 88481.5 58 79743.5 59 70082.5 60 58595.0 61 47220.5 62 37198.0 63 28392.5 64 21241.0 65 16118.5 66 12028.5 67 9200.5 68 6984.0 69 4907.5 70 3608.0 71 2931.0 72 2416.0 73 2046.5 74 1583.0 75 1185.0 76 840.0 77 498.0 78 318.5 79 190.0 80 107.5 81 77.5 82 52.5 83 29.0 84 19.5 85 16.5 86 13.5 87 10.5 88 5.5 89 4.5 90 3.5 91 2.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05910808706100244 2 0.07271904019595408 3 0.0 4 0.007719237950283198 5 0.02986772281116644 6 5.45529183765597E-4 7 2.7276459188279852E-5 8 2.7276459188279852E-5 9 4.3642334701247764E-4 10-11 0.059339936964102814 12-13 0.009587675404680368 14-15 0.0075964938839359385 16-17 0.054075580340764796 18-19 1.500205255355392E-4 20-21 0.010583266165052582 22-23 0.01296995634402707 24-25 0.0 26-27 0.0 28-29 0.05256173685581528 30-31 0.0 32-33 0.003505025005693961 34-35 0.0 36-37 1.0910583675311941E-4 38-39 0.0 40-41 0.0 42-43 0.0033550044801584217 44-45 0.0 46-47 1.2274406634725933E-4 48-49 1.7729698472381902E-4 50-51 4.2278511741833765E-4 52-53 1.0910583675311941E-4 54-55 1.3638229594139926E-5 56-57 0.0017593316176440503 58-59 1.3638229594139926E-5 60-61 0.0 62-63 0.0 64-65 5.45529183765597E-4 66-67 0.0 68-69 6.409967909245765E-4 70-71 0.0 72-73 0.002768560607610405 74-75 0.003777789597576759 76-77 5.45529183765597E-4 78-79 9.819525307780746E-4 80-81 0.0017456933880499105 82-83 2.4548813269451865E-4 84-85 2.727645918827985E-4 86-87 5.182527245773172E-4 88-89 2.7276459188279852E-5 90-91 0.0 92-93 0.007528302735965239 94-95 0.006300862072492646 96-97 0.007078241159358622 98-99 0.0012274406634725933 100-101 3.818704286359179E-4 102-103 1.7729698472381902E-4 104-105 6.137203317362966E-4 106-107 0.004596083373225156 108-109 0.005987182791827427 110-111 9.955907603722146E-4 112-113 0.003545939694476381 114-115 4.909762653890373E-4 116-117 0.002427604867756907 118-119 0.0026867312300455654 120-121 0.01298359457362121 122-123 0.0011183348267194738 124-125 0.001977543291150289 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3666165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.1988642518461 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88170764798927 39.30422935859961 2 13.120598359885333 13.96001862101069 3 5.154657278643553 8.226657384940465 4 2.534608295380315 5.393531305501617 5 1.4658060941047557 3.898960970990383 6 0.9333802007848068 2.9792859958147075 7 0.5988938191880225 2.2302329691777296 8 0.43850667702898655 1.8662445747834544 9 0.3058501694176277 1.4643793479827258 >10 1.4539493328403006 13.280142896692402 >50 0.07513862696017264 2.7713699740570577 >100 0.03488920424705137 3.142949702033081 >500 0.001497808016618428 0.5264761267753236 >1k 3.615398592928156E-4 0.2871563027495197 >5k 1.0329710265508982E-4 0.3800816319906323 >10k+ 5.164855132754491E-5 0.2882828369005793 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10492 0.2861846098034322 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8303 0.2264764406402876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5530 0.15083881931118756 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.182937756483955E-5 0.0 0.0 0.0 0.0 3 8.182937756483955E-5 0.0 0.0 0.0 0.0 4 8.182937756483955E-5 0.0 0.0 2.7276459188279852E-5 0.0 5 1.0910583675311941E-4 0.0 0.0 5.4552918376559705E-5 0.0 6 1.0910583675311941E-4 0.0 0.0 5.4552918376559705E-5 0.0 7 1.3638229594139926E-4 0.0 0.0 5.4552918376559705E-5 0.0 8 1.3638229594139926E-4 0.0 0.0 5.4552918376559705E-5 0.0 9 1.3638229594139926E-4 0.0 0.0 8.182937756483955E-5 0.0 10-11 1.3638229594139926E-4 0.0 0.0 8.182937756483955E-5 0.0 12-13 1.7729698472381902E-4 0.0 0.0 1.0910583675311941E-4 0.0 14-15 2.4548813269451865E-4 0.0 0.0 1.3638229594139926E-4 0.0 16-17 2.591263622886586E-4 0.0 0.0 1.7729698472381905E-4 0.0 18-19 3.2731751025935824E-4 0.0 0.0 2.3184990310037872E-4 0.0 20-21 3.818704286359179E-4 0.0 0.0 2.4548813269451865E-4 0.0 22-23 4.091468878241978E-4 0.0 0.0 3.1367928066521826E-4 0.0 24-25 4.6369980620075745E-4 0.0 0.0 5.182527245773172E-4 0.0 26-27 4.6369980620075745E-4 