##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364597_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2497327 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.195031327495357 31.0 26.0 31.0 7.0 34.0 2 25.766450288648624 31.0 25.0 31.0 7.0 34.0 3 26.39435644591197 31.0 26.0 31.0 7.0 34.0 4 29.807822523842493 35.0 30.0 37.0 7.0 37.0 5 29.94393005000947 35.0 30.0 37.0 7.0 37.0 6 30.190283050637742 35.0 30.0 37.0 7.0 37.0 7 29.89368032300135 35.0 30.0 37.0 2.0 37.0 8 29.70046253454193 35.0 30.0 37.0 2.0 37.0 9 30.69828460590063 37.0 30.0 39.0 2.0 39.0 10-11 30.632643422347172 37.0 29.0 39.0 2.0 39.0 12-13 30.638195158263215 37.0 30.0 39.0 2.0 39.0 14-15 31.25389946931259 37.0 29.0 40.0 2.0 41.0 16-17 31.622830930831245 38.0 30.0 40.0 2.0 41.0 18-19 31.427565753303433 38.0 29.5 40.0 2.0 41.0 20-21 31.352360343679464 38.0 29.0 40.0 2.0 41.0 22-23 31.386837806983227 38.0 29.0 40.0 2.0 41.0 24-25 31.476755747244955 38.0 30.0 40.0 2.0 41.0 26-27 31.28575452874213 37.5 29.5 40.0 2.0 41.0 28-29 31.177069522733706 37.0 29.0 40.0 2.0 41.0 30-31 31.04282859233092 37.0 29.0 40.0 2.0 41.0 32-33 30.969345624341546 37.0 29.0 40.0 2.0 41.0 34-35 31.022680850365212 37.0 29.0 40.0 2.0 41.0 36-37 30.951464906277792 37.0 29.0 40.0 2.0 41.0 38-39 30.660947485051018 37.0 28.0 40.0 2.0 41.0 40-41 30.53061092920551 37.0 28.0 40.0 2.0 41.0 42-43 30.01978014893524 36.5 26.5 40.0 2.0 41.0 44-45 29.85632658438402 36.0 27.0 40.0 2.0 41.0 46-47 29.814492655547312 36.0 27.0 40.0 2.0 41.0 48-49 29.75593985088857 36.0 27.0 40.0 2.0 41.0 50-51 29.52829004771902 36.0 26.0 40.0 2.0 41.0 52-53 29.427724723274125 35.5 26.5 40.0 2.0 41.0 54-55 29.25948323947965 35.0 26.0 40.0 2.0 41.0 56-57 29.035148180434522 35.0 26.0 39.0 2.0 41.0 58-59 28.834270401913727 35.0 25.5 39.0 2.0 40.0 60-61 28.54973617792143 35.0 25.0 39.0 2.0 40.0 62-63 28.371429332242034 34.0 24.5 39.0 2.0 40.0 64-65 28.148769464311243 34.0 24.5 38.5 2.0 40.0 66-67 27.883966136593244 34.0 24.0 38.0 2.0 40.0 68-69 27.643759107237457 34.0 23.0 38.0 2.0 40.0 70-71 27.213936340735515 33.5 22.0 37.0 2.0 40.0 72-73 26.980718183882207 33.0 22.0 36.5 2.0 39.0 74-75 26.955169266980256 33.0 22.5 36.0 2.0 39.0 76-77 26.709994325933287 33.0 22.0 36.0 2.0 39.0 78-79 26.423124604827482 33.0 22.0 35.5 2.0 38.5 80-81 26.022739713301462 33.0 20.0 35.0 2.0 37.0 82-83 26.13732162428068 33.0 22.0 35.0 2.0 37.0 84-85 25.979562748490686 33.0 21.0 35.0 2.0 36.5 86-87 25.84872585768704 33.0 21.0 35.0 2.0 36.0 88-89 25.68823946563666 33.0 20.0 35.0 2.0 36.0 90-91 25.55960973472837 33.0 20.0 35.0 2.0 35.5 92-93 25.43274789404832 33.0 20.0 35.0 2.0 35.0 94-95 25.326776989957665 33.0 19.0 35.0 2.0 35.0 96-97 25.21340617388111 33.0 18.5 35.0 2.0 35.0 98-99 25.12873123944121 33.0 18.0 35.0 2.0 35.0 100-101 25.022648215471982 33.0 17.5 35.0 2.0 35.0 102-103 23.888475958494823 30.5 13.0 34.0 2.0 35.0 104-105 24.729641732940863 32.0 15.0 34.5 2.0 35.0 106-107 24.89668793874411 33.0 15.0 35.0 2.0 35.0 108-109 24.8538187029572 33.0 10.5 35.0 2.0 35.0 110-111 24.788519084605262 33.0 8.5 35.0 2.0 35.0 112-113 24.699250638782985 33.0 6.0 35.0 2.0 35.0 114-115 24.599500185598444 33.0 2.0 35.0 2.0 35.0 116-117 24.49680718624353 33.0 2.0 35.0 2.0 35.0 118-119 24.370627675110228 33.0 2.0 35.0 2.0 35.0 120-121 24.1944629197538 33.0 2.0 35.0 2.0 35.0 122-123 23.98876879159197 33.0 2.0 35.0 2.0 35.0 124-125 23.053926858597215 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1460206657080434 1101 2 1.3564722560572982 1101 3 0.940108084120137 1101 4 0.9824356550596889 1101 5 0.7875792657938696 1101 6 0.8340047476107273 1101 7 0.8046822021667062 1101 8 1.0323846325295172 1101 9 1.3410395493507181 1101 10-11 1.2374653910800113 1101 12-13 1.3211700555721713 1101 14-15 1.63606165726679 