FastQCFastQC Report
Thu 26 May 2016
SRR1364597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497327
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.23853504166654987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.2006545398339905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.13073978698023928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.050.032631
GTATCAA80850.045.8274081
ATCAACG97950.036.749583
TCAACGC99350.036.235354
TATCAAC103350.035.2592472
CAACGCA100850.035.137345
AACGCAG102150.034.565946
GTACATG93200.030.039791
TACATGG91800.029.641872
ACGCAGA121100.029.0094817
ACATGGG91900.028.9396653
CGCAGAG122100.028.7231258
GCAGAGT136950.024.95699
GAGTACT83600.023.68717212-13
TCGCGGA3150.023.60506882-83
AGTACTT85800.022.80215612-13
GTACTTT88450.022.1526614-15
CGCGATC3650.022.0250322-23
CAGAGTA135750.021.7679610-11
AGAGTAC133000.021.34455710-11
Produced by FastQC (version 0.11.5)