##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364598_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1694094 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.543613872665862 31.0 30.0 33.0 26.0 34.0 2 29.642015142016913 31.0 30.0 34.0 26.0 34.0 3 29.588943116497667 31.0 30.0 34.0 25.0 34.0 4 33.267832835722224 35.0 33.0 37.0 28.0 37.0 5 32.68083057964906 35.0 33.0 37.0 26.0 37.0 6 32.65287168244501 35.0 33.0 37.0 25.0 37.0 7 32.4584249752375 35.0 33.0 37.0 23.0 37.0 8 32.38181529478293 35.0 33.0 37.0 23.0 37.0 9 33.62934288179995 38.0 33.0 39.0 22.0 39.0 10-11 33.41977157111707 37.0 33.0 39.0 19.5 39.0 12-13 33.2727676268259 37.0 33.0 39.0 18.0 39.0 14-15 34.18683644473093 38.0 33.0 41.0 17.0 41.0 16-17 34.12695369914539 38.0 33.0 41.0 17.0 41.0 18-19 34.223569353294444 38.5 33.0 41.0 17.5 41.0 20-21 34.263013445534895 39.0 33.0 41.0 18.0 41.0 22-23 34.240090573486476 39.0 33.0 41.0 18.0 41.0 24-25 34.216057373439725 39.0 33.0 41.0 18.0 41.0 26-27 34.08714599071834 39.0 33.0 41.0 18.0 41.0 28-29 33.82168728535725 38.0 33.0 41.0 17.0 41.0 30-31 33.65690510680045 38.0 33.0 40.0 16.5 41.0 32-33 33.36058005045765 38.0 32.0 40.0 15.5 41.0 34-35 33.16873384829886 38.0 32.0 40.0 12.5 41.0 36-37 32.94395617952723 38.0 32.0 40.0 9.0 41.0 38-39 32.767556581866174 38.0 31.5 40.0 8.5 41.0 40-41 32.553239371605116 38.0 31.0 40.0 4.5 41.0 42-43 32.31657895016451 38.0 31.0 40.0 2.0 41.0 44-45 32.032873913726156 37.0 30.5 40.0 2.0 41.0 46-47 31.65447342355265 37.0 30.0 40.0 2.0 41.0 48-49 31.304183829232617 37.0 30.0 40.0 2.0 41.0 50-51 31.090529215025846 37.0 29.0 40.0 2.0 41.0 52-53 30.932556280820307 37.0 29.0 40.0 2.0 41.0 54-55 30.714090540430462 36.0 29.0 40.0 2.0 41.0 56-57 30.486967370169545 36.0 28.0 40.0 2.0 41.0 58-59 30.28966102235177 35.5 28.0 40.0 2.0 41.0 60-61 30.069656996601132 35.0 28.0 39.5 2.0 41.0 62-63 29.800606991111472 35.0 28.0 39.0 2.0 41.0 64-65 29.525322679851296 35.0 28.0 39.0 2.0 41.0 66-67 29.241240745790964 35.0 27.5 38.5 2.0 40.0 68-69 28.939374379461825 35.0 26.5 38.0 2.0 40.0 70-71 28.67010331185873 35.0 26.0 37.0 2.0 40.0 72-73 28.502219475424624 34.0 26.0 37.0 2.0 39.0 74-75 28.225763741563338 34.0 26.0 36.0 2.0 39.0 76-77 27.937232231505455 34.0 26.0 36.0 2.0 39.0 78-79 27.645179075069034 34.0 26.0 35.5 2.0 37.5 80-81 27.386817083349566 34.0 26.0 35.0 2.0 37.0 82-83 27.15436628664053 34.0 26.0 35.0 2.0 37.0 84-85 27.139561618186477 34.0 26.0 35.0 2.0 36.0 86-87 26.993366365738854 34.0 26.0 35.0 2.0 36.0 88-89 26.847659870113464 34.0 25.0 35.0 2.0 36.0 90-91 26.701547847994267 34.0 25.0 35.0 2.0 35.0 92-93 26.571990692370083 34.0 25.0 35.0 2.0 35.0 94-95 26.489948019413326 34.0 25.0 35.0 2.0 35.0 96-97 26.397963749355114 34.0 24.5 35.0 2.0 35.0 98-99 26.29744305805935 34.0 24.0 35.0 2.0 35.0 100-101 26.189724123927007 34.0 24.0 35.0 2.0 35.0 102-103 25.027334964883885 31.5 20.0 34.0 2.0 35.0 104-105 25.91965794105876 33.0 23.0 35.0 2.0 35.0 106-107 26.07615929222345 34.0 24.0 35.0 2.0 35.0 108-109 26.071024394159945 34.0 23.5 35.0 2.0 35.0 110-111 25.996138644018572 34.0 23.0 35.0 2.0 35.0 112-113 25.93218440062948 34.0 23.0 35.0 2.0 35.0 114-115 25.843299722447515 34.0 22.5 35.0 2.0 35.0 116-117 25.708297768600797 34.0 20.0 35.0 2.0 35.0 118-119 25.59989793954763 34.0 19.0 35.0 2.0 35.0 120-121 25.455091925241454 34.0 18.0 35.0 2.0 35.0 122-123 25.26043241992475 34.0 8.5 35.0 2.0 35.0 124-125 24.490105920922925 33.