1101 16-17 1.1956656267466883 1101 18-19 1.1864438387946628 1101 20-21 1.4070594111720602 1101 22-23 1.2260118700748528 1101 24-25 1.0990064635445265 1101 26-27 1.3541272845054593 1101 28-29 1.3501225070346905 1101 30-31 1.5439228992527525 1101 32-33 1.5174376065350401 1101 34-35 1.2715907648707194 1101 36-37 1.1208182485217755 1101 38-39 1.2051536680614277 1101 40-41 1.3354234476273845 1101 42-43 1.4096266364964443 1101 44-45 1.2239663017236246 1101 46-47 0.9918780684948025 1101 48-49 0.7744348923196505 1101 50-51 0.8786534834927942 1101 52-53 0.7963247348222744 1101 54-55 0.7908251127756039 1101 56-57 0.8104724216733352 1101 58-59 0.6526799031710944 1101 60-61 0.739747316114677 1101 62-63 0.6060136229648307 1101 64-65 0.6569390149557641 1101 66-67 0.6617781541914738 1101 68-69 0.5504629102632386 1101 70-71 0.4531653427908253 1101 72-73 0.427952690417964 1101 74-75 0.42474435542464306 1101 76-77 0.5109370813384935 1101 78-79 0.6580865739708415 1101 80-81 0.688519189633773 1101 82-83 0.683780197717514 1101 84-85 0.7470579229394083 1101 86-87 0.5904457316039569 1101 88-89 0.6109713788577444 1101 90-91 0.6607420969780975 1101 92-93 0.5945220016579995 1101 94-95 0.5736795021417684 1101 96-97 0.5184108182220584 1101 98-99 0.6197281920089921 1101 100-101 0.6227178004396663 1101 102-103 0.4984535281308382 1101 104-105 0.6650948682715416 1101 106-107 0.6281210610134416 1101 108-109 0.5803861349132902 1101 110-111 0.6939227281110689 1101 112-113 0.6116733658227567 1101 114-115 0.6842454295451645 1101 116-117 0.5895609081624187 1101 118-119 0.5409528401966384 1101 120-121 0.6334352379704065 1101 122-123 0.5791492631620905 1101 124-125 0.49105984742303477 1102 1 0.9252137416449919 1102 2 1.372968112935169 1102 3 1.132489322974532 1102 4 1.1684493055191219 1102 5 0.9081229923836176 1102 6 0.7437616748966036 1102 7 0.6936726697629787 1102 8 0.7823656536640939 1102 9 1.361198685346821 1102 10-11 1.3422409033303317 1102 12-13 1.5011426973586204 1102 14-15 1.6797789366285265 1102 16-17 1.4548444148208048 1102 18-19 1.241984056084764 1102 20-21 1.5310685711603078 1102 22-23 1.5227253035306596 1102 24-25 1.2030101089190453 1102 26-27 1.4730150155641937 1102 28-29 1.1205795754087404 1102 30-31 1.1609909868276809 1102 32-33 1.504138972297163 1102 34-35 1.3227307501173406 1102 36-37 1.0952794994080541 1102 38-39 1.3447502060299001 1102 40-41 1.414337749097708 1102 42-43 1.3954465592918517 1102 44-45 1.1215013768154733 1102 46-47 0.9390426976191257 1102 48-49 0.8002876879423368 1102 50-51 0.9617094747267068 1102 52-53 0.8721177291142901 1102 54-55 0.8643325861514768 1102 56-57 1.0423268358709592 1102 58-59 1.0234710711949297 1102 60-61 1.0054329902941106 1102 62-63 0.6625432478994817 1102 64-65 0.610111529261534 1102 66-67 0.7376327402731633 1102 68-69 0.5128630921738697 1102 70-71 0.7058682197003492 1102 72-73 0.4146891491765281 1102 74-75 0.6425547673018315 1102 76-77 0.2921412008265669 1102 78-79 0.3540791686324063 1102 80-81 0.5744116260187653 1102 82-83 0.6266095884601235 1102 84-85 0.613002497490541 1102 86-87 0.6839023201349939 1102 88-89 0.554993036249833 1102 90-91 0.6530438394527991 1102 92-93 0.6545696994858119 1102 94-95 0.7055636379588286 1102 96-97 0.448391875165818 1102 98-99 0.6027532083021683 1102 100-101 0.7196892390376668 1102 102-103 0.5277466907260013 1102 104-105 0.6593030914916476 1102 106-107 0.5925949525402707 1102 108-109 0.661362328970398 1102 110-111 0.6157016923663505 1102 112-113 0.4609140321599767 1102 114-115 0.7095528513558165 1102 116-117 0.7901958049245508 1102 118-119 0.8418879969334192 1102 120-121 0.8001282243844017 1102 122-123 0.705574289481838 1102 124-125 0.5167713758304551 1103 1 0.5917066734577752 1103 2 0.8525550100603745 1103 3 0.45409926595386096 