##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364599_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1264035 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.829298239368374 30.0 26.0 31.0 7.0 34.0 2 25.69455671717951 30.0 25.0 31.0 7.0 34.0 3 26.21476541393237 31.0 26.0 31.0 7.0 34.0 4 29.566072933107073 35.0 28.0 37.0 7.0 37.0 5 29.829934297705364 35.0 30.0 37.0 7.0 37.0 6 29.866104182241788 35.0 30.0 37.0 7.0 37.0 7 29.741981828034824 35.0 30.0 37.0 2.0 37.0 8 29.37202213546302 35.0 29.0 37.0 2.0 37.0 9 30.400388438611273 37.0 28.0 39.0 2.0 39.0 10-11 30.354735826144054 37.0 28.5 39.0 2.0 39.0 12-13 30.353745347241176 37.0 29.0 39.0 2.0 39.0 14-15 30.944202889951622 37.0 27.5 40.0 2.0 41.0 16-17 31.33209286135273 37.5 29.5 40.0 2.0 41.0 18-19 31.14607625579988 37.0 28.5 40.0 2.0 41.0 20-21 31.04235839988608 37.0 27.5 40.0 2.0 41.0 22-23 31.086034010134213 37.0 28.0 40.0 2.0 41.0 24-25 31.16907917897843 37.0 29.0 40.0 2.0 41.0 26-27 30.975550518775194 37.0 28.5 40.0 2.0 41.0 28-29 30.87336505713845 37.0 28.0 40.0 2.0 41.0 30-31 30.73323483922518 37.0 28.0 40.0 2.0 41.0 32-33 30.62364966159956 37.0 28.0 40.0 2.0 41.0 34-35 30.667169421732783 37.0 28.0 40.0 2.0 41.0 36-37 30.59974526021827 37.0 28.0 40.0 2.0 41.0 38-39 30.298820048495493 37.0 27.0 40.0 2.0 41.0 40-41 30.17280573718291 37.0 27.0 40.0 2.0 41.0 42-43 29.677394217723403 36.0 25.5 40.0 2.0 41.0 44-45 29.551652446332575 36.0 25.5 40.0 2.0 41.0 46-47 29.507317439786082 36.0 26.0 40.0 2.0 41.0 48-49 29.428519779911156 36.0 26.0 40.0 2.0 41.0 50-51 29.207786967924147 35.0 25.5 40.0 2.0 41.0 52-53 29.11047360239234 35.0 25.5 40.0 2.0 41.0 54-55 28.93541041189524 35.0 25.5 40.0 2.0 41.0 56-57 28.716842492494273 35.0 24.5 39.0 2.0 41.0 58-59 28.508992235183364 35.0 24.0 39.0 2.0 40.5 60-61 28.217848793743848 34.0 23.0 39.0 2.0 40.0 62-63 28.03327835067858 34.0 23.0 39.0 2.0 40.0 64-65 27.817978536986715 34.0 23.0 38.5 2.0 40.0 66-67 27.54234811536073 34.0 22.0 38.0 2.0 40.0 68-69 27.294680527042367 34.0 22.0 37.5 2.0 40.0 70-71 26.859153425340278 33.0 21.0 37.0 2.0 40.0 72-73 26.62332213902305 33.0 20.5 36.5 2.0 39.0 74-75 26.588208791686938 33.0 21.0 36.0 2.0 39.0 76-77 26.340741356054224 33.0 20.0 36.0 2.0 39.0 78-79 26.04420644997963 33.0 20.0 35.5 2.0 38.0 80-81 25.644956033654132 32.5 18.0 35.0 2.0 37.0 82-83 25.74814265427777 33.0 20.0 35.0 2.0 37.0 84-85 25.581729936275497 33.0 18.5 35.0 2.0 36.5 86-87 25.457121044907776 33.0 18.5 35.0 2.0 36.0 88-89 25.28547627241334 33.0 18.0 35.0 2.0 36.0 90-91 25.148896984656282 33.0 17.5 35.0 2.0 35.5 92-93 25.022156427630563 33.0 15.5 35.0 2.0 35.0 94-95 24.917561618151396 33.0 12.0 35.0 2.0 35.0 96-97 24.80209606537794 32.5 10.5 35.0 2.0 35.0 98-99 24.70989846009011 32.0 7.5 35.0 2.0 35.0 100-101 24.60491837646901 32.0 7.0 35.0 2.0 35.0 102-103 23.51970752392141 30.5 6.0 34.0 2.0 35.0 104-105 24.31533739176526 32.0 2.0 34.5 2.0 35.0 106-107 24.467099012290006 32.0 2.0 35.0 2.0 35.0 108-109 24.433851910746142 32.0 2.0 35.0 2.0 35.0 110-111 24.36990194100638 32.0 2.0 35.0 2.0 35.0 112-113 24.274126903131638 32.0 2.0 35.0 2.0 35.0 114-115 24.17712919341632 32.0 2.0 35.0 2.0 35.0 116-117 24.065915105198826 32.0 2.0 35.0 2.0 35.0 118-119 23.940330766157583 32.0 2.0 35.0 2.0 35.0 120-121 23.75277029512632 32.0 2.0 35.0 2.0 35.0 122-123 23.548504194899664 32.0 2.0 35.0 2.0 35.0 124-125 22.654074847611025 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.017654152335684 1101 2 1.3816719995130917 1101 3 0.8267196362694342 1101 4 0.9623614573623946 1101 5 0.8470028571898673 1101 6 0.6860426637521293 1101 7 0.6700053848818506 1101 8 0.8607086830065711 1101 9 1.316789485136841 1101 10-11 1.3398491797891587 1101 12-13 