FastQCFastQC Report
Thu 26 May 2016
SRR1364598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694094
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209481.236531148802841No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33330.19674232952834966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.1517035064169993No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22980.1356477267495192No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17540.10353616741455905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG114900.031.8380491
GTATCAA91700.031.6415541
TACATGG114900.030.956542
ATGGGAG26900.030.7874365
ACATGGG115450.028.7964133
ATCAACG107900.026.1178743
TATCAAC113450.025.7854732
TCAACGC109550.025.6157284
GTATATG11000.025.4567721
AACGCAG109450.025.4501936
CAACGCA111550.025.3708525
GAGTACT34600.025.27644212-13
CATGGGA83700.024.9138434
CATGGGG36800.023.3137874
GTACTTT37250.023.16121914-15
ACGCAGA123750.022.35977
CGCAGAG121200.021.6511678
CGCATAG2204.1769836E-621.6377778
CGCAGAT4700.021.5226848
AGTACTT38600.021.4240812-13
Produced by FastQC (version 0.11.5)