1103 4 0.22239768993347298 1103 5 0.2644650570070439 1103 6 0.3270638250510345 1103 7 0.36369375553157113 1103 8 0.5326136341820344 1103 9 0.9587738219588431 1103 10-11 0.9366932673122754 1103 12-13 0.9445036500789818 1103 14-15 1.2533972248204996 1103 16-17 0.8605591035829789 1103 18-19 0.7130653699360359 1103 20-21 1.0662335450258418 1103 22-23 0.8706798354859977 1103 24-25 0.66636393405566 1103 26-27 0.9267866952980981 1103 28-29 0.9194428742920806 1103 30-31 0.9157686953095059 1103 32-33 0.9709073555235861 1103 34-35 0.7922618145031777 1103 36-37 0.6088508643403401 1103 38-39 0.6998716719362932 1103 40-41 0.6710502995847598 1103 42-43 0.9511827792966017 1103 44-45 0.6958843953471927 1103 46-47 0.5388259002800915 1103 48-49 0.34221866917303245 1103 50-51 0.39992446047153507 1103 52-53 0.2574341641344873 1103 54-55 0.415750678334895 1103 56-57 0.3373820746450171 1103 58-59 0.32907439146102746 1103 60-61 0.35650361276975673 1103 62-63 0.07582653965524244 1103 64-65 0.009253249385078988 1103 66-67 -0.0069547508173570805 1103 68-69 0.012286945820818573 1103 70-71 0.14744653402679475 1103 72-73 0.05159221403754444 1103 74-75 0.16142059053775526 1103 76-77 0.12171102144904111 1103 78-79 0.14130000493556594 1103 80-81 0.21351384744903612 1103 82-83 0.23639590943897915 1103 84-85 0.3275509353617707 1103 86-87 0.20691996090477005 1103 88-89 0.23052537766109538 1103 90-91 0.31322069262456864 1103 92-93 0.2768099881529551 1103 94-95 0.25775462750503664 1103 96-97 0.3383768775416769 1103 98-99 0.2731997217257849 1103 100-101 0.1804949529839739 1103 102-103 0.060846150217567896 1103 104-105 0.10765409529302516 1103 106-107 0.22246546810216117 1103 108-109 0.348483676083724 1103 110-111 0.28403665910429154 1103 112-113 0.3282622740226522 1103 114-115 0.3149289798899133 1103 116-117 0.2781368241690316 1103 118-119 0.3462246861707676 1103 120-121 0.40511827546541923 1103 122-123 0.29783195163436815 1103 124-125 0.16721091016165346 1104 1 0.9457231014887384 1104 2 1.2606653499707434 1104 3 0.7086518967669662 1104 4 0.8648984902361327 1104 5 0.40792767559734955 1104 6 0.6305273166987853 1104 7 0.44917226376741226 1104 8 0.7136031720436229 1104 9 0.9761349330699538 1104 10-11 1.0558801598097993 1104 12-13 0.910147020662766 1104 14-15 0.9639977792119758 1104 16-17 0.7890111363517391 1104 18-19 0.7877128729917366 1104 20-21 1.1244491974907724 1104 22-23 0.9432467335128827 1104 24-25 0.9129586462377262 1104 26-27 1.1891928416225213 1104 28-29 1.1719687386189577 1104 30-31 1.3045137116065781 1104 32-33 1.3055857429864872 1104 34-35 1.176921831382284 1104 36-37 0.9573035519021822 1104 38-39 1.0703328645327659 1104 40-41 1.1324457000271089 1104 42-43 1.3772831954539875 1104 44-45 1.204362328820217 1104 46-47 1.0036365923236268 1104 48-49 0.8573553026635139 1104 50-51 0.868434187786594 1104 52-53 0.792597462254502 1104 54-55 0.8080259387970337 1104 56-57 0.7256634610602397 1104 58-59 0.6834311300094242 1104 60-61 0.7018585833768256 1104 62-63 0.5765484490318791 1104 64-65 0.4897047655948441 1104 66-67 0.5139225991622709 1104 68-69 0.33894721006620543 1104 70-71 0.32632490984892115 1104 72-73 0.34330689886380483 1104 74-75 0.40877922798609845 1104 76-77 0.4792733359243364 1104 78-79 0.5150959132063306 1104 80-81 0.5605523638334162 1104 82-83 0.5796353527193467 1104 84-85 0.6153501394243968 1104 86-87 0.5350786099938816 1104 88-89 0.501370897421296 1104 90-91 0.5673853585345867 1104 92-93 0.5877801368637421 1104 94-95 0.43678469705861644 1104 96-97 0.461388671092692 1104 98-99 0.4769933382207512 1104 100-101 0.5132618902984483 1104 102-103 0.4541248810830574 1104 104-105 0.40705914302016666 1104 106-107 0.46832135541954756 1104 108-109 0.47853758738109065 1104 110-111 0.44167827629770073 1104 112-113 