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7492320375675909 1101 2 0.8384028378082853 1101 3 0.7506444696262875 1101 4 0.7276272639266494 1101 5 1.0840555686797373 1101 6 1.114213071367125 1101 7 1.0154536925879114 1101 8 1.0008505623498039 1101 9 1.150228117876921 1101 10-11 1.213376315511674 1101 12-13 1.235801000638773 1101 14-15 1.3550164605878692 1101 16-17 1.3337323263681782 1101 18-19 1.1853052939061826 1101 20-21 1.3939890796037844 1101 22-23 1.2790888382560013 1101 24-25 1.2120218012990236 1101 26-27 1.3795434462110947 1101 28-29 1.3798177018438338 1101 30-31 1.4024830963296466 1101 32-33 1.48304482820447 1101 34-35 1.3810305134095486 1101 36-37 1.4320909776870678 1101 38-39 1.5609613212700424 1101 40-41 1.4340045360008773 1101 42-43 1.519352590158185 1101 44-45 1.3929732036908433 1101 46-47 1.560478629248081 1101 48-49 1.7026149105925512 1101 50-51 1.582913437190026 1101 52-53 1.5353839213112295 1101 54-55 1.4706465861516946 1101 56-57 1.4131260694138597 1101 58-59 1.4286532744725768 1101 60-61 1.296781248760901 1101 62-63 1.3472131247629342 1101 64-65 1.3953383570422808 1101 66-67 1.3577702914063963 1101 68-69 1.2839875892077295 1101 70-71 1.2448014728721901 1101 72-73 1.3180342570802743 1101 74-75 1.335369786851281 1101 76-77 1.3511240223908558 1101 78-79 1.3544546634452175 1101 80-81 1.3122856468416408 1101 82-83 1.192827778991571 1101 84-85 1.2704964921537858 1101 86-87 1.2471769496490488 1101 88-89 1.1776735096614104 1101 90-91 1.2651684527418716 1101 92-93 1.278699669785329 1101 94-95 1.1896227836444808 1101 96-97 1.328036750608085 1101 98-99 1.4380643131241513 1101 100-101 1.372430266482226 1101 102-103 1.4172590187247813 1101 104-105 1.391207220530955 1101 106-107 1.4877386080428536 1101 108-109 1.4171166840244886 1101 110-111 1.4807899150347872 1101 112-113 1.3968051234267946 1101 114-115 1.3165466704417987 1101 116-117 1.342474120945024 1101 118-119 1.389063132192497 1101 120-121 1.4235609904711701 1101 122-123 1.4513171549651638 1101 124-125 1.1637681691174748 1102 1 0.4129434127635072 1102 2 -0.48112501143390674 1102 3 0.15516890085048018 1102 4 0.3716836748316439 1102 5 0.6908543026522764 1102 6 0.43270622972782036 1102 7 0.5283329765171416 1102 8 0.49957108694752606 1102 9 0.8422888705452962 1102 10-11 0.792459566262913 1102 12-13 0.9380192703051833 1102 14-15 0.9488716326814739 1102 16-17 1.1696595377938053 1102 18-19 1.0968911742099152 1102 20-21 0.9799031632787774 1102 22-23 1.029316892899459 1102 24-25 1.007762566646889 1102 26-27 1.1439802096762506 1102 28-29 1.0722081691757452 1102 30-31 0.9584660686765147 1102 32-33 0.9111072362501815 1102 34-35 0.8549441520217869 1102 36-37 0.7201478464623889 1102 38-39 0.608624341517185 1102 40-41 0.6175404155841804 1102 42-43 0.5311672455307104 1102 44-45 0.34753901934840314 1102 46-47 0.30824739192556905 1102 48-49 0.5436694692039552 1102 50-51 0.5864831447453653 1102 52-53 0.5330286907831301 1102 54-55 0.4451580177008836 1102 56-57 0.323840795388076 1102 58-59 0.41962290294122795 1102 60-61 0.26796375491253244 1102 62-63 0.37012785361885037 1102 64-65 0.3763230504052437 1102 66-67 0.3098998687088361 1102 68-69 0.12408710396124079 1102 70-71 0.07821367943894586 1102 72-73 0.15189160645934408 1102 74-75 0.20941198136291916 1102 76-77 0.39101077152754726 1102 78-79 0.43572216377741313 1102 80-81 0.44347317996119173 1102 82-83 0.3038546892794045 1102 84-85 0.4737543355845446 1102 86-87 0.2985983488431323 1102 88-89 0.40199731043338716 1102 90-91 0.3687060613459927 1102 92-93 0.45796911046310385 1102 94-95 0.41387758367842054 1102 96-97 0.30182671620377377 1102 98-99 0.3583558132258098 1102 100-101 0.16843686878021913 1102 102-103 0.2605044708397095 1102 104-105 0.23278752954539428 1102 106-107 0.5373863200520255 1102 108-109 0.4891649813392185 1102 110-111 0.34148014654747527 1102 112-113 0.3427680999681435 1102 114-115 0.4409128962615263 1102 116-117 0.466046357993779 1102 118-119 0.4044829560565084 1102 120-121 0.4421331554773893 1102 122-123 0.40102538270282295 1102 124-125 0.27141716508469216 1103 1 0.37511996179805607 1103 2 -0.9074535157828727 1103 3 0.18434612708485076 1103 4 0.31720895295585194 1103 5 0.6831084495005726 1103 6 0.7582541131982268 1103 7 0.6973795015855444 1103 8 0.7530210438020575 