1.3851760600445324 1101 14-15 1.7014514136171321 1101 16-17 1.2002911285210693 1101 18-19 1.2940269829454216 1101 20-21 1.3390888325110701 1101 22-23 1.1098036466642256 1101 24-25 1.1016820572698087 1101 26-27 1.4172078822099827 1101 28-29 1.3731500147395472 1101 30-31 1.391060130752308 1101 32-33 1.4006073330769908 1101 34-35 1.2079146751244352 1101 36-37 1.2020694776349465 1101 38-39 1.2474109204551596 1101 40-41 1.2794296682724706 1101 42-43 1.4023799661220835 1101 44-45 1.2483432813664734 1101 46-47 0.9906139860504162 1101 48-49 0.9135210120257895 1101 50-51 0.8209925421225925 1101 52-53 0.8226087657467964 1101 54-55 0.780935956792014 1101 56-57 0.7379000399124287 1101 58-59 0.633713364051296 1101 60-61 0.6516067497196154 1101 62-63 0.474499940048446 1101 64-65 0.5274912613531342 1101 66-67 0.4728288458411072 1101 68-69 0.24748357963433065 1101 70-71 0.16371480969353058 1101 72-73 0.1154202651139613 1101 74-75 0.10175942755732947 1101 76-77 0.26569925995918453 1101 78-79 0.36852548895554094 1101 80-81 0.5450405953140987 1101 82-83 0.5302989943211642 1101 84-85 0.4684225228761463 1101 86-87 0.5295204961729958 1101 88-89 0.43454311277019286 1101 90-91 0.5783790512466958 1101 92-93 0.5400888522979059 1101 94-95 0.5948482782383167 1101 96-97 0.5063444881758379 1101 98-99 0.5881337229981227 1101 100-101 0.5683258011156376 1101 102-103 0.4034344816562694 1101 104-105 0.5731999231025355 1101 106-107 0.5784527142876605 1101 108-109 0.5709787741047272 1101 110-111 0.6560690992640978 1101 112-113 0.5994274096781851 1101 114-115 0.6458553983558986 1101 116-117 0.5955491749182649 1101 118-119 0.5908822596837453 1101 120-121 0.567804744033598 1101 122-123 0.462514039154744 1101 124-125 0.34436363732312003 1102 1 0.4846994266803506 1102 2 0.9937953693824575 1102 3 0.7178328250677133 1102 4 0.545105628410429 1102 5 0.42588748971005685 1102 6 0.21672613488683723 1102 7 0.2174526777092325 1102 8 -0.005596902388933955 1102 9 0.806068498168937 1102 10-11 0.6257015658649543 1102 12-13 0.8839089847329085 1102 14-15 1.0355466329074439 1102 16-17 0.7883641196662232 1102 18-19 0.5961959876627283 1102 20-21 0.9539137395115738 1102 22-23 0.9284857429082578 1102 24-25 0.6075970797404331 1102 26-27 1.0872464244566977 1102 28-29 0.7020714946656135 1102 30-31 0.7611740738386601 1102 32-33 0.957915607004665 1102 34-35 0.6674999928805221 1102 36-37 0.5648633024851755 1102 38-39 0.8083305198350601 1102 40-41 0.7549459304939958 1102 42-43 1.0038605186625666 1102 44-45 0.6044078050197115 1102 46-47 0.5305354584252093 1102 48-49 0.46963240949477125 1102 50-51 0.5468931500558476 1102 52-53 0.4327843272047751 1102 54-55 0.46067214894333475 1102 56-57 0.3334332673228708 1102 58-59 0.4345305514153921 1102 60-61 0.2808825520631366 1102 62-63 0.09645604367090499 1102 64-65 0.18626820156133306 1102 66-67 0.257695651208774 1102 68-69 0.17515414956231368 1102 70-71 0.3542621845500875 1102 72-73 -0.05636331993306953 1102 74-75 0.17820597840241703 1102 76-77 -0.038092708676749965 1102 78-79 -0.06993906356033364 1102 80-81 0.14539195315848374 1102 82-83 -0.04594646897308863 1102 84-85 -0.0630181729371202 1102 86-87 0.12144211845450315 1102 88-89 -0.0765153041421911 1102 90-91 -0.02067278878829626 1102 92-93 -0.04800119912625078 1102 94-95 -0.12603199300333756 1102 96-97 -0.21062016935864136 1102 98-99 -0.13100667946224576 1102 100-101 -0.15743175218305083 1102 102-103 -0.19339812372521337 1102 104-105 -0.04875100650088271 1102 106-107 -0.09009529096019264 1102 108-109 -0.009570494163124721 1102 110-111 0.07246729960043652 1102 112-113 -0.16363482090574877 1102 114-115 -0.05102259855479474 1102 116-117 0.07741941219877901 1102 118-119 -0.04699150376066541 1102 120-121 -0.01608736004976663 1102 122-123 -0.08883712095821394 1102 124-125 -0.23627652587382997 1103 1 0.8789673635419817 1103 2 1.3464594930282878 1103 3 0.9834224588309617 1103 4 0.9183834915179716 1103 5 0.9624069294066331 1103 6 0.769312703927735 1103 7 0.6705929099898498 1103 8 0.9213110281127577 1103 9 1.5754876765190744 1103 10-11 1.53118203408658 1103 12-13 1.3893616854319042 1103 14-15 1.58279603244549 1103 16-17 1.3692542589356513 1103 18-19 1.3716236461170475 1103 20-21 1.571946300434977 1103 22-23 1.3799554913219154 1103 24-25 1.28513607080421 1103 26-27 1.6015388344860604 1103 28-29 1.479915728300579 1103 30-31 1.486763032716702 1103 32-33 1.5075854422955004 1103 34-35 1.3936960624749126 1103 36-37 1.3102278277163002 1103 38-39 1.3007243347981223 1103 40-41 1.459396970003091 1103 42-43 1.6998278793838502 1103 44-45 1.3645155693166267 1103 46-47 1.1732519457277881 1103 48-49 1.1491197026360211 1103 50-51 1.1978255068264758 1103 52-53 0.9654860899992102 1103 54-55 0.983267791758621 1103 56-57 0.997678306702138 1103 58-59 0.8934896668124672 1103 60-61 1.0796495139158218 1103 62-63 1.0629632925385408 1103 64-65 0.7809035505041493 1103 66-67 0.7044293355014766 1103 68-69 0.8192287003561525 1103 70-71 0.8087127236146472 1103 72-73 0.769120088042758 1103 74-75 0.846830908375928 1103 76-77 0.73771171302241 1103 78-79 0.7873159952905162 1103 80-81 0.9541377790935783 1103 82-83 0.9368644050289916 1103 84-85 1.0946304354981997 1103 86-87 1.041499106218776 1103 88-89 1.005960100438621 1103 90-91 1.070954602514437 1103 92-93 1.0474135564380518 1103 94-95 1.0480743774840455 1103 96-97 1.0651622327808603 1103 98-99 1.070802012887615 1103 100-101 0.8848846940544774 1103 102-103 0.8153187622171849 1103 104-105 1.0114789234568988 1103 106-107 1.0475802900058717 1103 108-109 1.0106697706892795 1103 110-111 0.8830729204219061 1103 112-113 1.0230116888081646 1103 114-115 1.052410708345537 1103 116-117 1.032283747403966 1103 118-119 1.0530173326600334 1103 120-121 0.9388180116075162 1103 122-123 0.7752023929986152 1103 124-125 0.6718254138205992 1104 1 0.548696002106972 1104 2 0.9871240971523285 1104 3 0.536837531901444 1104 4 0.6220337101355398 1104 5 0.18690649575723484 1104 6 0.3885805759977998 1104 7 -0.010101343651346184 1104 8 0.296447453990087 1104 9 0.7810755908111311 1104 10-11 0.7244420354760521 1104 12-13 0.8685213020275668 1104 14-15 1.0212186579628941 1104 16-17 0.7318043471222317 1104 18-19 0.6949395288905258 1104 20-21 0.9435140830858622 1104 22-23 0.8557550254009314 1104 24-25 0.7165016686240833 1104 26-27 1.0373272079758529 1104 28-29 0.9731077408596001 1104 30-31 1.0702039846835874 1104 32-33 1.1630577167473888 1104 34-35 0.9779066861008481 1104 36-37 0.969350124691065 1104 38-39 1.0532598391424592 1104 40-41 0.976381291392201 1104 42-43 1.2123270950050902 1104 44-45 1.0260669192324734 1104 46-47 0.9411338205274937 1104 48-49 0.7345811302501879 1104 50-51 0.683849418052727 1104 52-53 0.5514547025228254 1104 54-55 0.6706829107712906 1104 56-57 0.6319710940032728 1104 58-59 0.5207238856720693 1104 60-61 0.6640224759317235 1104 62-63 0.6428479272309389 1104 64-65 0.6087591654234821 1104 66-67 0.48764511473695293 1104 68-69 0.36086716673879593 1104 70-71 0.3736217468838028 1104 72-73 0.3040425914211866 1104 74-75 0.20025208839533093 1104 76-77 0.3293107643723232 1104 78-79 0.3367195312699671 1104 80-81 0.4518590187257736 1104 82-83 0.4908467520247086 1104 84-85 0.5511843962747811 1104 86-87 0.4891975708533778 1104 88-89 0.4278621685092503 1104 90-91 0.4979233371770313 1104 92-93 0.4436798855230393 1104 94-95 0.4224157794602341 1104 96-97 0.3726657400744635 1104 98-99 0.28528548266550047 1104 100-101 0.4037443810201218 1104 102-103 0.4131480220101551 