FastQCFastQC Report
Thu 26 May 2016
SRR1364599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264035
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67920.5373268936382299No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.10237058309303143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62400.032.74381
TACATGG64650.030.4985332
ACATGGG64950.029.9908053
CGCATAG1505.297894E-627.764668
TACCTGG11750.027.3763432
GTACCTG13600.026.2803941
GTATCAA59750.025.4227071
CATGGGG26500.023.8275574
CGCAGAT4250.023.7982798
TCGCGGA1751.8189894E-1223.7945182-83
CATGGGA39600.023.6169594
ATCAACG69850.021.8319783
CGCGGAA2051.8189894E-1221.76326882-83
GCGTAAA1653.1904643E-421.6614151
TCAACGC69350.021.6457984
CAACGCA70550.021.5309245
AACGCAG71000.021.0404936
TATCAAC71650.020.9509582
GAGTACA51550.020.6844441
ATGGGAT18450.020.6634645
Produced by FastQC (version 0.11.5)