1103 9 0.8838508487372252 1103 10-11 0.9596273020941553 1103 12-13 0.9670559335511655 1103 14-15 1.144874392710996 1103 16-17 1.1117512687517674 1103 18-19 1.127688877173746 1103 20-21 1.126822544481442 1103 22-23 1.0851267230231656 1103 24-25 1.0198355345271253 1103 26-27 0.8809246616807087 1103 28-29 1.0988390120253584 1103 30-31 0.9011018808600255 1103 32-33 0.7397535890568818 1103 34-35 0.9435798425334667 1103 36-37 1.0850112618228138 1103 38-39 1.086809782201371 1103 40-41 1.3075246622834271 1103 42-43 1.2502128900792542 1103 44-45 1.210652761701894 1103 46-47 1.2301708155148532 1103 48-49 1.1582186990692733 1103 50-51 1.1945945737330455 1103 52-53 1.0063783964493567 1103 54-55 1.04325905655422 1103 56-57 1.1487219652307488 1103 58-59 1.2682349635017793 1103 60-61 1.105976197458613 1103 62-63 1.1528563765387858 1103 64-65 1.1413920271291005 1103 66-67 1.0531136221088424 1103 68-69 1.0947610698707102 1103 70-71 1.1554171003386458 1103 72-73 0.9484604607108977 1103 74-75 0.9671772059515149 1103 76-77 0.8872329410964639 1103 78-79 0.8982589254815245 1103 80-81 0.937581621585128 1103 82-83 0.8786999179023844 1103 84-85 0.8902676050188205 1103 86-87 0.9241698462552037 1103 88-89 0.8895914259992175 1103 90-91 0.9163128884166944 1103 92-93 0.9481945395564431 1103 94-95 0.7778232572040693 1103 96-97 0.7761425122497556 1103 98-99 0.8504557890515372 1103 100-101 0.9318225316755928 1103 102-103 1.020850178945775 1103 104-105 0.9332356341063281 1103 106-107 0.873689673551933 1103 108-109 0.8948199200150135 1103 110-111 0.9146888889890121 1103 112-113 0.9623141999855953 1103 114-115 0.8861993934094414 1103 116-117 0.9857258967934861 1103 118-119 0.9666085149865005 1103 120-121 0.9655894040465469 1103 122-123 1.0024221273408536 1103 124-125 0.9891430704592246 1104 1 0.32476021844238545 1104 2 -0.7457633864285462 1104 3 0.09559326004680813 1104 4 0.3039100992766137 1104 5 0.6682873075556941 1104 6 0.536700600408345 1104 7 0.6972716496560452 1104 8 0.7564432566022532 1104 9 0.8553136608356766 1104 10-11 0.9220838600168193 1104 12-13 0.8713294658223774 1104 14-15 1.0092385391448673 1104 16-17 1.1041991614363056 1104 18-19 1.0016139889967235 1104 20-21 0.9343204477039606 1104 22-23 0.899447110622603 1104 24-25 0.9256138947945942 1104 26-27 0.8597890828434274 1104 28-29 1.0222578069392014 1104 30-31 0.9222448764489144 1104 32-33 1.0577132839564456 1104 34-35 1.0492523286718622 1104 36-37 1.015437341840915 1104 38-39 1.0709638924105889 1104 40-41 0.973389650209576 1104 42-43 1.0236881450146456 1104 44-45 1.0432739838713019 1104 46-47 0.9058192713780713 1104 48-49 0.8884303422567612 1104 50-51 0.8619699522191446 1104 52-53 0.6382808426794497 1104 54-55 0.8147960670717964 1104 56-57 0.8169357456788333 1104 58-59 0.8820040701355083 1104 60-61 0.8895354532864808 1104 62-63 0.8224994268262158 1104 64-65 0.7740903835151407 1104 66-67 0.7347833986606602 1104 68-69 0.7238749720153272 1104 70-71 0.8703690861429862 1104 72-73 0.9222631034231057 1104 74-75 0.939090122162618 1104 76-77 0.8265821458661762 1104 78-79 0.7704574793385746 1104 80-81 0.7971457390936685 1104 82-83 0.8621352212691562 1104 84-85 0.862485689655081 1104 86-87 0.873392292559469 1104 88-89 0.8957618542980761 1104 90-91 0.8960889519634208 1104 92-93 0.9636440966836624 1104 94-95 0.8449275534192395 1104 96-97 0.8218694126223873 1104 98-99 0.9265400717805825 1104 100-101 1.0132487297436512 1104 102-103 1.0358340258788523 1104 104-105 0.926185548164721 1104 106-107 0.8625605287466556 1104 108-109 0.8972757918881591 1104 110-111 0.8589263511249499 1104 112-113 0.8702200863523082 1104 114-115 0.9042536209887544 1104 116-117 0.8869220952138406 1104 118-119 0.7824233780330943 1104 120-121 0.8794096862663068 1104 122-123 0.9781939176025354 1104 124-125 0.9778671357785527 1105 1 0.7103404310962098 1105 2 0.5221250521945358 1105 3 