FastQCFastQC Report
Thu 26 May 2016
SRR1364597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497327
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49550.039.674641
ATCAACG59900.033.613613
GTACATG89050.033.314871
TCAACGC61550.032.9060754
CAACGCA62200.032.6579745
TACATGG87450.032.151962
AACGCAG62950.031.0125276
ACATGGG91350.030.6488743
TATCAAC67550.028.572282
GGTATCA35600.027.610651
ACGCAGA73550.025.6502767
CGCAGAG72950.024.4694528
CATGGGG43400.024.2945464
CATGGGA54100.022.5726134
CGCATAG2454.348767E-721.8577148
GTACCTG22450.019.9015831
GCAGAGT85750.019.704669
TAGACTG13650.019.6383555
AGAGTAC81550.018.38268910-11
GTATAGG8450.018.3298661
Produced by FastQC (version 0.11.5)