0.507441672777091 1104 114-115 0.4982584935969143 1104 116-117 0.4386793566176621 1104 118-119 0.44740240428961897 1104 120-121 0.5784716527426461 1104 122-123 0.4422960398713762 1104 124-125 0.15263230588949384 1105 1 1.0061741544542535 1105 2 1.298468272163209 1105 3 0.7567590580923458 1105 4 0.9590314601422314 1105 5 0.7004110006803863 1105 6 0.6176698355450156 1105 7 0.5834833433962245 1105 8 0.8710832902127059 1105 9 0.9050444511491449 1105 10-11 0.8559008373281962 1105 12-13 0.9985884259607651 1105 14-15 1.291312401289769 1105 16-17 0.877234202244324 1105 18-19 0.9865079501544365 1105 20-21 0.9888657428194172 1105 22-23 1.1033344282837128 1105 24-25 0.7699256926941302 1105 26-27 1.1832366807231267 1105 28-29 1.1404750485414823 1105 30-31 1.0629748554772327 1105 32-33 1.0866076128278905 1105 34-35 1.0422717647240454 1105 36-37 0.6197830038340264 1105 38-39 0.9006765186748638 1105 40-41 0.9352644703810036 1105 42-43 1.073783654916042 1105 44-45 0.8007307835571638 1105 46-47 0.3880637974480585 1105 48-49 0.5166454803935174 1105 50-51 0.6717351343957922 1105 52-53 0.47765224214421664 1105 54-55 0.5608855809068167 1105 56-57 0.7464412602404558 1105 58-59 0.6807971110385758 1105 60-61 0.7225538796038506 1105 62-63 0.5697942532906879 1105 64-65 0.2806434213697422 1105 66-67 0.17920224965352816 1105 68-69 0.11692178672939946 1105 70-71 0.4284157556112014 1105 72-73 0.3511785090798085 1105 74-75 0.45842529376891505 1105 76-77 0.5097932495137094 1105 78-79 0.4685201654844846 1105 80-81 0.6136375945620713 1105 82-83 0.6485922875585857 1105 84-85 0.5070630802369855 1105 86-87 0.633072925229726 1105 88-89 0.5975673731989097 1105 90-91 0.442358933425524 1105 92-93 0.5491164913930859 1105 94-95 0.3525040641263324 1105 96-97 0.42930430819845 1105 98-99 0.530989553440925 1105 100-101 0.4188869692630952 1105 102-103 0.3168868452194751 1105 104-105 0.4944256970823844 1105 106-107 0.5178698675012257 1105 108-109 0.410785092098255 1105 110-111 0.5094748191823228 1105 112-113 0.45772235541826234 1105 114-115 0.5970714866728812 1105 116-117 0.5098941452892838 1105 118-119 0.43769853811147996 1105 120-121 0.28196446186809254 1105 122-123 0.31517700847675556 1105 124-125 0.23865910174404448 1106 1 0.15850547882568478 1106 2 -2.2780376022622413 1106 3 -0.4399777277442851 1106 4 0.10011366445403169 1106 5 0.19398884764040503 1106 6 0.3451320686925108 1106 7 0.4534648599428692 1106 8 0.3846155496737147 1106 9 0.8810300784760479 1106 10-11 1.0266375027023997 1106 12-13 0.9828426694379147 1106 14-15 1.072806737673556 1106 16-17 0.7467277518579039 1106 18-19 0.7960761488270869 1106 20-21 1.0753549407278804 1106 22-23 0.729857619182777 1106 24-25 0.8784924533465883 1106 26-27 0.8532397487505179 1106 28-29 0.9571773520089124 1106 30-31 1.1516639043134553 1106 32-33 1.2206215902336552 1106 34-35 0.8973902884570251 1106 36-37 0.7203564237999203 1106 38-39 1.140306397873637 1106 40-41 1.1198067884545075 1106 42-43 1.3020580683204983 1106 44-45 1.0773459211666605 1106 46-47 0.7794960956829087 1106 48-49 0.6511982448179516 1106 50-51 0.7014785306080036 1106 52-53 0.7084022524964588 1106 54-55 0.696351224646726 1106 56-57 0.6433875377632994 1106 58-59 0.644210884411109 1106 60-61 0.6772971817047875 1106 62-63 0.6756209643276812 1106 64-65 0.6039085742252652 1106 66-67 0.5488628467707102 1106 68-69 0.48305132900982883 1106 70-71 0.574213405088134 1106 72-73 0.3659323328206945 1106 74-75 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70-71 -4.367345223332951 2216 72-73 -2.45018446333091 2216 74-75 -1.915420365548659 2216 76-77 -1.814101917278716 2216 78-79 -1.9271367638515038 2216 80-81 -1.7811863892598936 2216 82-83 -1.8717060998661275 2216 84-85 -1.8419998278204694 2216 86-87 -2.0310395128939973 2216 88-89 -1.9341963192970582 2216 90-91 -1.7663738753136116 2216 92-93 -1.7714587377000122 2216 94-95 -2.043891281783747 2216 96-97 -1.9777844586394195 2216 98-99 -2.0794875708138036 2216 100-101 -2.066934238396559 2216 102-103 -1.770626104503009 2216 104-105 -1.8203011952875912 2216 106-107 -1.8850411978389587 2216 108-109 -2.038128325331087 2216 110-111 -1.8850073565130572 2216 112-113 -2.1355263612279316 2216 114-115 -2.077178171712635 2216 116-117 -1.8232749269328892 2216 118-119 -1.9010247628687935 2216 120-121 -2.073254316633399 2216 122-123 -1.904497726767982 2216 124-125 -1.930670953036909 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 246551.0 3 31514.0 4 18602.0 5 14982.0 6 14507.0 7 15964.0 8 17387.0 9 18533.0 10 19477.0 11 20655.0 12 21716.0 13 22001.0 14 21385.0 15 18428.0 16 15153.0 17 13198.0 18 12107.0 19 11510.0 20 11275.0 21 12421.0 22 15135.0 23 21206.0 24 30226.0 25 39187.0 26 45222.0 27 49880.0 28 57210.0 29 65519.0 30 76033.0 31 87028.0 32 105546.0 33 133122.0 34 173517.0 35 234944.0 36 335303.0 37 362305.0 38 88488.0 39 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36394607909026 18.87942023426394 13.912194957543523 23.844438729102276 2 13.425400393368925 20.76433990286196 41.780114799502265 24.030144904266848 3 19.540855831108455 25.310157653098926 27.903064388134414 27.245922127658208 4 11.798858642578125 16.82189138312089 37.40041632401316 33.978833650287825 5 13.445141532985133 38.68686085619111 33.63593019010179 14.232067420721966 6 31.79665947211986 37.077748462697386 17.71688588763825 13.408706177544506 7 28.477895681388965 32.47092740941929 20.428225273750673 18.62295163544107 8 25.27647257782819 37.21124499023223 19.511743990986933 18.00053844095265 9 25.43115674987753 17.660645885549926 19.96410721615071 36.944090148421836 10-11 24.20292197116364 27.949980672035725 27.487312514736573 20.359784842064066 12-13 24.817081730634246 24.98112859429085 27.89061403262487 22.311175642450035 14-15 21.88005211881099 26.536205163260412 25.589265097486084 25.994477620442513 16-17 22.254179706001846 29.72559343262679 25.79456224129377 22.225664620077588 18-19 22.11799096678796 28.479449422557195 27.662878023556637 21.739681587098207 20-21 22.83005168668682 27.624317813956008 27.7544471209292 21.791183378427977 22-23 22.60643852014302 27.441738139243483 27.446492612811923 22.505330727801574 24-25 22.43327931368366 27.52227934437436 27.5482761754088 22.49616516653317 26-27 22.35932414014834 27.71897601103327 27.536807493213082 22.38489235560531 28-29 22.188540857779117 27.693415040334745 27.784144146167716 22.33389995571842 30-31 22.41688273642871 27.457826544388325 27.695294414642603 22.42999630454036 32-33 22.242713577919083 27.482277897017234 27.694562012316208 22.580446512747475 34-35 22.650128768307678 27.562914406570993 27.439098726112093 22.347858099009237 36-37 22.582250541479475 27.49959797580316 27.379832862199738 22.538318620517632 38-39 22.3360948378639 27.607022340748156 27.63363852691945 22.423244294468486 40-41 22.526395274820004 27.440593704855598 27.649312425885697 22.3836985944387 42-43 22.39818414098402 27.29576002460331 27.66127652945612 22.644779304956554 44-45 22.541923720067594 27.518621957123475 27.458537542130284 22.48091678067865 46-47 22.451974276664018 27.768044824743683 27.011608225836948 22.768372672755348 48-49 22.660187820239283 27.554575030239253 27.15017517910941 22.635061970412057 50-51 22.52377740005952 27.65724527881142 27.14301413288392 22.675963188245138 52-53 22.727077623665785 27.681130177210967 26.947571612870096 22.64422058625315 54-55 22.664005259174374 27.518894642837804 27.077327752212348 22.739772345775474 56-57 22.51039780490451 27.866578399719717 27.097304281715683 22.525719513660086 58-59 22.708505930388014 