0.677411089977344 1105 4 0.6919491403990179 1105 5 0.7740988132531612 1105 6 0.786212735253855 1105 7 0.7248615630402568 1105 8 0.6375661354979556 1105 9 0.916838265496672 1105 10-11 0.9812042735335282 1105 12-13 1.007424787855065 1105 14-15 1.1185675272973512 1105 16-17 1.0146475447967944 1105 18-19 1.1051119687556081 1105 20-21 1.1441146719283921 1105 22-23 0.9998858468478886 1105 24-25 1.022808186310769 1105 26-27 1.0292496472008352 1105 28-29 1.1871947879218325 1105 30-31 1.1674122274468317 1105 32-33 1.1516667037765416 1105 34-35 1.1608650676543348 1105 36-37 1.226562364544435 1105 38-39 1.211388515234404 1105 40-41 1.0416205025816154 1105 42-43 1.1797519947575381 1105 44-45 1.2124284601122568 1105 46-47 1.1635204543431357 1105 48-49 1.1714546595879654 1105 50-51 1.1505596839751924 1105 52-53 1.1629633655839555 1105 54-55 1.1461968966063196 1105 56-57 1.0246787626714884 1105 58-59 1.0253521619976738 1105 60-61 1.1770444974757162 1105 62-63 1.1860457613614699 1105 64-65 1.0900139627213967 1105 66-67 1.0691691828328054 1105 68-69 0.9379295676544679 1105 70-71 1.0106792236742095 1105 72-73 1.0520123076833272 1105 74-75 1.0630611688470104 1105 76-77 0.9788184053979307 1105 78-79 0.9500674849660697 1105 80-81 0.8571513665891963 1105 82-83 0.9238444316034986 1105 84-85 0.7324409674419563 1105 86-87 0.8037545663058587 1105 88-89 0.895235728129034 1105 90-91 0.8384690550448504 1105 92-93 0.6647341634096797 1105 94-95 0.9734677393945645 1105 96-97 0.9448403134087471 1105 98-99 0.9532057657936974 1105 100-101 0.9684461238261477 1105 102-103 0.6583368619579488 1105 104-105 0.542730533887557 1105 106-107 0.8909009237432457 1105 108-109 0.9559632753257539 1105 110-111 0.858641701086686 1105 112-113 0.6783217445281089 1105 114-115 1.008074065173826 1105 116-117 0.6795070510424672 1105 118-119 0.4992083511312835 1105 120-121 0.44912765643760366 1105 122-123 0.5319588312100585 1105 124-125 0.2684077326503811 1106 1 0.4633451660951273 1106 2 0.36490647420987443 1106 3 0.5378223309726131 1106 4 0.34945511197736323 1106 5 0.44453064690298305 1106 6 0.5489100086581473 1106 7 0.5336068101885445 1106 8 0.6383319840450312 1106 9 0.6340176110495719 1106 10-11 0.780651711256418 1106 12-13 0.7744264798629814 1106 14-15 0.8294556930062811 1106 16-17 0.9117396521117698 1106 18-19 0.7804361974054856 1106 20-21 0.8353549968055773 1106 22-23 0.8248367698739258 1106 24-25 0.7962698946121094 1106 26-27 0.7862807343851443 1106 28-29 0.9410845115605468 1106 30-31 0.7903303363037111 1106 32-33 0.7816792225948461 1106 34-35 0.7305492698966347 1106 36-37 0.827261465492164 1106 38-39 0.8953517486746563 1106 40-41 0.8454498910540948 1106 42-43 0.8501876904409471 1106 44-45 0.8066396361087698 1106 46-47 0.698941641896603 1106 48-49 0.7729569177845832 1106 50-51 0.9015848717210311 1106 52-53 0.983925609469189 1106 54-55 0.9679507845082433 1106 56-57 0.7141943616437487 1106 58-59 1.0295414482792928 1106 60-61 1.0624398895595704 1106 62-63 1.0687269453880965 1106 64-65 0.807228940999277 1106 66-67 0.791353286702563 1106 68-69 0.8892564312314093 1106 70-71 0.8950501566978062 1106 72-73 0.9029333662594468 1106 74-75 0.8420798333995201 1106 76-77 0.9832915875824426 1106 78-79 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32-33 -2.8319751904509047 2216 34-35 -2.311952768501911 2216 36-37 -2.29566922895712 2216 38-39 -2.3052557986266393 2216 40-41 -2.4545163795476697 2216 42-43 -2.6745425092175807 2216 44-45 -2.9390717127187145 2216 46-47 -2.5011577343882827 2216 48-49 -2.554481738614598 2216 50-51 -2.7097514117731656 2216 52-53 -2.4121337654219204 2216 54-55 -2.2186465185336743 2216 56-57 -2.251832817003592 2216 58-59 -2.2068137557347853 2216 60-61 -2.0963250151409447 2216 62-63 -2.099544283574584 2216 64-65 -2.172482407828209 2216 66-67 -2.129565003133319 2216 68-69 -2.1095754444437027 2216 70-71 -2.694123403356553 2216 72-73 -3.0803057013038853 2216 74-75 -2.7283615757088135 2216 76-77 -2.896237246174831 2216 78-79 -2.558894837882182 2216 80-81 -2.22244420893794 2216 82-83 -2.209884569433484 2216 84-85 -2.16821025965794 2216 86-87 -2.23194194250852 2216 88-89 -2.0982630620541656 2216 90-91 -2.0706474330298157 2216 92-93 -2.0913515607520914 2216 94-95 -1.9542308433914677 2216 96-97 -2.061532310165397 2216 98-99 -2.0718540337223743 2216 100-101 -2.1470519750331682 2216 102-103 -2.066167113154389 2216 104-105 -2.0406057951826106 2216 106-107 -2.2124112089909396 2216 108-109 -2.1733834236026937 2216 110-111 -2.063641741706263 2216 112-113 -2.054104110985204 2216 114-115 -2.16991210834847 2216 116-117 -2.2638832012907493 2216 118-119 -2.308197110327413 2216 120-121 -2.304378738308337 2216 122-123 -2.3407020744242324 2216 124-125 -2.429521060989437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69883.0 3 22995.0 4 10710.0 5 8820.0 6 10533.0 7 12582.0 8 13024.0 9 13657.0 10 14397.0 11 15953.0 12 18570.0 13 21353.0 14 24362.0 15 19615.0 16 12110.0 17 10236.0 18 9381.0 19 8883.0 20 8691.0 21 9016.0 22 10349.0 23 13610.0 24 18270.0 25 23597.0 26 27566.0 27 30093.0 28 34182.0 29 39179.0 30 45964.0 31 54295.0 32 65378.0 33 80628.0 34 104741.0 35 149449.0 36 220782.0 37 325203.0 38 115698.0 39 339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76481747703328 19.250996100648397 13.66479987620356 23.319386546114764 2 15.858468492469346 21.19753249047039 38.21402685717425 24.729972159886017 3 19.00313677989533 26.346176776495284 29.681056659193644 24.96962978441574 4 12.6932926548496 17.488284667525416 35.39612523274224 34.42229744488274 5 12.775238052498503 39.21367979621312 32.73645524683886 15.274626904449512 6 31.186532923043504 37.35982862745538 16.20448064355609 15.249157805945032 7 27.06498403570524 35.07044772630547 19.919862723002808 17.944705514986488 8 26.903363628797237 35.0891007754592 18.5094324809795 19.498103114764064 9 27.063243766528146 15.96435823573857 19.36956932376275 37.602828673970535 10-11 24.93623241906309 27.65311381003359 25.583609583225662 21.827044187677654 12-13 26.46837271604013 25.22097981443186 27.14183500764198 21.168812461886034 14-15 23.342294541573395 25.48595959309739 26.56428574801885 24.60746011731036 16-17 21.396092985457805 28.84342234960675 27.249527672570046 22.510956992365394 18-19 21.303293651320693 25.831912956346837 31.634400128682465 21.230393263650008 20-21 22.29712118962531 24.87842487638817 31.809631140705992 21.014822793280523 22-23 21.850907710536244 24.798730958623324 31.94674111520494 21.40362021563549 24-25 21.634513787310503 25.001534743644687 32.020419173906525 21.34353229513829 26-27 21.39653997948166 25.210850165339117 32.0584040791125 21.334205776066735 28-29 21.20585024407515 24.936445376391905 32.398403405092694 21.45930097444025 30-31 21.316408652648555 25.017974209223336 32.20984195682176 21.45577518130635 32-33 21.1351379430849 25.11100734814927 32.020735145499025 21.733119563266808 34-35 21.60024768401281 24.8851008267546 31.96395831636261 21.550693172869984 36-37 21.88793471870344 24.64529503849868 31.716697637189185 21.750072605608693 38-39 21.68321238372841 24.782833774276988 31.823234129865284 21.710719712129315 40-41 21.64352203700681 24.874276419807998 31.85331860372524 21.628882939459952 42-43 21.842691840442775 24.863875544704428 31.334745737698917 21.958686877153877 44-45 21.818653510371917 25.117496431721026 30.9152561782286 22.14859387967846 46-47 21.952054551936968 25.460571208647465 30.201134294949743 22.386239944465828 48-49 22.168463261425273 25.515492290968954 29.74771919582524 22.568325251780532 50-51 22.09338806899196 25.799349086806032 29.507759809666773 22.599503034535235 52-53 22.351767200364563 25.82415831017442 29.246050391596206 22.578024097864816 54-55 22.012739552822925 25.720207969569575 