1104 104-105 0.5047687032257571 1104 106-107 0.4739674638443745 1104 108-109 0.44721520975089746 1104 110-111 0.42871494004034005 1104 112-113 0.4729767279262518 1104 114-115 0.382965426607889 1104 116-117 0.36873419259493545 1104 118-119 0.4615559677961656 1104 120-121 0.4191876725093735 1104 122-123 0.4202886522514824 1104 124-125 0.3186287622214792 1105 1 0.9401081627507466 1105 2 1.2288540782115156 1105 3 0.67127021710942 1105 4 0.6778762017619862 1105 5 0.4739772509285025 1105 6 0.5664543360166334 1105 7 0.5851380382575009 1105 8 0.6114497547102644 1105 9 0.8717582091377025 1105 10-11 0.8408228956243669 1105 12-13 0.968838587391776 1105 14-15 1.368061588331333 1105 16-17 0.960504359963462 1105 18-19 1.0665010223254434 1105 20-21 0.9526284128272202 1105 22-23 1.0129435720948479 1105 24-25 0.7790246758258697 1105 26-27 1.1579129017767436 1105 28-29 1.289770974281037 1105 30-31 1.1279842980095722 1105 32-33 1.2430687388022008 1105 34-35 1.0807769682868553 1105 36-37 0.6275370769323949 1105 38-39 0.873331482498731 1105 40-41 0.9797347248157813 1105 42-43 1.3492631510356539 1105 44-45 1.0515571598985076 1105 46-47 0.47162344588463867 1105 48-49 0.6010537516800625 1105 50-51 0.803684435013853 1105 52-53 0.559344968871315 1105 54-55 0.6933015316233053 1105 56-57 0.7342917448395561 1105 58-59 0.7499707208441961 1105 60-61 0.6506669290558875 1105 62-63 0.7027253737525747 1105 64-65 0.4238552338672328 1105 66-67 0.41905569697967593 1105 68-69 0.39314653837466196 1105 70-71 0.6972587039405909 1105 72-73 0.6591049783916247 1105 74-75 0.6432132222758469 1105 76-77 0.5781048927204075 1105 78-79 0.6011942715026102 1105 80-81 0.7981512476420818 1105 82-83 0.7986871248483851 1105 84-85 0.5743172013342246 1105 86-87 0.7001118128463375 1105 88-89 0.8016185757474261 1105 90-91 0.6324726635475635 1105 92-93 0.7187996032300248 1105 94-95 0.6053711882928603 1105 96-97 0.6547991764943255 1105 98-99 0.7221305799913154 1105 100-101 0.7161919460020876 1105 102-103 0.5983748569682596 1105 104-105 0.7839138786690434 1105 106-107 0.7711744965574034 1105 108-109 0.8504752767393065 1105 110-111 0.8831359343794993 1105 112-113 0.8157802892270709 1105 114-115 0.8806041118800714 1105 116-117 0.8322360759751746 1105 118-119 0.8253228352853839 1105 120-121 0.699148533513771 1105 122-123 0.682666259288478 1105 124-125 0.7258753619710738 1106 1 0.17984526487786212 1106 2 -2.1140647034482036 1106 3 -0.4655829339369326 1106 4 0.2023457263089412 1106 5 0.5176578211488803 1106 6 0.33442558514652276 1106 7 0.16281961481047347 1106 8 0.3095744825963678 1106 9 0.6096703557806968 1106 10-11 0.8894756874151959 1106 12-13 0.9216649220208275 1106 14-15 0.873628354327721 1106 16-17 0.4442468743871295 1106 18-19 0.5008522897570309 1106 20-21 0.8792752396206929 1106 22-23 0.42805524633508085 1106 24-25 0.5257153797633443 1106 26-27 0.5441042457521768 1106 28-29 0.6883583283823675 1106 30-31 0.6979275418940176 1106 32-33 0.8178129191495138 1106 34-35 0.7226802925736244 1106 36-37 0.6220748548144535 1106 38-39 0.902950510006022 1106 40-41 1.0155475208822224 1106 42-43 1.1920167742240544 1106 44-45 0.9759006321630928 1106 46-47 0.8303825804386058 1106 48-49 0.5197813145041508 1106 50-51 0.5491550258562263 1106 52-53 0.5371571600953651 1106 54-55 0.5828213336938504 1106 56-57 0.5515399205358413 1106 58-59 0.512425259899544 1106 60-61 0.6512092015425957 1106 62-63 0.5904040564475608 1106 64-65 0.6305942730542426 1106 66-67 0.48887054221041737 1106 68-69 0.3177077017577119 1106 70-71 0.4971550725398899 1106 72-73 0.27719941201668874 1106 74-75 0.32421139471328075 1106 76-77 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127138.0 3 17056.0 4 10871.0 5 8545.0 6 7778.0 7 