0.0 0.0 9.683143011839347E-4 0.0 28-29 5.046144949831773E-4 0.0 0.0 0.0016093110921085111 0.0 30-31 5.728056429538769E-4 0.0 0.0 0.0028640282147693844 0.0 32-33 5.864438725480168E-4 0.0 0.0 0.005932629873450867 0.0 34-35 6.273585613304365E-4 0.0 0.0 0.010528713246676022 0.0 36-37 6.819114797069962E-4 0.0 0.0 0.018534354018436158 0.0 38-39 7.637408572718358E-4 0.0 0.0 0.03154522505124564 0.0 40-41 7.637408572718358E-4 0.0 0.0 0.047311018462071405 0.0 42-43 8.182937756483955E-4 0.0 0.0 0.06251764445953742 1.3638229594139926E-5 44-45 8.728466940249553E-4 0.0 0.0 0.07961998437058888 2.7276459188279852E-5 46-47 9.819525307780746E-4 0.0 0.0 0.10030917866489915 2.7276459188279852E-5 48-49 0.0010092289899663546 0.0 0.0 0.12303046916873626 2.7276459188279852E-5 50-51 0.0010092289899663546 0.0 0.0 0.15030692835701612 2.7276459188279852E-5 52-53 0.0010092289899663546 0.0 0.0 0.17735153764219558 2.7276459188279852E-5 54-55 0.0010092289899663546 0.0 0.0 0.20622366969298983 2.7276459188279852E-5 56-57 0.0011183348267194738 0.0 0.0 0.24101479338764076 2.7276459188279852E-5 58-59 0.0011183348267194738 0.0 0.0 0.2815612499710188 2.7276459188279852E-5 60-61 0.0011183348267194738 0.0 0.0 0.3273584249481406 2.7276459188279852E-5 62-63 0.0011183348267194738 0.0 0.0 0.3784608712373829 2.7276459188279852E-5 64-65 0.0011728877450960335 0.0 0.0 0.427490306628316 2.7276459188279852E-5 66-67 0.0012001642042843135 0.0 0.0 0.48558916469935204 2.7276459188279852E-5 68-69 0.0012001642042843135 0.0 0.0 0.5556351118948547 2.7276459188279852E-5 70-71 0.001254717122660873 0.0 0.0 0.6362506870258158 2.7276459188279852E-5 72-73 0.001295631811443293 0.0 0.0 0.720193990177747 2.7276459188279852E-5 74-75 0.0013092700410374327 0.0 0.0 0.8047510136614147 2.7276459188279852E-5 76-77 0.0013092700410374327 0.0 0.0 0.9017870172237201 2.7276459188279852E-5 78-79 0.0013092700410374327 0.0 0.0 1.0159662753858596 2.7276459188279852E-5 80-81 0.001500205255355392 0.0 0.0 1.1462659209282724 2.7276459188279852E-5 82-83 0.0015547581737319514 0.0 0.0 1.2884035497583988 2.7276459188279852E-5 84-85 0.0016093110921085111 0.0 0.0 1.4287545705116926 2.7276459188279852E-5 86-87 0.0016638640104850709 0.0 0.0 1.5783386727002195 2.7276459188279852E-5 88-89 0.0016638640104850709 0.0 0.0 1.7513668915610725 2.7276459188279852E-5 90-91 0.0017593316176440503 0.0 0.0 1.9492166882832604 2.7276459188279852E-5 92-93 0.0018002463064264703 0.0 0.0 2.167387447100717 2.7276459188279852E-5 94-95 0.0018002463064264703 0.0 0.0 2.3905088832608463 2.7276459188279852E-5 96-97 0.0018002463064264703 0.0 0.0 2.622058745310154 2.7276459188279852E-5 98-99 0.0019502668319620095 0.0 0.0 2.8761389626489806 2.7276459188279852E-5 100-101 0.0021002873574975487 2.7276459188279852E-5 0.0 3.1715430156580515 2.7276459188279852E-5 102-103 0.002209393194250668 2.7276459188279852E-5 0.0 3.4968966208558534 2.7276459188279852E-5 104-105 0.002577625393292446 2.7276459188279852E-5 0.0 3.82638260962068 2.7276459188279852E-5 106-107 0.0027140076892338454 2.7276459188279852E-5 0.0 4.156414127569272 2.7276459188279852E-5 108-109 0.002782198837204545 2.7276459188279852E-5 0.0 4.508580492149154 2.7276459188279852E-5 110-111 0.002782198837204545 2.7276459188279852E-5 0.0 4.903680003491386 2.7276459188279852E-5 112-113 0.002782198837204545 2.7276459188279852E-5 0.0 5.340048797585488 2.7276459188279852E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15830 0.0 47.232346 1 GGTATCA 9640 0.0 45.918583 1 ATCAACG 19760 0.0 37.36736 3 TATCAAC 20115 0.0 36.85284 2 TCAACGC 19985 0.0 36.77154 4 CAACGCA 20010 0.0 35.99122 5 AACGCAG 20615 0.0 35.213825 6 GTACATG 18070 0.0 30.365343 1 ACGCAGA 24565 0.0 29.139397 7 TACATGG 18475 0.0 28.908773 2 CGCAGAG 24875 0.0 28.656551 8 ACATGGG 19015 0.0 26.909752 3 GCAGAGT 26910 0.0 25.449713 9 GAGTACT 14475 0.0 24.520338 12-13 GTACTTT 15125 0.0 23.368137 14-15 AGTACTT 15100 0.0 23.308231 12-13 AGAGTAC 24755 0.0 21.854961 10-11 AGTACAT 13725 0.0 21.604223 2 TAGGACG 910 0.0 21.578518 4 GAGTACA 15450 0.0 20.900059 1 >>END_MODULE