29.318325901632374 22.948726575975122 56-57 22.142610481950115 26.031268170159766 29.211802450333668 22.614318897556455 58-59 22.226606736877276 25.448034597854253 29.37686143061171 22.948497234656763 60-61 22.27482654445385 25.522698268218885 29.56831202991097 22.634163157416296 62-63 22.260895794448242 25.582081041547873 29.58413169517158 22.572891468832307 64-65 22.302669151795005 25.421770845784803 29.589466833719236 22.68609316870096 66-67 22.24215421340256 25.889856170909052 29.38724179413893 22.480747821549453 68-69 22.290564608790177 25.739595634102074 29.358954644824387 22.61088511228336 70-71 22.132006842595512 25.7975649521219 29.341760256514693 22.728667948767896 72-73 22.322369889035414 25.769692111349464 29.105919882128905 22.80201811748622 74-75 22.214892513011954 26.15602304342513 28.989285244144252 22.63979919941866 76-77 22.26009264019843 25.853870033369127 29.31518622781319 22.57085109861925 78-79 22.337001820470018 25.903564673909194 29.1754817398121 22.583951765808692 80-81 21.948222674258673 26.400096336497718 29.112747939567736 22.53893304967587 82-83 22.158097314221 26.148293818006664 28.875333918308332 22.818274949464005 84-85 22.053046099903046 25.953098782976724 28.840101706370813 23.153753410749413 86-87 22.176582951710717 25.709351731498238 29.08905826282317 23.025007053967872 88-89 22.202841164657926 25.64025372854163 28.973392267489288 23.18351283931116 90-91 21.913246844626094 26.041795791732923 29.15236698790032 22.892590375740664 92-93 21.90871545659982 25.925998077872155 29.060613144615328 23.104673320912703 94-95 21.81897714818863 25.859197233496626 29.075204274904152 23.246621343410588 96-97 22.092993053030266 25.729260627069095 28.889134202609718 23.288612117290917 98-99 21.928423391435906 25.835161766936842 28.682441665736956 23.553973175890295 100-101 21.770270744894518 25.917936255283458 28.922448129093837 23.38934487072819 102-103 22.192042165373003 25.61251856821193 28.92727458935955 23.26816467705552 104-105 21.937675943476137 25.639948739168904 29.021073962815237 23.40130135453972 106-107 21.86386698976685 25.964356657743043 28.873232369444008 23.298543983046095 108-109 22.06064441367553 25.80730281976364 28.415317804508234 23.7167349620526 110-111 21.686168430901184 26.006602413407887 28.726902989975372 23.580326165715558 112-113 21.74812879476349 25.988582836711426 28.61259418008611 23.65069418843898 114-115 21.451424105458948 26.244524317314227 28.37110504161253 23.932946535614295 116-117 21.427858849252708 26.124068576609737 28.585954226771747 23.862118347365804 118-119 21.435395739850424 26.291927297240896 28.35432397596645 23.918352986942224 120-121 21.349542109836964 26.142393450210644 28.363125318799238 24.144939121153154 122-123 21.336972687430624 26.202877265950974 28.163831144280337 24.29631890233806 124-125 21.236792085286236 26.244288868162872 28.144280603992776 24.374638442558112 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 774.0 1 614.0 2 1175.0 3 2105.0 4 1574.0 5 1272.0 6 3228.5 7 7468.0 8 11544.5 9 12517.0 10 8903.0 11 4482.5 12 3081.0 13 2845.0 14 2914.0 15 3079.5 16 3202.5 17 3315.0 18 3462.5 19 3585.5 20 3694.5 21 4040.0 22 4540.0 23 5189.0 24 6118.5 25 7278.0 26 8837.0 27 10499.5 28 11923.5 29 13697.5 30 16043.0 31 18748.5 32 21869.0 33 24602.0 34 27285.0 35 29554.5 36 32543.0 37 36636.0 38 39362.5 39 41225.0 40 43252.0 41 46764.0 42 50305.0 43 53163.5 44 56518.5 45 59231.0 46 59256.0 47 60156.0 48 67409.0 49 69322.5 50 66579.0 51 63822.5 52 58617.5 53 57883.0 54 57561.0 55 53746.5 56 49050.5 57 44580.0 58 40682.0 59 36693.5 60 31824.5 61 26442.0 62 21625.0 63 16900.5 64 12666.0 65 9747.5 66 7540.0 67 6119.0 68 4664.0 69 3279.5 70 2574.5 71 2227.0 72 1986.5 73 1887.0 74 1565.5 75 1211.5 76 882.5 77 560.5 78 398.0 79 260.0 80 192.0 81 155.0 82 114.5 83 76.0 84 51.5 85 38.5 86 26.0 87 19.0 88 13.0 89 5.5 90 3.