8498.0 8 9177.0 9 9673.0 10 10380.0 11 11242.0 12 12064.0 13 12529.0 14 12292.0 15 10460.0 16 8265.0 17 7303.0 18 6261.0 19 6043.0 20 6128.0 21 6264.0 22 7941.0 23 11110.0 24 16828.0 25 21296.0 26 23778.0 27 25428.0 28 29438.0 29 32397.0 30 37957.0 31 43574.0 32 53095.0 33 65293.0 34 84188.0 35 114548.0 36 160442.0 37 181472.0 38 47189.0 39 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59639303088154 19.691263866249752 14.165456309817525 22.546886793051176 2 13.315123641113615 21.343805492569796 41.71653314742951 23.624537718887083 3 18.88999806379325 27.21759154925107 27.665458389012503 26.226951997943175 4 11.21483685411088 17.897927204205097 38.007180402657944 32.88005553902608 5 12.730792339079313 40.032286918639024 33.30028977271374 13.936630969567911 6 30.262681051675806 38.36435943490863 17.889590693359025 13.48336882005654 7 27.67952222029279 34.61738215478204 20.310712221299795 17.392383403625374 8 24.627200527666833 37.921297096405425 19.26459606057387 18.186906315353866 9 25.349707589358268 18.972563264471294 19.81297134716599 35.86475779900445 10-11 23.48383263183956 29.462896756622925 26.994127259222576 20.05914335231493 12-13 24.60524449283873 26.609473181192477 27.29025555193655 21.49502677403224 14-15 21.616087157263138 27.824972109438008 25.411938151316456 25.1470025819824 16-17 21.4144213176289 31.15693684284762 25.72661668696547 21.702025152558004 18-19 21.382604925521132 29.698295780151597 27.806184524342658 21.112914769984613 20-21 22.43440017183125 28.501913683331182 27.77848669615591 21.285199448681663 22-23 22.04922756889727 28.35861066253794 27.58530751771633 22.006854250848455 24-25 22.013956102406052 28.51044805224145 27.718590248167494 21.757005597185 26-27 21.81248904577498 28.47773348063214 27.953080625475344 21.75669684811754 28-29 21.512880957390784 28.55957479942416 28.114339424213497 21.81320481897156 30-31 21.767805165616593 28.29944618168838 28.022319127550944 21.910429525144078 32-33 21.713394762009127 28.382656076171664 27.996629600462597 21.90731956135661 34-35 22.12102808384944 28.26965154380156 27.806453632074597 21.80286674027441 36-37 22.095783522014173 28.104914364675903 27.776888604353395 22.02241350895653 38-39 21.750754163951992 28.344239582392117 28.028505257351018 21.876500996304873 40-41 21.920882635189585 28.292470813287885 27.99121931148969 21.795427240032836 42-43 21.67100522808881 28.177974757148316 27.957364560927406 22.193655453835472 44-45 21.87510773031106 28.303501695749393 27.694109712746908 22.127280861192638 46-47 22.06549166289301 28.60384211733636 27.212957449396352 22.11770877037428 48-49 21.904394008728193 28.53536344024675 27.34582538465653 22.21441716636853 50-51 21.86924493554328 28.60744477515429 27.359833968866788 22.16347632043565 52-53 22.16911760334892 28.754209234576177 26.93295858909746 22.143714572977437 54-55 21.991729524709193 28.506115682044765 27.208682889562684 22.293471903683358 56-57 22.00370647301395 28.77426057765346 27.010139505874925 22.211893443457665 58-59 22.332683926263908 28.354501785407276 27.090495231651346 22.22231905667747 60-61 22.21777161824753 28.504573292731145 27.40520807828203 21.872447010739293 62-63 21.993465165640778 28.657997922607215 27.353487159700162 21.99504975205184 64-65 21.885866262722615 28.631000434947605 27.177451492250565 22.305681810079218 66-67 21.969060250049708 28.790689716085925 26.920822345568602 22.31942768829576 68-69 21.94403097102922 28.568588043959597 27.161163267294654 22.326217717716528 70-71 22.04549784583746 28.48410094559754 27.305559945608238 22.16484126295676 72-73 22.15540786436452 28.512390312362513 27.092485107350235 22.239716715922732 74-75 21.88824409488108 28.913915907843474 26.9981728049701 