FastQCFastQC Report
Thu 26 May 2016
SRR1364598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694094
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176601.0424451063518316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149490.8824185670924989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107790.6362692979256169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48980.2891220912180788No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35740.21096822254255077No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.14184572992998026No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.13848109963201571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99500.045.4593121
GTATCAA149450.040.3143271
TATCAAC185400.032.220972
ATCAACG188100.031.8549963
TCAACGC191400.031.151254
GTACATG116950.030.7780361
CAACGCA193650.030.511765
AACGCAG195800.030.3189686
TACATGG118350.029.4901352
GAGTACT133600.028.50138912-13
GTACACG2751.8189894E-1228.1718141
ACATGGG119800.027.5162893
ATGGGAG27600.027.4073935
ACGCAGA221200.026.7029027
CGCAGAG221450.026.5920948
GTACTTT144650.026.3241314-15
AGTACTT137950.025.46608712-13
GCAGAGT227750.025.307489
CATGGGG37450.023.9924644
ACTTTTT164050.023.95660616-17
Produced by FastQC (version 0.11.5)