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05855637290492735 2 0.07160169388475492 3 0.0 4 0.009444576274988284 5 0.03305601696245899 6 4.1320021203073733E-4 7 5.902860171867677E-5 8 5.902860171867677E-5 9 8.264004240614747E-4 10-11 0.060888002672815085 12-13 0.00950360487670696 14-15 0.007437603816553273 16-17 0.05212225531759159 18-19 8.854290257801516E-5 20-21 0.010772719813658509 22-23 0.012809206572952859 24-25 0.0 26-27 0.0 28-29 0.05108925478751475 30-31 0.0 32-33 0.0036597733065579594 34-35 0.0 36-37 5.902860171867677E-4 38-39 0.0 40-41 2.9514300859338384E-5 42-43 0.0033056016962458986 44-45 0.0 46-47 1.1805720343735354E-4 48-49 5.607717163274293E-4 50-51 7.378575214834596E-4 52-53 1.1805720343735354E-4 54-55 0.0 56-57 0.0023021154670283942 58-59 2.9514300859338384E-5 60-61 0.0 62-63 0.0 64-65 9.739719283581667E-4 66-67 0.0 68-69 5.607717163274293E-4 70-71 0.0 72-73 0.002361144068747071 74-75 0.004427145128900758 76-77 7.673718223427979E-4 78-79 0.0015347436446855958 80-81 0.0018889152549976566 82-83 2.656287077340455E-4 84-85 8.854290257801516E-5 86-87 7.083432206241212E-4 88-89 0.0 90-91 0.0 92-93 0.008145947037177394 94-95 0.006758774896788489 96-97 0.006965375002803858 98-99 0.001239600636092212 100-101 5.017431146087525E-4 102-103 1.4757150429669193E-4 104-105 7.673718223427979E-4 106-107 0.0052535455529622325 108-109 0.006168488879601723 110-111 9.739719283581667E-4 112-113 0.003866373412573328 114-115 7.673718223427979E-4 116-117 0.002774344280777808 118-119 0.002685801378199793 120-121 0.014048807209045071 122-123 0.0012100863352328736 124-125 0.00200697245843501 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1694094.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.63835748388519 #Duplication Level Percentage of deduplicated Percentage of total 1 71.37600553913562 29.006036288711627 2 11.513087043727108 9.357458940521383 3 5.3215593951450995 6.487782992149032 4 3.0777053829295298 5.00291566326272 5 1.949087148882582 3.960385016176846 6 1.3667897541599932 3.3326454380919213 7 0.9634309399797165 2.7406575664951878 8 0.7594692584439574 2.469086657813339 9 0.5579764713704787 2.0407722580035306 >10 2.8692227408984627 20.419051413161625 >50 0.15818988472153897 4.433249274734684 >100 0.08216602429299696 5.953417098353091 >500 0.00324525441339907 0.8866054339921187 >1k 0.001622627206699535 1.3089869003821437 >5k 0.0 0.0 >10k+ 4.425346927362369E-4 2.6009490581507806 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17660 1.0424451063518316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14949 0.8824185670924989 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10779 0.6362692979256169 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4898 0.2891220912180788 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3574 0.21096822254255077 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2403 0.14184572992998026 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2346 0.13848109963201571 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.902860171867677E-5 0.0 0.0 0.0 0.0 9 5.902860171867677E-5 0.0 0.0 0.0 0.0 10-11 5.902860171867677E-5 0.0 0.0 0.0 0.0 12-13 5.902860171867677E-5 0.0 0.0 0.0 5.902860171867677E-5 14-15 5.902860171867677E-5 0.0 0.0 0.0 5.902860171867677E-5 16-17 5.902860171867677E-5 0.0 0.0 5.902860171867677E-5 5.902860171867677E-5 18-19 5.902860171867677E-5 0.0 0.0 8.854290257801516E-5 5.902860171867677E-5 20-21 5.902860171867677E-5 0.0 0.0 2.656287077340455E-4 5.902860171867677E-5 22-23 5.902860171867677E-5 0.0 0.0 7.968861232021363E-4 5.902860171867677E-5 24-25 5.902860171867677E-5 0.0 0.0 0.00200697245843501 5.902860171867677E-5 26-27 5.902860171867677E-5 0.0 0.0 0.0031875444928085453 5.902860171867677E-5 28-29 5.902860171867677E-5 0.0 0.0 0.00528305985382157 5.902860171867677E-5 30-31 