22.19966719230535 76-77 22.141849640600945 28.510978436056593 27.1400454165381 22.20712650680436 78-79 22.10190280469197 28.528206160149956 27.13271306136224 22.237177973795834 80-81 21.973431708588663 28.805900613736092 27.17881857783736 22.04184909983789 82-83 22.230695787559572 28.734485121959917 27.062265518839705 21.972553571640805 84-85 22.011294851719914 28.645564510858136 27.08942279164338 22.253717845778574 86-87 21.990161719460417 28.625671849957307 27.23919938664284 22.144967043939435 88-89 22.125417593772383 28.546615192489334 27.090068716758903 22.237898496979376 90-91 22.132449328934968 28.622323039008098 27.17295409248848 22.072273539568453 92-93 22.135816329683685 28.634190904113755 27.177250222045096 22.05274254415746 94-95 21.9901445351939 28.480597350848523 27.242482790976336 22.28677532298124 96-97 22.084523559545648 28.635990855893155 27.138742407974547 22.14074317658665 98-99 21.97997192726126 28.613275640144415 26.909419659712135 22.497332772882196 100-101 21.768482759986533 28.557423801318993 27.200483235299945 22.473610203394532 102-103 22.075569265883278 28.384499404630997 27.252050110059063 22.287881219426666 104-105 22.038701592313547 28.410490685429156 27.2744196068211 22.2763881154362 106-107 21.81194136858137 28.653501466577673 27.40921775262073 22.125339412220228 108-109 21.91484363517952 28.617200427362448 26.993199359352477 22.47475657810556 110-111 21.65427148575036 28.896402479933908 27.055977529460502 22.39334850485523 112-113 21.763688363653355 28.683092729451516 27.187077009346684 22.36614189754845 114-115 21.6232013533806 28.84317163729907 27.079294100502448 22.454332908817882 116-117 21.718916020455403 28.790785960018265 27.154892295482604 22.33540572404373 118-119 21.738159468683218 28.570195434644795 27.143388296728688 22.548256799943296 120-121 21.589382053034303 28.987847262090376 27.11188168283785 22.310889002037467 122-123 21.640786476850003 28.800019643307635 27.073982795472375 22.485211084369986 124-125 21.70782398218215 28.730216687761473 26.998964004707915 22.56299532534846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7165.0 1 4887.5 2 1968.5 3 1080.0 4 701.5 5 558.0 6 555.5 7 625.5 8 774.5 9 836.5 10 783.5 11 780.0 12 828.0 13 892.0 14 965.5 15 1045.0 16 1145.5 17 1230.5 18 1332.0 19 1530.5 20 1852.5 21 2303.5 22 2871.5 23 3583.0 24 4447.0 25 5639.5 26 7334.5 27 9224.5 28 10957.5 29 13046.0 30 15917.5 31 18855.5 32 21822.5 33 25083.5 34 28732.0 35 32000.5 36 35251.0 37 38210.0 38 39437.0 39 40137.0 40 40480.0 41 41093.5 42 41688.0 43 43058.5 44 45107.5 45 45856.5 46 45841.0 47 46580.0 48 48663.5 49 47971.5 50 45124.5 51 42303.5 52 39585.0 53 37535.0 54 36079.5 55 33985.0 56 30776.5 57 28346.5 58 26197.0 59 23452.5 60 19822.5 61 16146.0 62 12774.0 63 9817.5 64 7672.0 65 5927.5 66 4581.5 67 3606.0 68 2714.0 69 1936.5 70 1438.5 71 1174.0 72 974.5 73 778.5 74 577.5 75 460.0 76 346.5 77 211.5 78 143.5 79 108.5 80 79.0 81 45.5 82 34.0 83 31.5 84 23.0 85 14.5 86 12.0 87 11.0 88 8.0 89 5.5 90 4.0 91 4.5 92 5.5 93 4.0 94 1.0 95 0.5 96 1.0 97 1.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21352256859976187 2 0.25062597159097655 3 0.3037099447404542 4 0.2895489444516963 5 0.32293409597044387 6 0.2373352003702429 7 0.22689245155395224 8 0.20830119419161652 9 0.2075891885905058 10-11 0.14615892756134127 12-13 0.17230535546879636 14-15 0.19046149829711995 16-17 0.16265372398707315 18-19 0.19002638376310782 20-21 0.1844885624211355 22-23 0.2079056355243328 24-25 0.1984122275095231 26-27 0.24572104411665815 28-29 0.1764587214752756 30-31 0.15652256464417522 32-33 0.1948917553706978 34-35 0.15343720703936203 36-37 0.15339765117263368 38-39 0.18773214349286216 40-41 0.20810341485797465 