FastQCFastQC Report
Thu 26 May 2016
SRR1364599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264035
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61120.4835309148876416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53030.4195295225211327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37110.2935836428579905No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16870.13346149434153326No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.11668980684870277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG905.1330207E-639.692352
AACGCCG1555.0731614E-934.5817076
GTACATG60750.033.4411621
GTATCAA84700.032.4961471
TACATGG63650.031.0554432
GGTATCA61600.030.4651371
ACATGGG63050.029.539253
ATCAACG101050.027.204823
ACGCCGA2205.1004463E-927.0726137
TCAACGC101050.027.0292384
TATCAAC101950.026.9222762
GAGTACT59000.026.18996812-13
CAACGCA102350.026.019145
AACGCAG104650.025.496146
GTACTTT63250.024.57138614-15
AGTACTT61700.024.3200812-13
GCCGAGT2552.4527253E-823.3567629
CATGGGA40750.023.0720864
CGCAGAG115650.023.0719998
ACGCAGA115550.023.040427
Produced by FastQC (version 0.11.5)