5.902860171867677E-5 0.0 0.0 0.010920291317955202 5.902860171867677E-5 32-33 5.902860171867677E-5 0.0 0.0 0.018210323630211783 5.902860171867677E-5 34-35 5.902860171867677E-5 0.0 0.0 0.02721218539230999 5.902860171867677E-5 36-37 5.902860171867677E-5 0.0 0.0 0.04223496452971323 5.902860171867677E-5 38-39 5.902860171867677E-5 0.0 0.0 0.06785337767561894 5.902860171867677E-5 40-41 5.902860171867677E-5 0.0 0.0 0.1040379105291678 5.902860171867677E-5 42-43 5.902860171867677E-5 0.0 0.0 0.13166329613350852 5.902860171867677E-5 44-45 5.902860171867677E-5 0.0 0.0 0.16268282633667316 5.902860171867677E-5 46-47 5.902860171867677E-5 0.0 0.0 0.19827707317303528 5.902860171867677E-5 48-49 5.902860171867677E-5 0.0 0.0 0.24071863780876385 5.902860171867677E-5 50-51 5.902860171867677E-5 0.0 0.0 0.2907158634644831 5.902860171867677E-5 52-53 5.902860171867677E-5 0.0 0.0 0.33492828615177195 5.902860171867677E-5 54-55 5.902860171867677E-5 0.0 0.0 0.3800851664665597 5.902860171867677E-5 56-57 5.902860171867677E-5 0.0 0.0 0.43356507962368085 5.902860171867677E-5 58-59 5.902860171867677E-5 0.0 0.0 0.49510239691540137 5.902860171867677E-5 60-61 5.902860171867677E-5 0.0 0.0 0.5673238911182024 5.902860171867677E-5 62-63 5.902860171867677E-5 0.0 0.0 0.6406669287536583 5.902860171867677E-5 64-65 5.902860171867677E-5 0.0 0.0 0.7120029939306791 5.902860171867677E-5 66-67 5.902860171867677E-5 0.0 0.0 0.7924589780732356 5.902860171867677E-5 68-69 5.902860171867677E-5 0.0 0.0 0.890269371121083 5.902860171867677E-5 70-71 5.902860171867677E-5 0.0 0.0 1.0114846047503856 5.902860171867677E-5 72-73 5.902860171867677E-5 0.0 0.0 1.1324932382736732 5.902860171867677E-5 74-75 5.902860171867677E-5 0.0 0.0 1.2508750990204796 5.902860171867677E-5 76-77 5.902860171867677E-5 0.0 0.0 1.3850471107270317 5.902860171867677E-5 78-79 5.902860171867677E-5 0.0 0.0 1.5439816208545687 5.902860171867677E-5 80-81 5.902860171867677E-5 0.0 0.0 1.7243139991051264 5.902860171867677E-5 82-83 5.902860171867677E-5 0.0 0.0 1.9141794965332504 5.902860171867677E-5 84-85 5.902860171867677E-5 0.0 0.0 2.1047828514828577 5.902860171867677E-5 86-87 5.902860171867677E-5 0.0 0.0 2.301230038002614 5.902860171867677E-5 88-89 5.902860171867677E-5 0.0 0.0 2.5295526694504558 5.902860171867677E-5 90-91 5.902860171867677E-5 0.0 0.0 2.7900458888349764 5.902860171867677E-5 92-93 5.902860171867677E-5 0.0 0.0 3.0765116929757146 5.902860171867677E-5 94-95 5.902860171867677E-5 0.0 0.0 3.35863889489013 5.902860171867677E-5 96-97 5.902860171867677E-5 0.0 0.0 3.6415629829277476 5.902860171867677E-5 98-99 5.902860171867677E-5 0.0 0.0 3.954975343753062 5.902860171867677E-5 100-101 8.854290257801516E-5 0.0 0.0 4.331164622506189 5.902860171867677E-5 102-103 1.1805720343735354E-4 0.0 0.0 4.743331834006849 5.902860171867677E-5 104-105 1.1805720343735354E-4 0.0 0.0 5.148356584699551 5.902860171867677E-5 106-107 1.1805720343735354E-4 0.0 0.0 5.5397457283952365 5.902860171867677E-5 108-109 1.1805720343735354E-4 0.0 0.0 5.955041455786986 5.902860171867677E-5 110-111 1.1805720343735354E-4 0.0 0.0 6.441850334160915 5.902860171867677E-5 112-113 1.1805720343735354E-4 0.0 0.0 6.968533032995808 5.902860171867677E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9950 0.0 45.459312 1 GTATCAA 14945 0.0 40.314327 1 TATCAAC 18540 0.0 32.22097 2 ATCAACG 18810 0.0 31.854996 3 TCAACGC 19140 0.0 31.15125 4 GTACATG 11695 0.0 30.778036 1 CAACGCA 19365 0.0 30.51176 5 AACGCAG 19580 0.0 30.318968 6 TACATGG 11835 0.0 29.490135 2 GAGTACT 13360 0.0 28.501389 12-13 GTACACG 275 1.8189894E-12 28.171814 1 ACATGGG 11980 0.0 27.516289 3 ATGGGAG 2760 0.0 27.407393 5 ACGCAGA 22120 0.0 26.702902 7 CGCAGAG 22145 0.0 26.592094 8 GTACTTT 14465 0.0 26.32413 14-15 AGTACTT 13795 0.0 25.466087 12-13 GCAGAGT 22775 0.0 25.30748 9 CATGGGG 3745 0.0 23.992464 4 ACTTTTT 16405 0.0 23.956606 16-17 >>END_MODULE