42-43 0.27218391895794025 44-45 0.17432270467194344 46-47 0.15794657584639665 48-49 0.16079459825083958 50-51 0.16637197545954027 52-53 0.18785081109304727 54-55 0.17380847840447455 56-57 0.1731360286700922 58-59 0.14698960076263712 60-61 0.1609132658510247 62-63 0.14841361196485856 64-65 0.14350868449054022 66-67 0.1302179132698066 68-69 0.1340152764757305 70-71 0.13539973181122358 72-73 0.11198265870802629 74-75 0.11423734311154359 76-77 0.13603262567887756 78-79 0.14184733808794853 80-81 0.1526065338380662 82-83 0.1534767629060904 84-85 0.14070021795282567 86-87 0.1683102129292306 88-89 0.16162527145213543 90-91 0.15027273770109212 92-93 0.1506287405016475 94-95 0.17380847840447455 96-97 0.15980570158263022 98-99 0.15478210650812674 100-101 0.13528106421103847 102-103 0.17503471027305414 104-105 0.13116725407128757 106-107 0.13785219554838277 108-109 0.14829494436467344 110-111 0.12515476232857475 112-113 0.11455379004537058 114-115 0.1122595497751249 116-117 0.11736225658308512 118-119 0.10169813335864908 120-121 0.09430118628044319 122-123 0.12013116725407129 124-125 0.11716447724944325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1264035.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.96589967695988 #Duplication Level Percentage of deduplicated Percentage of total 1 86.03055957546698 60.19205500409842 2 8.451505624857658 11.826343893361061 3 2.378601780645836 4.9926304066831415 4 0.9972251893725486 2.7908703021990817 5 0.542244522811353 1.8969312941700054 6 0.33146391145358306 1.3914702465176463 7 0.22733352411875687 1.1133916179191878 8 0.16698768147841098 0.9346754695685306 9 0.12370976768125704 0.7789918675182158 >10 0.6872830164839344 8.718615573136578 >50 0.04415514315073121 2.1213682194404946 >100 0.017995441517081733 2.1705767342648548 >500 4.6741048094927284E-4 0.22393238751664113 >1k 3.505578607119546E-4 0.29285352759569494 >5k 1.1685262023731821E-4 0.5552934560105974 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6792 0.5373268936382299 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.10237058309303143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.1866760018512147E-4 14-15 0.0 0.0 0.0 3.955586672837382E-5 1.582234669134953E-4 16-17 3.955586672837382E-5 0.0 0.0 7.911173345674764E-5 1.582234669134953E-4 18-19 7.911173345674764E-5 0.0 0.0 7.911173345674764E-5 1.582234669134953E-4 20-21 7.911173345674764E-5 0.0 0.0 1.582234669134953E-4 1.582234669134953E-4 22-23 7.911173345674764E-5 0.0 0.0 3.560028005553644E-4 1.582234669134953E-4 24-25 7.911173345674764E-5 1.582234669134953E-4 0.0 4.7467040074048583E-4 1.582234669134953E-4 26-27 7.911173345674764E-5 1.582234669134953E-4 0.0 6.328938676539811E-4 1.582234669134953E-4 28-29 7.911173345674764E-5 1.582234669134953E-4 0.0 0.0011866760018512145 1.582234669134953E-4 30-31 7.911173345674764E-5 1.582234669134953E-4 0.0 0.002848022404442915 1.582234669134953E-4 32-33 7.911173345674764E-5 1.582234669134953E-4 0.0 0.0053795978750588395 1.977793336418691E-4 34-35 7.911173345674764E-5 1.582234669134953E-4 0.0 0.009256072814439475 2.3733520037024292E-4 36-37 7.911173345674764E-5 1.582234669134953E-4 0.0 0.014833450023140182 3.164469338269906E-4 38-39 7.911173345674764E-5 1.582234669134953E-4 0.0 0.02515753123924575 3.955586672837382E-4 40-41 7.911173345674764E-5 1.582234669134953E-4 0.0 0.0383691907265226 3.955586672837382E-4 42-43 7.911173345674764E-5 1.582234669134953E-4 0.0 0.051739073680712955 3.955586672837382E-4 44-45 7.911173345674764E-5 1.582234669134953E-4 0.0 0.06653296783712476 3.955586672837382E-4 46-47 1.582234669134953E-4 1.582234669134953E-4 0.0 0.08583623080057118 3.955586672837382E-4 48-49 1.582234669134953E-4 1.582234669134953E-4 