27.55045982662367 27.054623408932514 22.6864108340558 60-61 22.790194313430092 27.546254288448857 27.241458344320357 22.422093053800694 62-63 22.607831060061066 27.649470959939244 27.1573831023169 22.585314877682794 64-65 22.507882833437286 27.484869390667548 27.11365895996521 22.89358881592995 66-67 22.458665997362612 27.78616858042454 26.954717330631528 22.800448091581323 68-69 22.50965785047422 27.614736466479687 27.116036542459458 22.759569140586628 70-71 22.446599201208333 27.475013201594056 27.28004660754117 22.798340989656445 72-73 22.644676667113632 27.50171020190304 27.028746424510498 22.824866706472832 74-75 22.541270133972596 27.65169129378563 26.96898025287227 22.8380583193695 76-77 22.705911764287237 27.528577680630452 27.001279652996118 22.764230902086197 78-79 22.680386328108632 27.60309644709742 27.03446683003925 22.6820503947547 80-81 22.4304606614621 27.779382970894982 27.133580695709185 22.656575671933727 82-83 22.70676670622773 27.83027522107977 26.91636053462175 22.546597538070756 84-85 22.687648787983164 27.55490294121539 26.992911907890488 22.76453636291096 86-87 22.509913580291478 27.502760401187665 27.23863222071017 22.74869379781069 88-89 22.725766947685972 27.462628683540657 27.088725017776767 22.722879350996603 90-91 22.59483589688476 27.76230753313582 27.18220009335615 22.460656476623267 92-93 22.67481822016974 27.581409934570488 27.156896084334935 22.586875760924833 94-95 22.488287089346215 27.636452751877215 27.132279181401813 22.74298097737476 96-97 22.635929575236432 27.612328925619504 27.038530048191888 22.71321145095218 98-99 22.551352053691147 27.616929048837314 26.90119548258729 22.930523414884252 100-101 22.456402589932846 27.58953690225283 27.17384614490176 22.780214362912567 102-103 22.491881736474106 27.427156233254074 27.232963679594924 22.847998350676896 104-105 22.42979300501074 27.41022215049862 27.288677371080443 22.871307473410198 106-107 22.435000142343505 27.601026797868617 27.2142133396312 22.749759720156682 108-109 22.435902319935614 27.562353307270666 26.987893250801108 23.013851121992612 110-111 22.281741620951344 27.67919981766658 27.173032133058495 22.86602642832358 112-113 22.435583285678863 27.64929277963151 27.161663799381003 22.75346013530862 114-115 22.247093991637044 27.645893706057105 27.13915367479725 22.967858627508594 116-117 22.144826640667844 27.603256320375614 27.27148270255188 22.98043433640466 118-119 22.23593706191258 27.64112038950412 27.097824350781373 23.025118197801923 120-121 22.223480283332282 27.69916565832512 27.156905693378935 22.92044836496366 122-123 22.243846254533413 27.6482418285312 27.111859366327433 22.996052550607953 124-125 22.290197367563888 27.6463319178619 27.058628980449654 23.004841734124554 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3800.0 1 2755.0 2 1355.0 3 894.5 4 702.5 5 596.0 6 604.0 7 716.0 8 886.0 9 973.5 10 996.5 11 1010.5 12 1078.5 13 1206.0 14 1338.5 15 1451.0 16 1566.5 17 1725.5 18 1948.0 19 2264.0 20 2718.0 21 3408.5 22 4319.0 23 5642.0 24 7398.0 25 9530.0 26 12720.5 27 16612.5 28 20240.5 29 24075.5 30 28849.0 31 33662.5 32 38046.5 33 42944.5 34 48772.5 35 54163.5 36 59867.0 37 66470.0 38 71531.5 39 75078.0 40 78060.0 41 81489.0 42 85319.5 43 89333.0 44 93441.0 45 96200.0 46 96288.5 47 96255.0 48 97845.5 49 97483.0 50 94625.0 51 90755.5 52 87138.5 53 84304.5 54 80724.0 55 74306.5 56 66765.0 57 60180.5 58 54143.5 59 47940.5 60 40896.5 61 33213.0 62 26189.0 63 20794.0 64 16372.0 65 12637.0 66 9611.0 67 7547.5 68 5959.0 69 4463.5 70 3377.0 71 2672.5 72 2131.5 73 1745.5 74 1385.0 75 1065.5 76 751.0 77 529.0 78 402.0 79 284.0 80 192.0 81 127.0 82 92.0 83 70.0 84 46.5 85 37.5 86 28.0 87 20.0 88 17.0 89 10.5 90 8.0 91 6.0 92 7.0 93 7.0 94 5.0 95 2.5 96 2.0 97 1.5 98 1.