0.0 0.10517904963074598 4.7467040074048583E-4 50-51 1.582234669134953E-4 1.582234669134953E-4 0.0 0.12911034900141216 5.537821341972335E-4 52-53 1.582234669134953E-4 1.582234669134953E-4 0.0 0.1529229807718932 5.537821341972335E-4 54-55 1.582234669134953E-4 1.582234669134953E-4 0.0 0.17578627174089323 5.537821341972335E-4 56-57 1.582234669134953E-4 1.582234669134953E-4 0.0 0.20703540645630858 5.537821341972335E-4 58-59 1.582234669134953E-4 1.582234669134953E-4 0.0 0.243268580379499 5.537821341972335E-4 60-61 1.582234669134953E-4 1.582234669134953E-4 0.0 0.2857515812457725 5.537821341972335E-4 62-63 1.582234669134953E-4 1.582234669134953E-4 0.0 0.3271665737103798 5.537821341972335E-4 64-65 1.582234669134953E-4 1.582234669134953E-4 0.0 0.37059891537813433 5.537821341972335E-4 66-67 1.582234669134953E-4 1.582234669134953E-4 0.0 0.4230895505266864 5.537821341972335E-4 68-69 1.582234669134953E-4 1.582234669134953E-4 0.0 0.4824233506192471 5.537821341972335E-4 70-71 1.582234669134953E-4 1.582234669134953E-4 0.0 0.5503012179251365 5.933380009256074E-4 72-73 1.582234669134953E-4 1.582234669134953E-4 0.0 0.6232026803055295 7.120056011107287E-4 74-75 1.582234669134953E-4 1.582234669134953E-4 0.0 0.6964997013532062 7.120056011107287E-4 76-77 1.582234669134953E-4 1.582234669134953E-4 0.0 0.7817425941528517 7.120056011107287E-4 78-79 1.582234669134953E-4 1.582234669134953E-4 0.0 0.8825704984434766 7.120056011107287E-4 80-81 2.3733520037024292E-4 1.582234669134953E-4 0.0 0.9958585007535392 7.120056011107287E-4 82-83 2.3733520037024292E-4 1.582234669134953E-4 0.0 1.1214879334828547 7.120056011107287E-4 84-85 2.3733520037024292E-4 1.582234669134953E-4 0.0 1.2429244443389622 7.120056011107287E-4 86-87 2.3733520037024292E-4 1.582234669134953E-4 0.0 1.372351240274201 7.515614678391025E-4 88-89 2.3733520037024292E-4 1.582234669134953E-4 0.0 1.5159785923649265 7.911173345674764E-4 90-91 2.3733520037024292E-4 1.582234669134953E-4 0.0 1.6822714560910101 7.911173345674764E-4 92-93 2.3733520037024292E-4 1.977793336418691E-4 0.0 1.8786663343973862 7.911173345674764E-4 94-95 2.3733520037024292E-4 2.3733520037024292E-4 0.0 2.0706705114969126 8.70229068024224E-4 96-97 2.3733520037024292E-4 2.3733520037024292E-4 0.0 2.263307582464093 0.0010680084016660932 98-99 2.3733520037024292E-4 2.3733520037024292E-4 0.0 2.479796841068483 0.001107564268394467 100-101 3.164469338269906E-4 2.3733520037024292E-4 0.0 2.734932181466494 0.001107564268394467 102-103 3.164469338269906E-4 2.3733520037024292E-4 0.0 3.0158975028381336 0.0011471201351228406 104-105 3.164469338269906E-4 2.3733520037024292E-4 0.0 3.2947268074064406 0.0011866760018512145 106-107 3.164469338269906E-4 2.3733520037024292E-4 0.0 3.570905868903947 0.0011866760018512145 108-109 3.164469338269906E-4 2.3733520037024292E-4 0.0 3.8636983944273693 0.0011866760018512145 110-111 3.164469338269906E-4 2.3733520037024292E-4 0.0 4.198697029749967 0.0011866760018512145 112-113 3.164469338269906E-4 2.3733520037024292E-4 0.0 4.579817805677849 0.0012262318685795884 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6240 0.0 32.7438 1 TACATGG 6465 0.0 30.498533 2 ACATGGG 6495 0.0 29.990805 3 CGCATAG 150 5.297894E-6 27.76466 8 TACCTGG 1175 0.0 27.376343 2 GTACCTG 1360 0.0 26.280394 1 GTATCAA 5975 0.0 25.422707 1 CATGGGG 2650 0.0 23.827557 4 CGCAGAT 425 0.0 23.798279 8 TCGCGGA 175 1.8189894E-12 23.79451 82-83 CATGGGA 3960 0.0 23.616959 4 ATCAACG 6985 0.0 21.831978 3 CGCGGAA 205 1.8189894E-12 21.763268 82-83 GCGTAAA 165 3.1904643E-4 21.661415 1 TCAACGC 6935 0.0 21.645798 4 CAACGCA 7055 0.0 21.530924 5 AACGCAG 7100 0.0 21.040493 6 TATCAAC 7165 0.0 20.950958 2 GAGTACA 5155 0.0 20.684444 1 ATGGGAT 1845 0.0 20.663464 5 >>END_MODULE