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.200614497020214 2 0.24133803863090417 3 0.29243266900970516 4 0.27865794107059266 5 0.31097249178822 6 0.22524082749275526 7 0.21867380603341094 8 0.19805175693851867 9 0.19636995875990607 10-11 0.14139117544478558 12-13 0.1656571205933384 14-15 0.18263527363457008 16-17 0.1565674018660752 18-19 0.18123377515239295 20-21 0.177069322519638 22-23 0.19771139302141852 24-25 0.18882188836303776 26-27 0.23835484900455567 28-29 0.16781943253726886 30-31 0.14921954553808933 32-33 0.1872802400326429 34-35 0.14819845378678884 36-37 0.14799823971790638 38-39 0.17951193416000388 40-41 0.1993131055724781 42-43 0.2644827849937153 44-45 0.16689844782040958 46-47 0.15190241406111413 48-49 0.15532607463900402 50-51 0.15989095540952386 52-53 0.17935176290489793 54-55 0.16657810531019768 56-57 0.1654769279313442 58-59 0.14259245985808025 60-61 0.15438506851525652 62-63 0.14275263111318623 64-65 0.13848807144599004 66-67 0.1278967472021085 68-69 0.12981880226338 70-71 0.1325417136001813 72-73 0.10965724552691739 74-75 0.11159932199507713 76-77 0.13180092154531625 78-79 0.13760712954290727 80-81 0.14753774735947675 82-83 0.14869898895899497 84-85 0.13718667999825412 86-87 0.16137253951925398 88-89 0.15632714498341627 90-91 0.14619631309796435 92-93 0.14619631309796435 94-95 0.16569716340711488 96-97 0.15446515414280948 98-99 0.14941975960697176 100-101 0.1315606646626573 102-103 0.16749909002705693 104-105 0.12751634047123184 106-107 0.1345438542890058 108-109 0.14391387271270442 110-111 0.11994824866747525 112-113 0.11242019967749518 114-115 0.11027790914045296 116-117 0.11432223333187844 118-119 0.09860542892460619 120-121 0.09233872856858553 122-123 0.11738550858577991 124-125 0.11490285413163755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2497327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.11861870650799 #Duplication Level Percentage of deduplicated Percentage of total 1 82.58265800450157 54.602512763695884 2 10.04113287665042 13.27811672105262 3 3.2315415902157816 6.4099519871309925 4 1.3943357442829425 3.687662137003956 5 0.7246022922921868 2.395485133896438 6 0.44476155429653613 1.7644211774307916 7 0.3075331145139631 1.423356531672151 8 0.20478662576478862 1.0832167060107525 9 0.1629070643672472 0.9694071066145116 >10 0.8377147485089318 9.77952517489062 >50 0.050124984343530125 2.270892619386565 >100 0.017279286956485652 1.885248274274577 >500 4.3547931394798237E-4 0.20755849277156352 >1k 1.866339916909683E-4 0.24264517416856374 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.0042813776489825E-5 0.0 0.0 0.0 0.0 3 4.0042813776489825E-5 0.0 0.0 0.0 0.0 4 4.0042813776489825E-5 0.0 0.0 0.0 0.0 5 4.0042813776489825E-5 0.0 0.0 0.0 0.0 6 4.0042813776489825E-5 0.0 0.0 0.0 0.0 7 8.008562755297965E-5 0.0 0.0 0.0 4.0042813776489825E-5 8 8.008562755297965E-5 0.0 0.0 0.0 4.0042813776489825E-5 9 8.008562755297965E-5 0.0 0.0 0.0 4.0042813776489825E-5 10-11 8.008562755297965E-5 0.0 0.0 0.0 4.0042813776489825E-5 12-13 1.2012844132946947E-4 0.0 0.0 0.0 4.0042813776489825E-5 14-15 1.2012844132946947E-4 0.0 0.0 0.0 4.0042813776489825E-5 16-17 1.601712551059593E-4 0.0 0.0 0.0 4.0042813776489825E-5 18-19 1.601712551059593E-4 0.0 0.0 0.0 4.0042813776489825E-5 20-21 1.601712551059593E-4 0.0 0.0 2.0021406888244913E-5 8.008562755297965E-5 22-23 1.601712551059593E-4 0.0 0.0 1.2012844132946947E-4 8.008562755297965E-5 24-25 2.002140688824491E-4 0.0 0.0 2.2023547577069402E-4 1.0010703444122455E-4 26-27 2.002140688824491E-4 0.0 0.0 3.003211033236737E-4 1.2012844132946947E-4 28-29 3.603853239884084E-4 0.0 0.0 6.006422066473473E-4 1.2012844132946947E-4 30-31 3.603853239884084E-4 0.0 0.0 0.001821948026830287 1.2012844132946947E-4 32-33 3.603853239884084E-4 0.0 0.0 0.004304602480972656 1.2012844132946947E-4 34-35 4.6049235842963293E-4 0.0 0.0 0.007287792107321148 1.2012844132946947E-4 36-37 6.80727834200327E-4 0.0 0.0 0.013234149953129886 1.2012844132946947E-4 38-39 8.008562755297964E-4 0.0 0.0 0.02372536716257022 1.2012844132946947E-4 40-41 8.008562755297964E-4 0.0 0.0 0.03555801863352297 1.2012844132946947E-4 42-43 9.00963309971021E-4 0.0 0.0 0.0476109055802464 1.2012844132946947E-4 44-45 0.0010411131581887353 0.0 0.0 0.06108531241603522 1.2012844132946947E-4 46-47 0.0013013914477359194 0.0 0.0 0.07824365811926112 1.2012844132946947E-4 48-49 0.001361455668400654 0.0 0.0 0.09754429435952922 1.2012844132946947E-4 50-51 0.001361455668400654 0.0 0.0 0.11980809881925755 1.2012844132946947E-4 52-53 0.001361455668400654 0.0 0.0 0.14153132529300327 1.2012844132946947E-4 54-55 0.001361455668400654 0.0 0.0 0.16535679949001472 1.4014984821771439E-4 56-57 0.0014815841097301234 0.0 0.0 0.19386728289887548 1.601712551059593E-4 58-59 0.0014815841097301234 0.0 0.0 0.2260617051751733 1.601712551059593E-4 60-61 0.0014815841097301234 0.0 0.0 0.2629211152564322 1.601712551059593E-4 62-63 0.0015216269235066133 0.0 0.0 0.30098180975098576 1.601712551059593E-4 64-65 0.0015216269235066133 0.0 0.0 0.33886231158354513 1.601712551059593E-4 66-67 0.0015216269235066133 0.0 0.0 0.38242889297236604 1.8019266199420422E-4 68-69 0.0015616697372831032 0.0 0.0 0.43636656312929784 2.4025688265893894E-4 70-71 0.0016617767717243275 0.0 0.0 0.4973717899177801 3.203425102119186E-4 72-73 0.0018019266199420418 0.0 0.0 0.5634624540558766 5.405779859826126E-4 74-75 0.0019220550612715115 0.0 0.0 0.6301938032143968 5.605993928708575E-4 76-77 0.0019220550612715115 0.0 0.0 0.7048736509075504 5.605993928708575E-4 78-79 0.0019220550612715115 0.0 0.0 0.7934483549811457 5.605993928708575E-4 80-81 0.0021222691301539605 0.0 0.0 0.8970791570347014 5.605993928708575E-4 82-83 0.00224239757148343 0.0 0.0 1.008217986671349 6.006422066473473E-4 84-85 0.00224239757148343 0.0 0.0 1.1214590640312623 6.406850204238372E-4 86-87 0.00224239757148343 0.0 0.0 1.241527441140067 6.406850204238372E-4 88-89 0.00224239757148343 0.0 0.0 1.3820376746817697 6.80727834200327E-4 90-91 0.0024225902334776344 0.0 0.0 1.5413680306984228 7.007492410885719E-4 92-93 0.002522697267918859 0.0 0.0 1.716575362377454 7.207706479768168E-4 94-95 0.002522697267918859 0.0 0.0 1.8928838714353386 7.608134617533066E-4 96-97 0.002522697267918859 0.0 0.0 2.0767604722969804 7.608134617533066E-4 98-99 0.002722911336801308 0.0 0.0 2.2754729356628105 7.608134617533066E-4 100-101 0.002782975557466043 0.0 0.0 2.511745558350989 7.608134617533066E-4 102-103 0.0028630611850190223 0.0 0.0 2.7747067164211976 7.608134617533066E-4 104-105 0.0032835107296721655 0.0 0.0 3.0399503148766662 7.608134617533066E-4 106-107 0.003583831832995839 0.0 0.0 3.302430959181557 7.608134617533066E-4 108-109 0.003643896053660574 0.0 0.0 3.58371170455451 7.808348686415515E-4 110-111 0.0037039602743253086 0.0 0.0 3.9111618142117552 8.609204961945313E-4 112-113 0.0038641315294312677 0.0 0.0 4.2708063461452985 8.809419030827761E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4955 0.0 39.67464 1 ATCAACG 5990 0.0 33.61361 3 GTACATG 8905 0.0 33.31487 1 TCAACGC 6155 0.0 32.906075 4 CAACGCA 6220 0.0 32.657974 5 TACATGG 8745 0.0 32.15196 2 AACGCAG 6295 0.0 31.012527 6 ACATGGG 9135 0.0 30.648874 3 TATCAAC 6755 0.0 28.57228 2 GGTATCA 3560 0.0 27.61065 1 ACGCAGA 7355 0.0 25.650276 7 CGCAGAG 7295 0.0 24.469452 8 CATGGGG 4340 0.0 24.294546 4 CATGGGA 5410 0.0 22.572613 4 CGCATAG 245 4.348767E-7 21.857714 8 GTACCTG 2245 0.0 19.901583 1 GCAGAGT 8575 0.0 19.70466 9 TAGACTG 1365 0.0 19.638355 5 AGAGTAC 8155 0.0 18.382689 10-11 GTATAGG 845 0.0 18.329866 1 >>END_MODULE